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The map label for this gene is yfjR [H]

Identifier: 158337293

GI number: 158337293

Start: 4201008

End: 4201880

Strand: Reverse

Name: yfjR [H]

Synonym: AM1_4171

Alternate gene names: 158337293

Gene position: 4201880-4201008 (Counterclockwise)

Preceding gene: 158337294

Following gene: 158337291

Centisome position: 64.61

GC content: 52.12

Gene sequence:

>873_bases
ATGAACAAGATTGCGATTTTAGGGTTAGGCGCAATGGGTTCCCGCGTGGCCATCAATTTGCTCGCAGCAGGCTATGAAGT
GATTGTCTGGAACCGATCTCCCCAAGCTGTGCACACCCTCGTTCAAGCAGGGGCAATGGCAGCGGATTCCCCGCAAGAAG
CGGCGGTCCAAGCCTCGATTGTGATCAGTATGGTAACGGATGACCCAGCATCCCAAGCAGTTTGGTTAGATCCTGAAACT
GGGGCAGCCCAGGGGTTAGCCCCAGAGGCCATTGCCATTGAATCTAGCTCCCTAACGGTGGGCCATACCCAAACCTTAGC
AACAGAAATTGAACAGCGTGGGGCTGCCTTTCTCGCAGCTCCCGTGGTCGGCTCCCGACCCCAAGCCGAAGCTCAACAAC
TGATTTACTTAGTTGGCGGAAAAGCCCCAGTTCTGGCCAAGGTTCAAGATGTTTTATCAGCCTCCAGCTCCAGAATTCAT
CATGTGGGAACCGTGGGGCAGAGTATGGCGATGAAGCTGGCGGTCAATGCCTTATTTGGGGTGCAAGTGGCTGCTGTCGC
CGAAATCATCAGCCTGTTAAATCAGCAGGGTATACCTCTCAACCAGGTTATGGCGACACTAAAGGAACTGCCAGTGACCA
GCCCAGCGGTGCAAGGGGCCGGTAATCTGATGATTACCCATGCCCATGCCCCTTTATTCCCAATTTCTTTAGTCGAGAAA
GATATTCGATATGGACTGGAGCTAGCAAAAAATGCTGTCGAGGAGCCGATGCCCACTTTGAAGGCAGTTCAAAATGTGTA
TCAGGATGCGATCGCACAAGGATATGGCAATGACAACATTACGGGTGTCATTCAACTGTTTATGCAGCCTTAG

Upstream 100 bases:

>100_bases
ATTCATAAAGAGCTTTGGGTTCTCCAGGCCGATGGTACCGCTAAGCTCCGAGTAGATGACTTCGAGGCGATGAGCTAGAG
ACGTTGAGGAAAAATCAAAT

Downstream 100 bases:

>100_bases
CCCTTGCATCATAAGCTAGCCAGCAAGTCCCCCTTAATATCAAGATTTTGGGCACTAATCCACTTGGAACGAGGGAGTGA
CTTGAGAAGGCTGTAGGGTT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNKIAILGLGAMGSRVAINLLAAGYEVIVWNRSPQAVHTLVQAGAMAADSPQEAAVQASIVISMVTDDPASQAVWLDPET
GAAQGLAPEAIAIESSSLTVGHTQTLATEIEQRGAAFLAAPVVGSRPQAEAQQLIYLVGGKAPVLAKVQDVLSASSSRIH
HVGTVGQSMAMKLAVNALFGVQVAAVAEIISLLNQQGIPLNQVMATLKELPVTSPAVQGAGNLMITHAHAPLFPISLVEK
DIRYGLELAKNAVEEPMPTLKAVQNVYQDAIAQGYGNDNITGVIQLFMQP

Sequences:

>Translated_290_residues
MNKIAILGLGAMGSRVAINLLAAGYEVIVWNRSPQAVHTLVQAGAMAADSPQEAAVQASIVISMVTDDPASQAVWLDPET
GAAQGLAPEAIAIESSSLTVGHTQTLATEIEQRGAAFLAAPVVGSRPQAEAQQLIYLVGGKAPVLAKVQDVLSASSSRIH
HVGTVGQSMAMKLAVNALFGVQVAAVAEIISLLNQQGIPLNQVMATLKELPVTSPAVQGAGNLMITHAHAPLFPISLVEK
DIRYGLELAKNAVEEPMPTLKAVQNVYQDAIAQGYGNDNITGVIQLFMQP
>Mature_290_residues
MNKIAILGLGAMGSRVAINLLAAGYEVIVWNRSPQAVHTLVQAGAMAADSPQEAAVQASIVISMVTDDPASQAVWLDPET
GAAQGLAPEAIAIESSSLTVGHTQTLATEIEQRGAAFLAAPVVGSRPQAEAQQLIYLVGGKAPVLAKVQDVLSASSSRIH
HVGTVGQSMAMKLAVNALFGVQVAAVAEIISLLNQQGIPLNQVMATLKELPVTSPAVQGAGNLMITHAHAPLFPISLVEK
DIRYGLELAKNAVEEPMPTLKAVQNVYQDAIAQGYGNDNITGVIQLFMQP

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI23308751, Length=300, Percent_Identity=25.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI145693186, Length=274, Percent_Identity=28.1021897810219, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=29.1095890410959, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786719, Length=272, Percent_Identity=26.8382352941176, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=25.8741258741259, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17557316, Length=283, Percent_Identity=25.7950530035336, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=26.1484098939929, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24655230, Length=297, Percent_Identity=28.956228956229, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922568, Length=297, Percent_Identity=28.956228956229, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30189; Mature: 30189

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIAILGLGAMGSRVAINLLAAGYEVIVWNRSPQAVHTLVQAGAMAADSPQEAAVQASI
CCCEEEEEECCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHEEEE
VISMVTDDPASQAVWLDPETGAAQGLAPEAIAIESSSLTVGHTQTLATEIEQRGAAFLAA
EEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEECCCEEECCHHHHHHHHHHCCCEEEEE
PVVGSRPQAEAQQLIYLVGGKAPVLAKVQDVLSASSSRIHHVGTVGQSMAMKLAVNALFG
CCCCCCCHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
VQVAAVAEIISLLNQQGIPLNQVMATLKELPVTSPAVQGAGNLMITHAHAPLFPISLVEK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCHHHHHHH
DIRYGLELAKNAVEEPMPTLKAVQNVYQDAIAQGYGNDNITGVIQLFMQP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEECC
>Mature Secondary Structure
MNKIAILGLGAMGSRVAINLLAAGYEVIVWNRSPQAVHTLVQAGAMAADSPQEAAVQASI
CCCEEEEEECCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHEEEE
VISMVTDDPASQAVWLDPETGAAQGLAPEAIAIESSSLTVGHTQTLATEIEQRGAAFLAA
EEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEECCCEEECCHHHHHHHHHHCCCEEEEE
PVVGSRPQAEAQQLIYLVGGKAPVLAKVQDVLSASSSRIHHVGTVGQSMAMKLAVNALFG
CCCCCCCHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
VQVAAVAEIISLLNQQGIPLNQVMATLKELPVTSPAVQGAGNLMITHAHAPLFPISLVEK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCHHHHHHH
DIRYGLELAKNAVEEPMPTLKAVQNVYQDAIAQGYGNDNITGVIQLFMQP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]