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The map label for this gene is 158337636

Identifier: 158337636

GI number: 158337636

Start: 4544202

End: 4545044

Strand: Direct

Name: 158337636

Synonym: AM1_4518

Alternate gene names: NA

Gene position: 4544202-4545044 (Clockwise)

Preceding gene: 158337635

Following gene: 158337638

Centisome position: 69.87

GC content: 51.25

Gene sequence:

>843_bases
ATGATTGTTCCGTTACCAACCCTGGACTATCAATCTGCCGATGCACCACAACAGTTTGCCCTATCTTTGCAGCACTCTGG
GTTTGCGATTCTCCGCCATTGTCCAGTGGACCATGAATTAGTGGCGCATACGTACCAAGATTGGCAAACCTTTTTTGAGG
GGGATGATAAGGTCCACTATACCTTTGACCCCAAAATACAAGTGGGCTACTACCCATTTCAAACGGAACAAGCTAAGGGA
CATACCACCCCGGACCTCAAAGAGTTCTTTCATATCTATGAAGGTCATCCTCTGCCGAGGGGCATAGGCGATGCGACTTG
GAATTTATTTGACCAACTCAAGCAACTGGCCATCGAACTACTAACATGGATAGAAGCGCAAGCCCCCCTTAGCATTCGTT
CGGACTTTACAGAACCGTTAGACACCATGATTGCAGATAGTAAGGAAACTCTGTTTCGGCTCCTGCACTATCCGCCTCTA
CCCGATACCATCCCACCCGGAGCTATTCGGGCAGCGGCCCACGAAGATATCAATTTAATTACGCTATTACCTGCAGCGAC
GGGAATGGGCTTAGAGTTATTGGATGCTGAAGGGCAGTGGCAATCCGTAAAGTGCGATCGCGGCGATATTGTCGTCAATG
TTGGCGATATGCTGCAGCTTGCGAGCCAAGGGTTTTACCGCTCCACCACCCACCGTGTCGTTAATCCTGACGGTCCAGCT
CGACATCAGTCCCGGTTATCCATGCCCCTGTTTCTCCATCCCCGGCCCGAGGTGGTACTCGCAGACGGCAAAACAGCCCG
ACAGTATCTGCAAGAGCGGCTGCGGGAGATTGGCCTCGTTTAA

Upstream 100 bases:

>100_bases
AAACCATCCGTCAACGCCATCTTCAGGTCCCTTGTTATGCCGCTCAGCACCAACAAGCCGCCTATGGCACTGCCTTACTT
GCTGCCCATGGATCGCCCCT

Downstream 100 bases:

>100_bases
GGACATCACACCTATTAATGTTGAGGGTTTCATACCTATTAATTCGGGGGAGCGATACGGGTCGCAAAATTTTGTGATCG
CGTTGGTGACAGCTGGCGCG

Product: 2OG-Fe(II) oxygenase family oxidoreductase

Products: isopenicillin N; H2O

Alternate protein names: Oxidoreductase; 2OG-Fe(II) Oxygenase Family Oxidoreductase; Isopenicillin N Synthase; Oxidoreductase 2OG-Fe(II) Oxygenase Family; Iron/Ascorbate Family Oxidoreductase; 2OG-Fe(II) Oxygenase Superfamily Protein; Flavonol Synthase/Dioxygenase; 2og-Fe(Ii) Oxygenase; Oxidoreductase Iron/Ascorbate Family Protein; Iron/Ascorbate Oxidoreductase; 2-Oxobutyrate Oxidase; 1-Aminocyclopropane-1-Carboxylate Oxidase; Isopenicillin N Synthase And Related Dioxygenases; Oxidoreductase 2og-Fe(Ii) Oxygenase Family; Iron/Ascorbate-Dependent Oxidoreductase Family Protein; Oxidoreductase Iron/Ascorbate Family; Fe-Containing Oxidoreductase; Isopenicillin-N Synthase; Flavonol Synthase/Flavanone 3-Hydroxylase; Oxygenase/Oxidoreductase; Iron/Ascorbate Oxidoreductase Family Protein

