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The map label for this gene is aspS

Identifier: 158337540

GI number: 158337540

Start: 4446190

End: 4447977

Strand: Reverse

Name: aspS

Synonym: AM1_4421

Alternate gene names: 158337540

Gene position: 4447977-4446190 (Counterclockwise)

Preceding gene: 158337541

Following gene: 158337539

Centisome position: 68.39

GC content: 49.33

Gene sequence:

>1788_bases
ATGCGAACCCACTATTGCAGTGAATTACGAAATGATCATGTGGGTGAACGGGTTACCCTCTGTGGCTGGGTAGATCGCCG
TCGAGATCATGGTGGCGTGATTTTTTTGGACGTGCGCGATCGAACGGGTCTGATCCAAATTGTCAGTGACCCGGAACGAA
CCCCTGAAGCCTATCCCCAAGCAGATGCCCTCCGAAATGAGTATGTGGTTCGGATTCATGGTGAGGTCAGTCGTCGTCCG
GATGATTCCCTCAATCCTCGCTTGCCCACCGGAGAAGTTGAAATCTACGCTGATCAGATTGAGCTGCTCAATGCGGTTCA
CCAGCAGTTGCCCTTTCAGGTCTCTACGGCTGATGTGGAATCGGTCAAAGAAGAGCTGCGCCTCAAGTATCGGTATTTGG
ATTTGCGACGCGATCGCATGCATCAAAACATCATGATGCGGCATCGGTTAATTCAATCGATTCGTCGCTATTTGGAAGAT
CAGCAGAACTTCGTAGATATTGAAACCCCCGTCTTAACTCGCTCCACCCCTGAAGGCGCACGAGACTACTTGGTACCCAG
TCGAGCCAATCCGGGAGAATGGTTTGCCCTGCCTCAGTCACCTCAGATTTTTAAGCAATTGCTGATGGTGTCTGGGTTTG
ATCGCTATTACCAAATTGCCCGCTGTTTCCGGGATGAGGACTTACGAGCCGACCGCCAACCTGAATTTACGCAGCTGGAC
ATGGAAATGAGCTTCATGTCCCAAGAGGAAATCATTCAGCTCAATGAAGACTTAATGCGGCATGTCTTCAAGACCCTCAA
AGATATTGACTTGCCGAAGACATTCCCACAGATGACCTATGCCGAAGCCATGGAACGCTTTGGCACCGATCGTCCAGATA
CCCGGTTTGATCTGGAGTTAGTCAATGTTTCTGATCTGCTCGAAGATTCAGGATTCAAGGTGTTCTCAGGGGCGATTAAA
AAAGGCGGACAAGTTAAAGTTCTACCTATCCCTGATGGCAATGCCAAGATTTCCAACGTTCGCATTAAACCCGGTGGTGA
TTTATTCTCTGAAGCCAGTGCAGGTGGGGCTAAAGGTCTTGCCTATATCCGGGTTAAAGACGATGGTGCGATTGATACGA
TTGGAGCCATTAAAGATAATCTATCACCAGAGCAAACAGCAGAATTGCTAGAGCGAACGGGAGCCAAGCCAGGTCATTTA
CTCCTGTTTGGCGCAGGTCCCACAGAAATTGTGAACGCCTCTCTCTCCCGATTACGATTGGCATTGGGCGCAGAAATGAA
GCTGATTGATCCGGACCAAATCGATCTGCTATGGATTACCGAATTCCCCATGTTTGAGTGGAACGCCGATGAAAAGAGGC
TTGAGGCGCTCCACCACCCCTTTACAGCGCCTTACCCTGAAGATGAAGCGGATCTAAAAACGGCCCGTGCCCAAGCCTAT
GACTTGGTCTATAACGGCTTAGAAATTGGCGGCGGGAGCCTGAGGATTTATCAAGCAGATTTACAACGGCGAGTGTTTGA
CGCCATTGGCTTATCAGAGGAAGAAGCTCAGGATAAATTTGGGTTCTTGCTGAATGCCTTTAACTTTGGTGCTCCCCCCC
ACGGTGGGATTGCCTATGGCATTGATCGTTTGGCGATGCTCCTCAGTGGTGAAGAATCGATTCGAGACACAATTGCATTT
CCCAAAACCCAGCAGGCTCGCTGTTTGCTAACACAAGCACCTTCCAGTGTGGATCAGAAGCAGCTTAAAGAATTGCAGAT
CAAATCCACGGCTGAACCTAAAAATTAG

Upstream 100 bases:

>100_bases
TAATAGCTGCGATAATAGGTGAGTTTGCCAAGGTGCAATGATAATTTCCTCCTGAGTTGGGGAATCTTGTTGTTTGAAGA
GGGAGCTGGATCAATCAGGT

Downstream 100 bases:

