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The map label for this gene is 158336631

Identifier: 158336631

GI number: 158336631

Start: 3538348

End: 3540204

Strand: Direct

Name: 158336631

Synonym: AM1_3499

Alternate gene names: NA

Gene position: 3538348-3540204 (Clockwise)

Preceding gene: 158336630

Following gene: 158336632

Centisome position: 54.4

GC content: 51.32

Gene sequence:

>1857_bases
TTGCCCGGTACTGAGTATCTTGTTGGCGTGGGCAAAAGCGATATTACCGATCCAGCCATTGGCTTAGGCATGCAAGGGAT
GGCGGATAAGCATCAGCGTACGACGGGGGTTGAATCCCAACTCTATGCCAAAGTCTTTATTGTGGTTGATCCAGAGTCTG
CGAAGCGAGTTGCGATCGCATCCACCGATCTCTGGTCGGGCACCCATATCGTCAAGCATGAAGTCCTGCAGCGCCTCCAA
AAGATTTACGGTCCTGACCTGTACCAACTCGCTAATCTGCTCATTAGTGGAACCCATACCCACAGTGGACCGGGTGGTTA
TTCCCACTACTATCTGTACAACCATAGTTTGGGTGGACCCAACCAAATTGGCGGCTTTGATCGCCACAATTTTGAATGTA
TCGTCTCAGGGATTGTGGCAGCAATTCAGCAAGCCCATCACCAGCTAGCCCCCGGCAAAATCTACGTTAACCAAGGACAA
ATCGAGGATTGCGGCAGACAACGCTCACCCGCAGCCTACGAGCAAAATCCGGAAGCAGAACGCAAGCAGTACGGTCGCGA
CACGGACAATGAAATGCTGCTGCTGAAATTTGTCCATGTGAACGGTACCACCGAGACACCCATTGGCCTCTTGAACTGGT
ACGCCATCCATCCCACGGATCGCGGACAATACAACACCCTAGTAACGGGAGATAACAAAGGATATGCGGCAGAGCAGTTT
GGTGAATCCATTGTTGCAGCCTTTGCCAATGCCAACTGTGGTGATGTCTCTGGTAATGTCGCCTATGGCTCTATCCCAAC
GGGCATCAACGATAGCGAGCGAATGCAAAAACATGGCCAGCAGCAATATCAGCAGGCTCAAGTTTTATTCGACCAGGCCA
TTGAACCCCTGCAGGGCAGTATCGACTATCGACATACATTTATTGATATGTCCCATATTGAGATTGCCGACCAGCCAGGG
AAACGAACCTGGCCCGCAGCGTTAGGACTCTCCTTTGCTGCTGGCAGTAGTGAAGATAGCGATCCACCCTTTCAATTTAG
AGAAGGGATCACCGTCAGAACGATTACCAAGGCAGAGGCCTCGATTTTAGCGGCTTCCGGCGTGATTGCTGCGCTTGAGA
TGCCCGGTGCTGACCTCCCTGGCCACTTGGACCGCAAGCTCACCCGAGATTTTGTTGACGGCCATGCTCCCAAACCCATT
GCCTTTGCCCCTGGTTTAGCCAATCCCGACACGGACTACTACCCCATTACGCCAAAAGGAGTTCCCATTCAAATCCTTAA
AATTGGCAACCTAGTTTTAACAGGTATCCCTGGTGAAATCACCACCATGGCTGGCCGTCGCTTACGGGCAACGGTCCAGG
CTGAGCTGAAAGCAGACTATCTTGCCCTTGCCACCTATGCTAATGCTTTCTCCCAATACATCACAACGTTTGAAGAGTAC
CAGGCCCAGCACTACGAAGGTGCATCAACCTTGTATGGGCCATATACGTTGATGGCTTATCAGCAAGAGTTTCGCAAGTT
GGCGTTAGCCATGAAGCGGGGACGACCTATCTCGCTGGGTTCAGTACCTCCGGCTCCGGATCCTCAAATGATGTTGCCGA
GAGTGACCTTTAGAAACTTAACCAAAGTACTCCTCACTCCAACCTTTCATCAGAACGAAACTTCCCCACCACTCAAACTG
TCTTCCTTGCAGTTGCCCCCAGAAACGGACGTAGCTTGGATGATGCCCAAAGACTATCGAGAGACGGGGTTAGTGGTGAG
ACTTGATGGGGCCAACGTCAAAATTGCCGTCAATTCCCTGGTGACGATTCATGCCGACCACAGCATTGAAGTATCGCCTT
ATCATCCCTTGACTTAA

Upstream 100 bases:

>100_bases
AGTCAACCTCTCTAGACGTCAGTTTCTGAGTGCAGCCAGTATGGCAATGGGCTCAATGATTCTCGATCAACGTTTTGGAG
ATCCGGCTCAAGCGGTTGAG

Downstream 100 bases:

>100_bases
TGTTTCGGCCCGGCTTCATTCAGCCCAGACTCAACCTCTCATTTCTCCCTCATCAAACTTTTAAGTCAGGCTAATAGCGG
CTTTATCAGTCATTTCTACA

Product: neutral ceramidase

Products: NA

Alternate protein names: N-CDase; NCDase; Acylsphingosine deacylase; N-acylsphingosine amidohydrolase [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MPGTEYLVGVGKSDITDPAIGLGMQGMADKHQRTTGVESQLYAKVFIVVDPESAKRVAIASTDLWSGTHIVKHEVLQRLQ
KIYGPDLYQLANLLISGTHTHSGPGGYSHYYLYNHSLGGPNQIGGFDRHNFECIVSGIVAAIQQAHHQLAPGKIYVNQGQ
IEDCGRQRSPAAYEQNPEAERKQYGRDTDNEMLLLKFVHVNGTTETPIGLLNWYAIHPTDRGQYNTLVTGDNKGYAAEQF
GESIVAAFANANCGDVSGNVAYGSIPTGINDSERMQKHGQQQYQQAQVLFDQAIEPLQGSIDYRHTFIDMSHIEIADQPG
KRTWPAALGLSFAAGSSEDSDPPFQFREGITVRTITKAEASILAASGVIAALEMPGADLPGHLDRKLTRDFVDGHAPKPI
AFAPGLANPDTDYYPITPKGVPIQILKIGNLVLTGIPGEITTMAGRRLRATVQAELKADYLALATYANAFSQYITTFEEY
QAQHYEGASTLYGPYTLMAYQQEFRKLALAMKRGRPISLGSVPPAPDPQMMLPRVTFRNLTKVLLTPTFHQNETSPPLKL
SSLQLPPETDVAWMMPKDYRETGLVVRLDGANVKIAVNSLVTIHADHSIEVSPYHPLT

Sequences:

>Translated_618_residues
MPGTEYLVGVGKSDITDPAIGLGMQGMADKHQRTTGVESQLYAKVFIVVDPESAKRVAIASTDLWSGTHIVKHEVLQRLQ
KIYGPDLYQLANLLISGTHTHSGPGGYSHYYLYNHSLGGPNQIGGFDRHNFECIVSGIVAAIQQAHHQLAPGKIYVNQGQ
IEDCGRQRSPAAYEQNPEAERKQYGRDTDNEMLLLKFVHVNGTTETPIGLLNWYAIHPTDRGQYNTLVTGDNKGYAAEQF
GESIVAAFANANCGDVSGNVAYGSIPTGINDSERMQKHGQQQYQQAQVLFDQAIEPLQGSIDYRHTFIDMSHIEIADQPG
KRTWPAALGLSFAAGSSEDSDPPFQFREGITVRTITKAEASILAASGVIAALEMPGADLPGHLDRKLTRDFVDGHAPKPI
AFAPGLANPDTDYYPITPKGVPIQILKIGNLVLTGIPGEITTMAGRRLRATVQAELKADYLALATYANAFSQYITTFEEY
QAQHYEGASTLYGPYTLMAYQQEFRKLALAMKRGRPISLGSVPPAPDPQMMLPRVTFRNLTKVLLTPTFHQNETSPPLKL
SSLQLPPETDVAWMMPKDYRETGLVVRLDGANVKIAVNSLVTIHADHSIEVSPYHPLT
>Mature_617_residues
PGTEYLVGVGKSDITDPAIGLGMQGMADKHQRTTGVESQLYAKVFIVVDPESAKRVAIASTDLWSGTHIVKHEVLQRLQK
IYGPDLYQLANLLISGTHTHSGPGGYSHYYLYNHSLGGPNQIGGFDRHNFECIVSGIVAAIQQAHHQLAPGKIYVNQGQI
EDCGRQRSPAAYEQNPEAERKQYGRDTDNEMLLLKFVHVNGTTETPIGLLNWYAIHPTDRGQYNTLVTGDNKGYAAEQFG
ESIVAAFANANCGDVSGNVAYGSIPTGINDSERMQKHGQQQYQQAQVLFDQAIEPLQGSIDYRHTFIDMSHIEIADQPGK
RTWPAALGLSFAAGSSEDSDPPFQFREGITVRTITKAEASILAASGVIAALEMPGADLPGHLDRKLTRDFVDGHAPKPIA
FAPGLANPDTDYYPITPKGVPIQILKIGNLVLTGIPGEITTMAGRRLRATVQAELKADYLALATYANAFSQYITTFEEYQ
AQHYEGASTLYGPYTLMAYQQEFRKLALAMKRGRPISLGSVPPAPDPQMMLPRVTFRNLTKVLLTPTFHQNETSPPLKLS
SLQLPPETDVAWMMPKDYRETGLVVRLDGANVKIAVNSLVTIHADHSIEVSPYHPLT

