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The map label for this gene is rpoD [H]

Identifier: 158337052

GI number: 158337052

Start: 3976790

End: 3977746

Strand: Reverse

Name: rpoD [H]

Synonym: AM1_3925

Alternate gene names: 158337052

Gene position: 3977746-3976790 (Counterclockwise)

Preceding gene: 158337053

Following gene: 158337045

Centisome position: 61.16

GC content: 49.74

Gene sequence:

>957_bases
ATGAGAACCAAAGAAATTGCCAAAGATTCCGTGCGCTCTTACTTGCGAGAGATTGGGCGTGTTCCTCTCTTAACCCATGA
AGAAGAGGTGACGTTTGGCAAGCAGGTCCAACATCTGATCAAGTTGCAAACGATTCGCGATGAATTAGCGGAGCAACTAG
AGCGGCAACCGAACGATGCAGAGTGGTCGGCGGCTGCCAAGCTCTCGGAAGCCGAACTACAAAAGGCTCTACATGAAGGG
GAAGCGGCTAAGCGAAAGATGGTGGAAGCCAATCTACGCTTAGTGGTTTCTGTCGCAAAGAAATATATCAAGCGCAATGT
GGACCTGTTGGATCTGATCCAAGAGGGTACGATTGGCATGCAGCGGGGCGTTGAGAAGTTCGACCCAACTAAAGGATATC
GCTTCTCGACCTATGCCTACTGGTGGATTCGGCAGGCGATCACCCGCGCGATCGCAGAAAAAGGACGAACAATTCGACTC
CCGATCCACATCACGGAAAAGCTCAACAAAATTAAGAAGACGCAACGGCAGCTTTCTCAAAAGTTGGGCCGAGCTGCAAC
CATCAGCGAATTAGCCACGTCTTTAGACTTAACTACAAAGCAAGTCCGAGAATATTTAGAGCGCGCTCGCCAACCTTTAT
CCCTGGATGTTCGGGTTGGGGATAATCAGGATACTGAACTGGGTGAACTAATCGAAGATCCGTCTGAATCTCCTGAGAAT
TTTACCCTACAAGCTTCTTTGCGGGGAGATTTGGAGCGGCTAATGGCAGATTTGACGGACCAGCAGCAGCAGGTTCTCCA
CCTCCGCTTTGGTTTGGATGATGGTCAACCCCTAACCTTAGCTAAGATTGGCGATCGTTTAAGCATCAGCCGGGAAAGGG
TTCGACAGATTGAGCGGGAAGCTCTGAGTAAGCTACGGAAGCGCAAAGCTGATATTTCTGAGTATCTGGCTAGCTAA

Upstream 100 bases:

>100_bases
GCTAAACGGGATTGCCTTTGTAGTGGGACAGATGAGGATTCATTTCTTACAAATCCGCACGCCATCCTAGATGCCTTTGT
CTAAACTTGAGAGACGCCCC

Downstream 100 bases:

>100_bases
CTGCTCACACCTAAAAATGTTCTATTTTTAGGTGCATTGAGGTAATATCTCTCTTCTTTGAACCTTTGGGTAGAAAAAAA
CTTTGGTTTTAAAGTACTCC

Product: RNA polymerase sigma factor SigD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRTKEIAKDSVRSYLREIGRVPLLTHEEEVTFGKQVQHLIKLQTIRDELAEQLERQPNDAEWSAAAKLSEAELQKALHEG
EAAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGMQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIRL
PIHITEKLNKIKKTQRQLSQKLGRAATISELATSLDLTTKQVREYLERARQPLSLDVRVGDNQDTELGELIEDPSESPEN
FTLQASLRGDLERLMADLTDQQQQVLHLRFGLDDGQPLTLAKIGDRLSISRERVRQIEREALSKLRKRKADISEYLAS

Sequences:

>Translated_318_residues
MRTKEIAKDSVRSYLREIGRVPLLTHEEEVTFGKQVQHLIKLQTIRDELAEQLERQPNDAEWSAAAKLSEAELQKALHEG
EAAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGMQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIRL
PIHITEKLNKIKKTQRQLSQKLGRAATISELATSLDLTTKQVREYLERARQPLSLDVRVGDNQDTELGELIEDPSESPEN
FTLQASLRGDLERLMADLTDQQQQVLHLRFGLDDGQPLTLAKIGDRLSISRERVRQIEREALSKLRKRKADISEYLAS
>Mature_318_residues
MRTKEIAKDSVRSYLREIGRVPLLTHEEEVTFGKQVQHLIKLQTIRDELAEQLERQPNDAEWSAAAKLSEAELQKALHEG
EAAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGMQRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIRL
PIHITEKLNKIKKTQRQLSQKLGRAATISELATSLDLTTKQVREYLERARQPLSLDVRVGDNQDTELGELIEDPSESPEN
FTLQASLRGDLERLMADLTDQQQQVLHLRFGLDDGQPLTLAKIGDRLSISRERVRQIEREALSKLRKRKADISEYLAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=240, Percent_Identity=45.8333333333333, Blast_Score=227, Evalue=7e-61,
Organism=Escherichia coli, GI1789098, Length=300, Percent_Identity=38.3333333333333, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1789871, Length=265, Percent_Identity=30.188679245283, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788231, Length=207, Percent_Identity=28.9855072463768, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36520; Mature: 36520

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKEIAKDSVRSYLREIGRVPLLTHEEEVTFGKQVQHLIKLQTIRDELAEQLERQPNDA
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EWSAAAKLSEAELQKALHEGEAAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
QRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIRLPIHITEKLNKIKKTQRQLSQ
HHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHH
KLGRAATISELATSLDLTTKQVREYLERARQPLSLDVRVGDNQDTELGELIEDPSESPEN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCC
FTLQASLRGDLERLMADLTDQQQQVLHLRFGLDDGQPLTLAKIGDRLSISRERVRQIERE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCHHHHHHHHHHHHHHH
ALSKLRKRKADISEYLAS
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTKEIAKDSVRSYLREIGRVPLLTHEEEVTFGKQVQHLIKLQTIRDELAEQLERQPNDA
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EWSAAAKLSEAELQKALHEGEAAKRKMVEANLRLVVSVAKKYIKRNVDLLDLIQEGTIGM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
QRGVEKFDPTKGYRFSTYAYWWIRQAITRAIAEKGRTIRLPIHITEKLNKIKKTQRQLSQ
HHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHH
KLGRAATISELATSLDLTTKQVREYLERARQPLSLDVRVGDNQDTELGELIEDPSESPEN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCC
FTLQASLRGDLERLMADLTDQQQQVLHLRFGLDDGQPLTLAKIGDRLSISRERVRQIERE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCHHHHHHHHHHHHHHH
ALSKLRKRKADISEYLAS
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA