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The map label for this gene is cobQ

Identifier: 158337367

GI number: 158337367

Start: 4263632

End: 4265125

Strand: Reverse

Name: cobQ

Synonym: AM1_4245

Alternate gene names: 158337367

Gene position: 4265125-4263632 (Counterclockwise)

Preceding gene: 158337368

Following gene: 158337365

Centisome position: 65.58

GC content: 50.67

Gene sequence:

>1494_bases
ATGGGCGCCATTATGGTGGTCGGAACCACCTCCCATGCTGGGAAATCGATCATGGCAGCGGTGATCTGCCGAATGCTCAA
GCGCAAGGGCTATCAAGTTACCCCTTTTAAAGGGCAAAATATGGCCCTCAATGCCTACGTCACCAATGCTGGGGATGAAA
TTGGCCATGCCCAAGCTGTTCAAGCCTGGGCTGCCGGTCTCGAACCTCGGGTAGAAATGAACCCCATTTTGCTGAAACCC
CAGGGAGATATGACCTCCCAGGTAATCCTCAATGGCAAAGCCGTCGGTCGAACCCAGGCGGCCGATTACTACCGGGATTA
TTTTGATCGGGGTTGGCAGGCCATTACAACCGCATTAGTCACCCTCCAGCAAGAATTTGATTGGATTGTTTGCGAAGGAG
CCGGTAGTCCTGCTGAGATTAACCTCAAACATCGTGATCTCACGAATATGCGGGTTGCCAAACATCTTAATGCCCCCACC
TTATTAGTCGCAGATATTGATCGAGGCGGTGTGTTTGCTCATATTGTCGGCACCTTAGAACTGCTGGACCCCGACGAACG
CGCCCTAATTAAAGGATTTGTGATTAATAAATTTCGTGGACAGCGATCGCTGCTGGAATCCGGTATTACCTGGCTGGAAG
AGCGAACTGGTATTCCCGTGGTGGGCGTAATTCCCTGGCTAGAGCATTCTTTGCCAGCCGAAGACTCCTTAGATCTCTTT
GAACGACGTCGAACCAAACCCAATGCCGAGGTAACCATTGCAGTTATCCGTCTACCTCGCATCTCAAATTTCACGGATTT
TGATCCACTGGAAGCTGAACCCTCAGTGCGTTTACAGTTTGTTGGCCCAAATCAACCCTTAGGCCAGCCCGATGCCGTCA
TTGTGCCAGGGTCTAAAACCACTATTTCAGACCTACAGCAGCTCCAGGTCTCAAGTATGGCGGATCAACTTCGTGCCTAT
AGCCAAGCAGGAGGCATGGTCTTAGGGATCTGTGGGGGCTTGCAAATGTTGGGTCAAACCATCTCAGATCCAATGGGCAC
CGAAGGCCCACCCGGTGAATTTGCCGGGCTAGGGCTACTCCCCCTCCAGACCACTATGTCTGGGGACAAGATTACTCGTC
AGCGTCAAGCTCAAATCACGTTGCCATCCGACTGTTCCGCTTTAGGCGAAGATGCGAGAACGATACAAGGCTATGAAATT
CATCAAGGTCAAACCCAAGTGTTACAACCAGAAGCAGTGCAAGCATGGTTTGATGATCCAGCGTTAGGCGTAGTGAGTTG
TAACCATCGCATCTTAGGAACCTATTTACACGGAATCTTTAACAATGGGCCATGGCGACGCGTGTGGTTGAATCAGTTGC
GAGCCCAGAAAAATTTGCTTCCTCTCCCCCTTGCAATTCCAAATTATAAAGTCTATCGAGACCATATGCTGGACCAAGTC
ACCGATGCCATCGCCCCCTACCTCAATCTTCAACCTTTCCTAGGAAAGGCCTAG

Upstream 100 bases:

>100_bases
TAATTGATCAATGGCAGCATCAGGGTAAAGATATTCCAAAGCCTTCCCTTTCAGACCGATTCCAAAACCTTTGTCAGCGC
TGGGTCCGCATCCCCGTTTA

Downstream 100 bases:

>100_bases
ATCAGCCTCGTATGCTGCTGTTCTCCTCGTTCTTGACTACTGATAGGGCAAGACATGAAGGCTATCCGCTCATCCAAGAT
GGAAACCATCCAATTCACTA

Product: cobyric acid synthase CobQ

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MGAIMVVGTTSHAGKSIMAAVICRMLKRKGYQVTPFKGQNMALNAYVTNAGDEIGHAQAVQAWAAGLEPRVEMNPILLKP
QGDMTSQVILNGKAVGRTQAADYYRDYFDRGWQAITTALVTLQQEFDWIVCEGAGSPAEINLKHRDLTNMRVAKHLNAPT
LLVADIDRGGVFAHIVGTLELLDPDERALIKGFVINKFRGQRSLLESGITWLEERTGIPVVGVIPWLEHSLPAEDSLDLF
ERRRTKPNAEVTIAVIRLPRISNFTDFDPLEAEPSVRLQFVGPNQPLGQPDAVIVPGSKTTISDLQQLQVSSMADQLRAY
SQAGGMVLGICGGLQMLGQTISDPMGTEGPPGEFAGLGLLPLQTTMSGDKITRQRQAQITLPSDCSALGEDARTIQGYEI
HQGQTQVLQPEAVQAWFDDPALGVVSCNHRILGTYLHGIFNNGPWRRVWLNQLRAQKNLLPLPLAIPNYKVYRDHMLDQV
TDAIAPYLNLQPFLGKA

Sequences:

>Translated_497_residues
MGAIMVVGTTSHAGKSIMAAVICRMLKRKGYQVTPFKGQNMALNAYVTNAGDEIGHAQAVQAWAAGLEPRVEMNPILLKP
QGDMTSQVILNGKAVGRTQAADYYRDYFDRGWQAITTALVTLQQEFDWIVCEGAGSPAEINLKHRDLTNMRVAKHLNAPT
LLVADIDRGGVFAHIVGTLELLDPDERALIKGFVINKFRGQRSLLESGITWLEERTGIPVVGVIPWLEHSLPAEDSLDLF
ERRRTKPNAEVTIAVIRLPRISNFTDFDPLEAEPSVRLQFVGPNQPLGQPDAVIVPGSKTTISDLQQLQVSSMADQLRAY
SQAGGMVLGICGGLQMLGQTISDPMGTEGPPGEFAGLGLLPLQTTMSGDKITRQRQAQITLPSDCSALGEDARTIQGYEI
HQGQTQVLQPEAVQAWFDDPALGVVSCNHRILGTYLHGIFNNGPWRRVWLNQLRAQKNLLPLPLAIPNYKVYRDHMLDQV
TDAIAPYLNLQPFLGKA
>Mature_496_residues
GAIMVVGTTSHAGKSIMAAVICRMLKRKGYQVTPFKGQNMALNAYVTNAGDEIGHAQAVQAWAAGLEPRVEMNPILLKPQ
GDMTSQVILNGKAVGRTQAADYYRDYFDRGWQAITTALVTLQQEFDWIVCEGAGSPAEINLKHRDLTNMRVAKHLNAPTL
LVADIDRGGVFAHIVGTLELLDPDERALIKGFVINKFRGQRSLLESGITWLEERTGIPVVGVIPWLEHSLPAEDSLDLFE
RRRTKPNAEVTIAVIRLPRISNFTDFDPLEAEPSVRLQFVGPNQPLGQPDAVIVPGSKTTISDLQQLQVSSMADQLRAYS
QAGGMVLGICGGLQMLGQTISDPMGTEGPPGEFAGLGLLPLQTTMSGDKITRQRQAQITLPSDCSALGEDARTIQGYEIH
QGQTQVLQPEAVQAWFDDPALGVVSCNHRILGTYLHGIFNNGPWRRVWLNQLRAQKNLLPLPLAIPNYKVYRDHMLDQVT
DAIAPYLNLQPFLGKA

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_ACAM1 (B0CCR3)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518542.1
- ProteinModelPortal:   B0CCR3
- GeneID:   5683048
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4245
- HOGENOM:   HBG302271
- OMA:   DAISGTR
- ProtClustDB:   CLSK2475851
- BioCyc:   AMAR329726:AM1_4245-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 54480; Mature: 54349

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 331-331 ACT_SITE 437-437

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAIMVVGTTSHAGKSIMAAVICRMLKRKGYQVTPFKGQNMALNAYVTNAGDEIGHAQAV
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEEEECCCCHHHHHHHH
QAWAAGLEPRVEMNPILLKPQGDMTSQVILNGKAVGRTQAADYYRDYFDRGWQAITTALV
HHHHHCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLQQEFDWIVCEGAGSPAEINLKHRDLTNMRVAKHLNAPTLLVADIDRGGVFAHIVGTLE
HHHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEHE
LLDPDERALIKGFVINKFRGQRSLLESGITWLEERTGIPVVGVIPWLEHSLPAEDSLDLF
ECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCHHHH
ERRRTKPNAEVTIAVIRLPRISNFTDFDPLEAEPSVRLQFVGPNQPLGQPDAVIVPGSKT
HHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCC
TISDLQQLQVSSMADQLRAYSQAGGMVLGICGGLQMLGQTISDPMGTEGPPGEFAGLGLL
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
PLQTTMSGDKITRQRQAQITLPSDCSALGEDARTIQGYEIHQGQTQVLQPEAVQAWFDDP
EECCCCCCCHHHHHHHCEEECCCCHHHHCCCHHCCCCEEECCCCCCCCCHHHHHHHCCCC
ALGVVSCNHRILGTYLHGIFNNGPWRRVWLNQLRAQKNLLPLPLAIPNYKVYRDHMLDQV
CCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
TDAIAPYLNLQPFLGKA
HHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
GAIMVVGTTSHAGKSIMAAVICRMLKRKGYQVTPFKGQNMALNAYVTNAGDEIGHAQAV
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEEEECCCCHHHHHHHH
QAWAAGLEPRVEMNPILLKPQGDMTSQVILNGKAVGRTQAADYYRDYFDRGWQAITTALV
HHHHHCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLQQEFDWIVCEGAGSPAEINLKHRDLTNMRVAKHLNAPTLLVADIDRGGVFAHIVGTLE
HHHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEHE
LLDPDERALIKGFVINKFRGQRSLLESGITWLEERTGIPVVGVIPWLEHSLPAEDSLDLF
ECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCHHHH
ERRRTKPNAEVTIAVIRLPRISNFTDFDPLEAEPSVRLQFVGPNQPLGQPDAVIVPGSKT
HHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCC
TISDLQQLQVSSMADQLRAYSQAGGMVLGICGGLQMLGQTISDPMGTEGPPGEFAGLGLL
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
PLQTTMSGDKITRQRQAQITLPSDCSALGEDARTIQGYEIHQGQTQVLQPEAVQAWFDDP
EECCCCCCCHHHHHHHCEEECCCCHHHHCCCHHCCCCEEECCCCCCCCCHHHHHHHCCCC
ALGVVSCNHRILGTYLHGIFNNGPWRRVWLNQLRAQKNLLPLPLAIPNYKVYRDHMLDQV
CCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
TDAIAPYLNLQPFLGKA
HHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA