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The map label for this gene is fcl [C]

Identifier: 158337095

GI number: 158337095

Start: 4017989

End: 4018933

Strand: Reverse

Name: fcl [C]

Synonym: AM1_3968

Alternate gene names: 158337095

Gene position: 4018933-4017989 (Counterclockwise)

Preceding gene: 158337097

Following gene: 158337094

Centisome position: 61.79

GC content: 47.2

Gene sequence:

>945_bases
ATGACTACATCCAATCTTGCCAGCAAAAAAGTTCTAGTCACCGGAGGCGCAGGTTTTCTGGGCCGTCAAGTCATTGCTCA
ACTACAAAAAGCCGGAGCACAACTTGAGAATATTTCCATCCCTCGCTCTTCTACCTGCGATTTGCGATCCCTAAGTGCTT
GTCAAGACGCTGTTGCCGGACAAGATATTGTCATCCACTTAGCAGCCCATGTTGGCGGCATTGGTTTAAATCAAATTAAA
CCTGCCGAATTGTTCTACGACAATTTGATGATGGGCACCCAACTCATTCATGCGGCCTATGAAAAAGGCGTCGAAAAATT
TGTCTGTGTGGGTACCATTTGTGCCTATCCCAAATTTACGCCCGTCCCCTTCAAAGAAGAAGCTCTCTGGGATGGATATC
CCGAAGAAACCAATGCTCCCTATGGGGTGGCCAAAAAAGCATTACTCGTCCAGCTCCAAGCCTATCGGCAGCAGTATGGA
TTTAACGGGGTATATCTACTCCCTGTGAATTTATATGGTCCAGAAGATAATTTTAATCCACAAAGCTCCCATGTCATCCC
TGCTCTGATTCGCAAAGTCTATGAAGCCCAGCAAGCTGGGGCGACCCAACTCGAAGTATGGGGGGATGGCACACCGACCC
GGGAGTTTTTGTACTCTGAGGATGCTGCACGGGGAATAATAATGGCGATGGAAGATTACAACGAATCGGACCCCATTAAC
TTAGGAACGAACTCTGAAGTCTCCATTCGTGATCTCGTCACCTTAATTTGTCGATTGATGGACTTTAAAGGTGACGTGGT
TTGGCTCACGGATAAGCCAAATGGTCAGCCACGTCGTTGTTTGGATACGGCTAAAGCTAAAGAAAAATTCGGCTTTACCG
CCAACATCACCTTAGAGCAGGGATTACAGAACACCATTGATTGGTACCGACAGCACCCCGAGTAA

Upstream 100 bases:

>100_bases
TTTAAAAAATAGATGAGAATGTCTAAGCTAGCATTATTAGCCTAGCATTCCCAGCTTAATTCGGTTGTCCGTCAATAACT
GTATTTGACAAAACATAATC

Downstream 100 bases:

>100_bases
CTGTAAGCACGAGAGTCATGGCAACGAGGCCACCCATGACTCTCGCAAAACCATATCCATGCCGGTGCCTCAAGAGTCCC
CTGCTGTCTTTAATCCCCAA

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTTSNLASKKVLVTGGAGFLGRQVIAQLQKAGAQLENISIPRSSTCDLRSLSACQDAVAGQDIVIHLAAHVGGIGLNQIK
PAELFYDNLMMGTQLIHAAYEKGVEKFVCVGTICAYPKFTPVPFKEEALWDGYPEETNAPYGVAKKALLVQLQAYRQQYG
FNGVYLLPVNLYGPEDNFNPQSSHVIPALIRKVYEAQQAGATQLEVWGDGTPTREFLYSEDAARGIIMAMEDYNESDPIN
LGTNSEVSIRDLVTLICRLMDFKGDVVWLTDKPNGQPRRCLDTAKAKEKFGFTANITLEQGLQNTIDWYRQHPE

Sequences:

