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The map label for this gene is def [H]

Identifier: 158336649

GI number: 158336649

Start: 3558468

End: 3558998

Strand: Direct

Name: def [H]

Synonym: AM1_3517

Alternate gene names: 158336649

Gene position: 3558468-3558998 (Clockwise)

Preceding gene: 158336648

Following gene: 158336651

Centisome position: 54.71

GC content: 47.27

Gene sequence:

>531_bases
ATGTCAGCGATGCAACGAGAAGTTCTCCAACTTGGTAATCCTAAACTGCGTCAGACAGCCTGTCCTGTGCAAAATCCCAC
CGATTTAGAGATTCAGTCTCTAATTGATGACTTAATTGGAACCATGGAGGAGTCCAATGGCGTTGGCATTGCTGCTCCTC
AAGTAGGCATTGATTTGCAAGTAGTCATTGTGGCCTCCCGCCCCACTCTCAGATATCCTCATGCGCCTCACATGGATCCG
ATTGCCATGATTAATCCCCACATCACCCCTATCGGTGCAGAGCGGGTGAAGGACTGGGAAGGCTGTTTGAGTGTACCGGG
TATTCGAGGATTAGTTCCTCGTGCTTCTACCATTTCTGTTGAGTACCAAGATCGCAACGGTCAGGCCCAAACAGAAATCT
TTCAGGACTTTGTGGCACGCATTATTCAGCATGAATGCGACCATTTACAGGGCTATGTGTTTATTGACAGAGTTGAATCC
ACTCGGGAACTTATTACCGAAAATGAGTATCACAAGCTAGTACTTTCCTAG

Upstream 100 bases:

>100_bases
GACACTGAAATCGTAATATTCACGCTTATGTTTGGATACGACAGTCCGGGTTAAGCAAAGTTAAAATTGAACCACTTTTA
AGCAGAAGGGAGAGTCCGTC

Downstream 100 bases:

>100_bases
TTTTGGAGGCTCAAAGGTCATAAATAGTGTGCAATTTTATTCCAAAGTATAATTTTTGACGGTCATACGTTATACTCATC
CAAACTTAGTTTTTCATTGA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSAMQREVLQLGNPKLRQTACPVQNPTDLEIQSLIDDLIGTMEESNGVGIAAPQVGIDLQVVIVASRPTLRYPHAPHMDP
IAMINPHITPIGAERVKDWEGCLSVPGIRGLVPRASTISVEYQDRNGQAQTEIFQDFVARIIQHECDHLQGYVFIDRVES
TRELITENEYHKLVLS

Sequences:

>Translated_176_residues
MSAMQREVLQLGNPKLRQTACPVQNPTDLEIQSLIDDLIGTMEESNGVGIAAPQVGIDLQVVIVASRPTLRYPHAPHMDP
IAMINPHITPIGAERVKDWEGCLSVPGIRGLVPRASTISVEYQDRNGQAQTEIFQDFVARIIQHECDHLQGYVFIDRVES
TRELITENEYHKLVLS
>Mature_175_residues
SAMQREVLQLGNPKLRQTACPVQNPTDLEIQSLIDDLIGTMEESNGVGIAAPQVGIDLQVVIVASRPTLRYPHAPHMDPI
AMINPHITPIGAERVKDWEGCLSVPGIRGLVPRASTISVEYQDRNGQAQTEIFQDFVARIIQHECDHLQGYVFIDRVEST
RELITENEYHKLVLS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=164, Percent_Identity=34.7560975609756, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789682, Length=156, Percent_Identity=34.6153846153846, Blast_Score=87, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=32.1212121212121, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24645726, Length=163, Percent_Identity=30.0613496932515, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19633; Mature: 19502

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAMQREVLQLGNPKLRQTACPVQNPTDLEIQSLIDDLIGTMEESNGVGIAAPQVGIDLQ
CCHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCEE
VVIVASRPTLRYPHAPHMDPIAMINPHITPIGAERVKDWEGCLSVPGIRGLVPRASTISV
EEEEECCCCEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EYQDRNGQAQTEIFQDFVARIIQHECDHLQGYVFIDRVESTRELITENEYHKLVLS
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SAMQREVLQLGNPKLRQTACPVQNPTDLEIQSLIDDLIGTMEESNGVGIAAPQVGIDLQ
CHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCEE
VVIVASRPTLRYPHAPHMDPIAMINPHITPIGAERVKDWEGCLSVPGIRGLVPRASTISV
EEEEECCCCEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EYQDRNGQAQTEIFQDFVARIIQHECDHLQGYVFIDRVESTRELITENEYHKLVLS
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]