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The map label for this gene is dfrA [H]

Identifier: 158336689

GI number: 158336689

Start: 3600230

End: 3601297

Strand: Reverse

Name: dfrA [H]

Synonym: AM1_3556

Alternate gene names: 158336689

Gene position: 3601297-3600230 (Counterclockwise)

Preceding gene: 158336691

Following gene: 158336685

Centisome position: 55.37

GC content: 49.81

Gene sequence:

>1068_bases
ATGACCAACCCCACCTACGATACATCAGCCCCCGTTCTCGTCACTGGAGCGACCGGTTATGTCGCCGGATGGCTCGTCAA
GCGTTTACTGGAAGAAGGCTTTACCGTTCACGCAGCTGTTCGCAATCCCAGCAATACTGAGAAGCTTAAATACCTTAATG
CTTTGGCAGATCAGTCTCCAGGGCAGCTCCAATATTTCCAGTCAGATTTACTGACGCTAGGATCTTACGCCGAGGCCATG
CAGGGCTGTACCGTAGTGTTTCACACGGCTTCTCCTTTCACTATTGCCGTTGATAATCCACAAAAAGATCTGATTGAACC
CGCCCAGCTCGGCACCCGCAATGTTTTAGAACAAGCCAATCAAACTGAGTCTGTGAAGCGGGTGGTGGTCACCAGCAGTT
GTGCCGCCATTTATGGCGATAGTGCAGACTTAGAAATGGTTCCAGGCGATTTATTTACTGAAGCAGACTGGAATACCACC
TCCTCCCTCACCCATCAGCCCTATTCCTATTCCAAAACCTTAGCTGAGCAAGAAGCTTGGAAAATTGCCAAGACCCAGGA
CCGCTGGAACTTAGTCACCATCAATCCCTCCCTCGTTTTAGGACCTGGGATTAACCCTTTTGCCACCTCAGAGTCTTTCT
CGCTGATCAAACAGCTGGGAGATGGCACGATGAAAATGGGCGTACCAAACTATCAGATGGGGGTTGTGGACGTTCGGGAT
GTCGCAGAAGCGCATCTGGCTGCAGGATTTACTCCCAATGCCCAAGGCCGCCATATCACCTCTGGATACAACACAAATTT
CCCTCAAATGGCTGAGATTTTGCGGGCTCATTTTGGCGAGGCTTATCCCTTCCCCAAAAGTGTGCTGCCCAAAGCATTGA
TTTGGCTGGTGGGACCGTTATTAGATAGCAGCATGACGCGCAAAATCATAGCTAGAAACGTGGGTGTTCCTTTTGGTGTC
GATAACAGCAAGAGCATTCAGAAGCTAGGACTCACCTATCGTCCCTTGGCCACATCCCTCACTGATATGTTTCAGCAGTT
GATTGACTATGACGTTGTGACGACTTGA

Upstream 100 bases:

>100_bases
CAAGTGACCCAGAACCTCTAGCTCCAGCATCCACCCAGCGAATACGCTACTCTACGGGATAACGGTATTTCGACCAATTC
TGCAGAATTAGGGAAGCAGT

Downstream 100 bases:

>100_bases
ACTTGAGGTATGGCAAAGGTGCACCCTGTTAATTCTTCTGAAACATCCCAGAGCTGGGCAGCAGCCGCTTGATTTTGGGC
ATAGTCAGACTGCGTAGCCC

Product: NAD-dependent epimerase/dehydratase family protein

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTNPTYDTSAPVLVTGATGYVAGWLVKRLLEEGFTVHAAVRNPSNTEKLKYLNALADQSPGQLQYFQSDLLTLGSYAEAM
QGCTVVFHTASPFTIAVDNPQKDLIEPAQLGTRNVLEQANQTESVKRVVVTSSCAAIYGDSADLEMVPGDLFTEADWNTT
SSLTHQPYSYSKTLAEQEAWKIAKTQDRWNLVTINPSLVLGPGINPFATSESFSLIKQLGDGTMKMGVPNYQMGVVDVRD
VAEAHLAAGFTPNAQGRHITSGYNTNFPQMAEILRAHFGEAYPFPKSVLPKALIWLVGPLLDSSMTRKIIARNVGVPFGV
DNSKSIQKLGLTYRPLATSLTDMFQQLIDYDVVTT

Sequences:

>Translated_355_residues
MTNPTYDTSAPVLVTGATGYVAGWLVKRLLEEGFTVHAAVRNPSNTEKLKYLNALADQSPGQLQYFQSDLLTLGSYAEAM
QGCTVVFHTASPFTIAVDNPQKDLIEPAQLGTRNVLEQANQTESVKRVVVTSSCAAIYGDSADLEMVPGDLFTEADWNTT
SSLTHQPYSYSKTLAEQEAWKIAKTQDRWNLVTINPSLVLGPGINPFATSESFSLIKQLGDGTMKMGVPNYQMGVVDVRD
VAEAHLAAGFTPNAQGRHITSGYNTNFPQMAEILRAHFGEAYPFPKSVLPKALIWLVGPLLDSSMTRKIIARNVGVPFGV
DNSKSIQKLGLTYRPLATSLTDMFQQLIDYDVVTT
>Mature_354_residues
TNPTYDTSAPVLVTGATGYVAGWLVKRLLEEGFTVHAAVRNPSNTEKLKYLNALADQSPGQLQYFQSDLLTLGSYAEAMQ
GCTVVFHTASPFTIAVDNPQKDLIEPAQLGTRNVLEQANQTESVKRVVVTSSCAAIYGDSADLEMVPGDLFTEADWNTTS
SLTHQPYSYSKTLAEQEAWKIAKTQDRWNLVTINPSLVLGPGINPFATSESFSLIKQLGDGTMKMGVPNYQMGVVDVRDV
AEAHLAAGFTPNAQGRHITSGYNTNFPQMAEILRAHFGEAYPFPKSVLPKALIWLVGPLLDSSMTRKIIARNVGVPFGVD
NSKSIQKLGLTYRPLATSLTDMFQQLIDYDVVTT

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=354, Percent_Identity=33.8983050847458, Blast_Score=157, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6320750, Length=360, Percent_Identity=29.7222222222222, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321281, Length=365, Percent_Identity=29.5890410958904, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324421, Length=359, Percent_Identity=28.9693593314763, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321399, Length=357, Percent_Identity=28.0112044817927, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 38732; Mature: 38601

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPTYDTSAPVLVTGATGYVAGWLVKRLLEEGFTVHAAVRNPSNTEKLKYLNALADQSP
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCC
GQLQYFQSDLLTLGSYAEAMQGCTVVFHTASPFTIAVDNPQKDLIEPAQLGTRNVLEQAN
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHCCHHHCCHHHHHHHCC
QTESVKRVVVTSSCAAIYGDSADLEMVPGDLFTEADWNTTSSLTHQPYSYSKTLAEQEAW
HHHHHHHHHHCCCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KIAKTQDRWNLVTINPSLVLGPGINPFATSESFSLIKQLGDGTMKMGVPNYQMGVVDVRD
HHHCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEECCEEHHH
VAEAHLAAGFTPNAQGRHITSGYNTNFPQMAEILRAHFGEAYPFPKSVLPKALIWLVGPL
HHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LDSSMTRKIIARNVGVPFGVDNSKSIQKLGLTYRPLATSLTDMFQQLIDYDVVTT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNPTYDTSAPVLVTGATGYVAGWLVKRLLEEGFTVHAAVRNPSNTEKLKYLNALADQSP
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCC
GQLQYFQSDLLTLGSYAEAMQGCTVVFHTASPFTIAVDNPQKDLIEPAQLGTRNVLEQAN
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHCCHHHCCHHHHHHHCC
QTESVKRVVVTSSCAAIYGDSADLEMVPGDLFTEADWNTTSSLTHQPYSYSKTLAEQEAW
HHHHHHHHHHCCCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KIAKTQDRWNLVTINPSLVLGPGINPFATSESFSLIKQLGDGTMKMGVPNYQMGVVDVRD
HHHCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEECCEEHHH
VAEAHLAAGFTPNAQGRHITSGYNTNFPQMAEILRAHFGEAYPFPKSVLPKALIWLVGPL
HHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LDSSMTRKIIARNVGVPFGVDNSKSIQKLGLTYRPLATSLTDMFQQLIDYDVVTT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]