>Translated_314_residues
MTTSNLASKKVLVTGGAGFLGRQVIAQLQKAGAQLENISIPRSSTCDLRSLSACQDAVAGQDIVIHLAAHVGGIGLNQIK
PAELFYDNLMMGTQLIHAAYEKGVEKFVCVGTICAYPKFTPVPFKEEALWDGYPEETNAPYGVAKKALLVQLQAYRQQYG
FNGVYLLPVNLYGPEDNFNPQSSHVIPALIRKVYEAQQAGATQLEVWGDGTPTREFLYSEDAARGIIMAMEDYNESDPIN
LGTNSEVSIRDLVTLICRLMDFKGDVVWLTDKPNGQPRRCLDTAKAKEKFGFTANITLEQGLQNTIDWYRQHPE
>Mature_313_residues
TTSNLASKKVLVTGGAGFLGRQVIAQLQKAGAQLENISIPRSSTCDLRSLSACQDAVAGQDIVIHLAAHVGGIGLNQIKP
AELFYDNLMMGTQLIHAAYEKGVEKFVCVGTICAYPKFTPVPFKEEALWDGYPEETNAPYGVAKKALLVQLQAYRQQYGF
NGVYLLPVNLYGPEDNFNPQSSHVIPALIRKVYEAQQAGATQLEVWGDGTPTREFLYSEDAARGIIMAMEDYNESDPINL
GTNSEVSIRDLVTLICRLMDFKGDVVWLTDKPNGQPRRCLDTAKAKEKFGFTANITLEQGLQNTIDWYRQHPE

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=317, Percent_Identity=34.384858044164, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI7657641, Length=334, Percent_Identity=26.3473053892216, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=24.2990654205607, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1788365, Length=315, Percent_Identity=37.7777777777778, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788353, Length=345, Percent_Identity=24.9275362318841, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=23.121387283237, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786974, Length=341, Percent_Identity=23.7536656891496, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554428, Length=308, Percent_Identity=35.3896103896104, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17568069, Length=261, Percent_Identity=25.6704980842912, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922778, Length=311, Percent_Identity=32.1543408360129, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21356223, Length=316, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34653; Mature: 34522

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSNLASKKVLVTGGAGFLGRQVIAQLQKAGAQLENISIPRSSTCDLRSLSACQDAVAG
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHCC
QDIVIHLAAHVGGIGLNQIKPAELFYDNLMMGTQLIHAAYEKGVEKFVCVGTICAYPKFT
CHHEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVPFKEEALWDGYPEETNAPYGVAKKALLVQLQAYRQQYGFNGVYLLPVNLYGPEDNFNP
CCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC
QSSHVIPALIRKVYEAQQAGATQLEVWGDGTPTREFLYSEDAARGIIMAMEDYNESDPIN
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEEECCCCCCCEE
LGTNSEVSIRDLVTLICRLMDFKGDVVWLTDKPNGQPRRCLDTAKAKEKFGFTANITLEQ
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHH
GLQNTIDWYRQHPE
HHHHHHHHHHCCCC
>Mature Secondary Structure 
TTSNLASKKVLVTGGAGFLGRQVIAQLQKAGAQLENISIPRSSTCDLRSLSACQDAVAG
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHCC
QDIVIHLAAHVGGIGLNQIKPAELFYDNLMMGTQLIHAAYEKGVEKFVCVGTICAYPKFT
CHHEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVPFKEEALWDGYPEETNAPYGVAKKALLVQLQAYRQQYGFNGVYLLPVNLYGPEDNFNP
CCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC
QSSHVIPALIRKVYEAQQAGATQLEVWGDGTPTREFLYSEDAARGIIMAMEDYNESDPIN
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEEECCCCCCCEE
LGTNSEVSIRDLVTLICRLMDFKGDVVWLTDKPNGQPRRCLDTAKAKEKFGFTANITLEQ
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHH
GLQNTIDWYRQHPE
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]