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The map label for this gene is pyr [H]

Identifier: 158336714

GI number: 158336714

Start: 3630161

End: 3631435

Strand: Reverse

Name: pyr [H]

Synonym: AM1_3582

Alternate gene names: 158336714

Gene position: 3631435-3630161 (Counterclockwise)

Preceding gene: 158336715

Following gene: 158336713

Centisome position: 55.84

GC content: 53.65

Gene sequence:

>1275_bases
ATGACACAAGAACAGCTCAAACAAGTGCGCGTGTTAGATCCACCGTCACAAGTTGATCGGGTGGCTGATGTCCTGATTGA
CAATGGCAAAATCATAGAGATTTCAGAGCCGGGTGTCAGCACGGTTTCAGGCAGTCAAGAACGAGATTGCTCAGGGCTGA
TTTTGGGGCCGGGGCTGGTCGATCTCTATAGCCATTCCGATGATCCAGGGTTTGAAACGCGGGAGACCTTGGCGTCTTTG
ACTGCATCTGCGATCGCAGGCGGTTTCACGCGCCTCACCCTTCTCCCCGATACCCATCCGCCACTGGATAATGGCGATGG
TTTAGCCCGGATCCAAGCTCAGCTTCCCCCCGGGTTACCCCTGCAAGTCCAAGCTTGGGGGGCCATGACCCAAAACCTAG
AAGGCAAACAGCTCGTTGAATATGCAGATTTGATTCAGGCTGGGGTGGTCGGGTTTAGTGATGGCCGATCGCTTCAGAAC
GCCATCTTGCTGCGGCGACTCTGTGAATATCTAGCGGCAGAGTCTTACCCTATCGCCCTTTGGCCTTGCGATCCTGCCTT
AGCGGGTTGTGGCGTTATCCGCGAAGGCCTCGATGCCCTGCGATTAGGGCTATCAGAGTCCCCCACCATGGCTGAGACAT
CGGTCCTGGCTGCCCTGTTAGAAATCATCTCTGCAACTCAGGTGCCTGTCCATATTATGAGGGTGTCTACGGCTCGAAGT
GTCGAACTAATTCAAGCTGCCAAGGCTCGTGGACTACCGATCACAGCCAGTACCACCTGGATGCATGTGTTAGGCAATAG
CCAGTCTCTCAGTACCTATGATGTCAACTGGCGATTAGATCCTCCTGTTGGCAATCTCCAAGATCAACAGGCTTTGATAC
AGGGATTAGAAACGGGCATCATCGATGCGATCGCAATTGATCACCATGCCTATACTTATGAAGAAAAAACCGTTGCCTTT
AGTGCGGCTCCTCCGGGCGCAATTGGTTTAGAGCTGGCATTATCCTTACTGTGGCAGTCCTTGGTCGCCAGCAAGCGCTG
GTCACCCCTCACCCTATGGCAACGGCTCAGTACTGCTCCTAGCCACTGTTTAGGACAAGCACCTCCTAGAGTGGAAGTGG
GTCAGCCCGCAGAAATGGTGCTCTTTGATCCTCAACAGTCCTGGTCCGCTGACGCCCACACCTTAAAATCCCTGAGTCAG
AATACCCCCTGGTATGGACAGTCCCTGACAGGTCGGGTGATACAAACCTGGCATCCCCCATCGCAAATCCGTTAA

Upstream 100 bases:

>100_bases
CCCATTTAGCAGGCGGCGTCTTAGACCAAACAGCAGCAGGGGCGCTATAACCGGCATCCATCTTTAGTCTTGATCTAGGA
GAACCCACGACGGATCGGGT

Downstream 100 bases:

>100_bases
AGTGTCGGTAAGGGGCTAGACGGTTATCCTGGGACGACGGACCTCTAGCCAATTCGATGAGGGCGTGAACCATGTGTATT
TGCATCAATTGCCAATTCGT

Product: dihydroorotase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 42 kDa non-catalytic chain [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTQEQLKQVRVLDPPSQVDRVADVLIDNGKIIEISEPGVSTVSGSQERDCSGLILGPGLVDLYSHSDDPGFETRETLASL
TASAIAGGFTRLTLLPDTHPPLDNGDGLARIQAQLPPGLPLQVQAWGAMTQNLEGKQLVEYADLIQAGVVGFSDGRSLQN
AILLRRLCEYLAAESYPIALWPCDPALAGCGVIREGLDALRLGLSESPTMAETSVLAALLEIISATQVPVHIMRVSTARS
VELIQAAKARGLPITASTTWMHVLGNSQSLSTYDVNWRLDPPVGNLQDQQALIQGLETGIIDAIAIDHHAYTYEEKTVAF
SAAPPGAIGLELALSLLWQSLVASKRWSPLTLWQRLSTAPSHCLGQAPPRVEVGQPAEMVLFDPQQSWSADAHTLKSLSQ
NTPWYGQSLTGRVIQTWHPPSQIR

Sequences:

>Translated_424_residues
MTQEQLKQVRVLDPPSQVDRVADVLIDNGKIIEISEPGVSTVSGSQERDCSGLILGPGLVDLYSHSDDPGFETRETLASL
TASAIAGGFTRLTLLPDTHPPLDNGDGLARIQAQLPPGLPLQVQAWGAMTQNLEGKQLVEYADLIQAGVVGFSDGRSLQN
AILLRRLCEYLAAESYPIALWPCDPALAGCGVIREGLDALRLGLSESPTMAETSVLAALLEIISATQVPVHIMRVSTARS
VELIQAAKARGLPITASTTWMHVLGNSQSLSTYDVNWRLDPPVGNLQDQQALIQGLETGIIDAIAIDHHAYTYEEKTVAF
SAAPPGAIGLELALSLLWQSLVASKRWSPLTLWQRLSTAPSHCLGQAPPRVEVGQPAEMVLFDPQQSWSADAHTLKSLSQ
NTPWYGQSLTGRVIQTWHPPSQIR
>Mature_423_residues
TQEQLKQVRVLDPPSQVDRVADVLIDNGKIIEISEPGVSTVSGSQERDCSGLILGPGLVDLYSHSDDPGFETRETLASLT
ASAIAGGFTRLTLLPDTHPPLDNGDGLARIQAQLPPGLPLQVQAWGAMTQNLEGKQLVEYADLIQAGVVGFSDGRSLQNA
ILLRRLCEYLAAESYPIALWPCDPALAGCGVIREGLDALRLGLSESPTMAETSVLAALLEIISATQVPVHIMRVSTARSV
ELIQAAKARGLPITASTTWMHVLGNSQSLSTYDVNWRLDPPVGNLQDQQALIQGLETGIIDAIAIDHHAYTYEEKTVAFS
AAPPGAIGLELALSLLWQSLVASKRWSPLTLWQRLSTAPSHCLGQAPPRVEVGQPAEMVLFDPQQSWSADAHTLKSLSQN
TPWYGQSLTGRVIQTWHPPSQIR

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=372, Percent_Identity=30.1075268817204, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1786722, Length=417, Percent_Identity=22.3021582733813, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082175, Length=418, Percent_Identity=24.8803827751196, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=373, Percent_Identity=26.5415549597855, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6322218, Length=381, Percent_Identity=24.6719160104987, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004722 [H]

Pfam domain/function: NA

EC number: 3.5.2.3

Molecular weight: Translated: 45697; Mature: 45566

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEQLKQVRVLDPPSQVDRVADVLIDNGKIIEISEPGVSTVSGSQERDCSGLILGPGLV
CCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCHH
DLYSHSDDPGFETRETLASLTASAIAGGFTRLTLLPDTHPPLDNGDGLARIQAQLPPGLP
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCC
LQVQAWGAMTQNLEGKQLVEYADLIQAGVVGFSDGRSLQNAILLRRLCEYLAAESYPIAL
EEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
WPCDPALAGCGVIREGLDALRLGLSESPTMAETSVLAALLEIISATQVPVHIMRVSTARS
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHH
VELIQAAKARGLPITASTTWMHVLGNSQSLSTYDVNWRLDPPVGNLQDQQALIQGLETGI
HHHHHHHHHCCCEEEECCCEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCC
IDAIAIDHHAYTYEEKTVAFSAAPPGAIGLELALSLLWQSLVASKRWSPLTLWQRLSTAP
EEEEEECCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCH
SHCLGQAPPRVEVGQPAEMVLFDPQQSWSADAHTLKSLSQNTPWYGQSLTGRVIQTWHPP
HHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCH
SQIR
HCCC
>Mature Secondary Structure 
TQEQLKQVRVLDPPSQVDRVADVLIDNGKIIEISEPGVSTVSGSQERDCSGLILGPGLV
CHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCHH
DLYSHSDDPGFETRETLASLTASAIAGGFTRLTLLPDTHPPLDNGDGLARIQAQLPPGLP
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCC
LQVQAWGAMTQNLEGKQLVEYADLIQAGVVGFSDGRSLQNAILLRRLCEYLAAESYPIAL
EEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
WPCDPALAGCGVIREGLDALRLGLSESPTMAETSVLAALLEIISATQVPVHIMRVSTARS
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHH
VELIQAAKARGLPITASTTWMHVLGNSQSLSTYDVNWRLDPPVGNLQDQQALIQGLETGI
HHHHHHHHHCCCEEEECCCEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCC
IDAIAIDHHAYTYEEKTVAFSAAPPGAIGLELALSLLWQSLVASKRWSPLTLWQRLSTAP
EEEEEECCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCH
SHCLGQAPPRVEVGQPAEMVLFDPQQSWSADAHTLKSLSQNTPWYGQSLTGRVIQTWHPP
HHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCH
SQIR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]