>100_bases
TGGTCATCCTGCTGCTCCAACCGTAAAAACCATCTGTAAGGGACATCTCTTGATCCGCTTAAAATCCAAACGGCCTGGCC
TGAATTGAACCCTACTCTTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MRTHYCSELRNDHVGERVTLCGWVDRRRDHGGVIFLDVRDRTGLIQIVSDPERTPEAYPQADALRNEYVVRIHGEVSRRP
DDSLNPRLPTGEVEIYADQIELLNAVHQQLPFQVSTADVESVKEELRLKYRYLDLRRDRMHQNIMMRHRLIQSIRRYLED
QQNFVDIETPVLTRSTPEGARDYLVPSRANPGEWFALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQLD
MEMSFMSQEEIIQLNEDLMRHVFKTLKDIDLPKTFPQMTYAEAMERFGTDRPDTRFDLELVNVSDLLEDSGFKVFSGAIK
KGGQVKVLPIPDGNAKISNVRIKPGGDLFSEASAGGAKGLAYIRVKDDGAIDTIGAIKDNLSPEQTAELLERTGAKPGHL
LLFGAGPTEIVNASLSRLRLALGAEMKLIDPDQIDLLWITEFPMFEWNADEKRLEALHHPFTAPYPEDEADLKTARAQAY
DLVYNGLEIGGGSLRIYQADLQRRVFDAIGLSEEEAQDKFGFLLNAFNFGAPPHGGIAYGIDRLAMLLSGEESIRDTIAF
PKTQQARCLLTQAPSSVDQKQLKELQIKSTAEPKN

Sequences:

>Translated_595_residues
MRTHYCSELRNDHVGERVTLCGWVDRRRDHGGVIFLDVRDRTGLIQIVSDPERTPEAYPQADALRNEYVVRIHGEVSRRP
DDSLNPRLPTGEVEIYADQIELLNAVHQQLPFQVSTADVESVKEELRLKYRYLDLRRDRMHQNIMMRHRLIQSIRRYLED
QQNFVDIETPVLTRSTPEGARDYLVPSRANPGEWFALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQLD
MEMSFMSQEEIIQLNEDLMRHVFKTLKDIDLPKTFPQMTYAEAMERFGTDRPDTRFDLELVNVSDLLEDSGFKVFSGAIK
KGGQVKVLPIPDGNAKISNVRIKPGGDLFSEASAGGAKGLAYIRVKDDGAIDTIGAIKDNLSPEQTAELLERTGAKPGHL
LLFGAGPTEIVNASLSRLRLALGAEMKLIDPDQIDLLWITEFPMFEWNADEKRLEALHHPFTAPYPEDEADLKTARAQAY
DLVYNGLEIGGGSLRIYQADLQRRVFDAIGLSEEEAQDKFGFLLNAFNFGAPPHGGIAYGIDRLAMLLSGEESIRDTIAF
PKTQQARCLLTQAPSSVDQKQLKELQIKSTAEPKN
>Mature_595_residues
MRTHYCSELRNDHVGERVTLCGWVDRRRDHGGVIFLDVRDRTGLIQIVSDPERTPEAYPQADALRNEYVVRIHGEVSRRP
DDSLNPRLPTGEVEIYADQIELLNAVHQQLPFQVSTADVESVKEELRLKYRYLDLRRDRMHQNIMMRHRLIQSIRRYLED
QQNFVDIETPVLTRSTPEGARDYLVPSRANPGEWFALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQLD
MEMSFMSQEEIIQLNEDLMRHVFKTLKDIDLPKTFPQMTYAEAMERFGTDRPDTRFDLELVNVSDLLEDSGFKVFSGAIK
KGGQVKVLPIPDGNAKISNVRIKPGGDLFSEASAGGAKGLAYIRVKDDGAIDTIGAIKDNLSPEQTAELLERTGAKPGHL
LLFGAGPTEIVNASLSRLRLALGAEMKLIDPDQIDLLWITEFPMFEWNADEKRLEALHHPFTAPYPEDEADLKTARAQAY
DLVYNGLEIGGGSLRIYQADLQRRVFDAIGLSEEEAQDKFGFLLNAFNFGAPPHGGIAYGIDRLAMLLSGEESIRDTIAF
PKTQQARCLLTQAPSSVDQKQLKELQIKSTAEPKN

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:
GO:0000166: Aspartyl-tRNA synthetase
GO:0003676: Aspartyl-tRNA synthetase
GO:0004812: Aspartyl-tRNA synthetase
GO:0004815: Aspartyl-tRNA synthetase
GO:0005524: Aspartyl-tRNA synthetase
GO:0005737: Aspartyl-tRNA synthetase
GO:0006412: Aspartyl-tRNA synthetase
GO:0006418: Aspartyl-tRNA synthetase
GO:0016874: Aspartyl-tRNA synthetase

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=40.0329489291598, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI5031815, Length=312, Percent_Identity=25, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI194272210, Length=312, Percent_Identity=25, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI45439306, Length=264, Percent_Identity=25, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788173, Length=602, Percent_Identity=47.6744186046512, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1790571, Length=314, Percent_Identity=26.1146496815287, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=603, Percent_Identity=34.1625207296849, Blast_Score=332, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=270, Percent_Identity=25.5555555555556, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=270, Percent_Identity=25.5555555555556, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=270, Percent_Identity=25.5555555555556, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=282, Percent_Identity=23.758865248227, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=660, Percent_Identity=30.7575757575758, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6320242, Length=316, Percent_Identity=25, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=248, Percent_Identity=28.2258064516129, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324256, Length=194, Percent_Identity=25.7731958762887, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=275, Percent_Identity=27.6363636363636, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584738, Length=613, Percent_Identity=37.0309951060359, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640849, Length=258, Percent_Identity=24.031007751938, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24640851, Length=258, Percent_Identity=24.031007751938, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17136276, Length=189, Percent_Identity=29.6296296296296, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_ACAM1 (B0CF01)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518715.1
- GeneID:   5683223
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4421
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   AMAR329726:AM1_4421-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67537; Mature: 67537

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHYCSELRNDHVGERVTLCGWVDRRRDHGGVIFLDVRDRTGLIQIVSDPERTPEAYPQ
CCCHHHHHHHCCCCCCEEEEEEHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCC
ADALRNEYVVRIHGEVSRRPDDSLNPRLPTGEVEIYADQIELLNAVHQQLPFQVSTADVE
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEHHHHH
SVKEELRLKYRYLDLRRDRMHQNIMMRHRLIQSIRRYLEDQQNFVDIETPVLTRSTPEGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCC
RDYLVPSRANPGEWFALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQLD
HHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEC
MEMSFMSQEEIIQLNEDLMRHVFKTLKDIDLPKTFPQMTYAEAMERFGTDRPDTRFDLEL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
VNVSDLLEDSGFKVFSGAIKKGGQVKVLPIPDGNAKISNVRIKPGGDLFSEASAGGAKGL
ECHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHCCCCCCCCE
AYIRVKDDGAIDTIGAIKDNLSPEQTAELLERTGAKPGHLLLFGAGPTEIVNASLSRLRL
EEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
ALGAEMKLIDPDQIDLLWITEFPMFEWNADEKRLEALHHPFTAPYPEDEADLKTARAQAY
HHCCCEEEECCCCEEEEEEECCCCEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DLVYNGLEIGGGSLRIYQADLQRRVFDAIGLSEEEAQDKFGFLLNAFNFGAPPHGGIAYG
HHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IDRLAMLLSGEESIRDTIAFPKTQQARCLLTQAPSSVDQKQLKELQIKSTAEPKN
HHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRTHYCSELRNDHVGERVTLCGWVDRRRDHGGVIFLDVRDRTGLIQIVSDPERTPEAYPQ
CCCHHHHHHHCCCCCCEEEEEEHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCC
ADALRNEYVVRIHGEVSRRPDDSLNPRLPTGEVEIYADQIELLNAVHQQLPFQVSTADVE
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEHHHHH
SVKEELRLKYRYLDLRRDRMHQNIMMRHRLIQSIRRYLEDQQNFVDIETPVLTRSTPEGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCC
RDYLVPSRANPGEWFALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQLD
HHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEC
MEMSFMSQEEIIQLNEDLMRHVFKTLKDIDLPKTFPQMTYAEAMERFGTDRPDTRFDLEL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
VNVSDLLEDSGFKVFSGAIKKGGQVKVLPIPDGNAKISNVRIKPGGDLFSEASAGGAKGL
ECHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHCCCCCCCCE
AYIRVKDDGAIDTIGAIKDNLSPEQTAELLERTGAKPGHLLLFGAGPTEIVNASLSRLRL
EEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
ALGAEMKLIDPDQIDLLWITEFPMFEWNADEKRLEALHHPFTAPYPEDEADLKTARAQAY
HHCCCEEEECCCCEEEEEEECCCCEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DLVYNGLEIGGGSLRIYQADLQRRVFDAIGLSEEEAQDKFGFLLNAFNFGAPPHGGIAYG
HHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IDRLAMLLSGEESIRDTIAFPKTQQARCLLTQAPSSVDQKQLKELQIKSTAEPKN
HHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA