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The map label for this gene is ubiE [H]

Identifier: 158337171

GI number: 158337171

Start: 4091666

End: 4092298

Strand: Reverse

Name: ubiE [H]

Synonym: AM1_4047

Alternate gene names: 158337171

Gene position: 4092298-4091666 (Counterclockwise)

Preceding gene: 158337173

Following gene: 158337169

Centisome position: 62.92

GC content: 45.66

Gene sequence:

>633_bases
ATGACTGCGAATGTTCTAGCTGCCAAAACCCGAGTATTCGATCAGTGGGCTCCCAATTACGATTGGTTGTTCACAACAGT
GTTTTATCAAGCCGTTCATCAACGTCTGTTGAGCTATGTCAATTTGCCTAAGGATTCACCGGCTGCTGTCCTAGATATCG
GTTGTGGTACAGGTAAGCTCCTCAACCGCTTAGCTTCTCAATTCCCTCAATTGCAGGGAACAGGACTGGATCTATCCCCA
GAAATGCTACGGCAGGCTCGTCAACGGAACAGTCACCGCCCTCGCTTAATTTTTGTGCAAGGGGCTAGTGAAGCCATGCC
TTTTGCAGATAATCAATTCCAGGCTGCTTTTAGTACCATCAGCTTCTTACATTATCCGGATCCACAACAGGTATTCTTAG
AAATTGGGCGTGTCCTCAAACCCGGTGGTCAGTTTCATTTAGTCGATTACACAACCTTCTTAGAATTAGGCACTCAGCAC
AATATTGCGGGTTTGGGTGGTCAGATGTATTTTTATAGTCCGCAGCAGCGCAAGACGCTAGCAGCAAATGCTGGCCTGAT
CTGTAATGCTCATCATTCGTTGTTAGGGCCTGTATTACTCTCCATATTTCAAAAACCCAATCTGTCTTATTAA

Upstream 100 bases:

>100_bases
CTTCCCCAAACTGTCAACCTTTATCTCCGACTTTCCCAAAAAAATACCTGTGCATCGATTGTCCTTGCTAGTATGACCAT
AGATCAGGCCTAATCCCACA

Downstream 100 bases:

>100_bases
GATTATTCAAGTCATTCAATTTCTGCATCGATAAGTCATTTTCACATCATGATCTCAAAACGACTCACTTTGGGGTAGAG
ACACACGCTACTATCAGTTG

Product: UbiE/COQ5 family methlytransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTANVLAAKTRVFDQWAPNYDWLFTTVFYQAVHQRLLSYVNLPKDSPAAVLDIGCGTGKLLNRLASQFPQLQGTGLDLSP
EMLRQARQRNSHRPRLIFVQGASEAMPFADNQFQAAFSTISFLHYPDPQQVFLEIGRVLKPGGQFHLVDYTTFLELGTQH
NIAGLGGQMYFYSPQQRKTLAANAGLICNAHHSLLGPVLLSIFQKPNLSY

Sequences:

>Translated_210_residues
MTANVLAAKTRVFDQWAPNYDWLFTTVFYQAVHQRLLSYVNLPKDSPAAVLDIGCGTGKLLNRLASQFPQLQGTGLDLSP
EMLRQARQRNSHRPRLIFVQGASEAMPFADNQFQAAFSTISFLHYPDPQQVFLEIGRVLKPGGQFHLVDYTTFLELGTQH
NIAGLGGQMYFYSPQQRKTLAANAGLICNAHHSLLGPVLLSIFQKPNLSY
>Mature_209_residues
TANVLAAKTRVFDQWAPNYDWLFTTVFYQAVHQRLLSYVNLPKDSPAAVLDIGCGTGKLLNRLASQFPQLQGTGLDLSPE
MLRQARQRNSHRPRLIFVQGASEAMPFADNQFQAAFSTISFLHYPDPQQVFLEIGRVLKPGGQFHLVDYTTFLELGTQHN
IAGLGGQMYFYSPQQRKTLAANAGLICNAHHSLLGPVLLSIFQKPNLSY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 23468; Mature: 23337

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANVLAAKTRVFDQWAPNYDWLFTTVFYQAVHQRLLSYVNLPKDSPAAVLDIGCGTGKL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
LNRLASQFPQLQGTGLDLSPEMLRQARQRNSHRPRLIFVQGASEAMPFADNQFQAAFSTI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
SFLHYPDPQQVFLEIGRVLKPGGQFHLVDYTTFLELGTQHNIAGLGGQMYFYSPQQRKTL
HHHCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCCEEEEECCHHHHHH
AANAGLICNAHHSLLGPVLLSIFQKPNLSY
HCCCCEEEECHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TANVLAAKTRVFDQWAPNYDWLFTTVFYQAVHQRLLSYVNLPKDSPAAVLDIGCGTGKL
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
LNRLASQFPQLQGTGLDLSPEMLRQARQRNSHRPRLIFVQGASEAMPFADNQFQAAFSTI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
SFLHYPDPQQVFLEIGRVLKPGGQFHLVDYTTFLELGTQHNIAGLGGQMYFYSPQQRKTL
HHHCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCCEEEEECCHHHHHH
AANAGLICNAHHSLLGPVLLSIFQKPNLSY
HCCCCEEEECHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]