Specific function: Hydrolyzes the ceramides into sphingosine and free fatty acid at an optimal pH of 8-9 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral ceramidase family [H]

Homologues:

Organism=Homo sapiens, GI221218981, Length=576, Percent_Identity=34.2013888888889, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI221307475, Length=563, Percent_Identity=33.9253996447602, Blast_Score=286, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24651405, Length=578, Percent_Identity=32.6989619377163, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24651403, Length=578, Percent_Identity=32.6989619377163, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24651401, Length=578, Percent_Identity=32.6989619377163, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24651397, Length=578, Percent_Identity=32.6989619377163, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24651399, Length=578, Percent_Identity=32.6989619377163, Blast_Score=264, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006823 [H]

Pfam domain/function: NA

EC number: =3.5.1.23 [H]

Molecular weight: Translated: 67663; Mature: 67532

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTEYLVGVGKSDITDPAIGLGMQGMADKHQRTTGVESQLYAKVFIVVDPESAKRVAIA
CCCCCEEEECCCCCCCCCHHCCCCCCCCHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEE
STDLWSGTHIVKHEVLQRLQKIYGPDLYQLANLLISGTHTHSGPGGYSHYYLYNHSLGGP
ECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCC
NQIGGFDRHNFECIVSGIVAAIQQAHHQLAPGKIYVNQGQIEDCGRQRSPAAYEQNPEAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCCCHH
RKQYGRDTDNEMLLLKFVHVNGTTETPIGLLNWYAIHPTDRGQYNTLVTGDNKGYAAEQF
HHHCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCHHHH
GESIVAAFANANCGDVSGNVAYGSIPTGINDSERMQKHGQQQYQQAQVLFDQAIEPLQGS
HHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDYRHTFIDMSHIEIADQPGKRTWPAALGLSFAAGSSEDSDPPFQFREGITVRTITKAEA
CCEEEEEEEEEEEEECCCCCCCCCHHHHCEEEECCCCCCCCCCHHHHCCCEEEEEEHHHH
SILAASGVIAALEMPGADLPGHLDRKLTRDFVDGHAPKPIAFAPGLANPDTDYYPITPKG
HHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCCCC
VPIQILKIGNLVLTGIPGEITTMAGRRLRATVQAELKADYLALATYANAFSQYITTFEEY
CCEEEEEECCEEEECCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAQHYEGASTLYGPYTLMAYQQEFRKLALAMKRGRPISLGSVPPAPDPQMMLPRVTFRNL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHH
TKVLLTPTFHQNETSPPLKLSSLQLPPETDVAWMMPKDYRETGLVVRLDGANVKIAVNSL
HHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCHHHCCCEEEECCCCEEEEEEEE
VTIHADHSIEVSPYHPLT
EEEECCCEEEECCCCCCC
>Mature Secondary Structure 
PGTEYLVGVGKSDITDPAIGLGMQGMADKHQRTTGVESQLYAKVFIVVDPESAKRVAIA
CCCCEEEECCCCCCCCCHHCCCCCCCCHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEE
STDLWSGTHIVKHEVLQRLQKIYGPDLYQLANLLISGTHTHSGPGGYSHYYLYNHSLGGP
ECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCC
NQIGGFDRHNFECIVSGIVAAIQQAHHQLAPGKIYVNQGQIEDCGRQRSPAAYEQNPEAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCCCHH
RKQYGRDTDNEMLLLKFVHVNGTTETPIGLLNWYAIHPTDRGQYNTLVTGDNKGYAAEQF
HHHCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCHHHH
GESIVAAFANANCGDVSGNVAYGSIPTGINDSERMQKHGQQQYQQAQVLFDQAIEPLQGS
HHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDYRHTFIDMSHIEIADQPGKRTWPAALGLSFAAGSSEDSDPPFQFREGITVRTITKAEA
CCEEEEEEEEEEEEECCCCCCCCCHHHHCEEEECCCCCCCCCCHHHHCCCEEEEEEHHHH
SILAASGVIAALEMPGADLPGHLDRKLTRDFVDGHAPKPIAFAPGLANPDTDYYPITPKG
HHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCCCC
VPIQILKIGNLVLTGIPGEITTMAGRRLRATVQAELKADYLALATYANAFSQYITTFEEY
CCEEEEEECCEEEECCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAQHYEGASTLYGPYTLMAYQQEFRKLALAMKRGRPISLGSVPPAPDPQMMLPRVTFRNL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHH
TKVLLTPTFHQNETSPPLKLSSLQLPPETDVAWMMPKDYRETGLVVRLDGANVKIAVNSL
HHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCHHHCCCEEEECCCCEEEEEEEE
VTIHADHSIEVSPYHPLT
EEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10593963; 10984043 [H]