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The map label for this gene is tig [H]

Identifier: 158336584

GI number: 158336584

Start: 3477341

End: 3478987

Strand: Direct

Name: tig [H]

Synonym: AM1_3448

Alternate gene names: 158336584

Gene position: 3477341-3478987 (Clockwise)

Preceding gene: 158336581

Following gene: 158336585

Centisome position: 53.47

GC content: 44.51

Gene sequence:

>1647_bases
TTGTCTAAAACTAAAGAGCTACTAAAGGTAACACAGGAAAAGCTCCCTGGCAGTCAAATCAGTCTGGAAATAGAAATTTC
TCCTGAGCAATCGCGTCAAGCGTATGAGCAAGTAATTATTAAGTTCATGCGATCGGCTAATATTCCTGGTTTTCGTAAAG
GTAAAGTCCCCAAACAGGTTATCCTCCAACGGTTTGGCACAGATCAGTTAAAAGCCTCTGCGTTGGAAGATTTGGTTCAA
AAGAATTTTGAAGCTGCGGTTAAGCAAGAAGAGATTGAAGCCTTAGGTAACGTTCAATTCCGCTCATCTTTTGATGAACT
CGCTGCTGAGTTTGAACCCGGTAAGGCGATTGTATTTGCCGTTAGTGTCGATGTCCCCCCAGAAGTCACCCTTAAGAAGT
ATCAAGACTTTAAAGTTACGGCTGAAAAGATAGAGTATGAAGCAGCTAAGGTCGATGAAGTTCTAGAAGAACATCGATCT
TCGAATGCAACCTTGGTCCCTGTCGAGGATCGAGCTGCAGCAGCGAATGATGTTACCCTCGTCGATTTTGAAAGCCGATT
CTTACCCAGTGAGTCAGATGCTGATGAAGAAGGTGAGACGCCAGAGGGAGAGTCCGTTCAAGACTTCCAACTGGAACTCG
TGAAAGGGAAGTTTATTGACGATTTGATCGATGGCATCATCGGTATGCAAATTGGGGAAACCAAGAATATTGCAGTGACA
CTGCCTCAAGAATATTTCCAAGATGAGTTGGCTGGCCGCTCTGCTGAATTTACCGTCAGTCTCAAGGATATTAAAGCGAA
AGAGCTGCCACCTCTAGATGATGATTTTGCCCAAGATGTTAGCGAATTTTCGACCTTGGCAGAATTGCGCCAGTTCTTAG
AAGAGCAATATCAGCAAGAAGCCCAAGATAGTACGGATGCCAATGTGGAAGCAGCTCTTCTAGAGGCATTGATTGAAGAG
CTAGACGTTGATCTACCTGAAAGCATGATCGACGATGAGCTGAATGTCATGATTCGAGAAACTCTCAGTCGCTTGCAGTC
TAATGGTTTAGACATTAATAAGCTGGTGACCAAAGAGATGCTCGGGGAGATGAAAGAACGCATGCGATCAGATGCAATTC
CTCGTTTGAAGCGAACTCTTGCCTTGGCCGAAGTGGCGAAAGCGGAATCGATCACAGTTGAGGCGGATGCTATTGCAGAT
CGATGCCAAGAAATTCTAAAGGATCTGCAAGGACAAGATATTGATCGGGATCGCCTAGAAGCTGTTGTTGAAGATGAGTT
GTTACATAAGCAGGTGGTAGCTTGGCTAACCGAACACTCTGAGGTGGAATTGGTTGAGCCAAAACCTGAAGAGGATGCTG
CAGAAGCGGAGGCTAAAGCAGAGGAAGAGGCTACTGCTAAAGAAGTAAAGCCATCTGAAGCTAAGAAATCGGCTAAATCA
ACCGCGAAAAAAGCATCTACAAAAAAAGCGAAGTCGGCTAAAGTAGCTGAGGAAGTGGCTGATGAGACTAAGGAAGAATC
AGCTGCCAAGAAAACACCTGCTAAAGCTAAACGGTCAACGGCAAAAACCACTAAGAAAACATCTACTAAGACGAAAAAAA
CATCTGCTAAGCCCAAAGCAGATGATTCAGCCACTGATTCTGAATAA

Upstream 100 bases:

>100_bases
TTGAATTAGAATAGCTCTACTAATATCAATCAACCCTGCTTGATTAGTTGCATATTATTACTCACACATTATTTTTGGTC
CGCTGGGAGAGGGAATCGAT

Downstream 100 bases:

>100_bases
TAGGTTGCTAGTCCGTTTAAGAACGGGAGCAGCTTTTTCCAAAGGAGTTAAGGCATAATATAACTGGTTGACAAGGTGAA
AGGGTAATCGCCTTAGATCC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSKTKELLKVTQEKLPGSQISLEIEISPEQSRQAYEQVIIKFMRSANIPGFRKGKVPKQVILQRFGTDQLKASALEDLVQ
KNFEAAVKQEEIEALGNVQFRSSFDELAAEFEPGKAIVFAVSVDVPPEVTLKKYQDFKVTAEKIEYEAAKVDEVLEEHRS
SNATLVPVEDRAAAANDVTLVDFESRFLPSESDADEEGETPEGESVQDFQLELVKGKFIDDLIDGIIGMQIGETKNIAVT
LPQEYFQDELAGRSAEFTVSLKDIKAKELPPLDDDFAQDVSEFSTLAELRQFLEEQYQQEAQDSTDANVEAALLEALIEE
LDVDLPESMIDDELNVMIRETLSRLQSNGLDINKLVTKEMLGEMKERMRSDAIPRLKRTLALAEVAKAESITVEADAIAD
RCQEILKDLQGQDIDRDRLEAVVEDELLHKQVVAWLTEHSEVELVEPKPEEDAAEAEAKAEEEATAKEVKPSEAKKSAKS
TAKKASTKKAKSAKVAEEVADETKEESAAKKTPAKAKRSTAKTTKKTSTKTKKTSAKPKADDSATDSE

