Click here to switch to the map view.

The map label for this gene is sphR [H]

Identifier: 158336857

GI number: 158336857

Start: 3781998

End: 3782798

Strand: Reverse

Name: sphR [H]

Synonym: AM1_3725

Alternate gene names: 158336857

Gene position: 3782798-3781998 (Counterclockwise)

Preceding gene: 158336858

Following gene: 158336855

Centisome position: 58.16

GC content: 50.31

Gene sequence:

>801_bases
GTGGACTCACTCCACATCCTAATCGTTGAGGCTAATCCTCACTTGCGATCTCTTCTGGGTTGGCATCTACAACAAGTAGG
CTATTTTATTCATCTCAGCGCTAGTATCAAGCAAGCTCAGGCTGCCTATCAACAACATCAACCGGATCTGGTTATCGTTG
ATTCTGAATTACCCGATGGCCTGGGTCAGGAGCTTTGTCGATGGTTACAGCGCCAAAAGCATCCCCTGATTTTGATGTTG
TCGGCCCAAACAACTGAAGCAGATGTCGTATCTGGCCTCAAAGCCGGTGCAGATGATTATGTGGGTAAGCCTTTTGGGAT
GCAAGAGTTTTTGGCTCGGATTGCGGCTTTGAGCCGACGCAGCCGCCTCAGCCTGCCGACGCAGGTTTCATTTGGTGCCT
TGCGAATTGATTTGATCCAGCGGCGCGTTCACTACCATAACGAGCTAGTTGATCTCACTCCCCAGGAATTTAGCCTGCTG
TATGTCTTAGTTCAAGCAGAAGGCCTAGCCCTCAGCCGGGTTGAACTCTTGCAACGAGCCTGGCCCGAAAGTATTGATAA
TCCCCGAACGGTGGATACTCATATCTTGTCCCTCCGCAAAAAGATCGAACAAGATCCCCAACAGCCTCAACTGATTCAAA
CAGTCCGGAACGTGGGCTATCGCCTCAATCTTGGATATTTAATGGATGCCAATCCATCCTACAGGTCTGATTCGTCGCAG
ACCTCACCCCCATCCAATCGTTTTTTGGCAGATGGGACGACTACCAGCCAAATGCCCATGTCCCGAGCCCAATCGGGATA
A

Upstream 100 bases:

>100_bases
TCCAAAATCTCATTACTGGGCCTGTTCGTAACCACAGTTACCAAAACCTGAAAATTTATCTGCTAACGTTGGGGTAGCAG
AATGCAGGACCCTAAATTCC

Downstream 100 bases:

>100_bases
TTGTGATCGCCTCCTAGTGCATCGTCAAGACTTAATTTTAGGGTAAGCACCTCTGGTAAGCCTTATGAAGAAAGCATCCT
GAAGATCGTCCACCCTAATC

Product: two-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MDSLHILIVEANPHLRSLLGWHLQQVGYFIHLSASIKQAQAAYQQHQPDLVIVDSELPDGLGQELCRWLQRQKHPLILML
SAQTTEADVVSGLKAGADDYVGKPFGMQEFLARIAALSRRSRLSLPTQVSFGALRIDLIQRRVHYHNELVDLTPQEFSLL
YVLVQAEGLALSRVELLQRAWPESIDNPRTVDTHILSLRKKIEQDPQQPQLIQTVRNVGYRLNLGYLMDANPSYRSDSSQ
TSPPSNRFLADGTTTSQMPMSRAQSG

Sequences:

>Translated_266_residues
MDSLHILIVEANPHLRSLLGWHLQQVGYFIHLSASIKQAQAAYQQHQPDLVIVDSELPDGLGQELCRWLQRQKHPLILML
SAQTTEADVVSGLKAGADDYVGKPFGMQEFLARIAALSRRSRLSLPTQVSFGALRIDLIQRRVHYHNELVDLTPQEFSLL
YVLVQAEGLALSRVELLQRAWPESIDNPRTVDTHILSLRKKIEQDPQQPQLIQTVRNVGYRLNLGYLMDANPSYRSDSSQ
TSPPSNRFLADGTTTSQMPMSRAQSG
>Mature_266_residues
MDSLHILIVEANPHLRSLLGWHLQQVGYFIHLSASIKQAQAAYQQHQPDLVIVDSELPDGLGQELCRWLQRQKHPLILML
SAQTTEADVVSGLKAGADDYVGKPFGMQEFLARIAALSRRSRLSLPTQVSFGALRIDLIQRRVHYHNELVDLTPQEFSLL
YVLVQAEGLALSRVELLQRAWPESIDNPRTVDTHILSLRKKIEQDPQQPQLIQTVRNVGYRLNLGYLMDANPSYRSDSSQ
TSPPSNRFLADGTTTSQMPMSRAQSG

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=36.322869955157, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=34.6491228070175, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=34.8214285714286, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788394, Length=220, Percent_Identity=35.4545454545455, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=33.1858407079646, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=33.4821428571429, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=34.6846846846847, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=30.4932735426009, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=30.7339449541284, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=32.7354260089686, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787229, Length=210, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=29.6137339055794, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=30.6666666666667, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI145693140, Length=228, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 29941; Mature: 29941

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLHILIVEANPHLRSLLGWHLQQVGYFIHLSASIKQAQAAYQQHQPDLVIVDSELPDG
CCCEEEEEEECCHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCH
LGQELCRWLQRQKHPLILMLSAQTTEADVVSGLKAGADDYVGKPFGMQEFLARIAALSRR
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
SRLSLPTQVSFGALRIDLIQRRVHYHNELVDLTPQEFSLLYVLVQAEGLALSRVELLQRA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHHHHH
WPESIDNPRTVDTHILSLRKKIEQDPQQPQLIQTVRNVGYRLNLGYLMDANPSYRSDSSQ
CHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCEEEECCCCCCCCCCC
TSPPSNRFLADGTTTSQMPMSRAQSG
CCCCCCCEEECCCCCCCCCHHHCCCC
>Mature Secondary Structure
MDSLHILIVEANPHLRSLLGWHLQQVGYFIHLSASIKQAQAAYQQHQPDLVIVDSELPDG
CCCEEEEEEECCHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCH
LGQELCRWLQRQKHPLILMLSAQTTEADVVSGLKAGADDYVGKPFGMQEFLARIAALSRR
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
SRLSLPTQVSFGALRIDLIQRRVHYHNELVDLTPQEFSLLYVLVQAEGLALSRVELLQRA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHHHHH
WPESIDNPRTVDTHILSLRKKIEQDPQQPQLIQTVRNVGYRLNLGYLMDANPSYRSDSSQ
CHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCEEEECCCCCCCCCCC
TSPPSNRFLADGTTTSQMPMSRAQSG
CCCCCCCEEECCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]