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The map label for this gene is dus2 [H]

Identifier: 158336790

GI number: 158336790

Start: 3714416

End: 3715423

Strand: Reverse

Name: dus2 [H]

Synonym: AM1_3658

Alternate gene names: 158336790

Gene position: 3715423-3714416 (Counterclockwise)

Preceding gene: 158336791

Following gene: 158336788

Centisome position: 57.13

GC content: 46.33

Gene sequence:

>1008_bases
ATGGCTTTCTCATCCCCTTCTAAACGTCATTATCCCGTCAGTGTCGCTCCCATGATGGATCGGACGGATCGGCATTTCCG
TTACTTTATGCGGCAAATCACCCAACGGACTTTGCTCTATACAGAGATGGTAACGAGTGCTGCGATTTTAAATGGCGACC
GCCACCATTTACTCGATTTTTCTCCCATTGAAAAACCCTTGGTCTTGCAAGTAGGCGGCGATAATCCCCAAGATTTAGCT
GAATGTGCTCGTATTGCCACAGACTGGGGATATGACGAAATTAATTTAAATGTGGGCTGTCCCAGTAGTCGAGTTCAAAA
CGGTCATTTTGGGGCTTGTTTAATGGCTCAGCCAGATCGGGTAGCAGCCTGCGTGGAAGCCATGATGCAGGCAACTCACC
TACCTGTCTCCGTCAAGCATCGCATTGGTATTGATGATTGCGATCGCTATGAAGATATGGCCCACTTTGTCCAGACCGTT
GCTGCGTCAGGCTGTCAAATCTTTAGTGTTCATGCCCGCAAAGCTTGGCTAAAAGGCTTAAGTCCTAAGGAAAACCGTGA
CATCCCGCCTTTACGTTACGAAGATGTTTATCGATTAAAACAAAACTTTTCTCATCTCTGGATTGAAATTAACGGTGGAT
TCATCCAGTTATTCCAGGTGCACAATCAATTACCAAAAGTAGACGCAGTCATGATTGGTCGGGCTGCCTATGATAATCCT
TACCTATTTGCGACAGTCGATCGCGACTTTTATCACGAAGATCGTTCTGCCCCTTCTAGACACAAAGTTGCACAAGCCAT
GATGGACTATTTAGAGGAATGGAAAATAAAAGGGCTATTAAAACCCCACTCCATTACCCGCCATATGCTGCAATTATTTT
CAGGTCAACCTGGTAGCCGGGCTTGGAAGCGGCTATTAACTGAGCAAGGGTGCCGCCCAGATGCAGGCCCAGACATTATC
CATCAGGCATTAGACAAAGTCTTAGCAATACCAGGATTGACCCAGTAA

Upstream 100 bases:

>100_bases
CCATCCCAATCACTCCAACTTCAGCTCATCCTCAACCCAGATGGCACTGTGCAAATATTGGAGGATGTAGCTGGAGGCAA
AGCTCCCTAAACTGATTAAT

Downstream 100 bases:

>100_bases
ACCACAATATCAGCTGGTAATTTACTTTTCTTTTTTCCCTGGGACGCGCTCATAGCCAGGCTTCGGCTTCGTCATTTTGG
CCCATAACCCCTTGACATCA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAFSSPSKRHYPVSVAPMMDRTDRHFRYFMRQITQRTLLYTEMVTSAAILNGDRHHLLDFSPIEKPLVLQVGGDNPQDLA
ECARIATDWGYDEINLNVGCPSSRVQNGHFGACLMAQPDRVAACVEAMMQATHLPVSVKHRIGIDDCDRYEDMAHFVQTV
AASGCQIFSVHARKAWLKGLSPKENRDIPPLRYEDVYRLKQNFSHLWIEINGGFIQLFQVHNQLPKVDAVMIGRAAYDNP
YLFATVDRDFYHEDRSAPSRHKVAQAMMDYLEEWKIKGLLKPHSITRHMLQLFSGQPGSRAWKRLLTEQGCRPDAGPDII
HQALDKVLAIPGLTQ

Sequences:

>Translated_335_residues
MAFSSPSKRHYPVSVAPMMDRTDRHFRYFMRQITQRTLLYTEMVTSAAILNGDRHHLLDFSPIEKPLVLQVGGDNPQDLA
ECARIATDWGYDEINLNVGCPSSRVQNGHFGACLMAQPDRVAACVEAMMQATHLPVSVKHRIGIDDCDRYEDMAHFVQTV
AASGCQIFSVHARKAWLKGLSPKENRDIPPLRYEDVYRLKQNFSHLWIEINGGFIQLFQVHNQLPKVDAVMIGRAAYDNP
YLFATVDRDFYHEDRSAPSRHKVAQAMMDYLEEWKIKGLLKPHSITRHMLQLFSGQPGSRAWKRLLTEQGCRPDAGPDII
HQALDKVLAIPGLTQ
>Mature_334_residues
AFSSPSKRHYPVSVAPMMDRTDRHFRYFMRQITQRTLLYTEMVTSAAILNGDRHHLLDFSPIEKPLVLQVGGDNPQDLAE
CARIATDWGYDEINLNVGCPSSRVQNGHFGACLMAQPDRVAACVEAMMQATHLPVSVKHRIGIDDCDRYEDMAHFVQTVA
ASGCQIFSVHARKAWLKGLSPKENRDIPPLRYEDVYRLKQNFSHLWIEINGGFIQLFQVHNQLPKVDAVMIGRAAYDNPY
LFATVDRDFYHEDRSAPSRHKVAQAMMDYLEEWKIKGLLKPHSITRHMLQLFSGQPGSRAWKRLLTEQGCRPDAGPDIIH
QALDKVLAIPGLTQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=28.3898305084746, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40807366, Length=268, Percent_Identity=27.2388059701493, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=51.4285714285714, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789660, Length=235, Percent_Identity=25.9574468085106, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788462, Length=256, Percent_Identity=28.90625, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=26.3473053892216, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=170, Percent_Identity=31.1764705882353, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24580595, Length=287, Percent_Identity=27.8745644599303, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=287, Percent_Identity=27.8745644599303, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38107; Mature: 37976

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSSPSKRHYPVSVAPMMDRTDRHFRYFMRQITQRTLLYTEMVTSAAILNGDRHHLLDF
CCCCCCCCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
SPIEKPLVLQVGGDNPQDLAECARIATDWGYDEINLNVGCPSSRVQNGHFGACLMAQPDR
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCCEEEECCCHH
VAACVEAMMQATHLPVSVKHRIGIDDCDRYEDMAHFVQTVAASGCQIFSVHARKAWLKGL
HHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCC
SPKENRDIPPLRYEDVYRLKQNFSHLWIEINGGFIQLFQVHNQLPKVDAVMIGRAAYDNP
CCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCEEHHHHHHHHCCCHHHHEECCCCCCCC
YLFATVDRDFYHEDRSAPSRHKVAQAMMDYLEEWKIKGLLKPHSITRHMLQLFSGQPGSR
EEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
AWKRLLTEQGCRPDAGPDIIHQALDKVLAIPGLTQ
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AFSSPSKRHYPVSVAPMMDRTDRHFRYFMRQITQRTLLYTEMVTSAAILNGDRHHLLDF
CCCCCCCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
SPIEKPLVLQVGGDNPQDLAECARIATDWGYDEINLNVGCPSSRVQNGHFGACLMAQPDR
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCCEEEECCCHH
VAACVEAMMQATHLPVSVKHRIGIDDCDRYEDMAHFVQTVAASGCQIFSVHARKAWLKGL
HHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCC
SPKENRDIPPLRYEDVYRLKQNFSHLWIEINGGFIQLFQVHNQLPKVDAVMIGRAAYDNP
CCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCEEHHHHHHHHCCCHHHHEECCCCCCCC
YLFATVDRDFYHEDRSAPSRHKVAQAMMDYLEEWKIKGLLKPHSITRHMLQLFSGQPGSR
EEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
AWKRLLTEQGCRPDAGPDIIHQALDKVLAIPGLTQ
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]