Sequences:

>Translated_548_residues
MSKTKELLKVTQEKLPGSQISLEIEISPEQSRQAYEQVIIKFMRSANIPGFRKGKVPKQVILQRFGTDQLKASALEDLVQ
KNFEAAVKQEEIEALGNVQFRSSFDELAAEFEPGKAIVFAVSVDVPPEVTLKKYQDFKVTAEKIEYEAAKVDEVLEEHRS
SNATLVPVEDRAAAANDVTLVDFESRFLPSESDADEEGETPEGESVQDFQLELVKGKFIDDLIDGIIGMQIGETKNIAVT
LPQEYFQDELAGRSAEFTVSLKDIKAKELPPLDDDFAQDVSEFSTLAELRQFLEEQYQQEAQDSTDANVEAALLEALIEE
LDVDLPESMIDDELNVMIRETLSRLQSNGLDINKLVTKEMLGEMKERMRSDAIPRLKRTLALAEVAKAESITVEADAIAD
RCQEILKDLQGQDIDRDRLEAVVEDELLHKQVVAWLTEHSEVELVEPKPEEDAAEAEAKAEEEATAKEVKPSEAKKSAKS
TAKKASTKKAKSAKVAEEVADETKEESAAKKTPAKAKRSTAKTTKKTSTKTKKTSAKPKADDSATDSE
>Mature_547_residues
SKTKELLKVTQEKLPGSQISLEIEISPEQSRQAYEQVIIKFMRSANIPGFRKGKVPKQVILQRFGTDQLKASALEDLVQK
NFEAAVKQEEIEALGNVQFRSSFDELAAEFEPGKAIVFAVSVDVPPEVTLKKYQDFKVTAEKIEYEAAKVDEVLEEHRSS
NATLVPVEDRAAAANDVTLVDFESRFLPSESDADEEGETPEGESVQDFQLELVKGKFIDDLIDGIIGMQIGETKNIAVTL
PQEYFQDELAGRSAEFTVSLKDIKAKELPPLDDDFAQDVSEFSTLAELRQFLEEQYQQEAQDSTDANVEAALLEALIEEL
DVDLPESMIDDELNVMIRETLSRLQSNGLDINKLVTKEMLGEMKERMRSDAIPRLKRTLALAEVAKAESITVEADAIADR
CQEILKDLQGQDIDRDRLEAVVEDELLHKQVVAWLTEHSEVELVEPKPEEDAAEAEAKAEEEATAKEVKPSEAKKSAKST
AKKASTKKAKSAKVAEEVADETKEESAAKKTPAKAKRSTAKTTKKTSTKTKKTSAKPKADDSATDSE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=342, Percent_Identity=28.9473684210526, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 60770; Mature: 60638

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTKELLKVTQEKLPGSQISLEIEISPEQSRQAYEQVIIKFMRSANIPGFRKGKVPKQV
CCHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ILQRFGTDQLKASALEDLVQKNFEAAVKQEEIEALGNVQFRSSFDELAAEFEPGKAIVFA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
VSVDVPPEVTLKKYQDFKVTAEKIEYEAAKVDEVLEEHRSSNATLVPVEDRAAAANDVTL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEE
VDFESRFLPSESDADEEGETPEGESVQDFQLELVKGKFIDDLIDGIIGMQIGETKNIAVT
EECHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LPQEYFQDELAGRSAEFTVSLKDIKAKELPPLDDDFAQDVSEFSTLAELRQFLEEQYQQE
CCHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQDSTDANVEAALLEALIEELDVDLPESMIDDELNVMIRETLSRLQSNGLDINKLVTKEM
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LGEMKERMRSDAIPRLKRTLALAEVAKAESITVEADAIADRCQEILKDLQGQDIDRDRLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHH
AVVEDELLHKQVVAWLTEHSEVELVEPKPEEDAAEAEAKAEEEATAKEVKPSEAKKSAKS
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TAKKASTKKAKSAKVAEEVADETKEESAAKKTPAKAKRSTAKTTKKTSTKTKKTSAKPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCC
DDSATDSE
CCCCCCCC
>Mature Secondary Structure 
SKTKELLKVTQEKLPGSQISLEIEISPEQSRQAYEQVIIKFMRSANIPGFRKGKVPKQV
CHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ILQRFGTDQLKASALEDLVQKNFEAAVKQEEIEALGNVQFRSSFDELAAEFEPGKAIVFA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
VSVDVPPEVTLKKYQDFKVTAEKIEYEAAKVDEVLEEHRSSNATLVPVEDRAAAANDVTL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEE
VDFESRFLPSESDADEEGETPEGESVQDFQLELVKGKFIDDLIDGIIGMQIGETKNIAVT
EECHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LPQEYFQDELAGRSAEFTVSLKDIKAKELPPLDDDFAQDVSEFSTLAELRQFLEEQYQQE
CCHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQDSTDANVEAALLEALIEELDVDLPESMIDDELNVMIRETLSRLQSNGLDINKLVTKEM
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LGEMKERMRSDAIPRLKRTLALAEVAKAESITVEADAIADRCQEILKDLQGQDIDRDRLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHH
AVVEDELLHKQVVAWLTEHSEVELVEPKPEEDAAEAEAKAEEEATAKEVKPSEAKKSAKS
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TAKKASTKKAKSAKVAEEVADETKEESAAKKTPAKAKRSTAKTTKKTSTKTKKTSAKPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCC
DDSATDSE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA