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The map label for this gene is lipA [H]

Identifier: 158336905

GI number: 158336905

Start: 3829405

End: 3830289

Strand: Direct

Name: lipA [H]

Synonym: AM1_3776

Alternate gene names: 158336905

Gene position: 3829405-3830289 (Clockwise)

Preceding gene: 158336900

Following gene: 158336906

Centisome position: 58.88

GC content: 51.07

Gene sequence:

>885_bases
GTGGTTGTTAAGCCCAATTGGTTACGAGTGAAAGCCCCGCAATGGGAGCGTGTGGGCAATGTTAAAGACATTTTACGGGA
CCTACATCTGAATACGGTTTGTGAAGAAGCCTCCTGTCCCAATATTGGAGAGTGTTTCCACCACGGCACGGCGACGTTCT
TAATTATGGGACCAGCCTGCACTCGGGCTTGCCCTTATTGTGATATCGATTTTGAGAAAAAGCCCGTAGCCCTTGACCCC
ACAGAACCCCATCGCTTAGCAGAAGCCGTGCGTCGGATGGGGCTCAATCATGTGGTGATTACCTCGGTCAATCGCGATGA
TTTGCCTGATGGCGGAGCCTCACAGTTTGTTCGCTGTATAGAGCAGATCCGAATCCTGTCCTCCCAGACCACCATTGAAG
TGTTGATTCCTGATTTATGTGGCAACTGGGAAGCCCTAGAGACCATTCTGCAGGCGGCACCGGAAGTGATCAATCACAAT
ACGGAAACCATACCGCGTCTGTATAAGCGGGTTCGCCCTCAAGGAGAGTATCAGCGGACGTTGGATTTGCTGATGCGATC
ACGCCAGCTCGCCCCTTGGATTTACACAAAATCCGGCATCATGGTGGGTCTAGGCGAAACAGATACTGAAGTGCGAGAGG
TCATGGCTGATTTACGCCAAGTGGATTGCGATATTCTCACCATTGGCCAATATTTGCAGCCCACAGCGAAACACCTAGGT
GTGCAAGATTTCGTCCATCCCGATCAATTTGCTACCTGGCAAGCCCTCGGGGAAGAGATGGGCTTTTTGCAGATTGTGTC
CTCCCCCCTCACTCGCAGTTCCTATCATGCCGAGCAAGTCCAAGCCTTAATGGCAAAATATCCTCGTGCTAAACCTGAAC
TATAG

Upstream 100 bases:

>100_bases
AAGCTGGCCTATTCTGGAAGCGCCTATGCCCACCTAGCCGACTTATACCGGAGGAGGCAACATCCCTTACAGTGGAGACA
GAACATTTCAGGTCAGAGTA

Downstream 100 bases:

>100_bases
TGACACTCTAGAAAGTGTCACCTTCTGGTAAAGGCCAGGACCTGCCAGTTTTTTATGCTGGGTGACAGAATTTCATACAC
TTTGTCTCCGCTAGCGAAGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MVVKPNWLRVKAPQWERVGNVKDILRDLHLNTVCEEASCPNIGECFHHGTATFLIMGPACTRACPYCDIDFEKKPVALDP
TEPHRLAEAVRRMGLNHVVITSVNRDDLPDGGASQFVRCIEQIRILSSQTTIEVLIPDLCGNWEALETILQAAPEVINHN
TETIPRLYKRVRPQGEYQRTLDLLMRSRQLAPWIYTKSGIMVGLGETDTEVREVMADLRQVDCDILTIGQYLQPTAKHLG
VQDFVHPDQFATWQALGEEMGFLQIVSSPLTRSSYHAEQVQALMAKYPRAKPEL

Sequences:

>Translated_294_residues
MVVKPNWLRVKAPQWERVGNVKDILRDLHLNTVCEEASCPNIGECFHHGTATFLIMGPACTRACPYCDIDFEKKPVALDP
TEPHRLAEAVRRMGLNHVVITSVNRDDLPDGGASQFVRCIEQIRILSSQTTIEVLIPDLCGNWEALETILQAAPEVINHN
TETIPRLYKRVRPQGEYQRTLDLLMRSRQLAPWIYTKSGIMVGLGETDTEVREVMADLRQVDCDILTIGQYLQPTAKHLG
VQDFVHPDQFATWQALGEEMGFLQIVSSPLTRSSYHAEQVQALMAKYPRAKPEL
>Mature_294_residues
MVVKPNWLRVKAPQWERVGNVKDILRDLHLNTVCEEASCPNIGECFHHGTATFLIMGPACTRACPYCDIDFEKKPVALDP
TEPHRLAEAVRRMGLNHVVITSVNRDDLPDGGASQFVRCIEQIRILSSQTTIEVLIPDLCGNWEALETILQAAPEVINHN
TETIPRLYKRVRPQGEYQRTLDLLMRSRQLAPWIYTKSGIMVGLGETDTEVREVMADLRQVDCDILTIGQYLQPTAKHLG
VQDFVHPDQFATWQALGEEMGFLQIVSSPLTRSSYHAEQVQALMAKYPRAKPEL

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=40.6896551724138, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=40.9836065573771, Blast_Score=214, Evalue=6e-56,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=49.6428571428571, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32564533, Length=252, Percent_Identity=41.2698412698413, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=40.0709219858156, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI221513272, Length=279, Percent_Identity=43.7275985663082, Blast_Score=254, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33226; Mature: 33226

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKPNWLRVKAPQWERVGNVKDILRDLHLNTVCEEASCPNIGECFHHGTATFLIMGPAC
CCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCHH
TRACPYCDIDFEKKPVALDPTEPHRLAEAVRRMGLNHVVITSVNRDDLPDGGASQFVRCI
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
EQIRILSSQTTIEVLIPDLCGNWEALETILQAAPEVINHNTETIPRLYKRVRPQGEYQRT
HHHHHHHCCCEEEEEECHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
LDLLMRSRQLAPWIYTKSGIMVGLGETDTEVREVMADLRQVDCDILTIGQYLQPTAKHLG
HHHHHHHHCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
VQDFVHPDQFATWQALGEEMGFLQIVSSPLTRSSYHAEQVQALMAKYPRAKPEL
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVVKPNWLRVKAPQWERVGNVKDILRDLHLNTVCEEASCPNIGECFHHGTATFLIMGPAC
CCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCHH
TRACPYCDIDFEKKPVALDPTEPHRLAEAVRRMGLNHVVITSVNRDDLPDGGASQFVRCI
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
EQIRILSSQTTIEVLIPDLCGNWEALETILQAAPEVINHNTETIPRLYKRVRPQGEYQRT
HHHHHHHCCCEEEEEECHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
LDLLMRSRQLAPWIYTKSGIMVGLGETDTEVREVMADLRQVDCDILTIGQYLQPTAKHLG
HHHHHHHHCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
VQDFVHPDQFATWQALGEEMGFLQIVSSPLTRSSYHAEQVQALMAKYPRAKPEL
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]