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MIVPLPTLDYQSADAPQQFALSLQHSGFAILRHCPVDHELVAHTYQDWQTFFEGDDKVHYTFDPKIQVGYYPFQTEQAKG
HTTPDLKEFFHIYEGHPLPRGIGDATWNLFDQLKQLAIELLTWIEAQAPLSIRSDFTEPLDTMIADSKETLFRLLHYPPL
PDTIPPGAIRAAAHEDINLITLLPAATGMGLELLDAEGQWQSVKCDRGDIVVNVGDMLQLASQGFYRSTTHRVVNPDGPA
RHQSRLSMPLFLHPRPEVVLADGKTARQYLQERLREIGLV

Sequences:

>Translated_280_residues
MIVPLPTLDYQSADAPQQFALSLQHSGFAILRHCPVDHELVAHTYQDWQTFFEGDDKVHYTFDPKIQVGYYPFQTEQAKG
HTTPDLKEFFHIYEGHPLPRGIGDATWNLFDQLKQLAIELLTWIEAQAPLSIRSDFTEPLDTMIADSKETLFRLLHYPPL
PDTIPPGAIRAAAHEDINLITLLPAATGMGLELLDAEGQWQSVKCDRGDIVVNVGDMLQLASQGFYRSTTHRVVNPDGPA
RHQSRLSMPLFLHPRPEVVLADGKTARQYLQERLREIGLV
>Mature_280_residues
MIVPLPTLDYQSADAPQQFALSLQHSGFAILRHCPVDHELVAHTYQDWQTFFEGDDKVHYTFDPKIQVGYYPFQTEQAKG
HTTPDLKEFFHIYEGHPLPRGIGDATWNLFDQLKQLAIELLTWIEAQAPLSIRSDFTEPLDTMIADSKETLFRLLHYPPL
PDTIPPGAIRAAAHEDINLITLLPAATGMGLELLDAEGQWQSVKCDRGDIVVNVGDMLQLASQGFYRSTTHRVVNPDGPA
RHQSRLSMPLFLHPRPEVVLADGKTARQYLQERLREIGLV

Specific function: Unknown

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Drosophila melanogaster, GI85816264, Length=276, Percent_Identity=27.1739130434783, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28572124, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.21.3.1

Molecular weight: Translated: 31627; Mature: 31627

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVPLPTLDYQSADAPQQFALSLQHSGFAILRHCPVDHELVAHTYQDWQTFFEGDDKVHY
CCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
TFDPKIQVGYYPFQTEQAKGHTTPDLKEFFHIYEGHPLPRGIGDATWNLFDQLKQLAIEL
EECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LTWIEAQAPLSIRSDFTEPLDTMIADSKETLFRLLHYPPLPDTIPPGAIRAAAHEDINLI
HHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
TLLPAATGMGLELLDAEGQWQSVKCDRGDIVVNVGDMLQLASQGFYRSTTHRVVNPDGPA
EEECCCCCCCEEEECCCCCCCEEECCCCCEEEEHHHHHHHHHCCCCCCCCCEEECCCCCC
RHQSRLSMPLFLHPRPEVVLADGKTARQYLQERLREIGLV
HHHHHCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVPLPTLDYQSADAPQQFALSLQHSGFAILRHCPVDHELVAHTYQDWQTFFEGDDKVHY
CCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
TFDPKIQVGYYPFQTEQAKGHTTPDLKEFFHIYEGHPLPRGIGDATWNLFDQLKQLAIEL
EECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LTWIEAQAPLSIRSDFTEPLDTMIADSKETLFRLLHYPPLPDTIPPGAIRAAAHEDINLI
HHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
TLLPAATGMGLELLDAEGQWQSVKCDRGDIVVNVGDMLQLASQGFYRSTTHRVVNPDGPA
EEECCCCCCCEEEECCCCCCCEEECCCCCEEEEHHHHHHHHHCCCCCCCCCEEECCCCCC
RHQSRLSMPLFLHPRPEVVLADGKTARQYLQERLREIGLV
HHHHHCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine; O2

Specific reaction: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA