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The map label for this gene is nth [H]

Identifier: 158337187

GI number: 158337187

Start: 4112073

End: 4112747

Strand: Direct

Name: nth [H]

Synonym: AM1_4064

Alternate gene names: 158337187

Gene position: 4112073-4112747 (Clockwise)

Preceding gene: 158337186

Following gene: 158337188

Centisome position: 63.23

GC content: 50.96

Gene sequence:

>675_bases
ATGCCCCGACCTGCTGCCAAAAAAGTAAAGGCATTAGAATTACTCGATCGCCTTAAACAGCTCTATCCAGAGGCAACCTG
TAGCTTGACCTATGAAACACCGGTTCAGCTCCTGGTGGCGACAATTCTGTCGGCTCAGTGTACGGATGAGCGGGTGAATC
AAGTGACCCCAGCCCTGTTTGAAGCCTATCCAGATGCCCTTGCCTTCGCCGAAGCGGATCGGGATGCACTAGAGACGCTC
ATTCGCTCAACAGGGTTTTATCGCAACAAAGCCAAGCATATTCAAGGGGCTTGCCAAAAGATTGTCAGGGACTTTGACGG
CCAGGTTCCGAATCAAATGGATCTTTTGTTAACCCTACCTGGAGTGGCCCGCAAGACCGCTAATGTTGTCCTAGCCCATG
GTTATGGCATTAATATGGGGGTCACCGTAGATACTCATGTCAAGCGCTTAAGCAATCGGTTGGGGCTGACTCGTCATCAA
GATCCCGTCAAAATTGAGCAGGATTTGATGAAGCTGTTACCCCAGGAAGACTGGGAAAACTGGTCGATTCGCCTGATTTA
TCATGGCCGAGCTGTTTGTAGTGCTCGGAAACCCACTTGCGATCGCTGTTCTTTGACGGATCTCTGTCCGTCAGCGGGTA
AGGTTACCCCAGCTCGCTCTGCCAAAAGTCAGTGA

Upstream 100 bases:

>100_bases
CAAGATTCAAGAAGGTGTCATGCAGGTTAGCCTCTTTCTCTTGTTGGGGCTGGGGGTATTTATGATTGTTCTGGATACCG
CCAAACTGATGACTTAGGCT

Downstream 100 bases:

>100_bases
AATCTTTCGAAAAAAAGGACAAGATTCGTTAAGATAGAAAATACTGTTTAAATTTTTGATTGGACATCCCACAACTTCAT
GGCTAAAAAAGGCATGGTTG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPRPAAKKVKALELLDRLKQLYPEATCSLTYETPVQLLVATILSAQCTDERVNQVTPALFEAYPDALAFAEADRDALETL
IRSTGFYRNKAKHIQGACQKIVRDFDGQVPNQMDLLLTLPGVARKTANVVLAHGYGINMGVTVDTHVKRLSNRLGLTRHQ
DPVKIEQDLMKLLPQEDWENWSIRLIYHGRAVCSARKPTCDRCSLTDLCPSAGKVTPARSAKSQ

Sequences:

>Translated_224_residues
MPRPAAKKVKALELLDRLKQLYPEATCSLTYETPVQLLVATILSAQCTDERVNQVTPALFEAYPDALAFAEADRDALETL
IRSTGFYRNKAKHIQGACQKIVRDFDGQVPNQMDLLLTLPGVARKTANVVLAHGYGINMGVTVDTHVKRLSNRLGLTRHQ
DPVKIEQDLMKLLPQEDWENWSIRLIYHGRAVCSARKPTCDRCSLTDLCPSAGKVTPARSAKSQ
>Mature_223_residues
PRPAAKKVKALELLDRLKQLYPEATCSLTYETPVQLLVATILSAQCTDERVNQVTPALFEAYPDALAFAEADRDALETLI
RSTGFYRNKAKHIQGACQKIVRDFDGQVPNQMDLLLTLPGVARKTANVVLAHGYGINMGVTVDTHVKRLSNRLGLTRHQD
PVKIEQDLMKLLPQEDWENWSIRLIYHGRAVCSARKPTCDRCSLTDLCPSAGKVTPARSAKSQ

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=30.7262569832402, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1787920, Length=211, Percent_Identity=38.8625592417062, Blast_Score=147, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17554540, Length=190, Percent_Identity=35.2631578947368, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=33.1578947368421, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=28.735632183908, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=191, Percent_Identity=31.413612565445, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24926; Mature: 24795

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPAAKKVKALELLDRLKQLYPEATCSLTYETPVQLLVATILSAQCTDERVNQVTPALF
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EAYPDALAFAEADRDALETLIRSTGFYRNKAKHIQGACQKIVRDFDGQVPNQMDLLLTLP
HHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECC
GVARKTANVVLAHGYGINMGVTVDTHVKRLSNRLGLTRHQDPVKIEQDLMKLLPQEDWEN
CHHHHHHCEEEECCCCCEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
WSIRLIYHGRAVCSARKPTCDRCSLTDLCPSAGKVTPARSAKSQ
CEEEEEEECCHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRPAAKKVKALELLDRLKQLYPEATCSLTYETPVQLLVATILSAQCTDERVNQVTPALF
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EAYPDALAFAEADRDALETLIRSTGFYRNKAKHIQGACQKIVRDFDGQVPNQMDLLLTLP
HHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECC
GVARKTANVVLAHGYGINMGVTVDTHVKRLSNRLGLTRHQDPVKIEQDLMKLLPQEDWEN
CHHHHHHCEEEECCCCCEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
WSIRLIYHGRAVCSARKPTCDRCSLTDLCPSAGKVTPARSAKSQ
CEEEEEEECCHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]