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The map label for this gene is groEL

Identifier: 158337530

GI number: 158337530

Start: 4437180

End: 4438811

Strand: Reverse

Name: groEL

Synonym: AM1_4411

Alternate gene names: 158337530

Gene position: 4438811-4437180 (Counterclockwise)

Preceding gene: 158337531

Following gene: 158337521

Centisome position: 68.25

GC content: 49.69

Gene sequence:

>1632_bases
ATGGCTAAACGCATTATTTATAACGAGAATGCTCGTCGCGCCCTTGAGAAAGGGATGGATATTCTATGTGAATCTGTTGC
GGTGACATTAGGCCCTAAAGGTCGCAATGTTGTTCTAGAAAAGAAATTTGGTGCCCCTCAGATTGTCAATGATGGTGTCA
CCATTGCTAAGGAAATTGAACTAGAAGACAACATTGAGAACACAGGGGTTGCCCTGATTCGTCAAGCGGCTTCCAAAACG
AATGATGCCGCAGGAGATGGCACCACAACAGCTACCGTCCTTGCCCATGCCATGGTCAAAGAAGGGATGCGCAACGTAGT
TGCGGGTGCGAATGCAATTTCCCTCAAGCGTGGCATTGAGAAAGCTGCTGGCTTTCTTGTAGAGAAAATCGCTGAGAATG
CTCGCTCTGTTGAAGATTCCACCGCCATTGCTCAAGTGGGAACGATTTCTGCAGGTAATGACGATGAAGTCGGCCAAATG
ATTGCCAATGCGATGGATAAGGTTGGCAAAGAAGGCGTTATTTCCCTTGAAGAAGGCAAGTCCATGACCACCGAATTGGA
GGTCACTGAAGGGATGCGCTTTGAGAAAGGCTATATTTCTCCTTATTTCGCGACTGACACAGAGCGGATGGAAGCTGTGC
TAGACGAGCCCTACATCCTACTGACCGACAAGAAAATTACCCTCGTGCAAGATTTAGTGCCTGTACTCGAGCAGGCTGCA
CGGGCAGGTAAGCCCTTGTTGGTGATTGCTGAGGATATTGAGAAAGAAGCCTTAGCCACCCTCGTCGTCAACCGTTTACG
GGGTGTGTTGAATGTAGCTGCCGTGAAAGCACCTGGCTTCGGCGATCGTCGTAAAGCCATGCTGGAAGATATTGCAGTCT
TGACTGGTGGTCAGGTGATTACTGAAGATGCAGGTCTCAAACTAGAGACTGCCAAGCTAGAAATGATGGGACAAGCTCGT
CGCATCACCATCACGAAAGACACCACTACCCTTGTTGCCGAAGGCAATGAGGCCGACGTCCAAGCTCGCTGCGAGCAAAT
TCGTCGTCAAATGGATGAGACCGAGTCTTCCTACGATAAAGAGAAGTTGCAAGAGCGCTTGGCTAAATTGGCCGGTGGTG
TTGCGGTGATCAAAGTCGGTGCTGCCACTGAAACTGAGATGAAGGATCGAAAGCTCCGCCTAGAAGATGCCATCAACTCC
ACTAAAGCGGCTGTTGAAGAAGGGATTGTTCCCGGTGGTGGAACCACCTTGGCTCATCTTGGTCCTCAACTTGCAAGTTG
GGCTGCGGATAACTTAACTGGCGAAGAGCTGATTGGTGCCACTATCGTTGAACGGGCCTTAACTGCACCTCTAAAGCGCA
TTGCTGAGAATGCTGGCCAAAATGGTGCCGTTATCGCTGAACGCGTTCGTGAAAAAGAGTTCAATGTCGGTTTTGATGCG
TCAGTGAATGAGTTCACGGATATGTTTGCCGCTGGCATTGTCGATCCTGCAAAGGTGACTCGTTCTGCGCTGCAAAATGC
CGCTTCTATTGCTGGTATGGTCCTCACCACTGAGTGCATTATTTCTGATAAGCCTGAGCCGAAGGAAAATGCTCCTACCG
GTGCTGGCATGGGTGGAGACTTCGATTACTAG

Upstream 100 bases:

>100_bases
AAGCGTAATATCATCCTTCGATTCGTTCTGCCCATTCAACTGGGCAATTTTATTTTGTCTAACTTCGCTGTTACTCCTTT
AAGTTTGAGGTTGAGCTTCC

Downstream 100 bases:

>100_bases
AGTCCCAAGCCTAATTGATTAGACCCAGAGGGCGGTGTTTACCGCCCTCTTTTTTGTTCTTGCCTCAGATTCAGTTATTG
GAAATAAAAGGCCACAAAAA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MAKRIIYNENARRALEKGMDILCESVAVTLGPKGRNVVLEKKFGAPQIVNDGVTIAKEIELEDNIENTGVALIRQAASKT
NDAAGDGTTTATVLAHAMVKEGMRNVVAGANAISLKRGIEKAAGFLVEKIAENARSVEDSTAIAQVGTISAGNDDEVGQM
IANAMDKVGKEGVISLEEGKSMTTELEVTEGMRFEKGYISPYFATDTERMEAVLDEPYILLTDKKITLVQDLVPVLEQAA
RAGKPLLVIAEDIEKEALATLVVNRLRGVLNVAAVKAPGFGDRRKAMLEDIAVLTGGQVITEDAGLKLETAKLEMMGQAR
RITITKDTTTLVAEGNEADVQARCEQIRRQMDETESSYDKEKLQERLAKLAGGVAVIKVGAATETEMKDRKLRLEDAINS
TKAAVEEGIVPGGGTTLAHLGPQLASWAADNLTGEELIGATIVERALTAPLKRIAENAGQNGAVIAERVREKEFNVGFDA
SVNEFTDMFAAGIVDPAKVTRSALQNAASIAGMVLTTECIISDKPEPKENAPTGAGMGGDFDY

Sequences:

>Translated_543_residues
MAKRIIYNENARRALEKGMDILCESVAVTLGPKGRNVVLEKKFGAPQIVNDGVTIAKEIELEDNIENTGVALIRQAASKT
NDAAGDGTTTATVLAHAMVKEGMRNVVAGANAISLKRGIEKAAGFLVEKIAENARSVEDSTAIAQVGTISAGNDDEVGQM
IANAMDKVGKEGVISLEEGKSMTTELEVTEGMRFEKGYISPYFATDTERMEAVLDEPYILLTDKKITLVQDLVPVLEQAA
RAGKPLLVIAEDIEKEALATLVVNRLRGVLNVAAVKAPGFGDRRKAMLEDIAVLTGGQVITEDAGLKLETAKLEMMGQAR
RITITKDTTTLVAEGNEADVQARCEQIRRQMDETESSYDKEKLQERLAKLAGGVAVIKVGAATETEMKDRKLRLEDAINS
TKAAVEEGIVPGGGTTLAHLGPQLASWAADNLTGEELIGATIVERALTAPLKRIAENAGQNGAVIAERVREKEFNVGFDA
SVNEFTDMFAAGIVDPAKVTRSALQNAASIAGMVLTTECIISDKPEPKENAPTGAGMGGDFDY
>Mature_542_residues
AKRIIYNENARRALEKGMDILCESVAVTLGPKGRNVVLEKKFGAPQIVNDGVTIAKEIELEDNIENTGVALIRQAASKTN
DAAGDGTTTATVLAHAMVKEGMRNVVAGANAISLKRGIEKAAGFLVEKIAENARSVEDSTAIAQVGTISAGNDDEVGQMI
ANAMDKVGKEGVISLEEGKSMTTELEVTEGMRFEKGYISPYFATDTERMEAVLDEPYILLTDKKITLVQDLVPVLEQAAR
AGKPLLVIAEDIEKEALATLVVNRLRGVLNVAAVKAPGFGDRRKAMLEDIAVLTGGQVITEDAGLKLETAKLEMMGQARR
ITITKDTTTLVAEGNEADVQARCEQIRRQMDETESSYDKEKLQERLAKLAGGVAVIKVGAATETEMKDRKLRLEDAINST
KAAVEEGIVPGGGTTLAHLGPQLASWAADNLTGEELIGATIVERALTAPLKRIAENAGQNGAVIAERVREKEFNVGFDAS
VNEFTDMFAAGIVDPAKVTRSALQNAASIAGMVLTTECIISDKPEPKENAPTGAGMGGDFDY

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=44.4022770398482, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=44.4022770398482, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI1790586, Length=526, Percent_Identity=55.893536121673, Blast_Score=586, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=44.6124763705104, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI193210679, Length=215, Percent_Identity=42.7906976744186, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=50, Blast_Score=505, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=46.2998102466793, Blast_Score=470, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=46.2998102466793, Blast_Score=470, Evalue=1e-132,
Organism=Drosophila melanogaster, GI45550936, Length=526, Percent_Identity=43.9163498098859, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI45550132, Length=526, Percent_Identity=43.9163498098859, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI45550935, Length=526, Percent_Identity=43.9163498098859, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI17864606, Length=543, Percent_Identity=38.8581952117864, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24584129, Length=532, Percent_Identity=36.0902255639098, Blast_Score=309, Evalue=4e-84,
Organism=Drosophila melanogaster, GI19921262, Length=532, Percent_Identity=36.0902255639098, Blast_Score=309, Evalue=4e-84,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH602_ACAM1 (B0CEZ1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518705.1
- ProteinModelPortal:   B0CEZ1
- SMR:   B0CEZ1
- GeneID:   5683213
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4411
- HOGENOM:   HBG625289
- OMA:   KPFNDGF
- ProtClustDB:   PRK00013
- BioCyc:   AMAR329726:AM1_4411-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57841; Mature: 57709

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRIIYNENARRALEKGMDILCESVAVTLGPKGRNVVLEKKFGAPQIVNDGVTIAKEIE
CCCCEEECCHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCEECCCCEEEEEEC
LEDNIENTGVALIRQAASKTNDAAGDGTTTATVLAHAMVKEGMRNVVAGANAISLKRGIE
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAAGFLVEKIAENARSVEDSTAIAQVGTISAGNDDEVGQMIANAMDKVGKEGVISLEEGK
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
SMTTELEVTEGMRFEKGYISPYFATDTERMEAVLDEPYILLTDKKITLVQDLVPVLEQAA
CCEEEEEHHCCCEECCCCCCCEECCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHH
RAGKPLLVIAEDIEKEALATLVVNRLRGVLNVAAVKAPGFGDRRKAMLEDIAVLTGGQVI
HCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEE
TEDAGLKLETAKLEMMGQARRITITKDTTTLVAEGNEADVQARCEQIRRQMDETESSYDK
ECCCCCEEEHHHHHHCCCCEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCH
EKLQERLAKLAGGVAVIKVGAATETEMKDRKLRLEDAINSTKAAVEEGIVPGGGTTLAHL
HHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
GPQLASWAADNLTGEELIGATIVERALTAPLKRIAENAGQNGAVIAERVREKEFNVGFDA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SVNEFTDMFAAGIVDPAKVTRSALQNAASIAGMVLTTECIISDKPEPKENAPTGAGMGGD
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FDY
CCC
>Mature Secondary Structure 
AKRIIYNENARRALEKGMDILCESVAVTLGPKGRNVVLEKKFGAPQIVNDGVTIAKEIE
CCCEEECCHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCEECCCCEEEEEEC
LEDNIENTGVALIRQAASKTNDAAGDGTTTATVLAHAMVKEGMRNVVAGANAISLKRGIE
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAAGFLVEKIAENARSVEDSTAIAQVGTISAGNDDEVGQMIANAMDKVGKEGVISLEEGK
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
SMTTELEVTEGMRFEKGYISPYFATDTERMEAVLDEPYILLTDKKITLVQDLVPVLEQAA
CCEEEEEHHCCCEECCCCCCCEECCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHH
RAGKPLLVIAEDIEKEALATLVVNRLRGVLNVAAVKAPGFGDRRKAMLEDIAVLTGGQVI
HCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEE
TEDAGLKLETAKLEMMGQARRITITKDTTTLVAEGNEADVQARCEQIRRQMDETESSYDK
ECCCCCEEEHHHHHHCCCCEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCH
EKLQERLAKLAGGVAVIKVGAATETEMKDRKLRLEDAINSTKAAVEEGIVPGGGTTLAHL
HHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
GPQLASWAADNLTGEELIGATIVERALTAPLKRIAENAGQNGAVIAERVREKEFNVGFDA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SVNEFTDMFAAGIVDPAKVTRSALQNAASIAGMVLTTECIISDKPEPKENAPTGAGMGGD
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FDY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA