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The map label for this gene is gltA [H]

Identifier: 158337128

GI number: 158337128

Start: 4049124

End: 4050317

Strand: Reverse

Name: gltA [H]

Synonym: AM1_4002

Alternate gene names: 158337128

Gene position: 4050317-4049124 (Counterclockwise)

Preceding gene: 158337134

Following gene: 158337127

Centisome position: 62.28

GC content: 50.0

Gene sequence:

>1194_bases
ATGAGAGAACGATTTTATCGACAGCAGGACGATACTATTCCCACCGGAGCAAAGCAGATGACAACTTGTGATTATGTGCC
TGGCTTAGGTGGAATTCCAGCCGCCCAGTCCCGAGTCAGCTATGTGGATGGTCAGGCGGGGCAACTGGAATATCGCGGCA
TTCCCATTACCGAGCTAGCAGAACACAGCAATTTTCTGGAAACCGCATTCTTGTTAATTTGGGGCCATCTGCCCACTCCT
GAAGATTTGAGCCTGTTTCAGTACGAAGTCATCCACCATCGCCGCATTAAATACCGTCTAGTGGATATGATGAAATGCTT
CCCTGAGCGAGGTCATCCCCTAGACGCAATACAAGCTACGGTAGCAGCGTTAGGGCTGTACCATTCCCGTCGCGCCTTTA
ACGATCCAGCCTATATTCGCAGAGCGGTGGTGCGGCTCTTAGCCAAAATTCCCACTGCCGTCGCGGCCTTTGAACATATT
CGTAAGGGTAACGATCCTCTCCGTCCCCGGGATGATCTGAATTACGCCGCTAATTTCTTATATCTTCTCAGCGAGCAAGA
ACCGGATCCGCAAAAAGCCCATATTTTCGATCAGTGTTTGATTCTGCATGCCGAACACACCATGAATGCCTCGACCTTTT
CGGCTAGAGTCACGGCATCAACCCTAACAGACCCCTATGCAGTAGTCGCCTCAGCCATGGGTACTTTAGGTGGCCGACTC
CATGGGGGTGCGAACGAAGAAGTGATGCAAATGCTCACGGATATTGGCTCCGTTGAGAATGTGAAGCCCTATTTGGATCA
GCAATTGGCCCAAGGTCGTCGAATCATGGGGTTTGGCCATCGAATTTATAAGGTTAAAGATCCTCGCGCCCAGATTATGC
AGGGGTTAGCTGAACAGTTGTCTGAAAACTATGGATGCGATCGCTACTACGGTATTGCCCTCGCCTTAGAAAAAGCCGTC
GAAGAACGGCTTGGCGATCGTCAAATTCATGCCAATGTGGAATTCTATTCTGGGTTAATCTTTAGACAACTAGGGTTTCC
CGCCGATATTTTTACCTCTCTTTTTGCCATTGCTCGGGTCGCCGGATGGCTGGCCCACTGGAAAGAACAGCTCGATACCA
ACCGGATCTACCGTCCTTCCCAAATCTATATGGGACAACATGATCAGCCCTATATTCCTGTGGTGGATCGGTAG

Upstream 100 bases:

>100_bases
CCTGATCGCCATCATTAGTCAAAGTTGTGCCGTGTTTTCTCCTATCTGGATAACGGCCAGATCCTCTAATTGACAGGTAC
AAACAGAGCCTGTCCAATGG

Downstream 100 bases:

>100_bases
CTCCTTATTATCAGGAGAGAACGATGAGCCCAAAAACGCAACGACGCACCTTTCTACTGAGTAGTCTCGCAGCGACAGGG
GGTCTACTGGGCAGCATATT

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRERFYRQQDDTIPTGAKQMTTCDYVPGLGGIPAAQSRVSYVDGQAGQLEYRGIPITELAEHSNFLETAFLLIWGHLPTP
EDLSLFQYEVIHHRRIKYRLVDMMKCFPERGHPLDAIQATVAALGLYHSRRAFNDPAYIRRAVVRLLAKIPTAVAAFEHI
RKGNDPLRPRDDLNYAANFLYLLSEQEPDPQKAHIFDQCLILHAEHTMNASTFSARVTASTLTDPYAVVASAMGTLGGRL
HGGANEEVMQMLTDIGSVENVKPYLDQQLAQGRRIMGFGHRIYKVKDPRAQIMQGLAEQLSENYGCDRYYGIALALEKAV
EERLGDRQIHANVEFYSGLIFRQLGFPADIFTSLFAIARVAGWLAHWKEQLDTNRIYRPSQIYMGQHDQPYIPVVDR

Sequences:

>Translated_397_residues
MRERFYRQQDDTIPTGAKQMTTCDYVPGLGGIPAAQSRVSYVDGQAGQLEYRGIPITELAEHSNFLETAFLLIWGHLPTP
EDLSLFQYEVIHHRRIKYRLVDMMKCFPERGHPLDAIQATVAALGLYHSRRAFNDPAYIRRAVVRLLAKIPTAVAAFEHI
RKGNDPLRPRDDLNYAANFLYLLSEQEPDPQKAHIFDQCLILHAEHTMNASTFSARVTASTLTDPYAVVASAMGTLGGRL
HGGANEEVMQMLTDIGSVENVKPYLDQQLAQGRRIMGFGHRIYKVKDPRAQIMQGLAEQLSENYGCDRYYGIALALEKAV
EERLGDRQIHANVEFYSGLIFRQLGFPADIFTSLFAIARVAGWLAHWKEQLDTNRIYRPSQIYMGQHDQPYIPVVDR
>Mature_397_residues
MRERFYRQQDDTIPTGAKQMTTCDYVPGLGGIPAAQSRVSYVDGQAGQLEYRGIPITELAEHSNFLETAFLLIWGHLPTP
EDLSLFQYEVIHHRRIKYRLVDMMKCFPERGHPLDAIQATVAALGLYHSRRAFNDPAYIRRAVVRLLAKIPTAVAAFEHI
RKGNDPLRPRDDLNYAANFLYLLSEQEPDPQKAHIFDQCLILHAEHTMNASTFSARVTASTLTDPYAVVASAMGTLGGRL
HGGANEEVMQMLTDIGSVENVKPYLDQQLAQGRRIMGFGHRIYKVKDPRAQIMQGLAEQLSENYGCDRYYGIALALEKAV
EERLGDRQIHANVEFYSGLIFRQLGFPADIFTSLFAIARVAGWLAHWKEQLDTNRIYRPSQIYMGQHDQPYIPVVDR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=409, Percent_Identity=27.1393643031785, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786939, Length=394, Percent_Identity=34.2639593908629, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1786527, Length=390, Percent_Identity=31.5384615384615, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17555174, Length=324, Percent_Identity=27.4691358024691, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319850, Length=379, Percent_Identity=24.802110817942, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=368, Percent_Identity=24.7282608695652, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=402, Percent_Identity=23.3830845771144, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640124, Length=399, Percent_Identity=25.8145363408521, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24640126, Length=401, Percent_Identity=26.1845386533666, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356863, Length=389, Percent_Identity=25.1928020565553, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 44995; Mature: 44995

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERFYRQQDDTIPTGAKQMTTCDYVPGLGGIPAAQSRVSYVDGQAGQLEYRGIPITELA
CCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCHHHHH
EHSNFLETAFLLIWGHLPTPEDLSLFQYEVIHHRRIKYRLVDMMKCFPERGHPLDAIQAT
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VAALGLYHSRRAFNDPAYIRRAVVRLLAKIPTAVAAFEHIRKGNDPLRPRDDLNYAANFL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YLLSEQEPDPQKAHIFDQCLILHAEHTMNASTFSARVTASTLTDPYAVVASAMGTLGGRL
HEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHHCCCC
HGGANEEVMQMLTDIGSVENVKPYLDQQLAQGRRIMGFGHRIYKVKDPRAQIMQGLAEQL
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCEEEEECCCHHHHHHHHHHHH
SENYGCDRYYGIALALEKAVEERLGDRQIHANVEFYSGLIFRQLGFPADIFTSLFAIARV
HHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AGWLAHWKEQLDTNRIYRPSQIYMGQHDQPYIPVVDR
HHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]