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The map label for this gene is tsf

Identifier: 158336563

GI number: 158336563

Start: 3457545

End: 3458264

Strand: Reverse

Name: tsf

Synonym: AM1_3427

Alternate gene names: 158336563

Gene position: 3458264-3457545 (Counterclockwise)

Preceding gene: 158336564

Following gene: 158336562

Centisome position: 53.17

GC content: 45.56

Gene sequence:

>720_bases
ATGGCAGCAATATCAGCAAAAGATGTTAAAGAACTGCGTGATAAAACTGGCGCAGGGATGATGGACTGCAAGAAAGCCTT
GCAAGAAAATGATGGCGATCAAGAAAAAGCCATTGCCTATCTGCGCAAAAAAGGCCTCTCCCAGGCGGGTAAAAAGTCCG
GTCGCGTCACCGCTGAAGGACTCGTTGATAGCTACATCCACTTCGGTGGTCAAATTGGTGTGTTGGTCGAAGTCAACTGT
GAAACAGATTTTGTAGCCCGCAACGAAGCCTTTAAAGAGCTGGTACAAGACATTGCCAAGCAAATCGCCGCTTGCCCCAA
TGTCCAGTATGTTGATACCGATGAAATCCCTCAGGATTTTGTGGAAAAAGAGAAGGCAGTGGCCATGGGCTCTGACGCCC
TTAAGGGTAAGCCCGACAATATTAAAGAAAAAATTGTCCAAGGCAAGTTAGATAAGACCTTAAAAGAACTATGTCTCTTG
CATCAACCCTATATCAAAGACCAAAGCATCACAGTCCAAGAACTGCTGCAGCAGGCTATTTCTAAGCTGGGCGAGAACAT
GAAGGTTCGTCGCTTTACTCGCTTTGTCCTAGGCGAAGGCATTGATAAAGTTGAGTCTAACTTAGCCGAAGAAGTGGCAG
CCCAAACTAAGCCTAAAGCAGAAGAGAAACCTGCGGCCAAAAAGGCTACTTCTAAGAAGAAAAAAGGTAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
CCTGCTGGACATCTAAGGGACGCCTACTGTTCGCTCTTAGATGTCCCTTAACGTTTTAGCTTCAAGATAAACACCATCAT
AGATACACCGAGAAAAGACC

Downstream 100 bases:

>100_bases
ACAAACTAAAAAGGTAGACCAAGGTCTACCTTTTTAGTTTAAGCGATTATAGCGTGCTGCCCGCCGTTCCATTTTTGAGG
CTGCTACCATCGTAAAAAAG

Product: translation elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MAAISAKDVKELRDKTGAGMMDCKKALQENDGDQEKAIAYLRKKGLSQAGKKSGRVTAEGLVDSYIHFGGQIGVLVEVNC
ETDFVARNEAFKELVQDIAKQIAACPNVQYVDTDEIPQDFVEKEKAVAMGSDALKGKPDNIKEKIVQGKLDKTLKELCLL
HQPYIKDQSITVQELLQQAISKLGENMKVRRFTRFVLGEGIDKVESNLAEEVAAQTKPKAEEKPAAKKATSKKKKGKKK

Sequences:

>Translated_239_residues
MAAISAKDVKELRDKTGAGMMDCKKALQENDGDQEKAIAYLRKKGLSQAGKKSGRVTAEGLVDSYIHFGGQIGVLVEVNC
ETDFVARNEAFKELVQDIAKQIAACPNVQYVDTDEIPQDFVEKEKAVAMGSDALKGKPDNIKEKIVQGKLDKTLKELCLL
HQPYIKDQSITVQELLQQAISKLGENMKVRRFTRFVLGEGIDKVESNLAEEVAAQTKPKAEEKPAAKKATSKKKKGKKK
>Mature_238_residues
AAISAKDVKELRDKTGAGMMDCKKALQENDGDQEKAIAYLRKKGLSQAGKKSGRVTAEGLVDSYIHFGGQIGVLVEVNCE
TDFVARNEAFKELVQDIAKQIAACPNVQYVDTDEIPQDFVEKEKAVAMGSDALKGKPDNIKEKIVQGKLDKTLKELCLLH
QPYIKDQSITVQELLQQAISKLGENMKVRRFTRFVLGEGIDKVESNLAEEVAAQTKPKAEEKPAAKKATSKKKKGKKK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI291084500, Length=195, Percent_Identity=34.8717948717949, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI171846268, Length=195, Percent_Identity=35.3846153846154, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI291084498, Length=103, Percent_Identity=46.6019417475728, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI291084502, Length=103, Percent_Identity=46.6019417475728, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786366, Length=92, Percent_Identity=54.3478260869565, Blast_Score=100, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17561440, Length=202, Percent_Identity=31.6831683168317, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921466, Length=128, Percent_Identity=38.28125, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_ACAM1 (B0C074)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517737.1
- ProteinModelPortal:   B0C074
- SMR:   B0C074
- GeneID:   5682235
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3427
- HOGENOM:   HBG713289
- OMA:   MVELSCE
- BioCyc:   AMAR329726:AM1_3427-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 26322; Mature: 26190

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAISAKDVKELRDKTGAGMMDCKKALQENDGDQEKAIAYLRKKGLSQAGKKSGRVTAEG
CCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
LVDSYIHFGGQIGVLVEVNCETDFVARNEAFKELVQDIAKQIAACPNVQYVDTDEIPQDF
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHH
VEKEKAVAMGSDALKGKPDNIKEKIVQGKLDKTLKELCLLHQPYIKDQSITVQELLQQAI
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SKLGENMKVRRFTRFVLGEGIDKVESNLAEEVAAQTKPKAEEKPAAKKATSKKKKGKKK
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AAISAKDVKELRDKTGAGMMDCKKALQENDGDQEKAIAYLRKKGLSQAGKKSGRVTAEG
CCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
LVDSYIHFGGQIGVLVEVNCETDFVARNEAFKELVQDIAKQIAACPNVQYVDTDEIPQDF
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHH
VEKEKAVAMGSDALKGKPDNIKEKIVQGKLDKTLKELCLLHQPYIKDQSITVQELLQQAI
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SKLGENMKVRRFTRFVLGEGIDKVESNLAEEVAAQTKPKAEEKPAAKKATSKKKKGKKK
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA