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The map label for this gene is kefA [C]

Identifier: 158337494

GI number: 158337494

Start: 4402324

End: 4404051

Strand: Direct

Name: kefA [C]

Synonym: AM1_4373

Alternate gene names: 158337494

Gene position: 4402324-4404051 (Clockwise)

Preceding gene: 158337493

Following gene: 158337499

Centisome position: 67.69

GC content: 45.2

Gene sequence:

>1728_bases
ATGACTCTATTCGCTGTTGATGTTCATTTCCTCAAGAACCTATTCATTGTTGTGGGGATCTTGGGAGGAGCTGCGATCTC
AGATTGGTTGATGGGGCGCTGGATGCAGGGGCGTTTGAGAACGGCTCCCAAAAAAGGTCGAGCGATTTACCCCCTTTTAC
GACGGTGTTTGCTGTTTGGGTTAAGACTCGGGATTTGGCTAGTCGCCTTTTACAATATCTTGACGCTCATCCCTGCCCTC
CAACCTATTAGACAGTTGTTTATCACTGGTCTGGCGGCTTTTCCTGACAAGTTTTGGGGAGCGTTGAATACCCCTTTTAC
GGATGTCGGAGAACGGAAGAATCCGATCTCCTTGTTGACGCTCATCATCTTTGTCTCAATCACTGTTTTGGTTTTTGTGG
GCGCTCGGTTATGTGGTCAGTGGCTTAAGAAGAGCATTCTGCCCCAGACTCGTATGGAGCGGGGAGCTCAAGCCGCCATC
TCTACTATCATCAGCTATACCTTTGGTCTGATTGGCTTCATCATTCTTCTCCAATCGGTAGGGATAGATCTGAGCTCATT
GGCTGTTATTGCTGGTGTTTTGGGTTTGGGTTTAGGTTTTGGTTTGCAAGAGCTAGCCAGTAACTTCGTGAGTGGTTTGA
CCCTATTACTGGAACAGCAAATCCGGGTCGGAGATTTTGTAGAAGTAGATGGTTTGTTAGGGACTATTGAGCGAATTTCT
ATTCGTTCGACGATTGTTCGGACTCAAGATCGGCTGTTTGTTGTAGTGCCTAATCAACGGTTTTTTGCTAAAAACGTCAT
CAATTGGACTTACCAAACGCCAGAAAGTCGCTTGCATATTCCTGTGAGTGTTGCCTATGGTACCGATACGGTTTTAGTGA
CAGAAGCCTTATTGATTGCAGCGCGGTCAGAATCGCGAGTTTTAAAGTACCCTCCTCCGCAGGTATGGTTCAAATCTTTT
GGGGATGATGCTTATAAATTTGAATTATTGGCATGGATAAACCAGCCTTGTGATTTTGAACCTATCAAGAGTTCACTCAA
TTTTTTGATTGAACAAGAGTTAAATCGTAAGAACATCCAAATCCCTTTTCCCCAACGAGAGCTGTGGTTAAAGAATCCTG
AAGCGTTAGCCCAGGCTTTACAACCTCAACCAGAGGCTGCTGCTCCACTGCCAACGGTTGCTAGCGGGGAGGGGCTCAAA
CTCAAAACGGGCATTAAAGTCACGAAGAACAGCACCTTACGGGTCTTGTTGCGTAAGGTCAGCTATTTTGAAAACTGTAC
GAATGCTCAATTGAGAGTATTAATTGAACAAGGATATCGACAGTTTTATGCTCCTGAACAAGTGATTTTTCGGGAAAATG
AATCAGGTGAGTCCTTTTACGTTATCCTTTCAGGCCAAGTAGAAGTCTATTCTCAAAAACTCAATCGCCAAATCGCCATC
TTAGGTGTTGGCGACTTTTTTGGTGAGATTTCTTTATTAACCGGGGCACCACGAACGGCAACCATGCGGGTTCTTGAAGC
GACAACCTTATTTGTAGTCGATCGACAAGCGTTACAAAAATTACTCCAGAATTACAAAGCTCTAGCCGAAGAGATTGCAA
AGTCTCTCTCTCAGAGGCAACAAGTGCTTGAAGAATTGGGCCTGACGCAGATTAATCCATCAGAATCGGCGGGGGACGAT
CCCTTTATCTGGATCCGTCGTCGTATTCAGACCTTGTTTGGGATATAA

Upstream 100 bases:

>100_bases
CCTACAGCGCTATTCAATTGCATAATCCTAAGGAAAGGGGTGAGTTGAGCTCACCCCTTTCTGCTGTCTATAATTTGTGC
GGTCTAAATTATTGAGGCAA

Downstream 100 bases:

>100_bases
AAAGACATCCAAAGGGAGGCATTATCTGTTGATTATTTGCTTGCCCGGCATGATTAGGTCTGAAGTAGGCATTGAGAGGC
TTGTAAGTCTGTTATCGTTG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MTLFAVDVHFLKNLFIVVGILGGAAISDWLMGRWMQGRLRTAPKKGRAIYPLLRRCLLFGLRLGIWLVAFYNILTLIPAL
QPIRQLFITGLAAFPDKFWGALNTPFTDVGERKNPISLLTLIIFVSITVLVFVGARLCGQWLKKSILPQTRMERGAQAAI
STIISYTFGLIGFIILLQSVGIDLSSLAVIAGVLGLGLGFGLQELASNFVSGLTLLLEQQIRVGDFVEVDGLLGTIERIS
IRSTIVRTQDRLFVVVPNQRFFAKNVINWTYQTPESRLHIPVSVAYGTDTVLVTEALLIAARSESRVLKYPPPQVWFKSF
GDDAYKFELLAWINQPCDFEPIKSSLNFLIEQELNRKNIQIPFPQRELWLKNPEALAQALQPQPEAAAPLPTVASGEGLK
LKTGIKVTKNSTLRVLLRKVSYFENCTNAQLRVLIEQGYRQFYAPEQVIFRENESGESFYVILSGQVEVYSQKLNRQIAI
LGVGDFFGEISLLTGAPRTATMRVLEATTLFVVDRQALQKLLQNYKALAEEIAKSLSQRQQVLEELGLTQINPSESAGDD
PFIWIRRRIQTLFGI

Sequences:

>Translated_575_residues
MTLFAVDVHFLKNLFIVVGILGGAAISDWLMGRWMQGRLRTAPKKGRAIYPLLRRCLLFGLRLGIWLVAFYNILTLIPAL
QPIRQLFITGLAAFPDKFWGALNTPFTDVGERKNPISLLTLIIFVSITVLVFVGARLCGQWLKKSILPQTRMERGAQAAI
STIISYTFGLIGFIILLQSVGIDLSSLAVIAGVLGLGLGFGLQELASNFVSGLTLLLEQQIRVGDFVEVDGLLGTIERIS
IRSTIVRTQDRLFVVVPNQRFFAKNVINWTYQTPESRLHIPVSVAYGTDTVLVTEALLIAARSESRVLKYPPPQVWFKSF
GDDAYKFELLAWINQPCDFEPIKSSLNFLIEQELNRKNIQIPFPQRELWLKNPEALAQALQPQPEAAAPLPTVASGEGLK
LKTGIKVTKNSTLRVLLRKVSYFENCTNAQLRVLIEQGYRQFYAPEQVIFRENESGESFYVILSGQVEVYSQKLNRQIAI
LGVGDFFGEISLLTGAPRTATMRVLEATTLFVVDRQALQKLLQNYKALAEEIAKSLSQRQQVLEELGLTQINPSESAGDD
PFIWIRRRIQTLFGI
>Mature_574_residues
TLFAVDVHFLKNLFIVVGILGGAAISDWLMGRWMQGRLRTAPKKGRAIYPLLRRCLLFGLRLGIWLVAFYNILTLIPALQ
PIRQLFITGLAAFPDKFWGALNTPFTDVGERKNPISLLTLIIFVSITVLVFVGARLCGQWLKKSILPQTRMERGAQAAIS
TIISYTFGLIGFIILLQSVGIDLSSLAVIAGVLGLGLGFGLQELASNFVSGLTLLLEQQIRVGDFVEVDGLLGTIERISI
RSTIVRTQDRLFVVVPNQRFFAKNVINWTYQTPESRLHIPVSVAYGTDTVLVTEALLIAARSESRVLKYPPPQVWFKSFG
DDAYKFELLAWINQPCDFEPIKSSLNFLIEQELNRKNIQIPFPQRELWLKNPEALAQALQPQPEAAAPLPTVASGEGLKL
KTGIKVTKNSTLRVLLRKVSYFENCTNAQLRVLIEQGYRQFYAPEQVIFRENESGESFYVILSGQVEVYSQKLNRQIAIL
GVGDFFGEISLLTGAPRTATMRVLEATTLFVVDRQALQKLLQNYKALAEEIAKSLSQRQQVLEELGLTQINPSESAGDDP
FIWIRRRIQTLFGI

