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The map label for this gene is maeB [C]

Identifier: 158337031

GI number: 158337031

Start: 3954117

End: 3955508

Strand: Direct

Name: maeB [C]

Synonym: AM1_3904

Alternate gene names: 158337031

Gene position: 3954117-3955508 (Clockwise)

Preceding gene: 158337029

Following gene: 158337033

Centisome position: 60.8

GC content: 49.71

Gene sequence:

>1392_bases
ATGGTTCAACTGACCCCCAACTCTAGCTTTAGCCTCACGATTCGGGTTAAGTTGCCCAACCAAACGGGAATGCTGGCTCA
AGTCTTGCAGGCAATTGGTGATGCGGGTGGCAGTCTAGGACATATCGATCTTTTAGAACGGACCCATACCTCACTGACTC
GCGAGATTACGGTCAATGCTTCCAGTACAGAGCATGCAGGTACCATTGTTGATGCTTTAAGAGCATTGCCACAGATTACG
TTACTGGATATATCCGATCGCACCTTTGATTGTCACCGAGGCGGAAAAATTACGGTTCAAAGTAAGCTGCCCCTTCATAG
CCAAGATGATTTGTCCATGGCCTATACCCCTGGTGTGGGACGGATTTGTACCGCCATTGCCCAAGAAAAAGATCAGGTGT
ATCGCTACACCATTAAAAGTAATACCGTAGCGGTGGTGACCGATGGTAGTGCGGTGTTGGGTCTCGGAAATATTGGTCCA
GAAGCAGCTTTACCTGTCATGGAAGGTAAGGCCCTACTTTTTCAAGAATTTGGCAAGATTAATGCCTTTCCTGTCTGTCT
AGCCACTCAAGATGTGGATGAGATTGTGGAAACGGTAAAGCGGATGGCTCCCGTTTTTGGTGGCGTCAACCTGGAGGACA
TTAGTGCGCCTCGTTGCTTTGAAGTGGAAGAACGGTTGCGTCGAGAACTGGATATTCCCGTCTTCCATGATGATCAGCAT
GGAACGGCAATTGTGGTGTTAGCGGCGTTAATCAATGCTCTGAAACTGGTGGATAAAGCCTTTGAAGAGATTGCCATTGT
AATTAATGGGGCTGGGGCTGCGGGGGTTGCCATTGCCCGGTTATTACAAAAGGCAGGTGTGAAAACGATTCGATTGTGCG
ATCGCAACGGACTGATCAGCCGAGATCGCACGGATTTAAACGAAACGAAGCGGTCCTTTGCCGTGGAACAGTCAGGTACC
TTAGCCGATGCCCTCAAAGACGCAGATGTATTTCTGGGTGTCAGCGCCCCTGGCGTGTTAACCCCTGAGATGGTCAAGAC
GATGGCATCGGATCGGATTGTGTTTGCCATGGCCAACCCGATTCCTGAAATTCAACCTGAGCTGATTCAAAGTGATGTGG
CTGTGATTGCGACAGGACGGAGTGATTACCCCAACCAAATTAATAACGTCTTGGCGTTTCCTGGAATTTTTCGCGGTGCC
CTGAACTGCCGTGCTGCCGCGATTACAACGACAATGCACCTACAAGCTGCTTATGCGATCGCAGCACTGGTAGGAAGCGA
CGAACTCCACCACGATCACATTATTCCGTCTGTGTTTGATGAGCGCGTCGCCACAGCAGTTGCTTCGGCAGTGGAACAAG
CCGCTCGTCAAGAAGGAATTAGCCATTGTTAA

Upstream 100 bases:

>100_bases
CTCTTCTAGGAATGGGAGAGAACCATCATCATGGTCGTTATATGCCGTTACAATTGGGAGCGGTAGCTTGCAATAAATCT
GACGTACGGTAGATATCATC

Downstream 100 bases:

>100_bases
CGTTTTAAGCAGCGCTGAATATCTAGGAAGGGTATGATTCCACAACCGACTAAAGAGTCTGTATCTTTTTCAGTGCTTGT
ATTGAGCATCTCCAGGAATA

Product: NAD-dependent malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVQLTPNSSFSLTIRVKLPNQTGMLAQVLQAIGDAGGSLGHIDLLERTHTSLTREITVNASSTEHAGTIVDALRALPQIT
LLDISDRTFDCHRGGKITVQSKLPLHSQDDLSMAYTPGVGRICTAIAQEKDQVYRYTIKSNTVAVVTDGSAVLGLGNIGP
EAALPVMEGKALLFQEFGKINAFPVCLATQDVDEIVETVKRMAPVFGGVNLEDISAPRCFEVEERLRRELDIPVFHDDQH
GTAIVVLAALINALKLVDKAFEEIAIVINGAGAAGVAIARLLQKAGVKTIRLCDRNGLISRDRTDLNETKRSFAVEQSGT
LADALKDADVFLGVSAPGVLTPEMVKTMASDRIVFAMANPIPEIQPELIQSDVAVIATGRSDYPNQINNVLAFPGIFRGA
LNCRAAAITTTMHLQAAYAIAALVGSDELHHDHIIPSVFDERVATAVASAVEQAARQEGISHC

Sequences:

>Translated_463_residues
MVQLTPNSSFSLTIRVKLPNQTGMLAQVLQAIGDAGGSLGHIDLLERTHTSLTREITVNASSTEHAGTIVDALRALPQIT
LLDISDRTFDCHRGGKITVQSKLPLHSQDDLSMAYTPGVGRICTAIAQEKDQVYRYTIKSNTVAVVTDGSAVLGLGNIGP
EAALPVMEGKALLFQEFGKINAFPVCLATQDVDEIVETVKRMAPVFGGVNLEDISAPRCFEVEERLRRELDIPVFHDDQH
GTAIVVLAALINALKLVDKAFEEIAIVINGAGAAGVAIARLLQKAGVKTIRLCDRNGLISRDRTDLNETKRSFAVEQSGT
LADALKDADVFLGVSAPGVLTPEMVKTMASDRIVFAMANPIPEIQPELIQSDVAVIATGRSDYPNQINNVLAFPGIFRGA
LNCRAAAITTTMHLQAAYAIAALVGSDELHHDHIIPSVFDERVATAVASAVEQAARQEGISHC
>Mature_463_residues
MVQLTPNSSFSLTIRVKLPNQTGMLAQVLQAIGDAGGSLGHIDLLERTHTSLTREITVNASSTEHAGTIVDALRALPQIT
LLDISDRTFDCHRGGKITVQSKLPLHSQDDLSMAYTPGVGRICTAIAQEKDQVYRYTIKSNTVAVVTDGSAVLGLGNIGP
EAALPVMEGKALLFQEFGKINAFPVCLATQDVDEIVETVKRMAPVFGGVNLEDISAPRCFEVEERLRRELDIPVFHDDQH
GTAIVVLAALINALKLVDKAFEEIAIVINGAGAAGVAIARLLQKAGVKTIRLCDRNGLISRDRTDLNETKRSFAVEQSGT
LADALKDADVFLGVSAPGVLTPEMVKTMASDRIVFAMANPIPEIQPELIQSDVAVIATGRSDYPNQINNVLAFPGIFRGA
LNCRAAAITTTMHLQAAYAIAALVGSDELHHDHIIPSVFDERVATAVASAVEQAARQEGISHC

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=381, Percent_Identity=27.8215223097113, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI4505145, Length=422, Percent_Identity=25.3554502369668, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI62420882, Length=361, Percent_Identity=26.3157894736842, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI62420880, Length=361, Percent_Identity=26.3157894736842, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI239049447, Length=361, Percent_Identity=26.3157894736842, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI270265879, Length=379, Percent_Identity=25.8575197889182, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788806, Length=389, Percent_Identity=46.2724935732648, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI87081919, Length=453, Percent_Identity=25.3863134657837, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322823, Length=332, Percent_Identity=28.0120481927711, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707240, Length=414, Percent_Identity=25.3623188405797, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707242, Length=414, Percent_Identity=25.3623188405797, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707238, Length=414, Percent_Identity=25.3623188405797, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646388, Length=435, Percent_Identity=25.9770114942529, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78707232, Length=427, Percent_Identity=24.1217798594848, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24646386, Length=435, Percent_Identity=25.9770114942529, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281362672, Length=437, Percent_Identity=23.7986270022883, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363503, Length=427, Percent_Identity=24.1217798594848, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281362674, Length=437, Percent_Identity=23.7986270022883, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363505, Length=427, Percent_Identity=24.1217798594848, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78707236, Length=427, Percent_Identity=24.1217798594848, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356279, Length=437, Percent_Identity=23.7986270022883, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 49522; Mature: 49522

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQLTPNSSFSLTIRVKLPNQTGMLAQVLQAIGDAGGSLGHIDLLERTHTSLTREITVNA
CEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEEEEC
SSTEHAGTIVDALRALPQITLLDISDRTFDCHRGGKITVQSKLPLHSQDDLSMAYTPGVG
CCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCCEEECCCHH
RICTAIAQEKDQVYRYTIKSNTVAVVTDGSAVLGLGNIGPEAALPVMEGKALLFQEFGKI
HHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEECCCCCHHCCCHHCCCHHHHHHHCCC
NAFPVCLATQDVDEIVETVKRMAPVFGGVNLEDISAPRCFEVEERLRRELDIPVFHDDQH
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCEEECCCC
GTAIVVLAALINALKLVDKAFEEIAIVINGAGAAGVAIARLLQKAGVKTIRLCDRNGLIS
CHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
RDRTDLNETKRSFAVEQSGTLADALKDADVFLGVSAPGVLTPEMVKTMASDRIVFAMANP
CCCCCHHHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEECCC
IPEIQPELIQSDVAVIATGRSDYPNQINNVLAFPGIFRGALNCRAAAITTTMHLQAAYAI
CCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHH
AALVGSDELHHDHIIPSVFDERVATAVASAVEQAARQEGISHC
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVQLTPNSSFSLTIRVKLPNQTGMLAQVLQAIGDAGGSLGHIDLLERTHTSLTREITVNA
CEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEEEEC
SSTEHAGTIVDALRALPQITLLDISDRTFDCHRGGKITVQSKLPLHSQDDLSMAYTPGVG
CCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCCEEECCCHH
RICTAIAQEKDQVYRYTIKSNTVAVVTDGSAVLGLGNIGPEAALPVMEGKALLFQEFGKI
HHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEECCCCCHHCCCHHCCCHHHHHHHCCC
NAFPVCLATQDVDEIVETVKRMAPVFGGVNLEDISAPRCFEVEERLRRELDIPVFHDDQH
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCEEECCCC
GTAIVVLAALINALKLVDKAFEEIAIVINGAGAAGVAIARLLQKAGVKTIRLCDRNGLIS
CHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
RDRTDLNETKRSFAVEQSGTLADALKDADVFLGVSAPGVLTPEMVKTMASDRIVFAMANP
CCCCCHHHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEECCC
IPEIQPELIQSDVAVIATGRSDYPNQINNVLAFPGIFRGALNCRAAAITTTMHLQAAYAI
CCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHH
AALVGSDELHHDHIIPSVFDERVATAVASAVEQAARQEGISHC
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]