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The map label for this gene is gutB [H]

Identifier: 158337665

GI number: 158337665

Start: 4584609

End: 4585655

Strand: Reverse

Name: gutB [H]

Synonym: AM1_4548

Alternate gene names: 158337665

Gene position: 4585655-4584609 (Counterclockwise)

Preceding gene: 158337668

Following gene: 158337663

Centisome position: 70.51

GC content: 51.67

Gene sequence:

>1047_bases
GTGCTAGCAGCGGTATTACATGGCCAGACCGATCTCCGCCTAGAAACGGTTCCTGACCCTGCCCCTGAAACCGGTGAAGT
GGTGATTCAAGTGGATGTCGCGACGACCTGTGGCACGGACTTGAAAGTGTGGCGACGCGGGGGCCATGCCAAAATGCTGA
AGCCACCCACTCTGTTTGGCCATGAAGCAGCTGGTCAGATTGTAGCCATAGGCTCGGGGGTTCAGGGTTGGTCCCTTGGC
GATCGCGTCGTTGCCAACAATTCAGCACCCTGCGGACACTGCTTTTTTTGCCAGCGGCAGGAATATTCCCTTTGCACGGA
TTTAACCTTTAATAACGGCACCTTTGCCCAATATTTGAGAATTCCTGCCGCCATTGTTGAGCAAAACTTGCTCCCTATTC
CAGAACATTTATCCATGGCGACCGCCTCTCTGACTGAGCCCTTGGCCTGCGTTTTGCATGGCATTGCCCGCTCAGGTTTT
AAGCCTGAACAAAAGGATGGTCCTGCCCAAAGAGTGGTTGTGATTGGGGATGGAGCCATTGGCCTGATGTTTGTGGGCGT
TTTAGCCCATCGTGGTGCAGAGGTCATTTTATTCGGTGGGTCCGATCAGCGATTGAAATTGGGACAGGAACTCGGCGCTA
CCCATATTTTTAATCACCACCATACAGACCTAGCCGCGACCACGTTGGGACTGACAGAGAACTATGGGGCAGATGTTGTG
ATTGAAGCAACAGGAGTGCCTAGTGTTTGGGAAACCGCTATCGCCTGCGGACGACCAGGGGCAACTATCAATTTATTTGG
GGGATGTCCTCGGGATACAAGTATCACCGTCAATACTGATTTATTGCACTACAGCGAACTCACCCTGAAAGGAGTCTTCC
ACAATACCCCTACATTTGTTCGAGAATCCTTAGCCCTGTTAGCCAGTCAGGAGCTGCCGTTTGAACAACTCCTGAACGAT
ACCCAACCCCTCAATCATCTAGGGCAGGTGTTTGCTGATATGCGAGATCGCAAAACCATCAAAGCGGTCATCCTACCTCA
TACCTAA

Upstream 100 bases:

>100_bases
GATCTCAATCAGAATAAGCTAGTGCAAAACCTATCCGCCTTTTATATGACTCCAGCGATTCCCGTTAAGATGATGAAGCT
GAATAGAGGACTGAATTAAC

Downstream 100 bases:

>100_bases
CGCCCTATACTGCGACCGTCCCTGGAGGATAGATACCGGGTGACAAGGGGCGGTCGATTAACCCGCTAGCCCCTAAGATG
ACCCCACTAGAGATCTCTGC

Product: zinc binding alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MLAAVLHGQTDLRLETVPDPAPETGEVVIQVDVATTCGTDLKVWRRGGHAKMLKPPTLFGHEAAGQIVAIGSGVQGWSLG
DRVVANNSAPCGHCFFCQRQEYSLCTDLTFNNGTFAQYLRIPAAIVEQNLLPIPEHLSMATASLTEPLACVLHGIARSGF
KPEQKDGPAQRVVVIGDGAIGLMFVGVLAHRGAEVILFGGSDQRLKLGQELGATHIFNHHHTDLAATTLGLTENYGADVV
IEATGVPSVWETAIACGRPGATINLFGGCPRDTSITVNTDLLHYSELTLKGVFHNTPTFVRESLALLASQELPFEQLLND
TQPLNHLGQVFADMRDRKTIKAVILPHT

Sequences:

>Translated_348_residues
MLAAVLHGQTDLRLETVPDPAPETGEVVIQVDVATTCGTDLKVWRRGGHAKMLKPPTLFGHEAAGQIVAIGSGVQGWSLG
DRVVANNSAPCGHCFFCQRQEYSLCTDLTFNNGTFAQYLRIPAAIVEQNLLPIPEHLSMATASLTEPLACVLHGIARSGF
KPEQKDGPAQRVVVIGDGAIGLMFVGVLAHRGAEVILFGGSDQRLKLGQELGATHIFNHHHTDLAATTLGLTENYGADVV
IEATGVPSVWETAIACGRPGATINLFGGCPRDTSITVNTDLLHYSELTLKGVFHNTPTFVRESLALLASQELPFEQLLND
TQPLNHLGQVFADMRDRKTIKAVILPHT
>Mature_348_residues
MLAAVLHGQTDLRLETVPDPAPETGEVVIQVDVATTCGTDLKVWRRGGHAKMLKPPTLFGHEAAGQIVAIGSGVQGWSLG
DRVVANNSAPCGHCFFCQRQEYSLCTDLTFNNGTFAQYLRIPAAIVEQNLLPIPEHLSMATASLTEPLACVLHGIARSGF
KPEQKDGPAQRVVVIGDGAIGLMFVGVLAHRGAEVILFGGSDQRLKLGQELGATHIFNHHHTDLAATTLGLTENYGADVV
IEATGVPSVWETAIACGRPGATINLFGGCPRDTSITVNTDLLHYSELTLKGVFHNTPTFVRESLALLASQELPFEQLLND
TQPLNHLGQVFADMRDRKTIKAVILPHT

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=354, Percent_Identity=27.1186440677966, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI156523966, Length=374, Percent_Identity=25.668449197861, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4501939, Length=367, Percent_Identity=25.6130790190736, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4501933, Length=379, Percent_Identity=23.7467018469657, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788073, Length=352, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790045, Length=335, Percent_Identity=28.3582089552239, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1788075, Length=347, Percent_Identity=26.8011527377522, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787863, Length=254, Percent_Identity=31.8897637795276, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788407, Length=334, Percent_Identity=26.6467065868263, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510992, Length=334, Percent_Identity=25.1497005988024, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790718, Length=313, Percent_Identity=27.7955271565495, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786825, Length=124, Percent_Identity=34.6774193548387, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87082125, Length=365, Percent_Identity=26.3013698630137, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17562876, Length=353, Percent_Identity=28.328611898017, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17562878, Length=353, Percent_Identity=27.4787535410765, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71988145, Length=341, Percent_Identity=24.9266862170088, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=304, Percent_Identity=27.9605263157895, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562584, Length=345, Percent_Identity=25.7971014492754, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6322619, Length=330, Percent_Identity=27.5757575757576, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319955, Length=330, Percent_Identity=27.5757575757576, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323099, Length=337, Percent_Identity=24.9258160237389, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319258, Length=273, Percent_Identity=27.8388278388278, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319621, Length=275, Percent_Identity=28, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323729, Length=310, Percent_Identity=24.8387096774194, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319520, Length=349, Percent_Identity=22.0630372492837, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=247, Percent_Identity=25.5060728744939, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=117, Percent_Identity=34.1880341880342, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323961, Length=122, Percent_Identity=31.9672131147541, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17737897, Length=345, Percent_Identity=29.2753623188406, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137530, Length=344, Percent_Identity=27.6162790697674, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45551930, Length=130, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45550770, Length=130, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI221457811, Length=130, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37330; Mature: 37330

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAVLHGQTDLRLETVPDPAPETGEVVIQVDVATTCGTDLKVWRRGGHAKMLKPPTLFG
CEEEEECCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCC
HEAAGQIVAIGSGVQGWSLGDRVVANNSAPCGHCFFCQRQEYSLCTDLTFNNGTFAQYLR
CCCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHH
IPAAIVEQNLLPIPEHLSMATASLTEPLACVLHGIARSGFKPEQKDGPAQRVVVIGDGAI
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHH
GLMFVGVLAHRGAEVILFGGSDQRLKLGQELGATHIFNHHHTDLAATTLGLTENYGADVV
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHCCCCCCCCHHHEEECCCCCCCCCEE
IEATGVPSVWETAIACGRPGATINLFGGCPRDTSITVNTDLLHYSELTLKGVFHNTPTFV
EEECCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEECCEEEEEEECCCHHHH
RESLALLASQELPFEQLLNDTQPLNHLGQVFADMRDRKTIKAVILPHT
HHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
>Mature Secondary Structure
MLAAVLHGQTDLRLETVPDPAPETGEVVIQVDVATTCGTDLKVWRRGGHAKMLKPPTLFG
CEEEEECCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCC
HEAAGQIVAIGSGVQGWSLGDRVVANNSAPCGHCFFCQRQEYSLCTDLTFNNGTFAQYLR
CCCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHH
IPAAIVEQNLLPIPEHLSMATASLTEPLACVLHGIARSGFKPEQKDGPAQRVVVIGDGAI
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHH
GLMFVGVLAHRGAEVILFGGSDQRLKLGQELGATHIFNHHHTDLAATTLGLTENYGADVV
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHCCCCCCCCHHHEEECCCCCCCCCEE
IEATGVPSVWETAIACGRPGATINLFGGCPRDTSITVNTDLLHYSELTLKGVFHNTPTFV
EEECCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEECCEEEEEEECCCHHHH
RESLALLASQELPFEQLLNDTQPLNHLGQVFADMRDRKTIKAVILPHT
HHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]