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI82617546, Length=137, Percent_Identity=30.6569343065693, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786670, Length=229, Percent_Identity=37.117903930131, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI2367355, Length=254, Percent_Identity=32.2834645669291, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1789291, Length=206, Percent_Identity=30.0970873786408, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 64388; Mature: 64257

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFAVDVHFLKNLFIVVGILGGAAISDWLMGRWMQGRLRTAPKKGRAIYPLLRRCLLFG
CEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHH
LRLGIWLVAFYNILTLIPALQPIRQLFITGLAAFPDKFWGALNTPFTDVGERKNPISLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHH
LIIFVSITVLVFVGARLCGQWLKKSILPQTRMERGAQAAISTIISYTFGLIGFIILLQSV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIDLSSLAVIAGVLGLGLGFGLQELASNFVSGLTLLLEQQIRVGDFVEVDGLLGTIERIS
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
IRSTIVRTQDRLFVVVPNQRFFAKNVINWTYQTPESRLHIPVSVAYGTDTVLVTEALLIA
HHHHHHCCCCCEEEEECCCHHHHHHHHCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHH
ARSESRVLKYPPPQVWFKSFGDDAYKFELLAWINQPCDFEPIKSSLNFLIEQELNRKNIQ
CCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
IPFPQRELWLKNPEALAQALQPQPEAAAPLPTVASGEGLKLKTGIKVTKNSTLRVLLRKV
CCCCCHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEECCHHHHHHHHHH
SYFENCTNAQLRVLIEQGYRQFYAPEQVIFRENESGESFYVILSGQVEVYSQKLNRQIAI
HHHHCCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEEEECCHHHHHHHHCCEEEE
LGVGDFFGEISLLTGAPRTATMRVLEATTLFVVDRQALQKLLQNYKALAEEIAKSLSQRQ
EEECHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVLEELGLTQINPSESAGDDPFIWIRRRIQTLFGI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLFAVDVHFLKNLFIVVGILGGAAISDWLMGRWMQGRLRTAPKKGRAIYPLLRRCLLFG
EEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHH
LRLGIWLVAFYNILTLIPALQPIRQLFITGLAAFPDKFWGALNTPFTDVGERKNPISLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHH
LIIFVSITVLVFVGARLCGQWLKKSILPQTRMERGAQAAISTIISYTFGLIGFIILLQSV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIDLSSLAVIAGVLGLGLGFGLQELASNFVSGLTLLLEQQIRVGDFVEVDGLLGTIERIS
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
IRSTIVRTQDRLFVVVPNQRFFAKNVINWTYQTPESRLHIPVSVAYGTDTVLVTEALLIA
HHHHHHCCCCCEEEEECCCHHHHHHHHCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHH
ARSESRVLKYPPPQVWFKSFGDDAYKFELLAWINQPCDFEPIKSSLNFLIEQELNRKNIQ
CCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
IPFPQRELWLKNPEALAQALQPQPEAAAPLPTVASGEGLKLKTGIKVTKNSTLRVLLRKV
CCCCCHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEECCHHHHHHHHHH
SYFENCTNAQLRVLIEQGYRQFYAPEQVIFRENESGESFYVILSGQVEVYSQKLNRQIAI
HHHHCCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEEEECCHHHHHHHHCCEEEE
LGVGDFFGEISLLTGAPRTATMRVLEATTLFVVDRQALQKLLQNYKALAEEIAKSLSQRQ
EEECHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVLEELGLTQINPSESAGDDPFIWIRRRIQTLFGI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]