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The map label for this gene is tcmP [H]

Identifier: 158336510

GI number: 158336510

Start: 3405104

End: 3405970

Strand: Direct

Name: tcmP [H]

Synonym: AM1_3374

Alternate gene names: 158336510

Gene position: 3405104-3405970 (Clockwise)

Preceding gene: 158336509

Following gene: 158336514

Centisome position: 52.36

GC content: 43.71

Gene sequence:

>867_bases
ATGCCATCTACCCAATCTGTAAGCGCAACACAATTCCGAGGTGTTCCTGAAACATTGATGATCACCCTCTATGCTCGCGC
TATGGAACACCAGCGGGTTAACCCTATCCTTCGCGATGCCAAAGCAGCAGACATTATTCAACAGCTCGATTACGATTTTT
CTAAGTTCGAGCAAGGCTGGTCATCGCAATTAGGCTGTGTGATTCGAGCAAGACAGATAGATCAGATTGTCCAAAACTTT
ATCAAAACCCATCCTTATGCTGTGATTGTCAATTTAGGGGCAGGTTTGTGTACACGATTCTCACGATTACAAACGGCTCA
TATCCATTGGTATGACGTTGATTTTCCTGATGTTATTGATCTACGCAAACAGGTATTTGCCGCCTGTGGGGGCCAAGACG
GAAGCGATCACAATCATTTAGTTGCCAAATCTATCTTGGACTTTACCTGGATGGATGAGATTGACCGTGAGCTAAAGCAA
CCGATGATGGTGATTTATGAAGGGGTGTCTATGTATCTTACAGAAGCGGATAATAAAGCTCTCTTACAGCAGATTCAAGC
TAGATTTGGCCCTATTGAAGTGTTATTTGATGTGATTAGTCAACGAACTGCCAGAAGTACTGCAAAACATGACACGGTTT
CAAAGACTAGTGCTGAGTTTAAGTGGGGCATCAATCAGTCCCTAGATCTAGAACATTGGGGGTTAGGCTTTATCCTCAAG
CGGGAGGAGTTTTATCTACAGCATTTTTTGGATGATTTACAGCGATTACCAGCCGTTTGGCGTTTGATCAGTCGAGTTTT
TCCGGCTTTACCCATGATGTTATTCAAAAATTCAGGACGGATTGTGGGACTCCAAGTTGGGCTGTAG

Upstream 100 bases:

>100_bases
ATTTCTAACCCAGTATGTAACGCAGTGTCTATCTGTGTATCATCAATCGTTTTTAGTTCATTTCTTCTGTGGACGAGAGG
CACCCAAACTGGAGGTTTAT

Downstream 100 bases:

>100_bases
TAACTACATTGGACGAGTGGTGACGATGGCGTTACTTTGGTTTTATTTATGAAGACTCTCATGCTCCCATAACCAAAGTT
AAGCACCTAGCTAAAATTAA

Product: tetracenomycin polyketide synthesis O-methyltransferase TcmP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPSTQSVSATQFRGVPETLMITLYARAMEHQRVNPILRDAKAADIIQQLDYDFSKFEQGWSSQLGCVIRARQIDQIVQNF
IKTHPYAVIVNLGAGLCTRFSRLQTAHIHWYDVDFPDVIDLRKQVFAACGGQDGSDHNHLVAKSILDFTWMDEIDRELKQ
PMMVIYEGVSMYLTEADNKALLQQIQARFGPIEVLFDVISQRTARSTAKHDTVSKTSAEFKWGINQSLDLEHWGLGFILK
REEFYLQHFLDDLQRLPAVWRLISRVFPALPMMLFKNSGRIVGLQVGL

Sequences:

>Translated_288_residues
MPSTQSVSATQFRGVPETLMITLYARAMEHQRVNPILRDAKAADIIQQLDYDFSKFEQGWSSQLGCVIRARQIDQIVQNF
IKTHPYAVIVNLGAGLCTRFSRLQTAHIHWYDVDFPDVIDLRKQVFAACGGQDGSDHNHLVAKSILDFTWMDEIDRELKQ
PMMVIYEGVSMYLTEADNKALLQQIQARFGPIEVLFDVISQRTARSTAKHDTVSKTSAEFKWGINQSLDLEHWGLGFILK
REEFYLQHFLDDLQRLPAVWRLISRVFPALPMMLFKNSGRIVGLQVGL
>Mature_287_residues
PSTQSVSATQFRGVPETLMITLYARAMEHQRVNPILRDAKAADIIQQLDYDFSKFEQGWSSQLGCVIRARQIDQIVQNFI
KTHPYAVIVNLGAGLCTRFSRLQTAHIHWYDVDFPDVIDLRKQVFAACGGQDGSDHNHLVAKSILDFTWMDEIDRELKQP
MMVIYEGVSMYLTEADNKALLQQIQARFGPIEVLFDVISQRTARSTAKHDTVSKTSAEFKWGINQSLDLEHWGLGFILKR
EEFYLQHFLDDLQRLPAVWRLISRVFPALPMMLFKNSGRIVGLQVGL

Specific function: O-methyltransferase that catalyzes the methylation of the C-9 carboxy group of tetracenomycin E (TCM E) to yield TCM A2. Catalyzes as well the following side reactions:methylation of 8- O-methyl-TCM D3 to 9-carboxymethyl-8-O-methyl-TCM D3; and of TCM B3 t

COG id: COG3315

COG function: function code Q; O-Methyltransferase involved in polyketide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007213
- InterPro:   IPR016874 [H]

Pfam domain/function: PF04072 LCM [H]

EC number: NA

Molecular weight: Translated: 32987; Mature: 32856

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTQSVSATQFRGVPETLMITLYARAMEHQRVNPILRDAKAADIIQQLDYDFSKFEQGW
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCH
SSQLGCVIRARQIDQIVQNFIKTHPYAVIVNLGAGLCTRFSRLQTAHIHWYDVDFPDVID
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCEEEEEEEECCCHHHHH
LRKQVFAACGGQDGSDHNHLVAKSILDFTWMDEIDRELKQPMMVIYEGVSMYLTEADNKA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHH
LLQQIQARFGPIEVLFDVISQRTARSTAKHDTVSKTSAEFKWGINQSLDLEHWGLGFILK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCEEEE
REEFYLQHFLDDLQRLPAVWRLISRVFPALPMMLFKNSGRIVGLQVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
PSTQSVSATQFRGVPETLMITLYARAMEHQRVNPILRDAKAADIIQQLDYDFSKFEQGW
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCH
SSQLGCVIRARQIDQIVQNFIKTHPYAVIVNLGAGLCTRFSRLQTAHIHWYDVDFPDVID
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCEEEEEEEECCCHHHHH
LRKQVFAACGGQDGSDHNHLVAKSILDFTWMDEIDRELKQPMMVIYEGVSMYLTEADNKA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHH
LLQQIQARFGPIEVLFDVISQRTARSTAKHDTVSKTSAEFKWGINQSLDLEHWGLGFILK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCEEEE
REEFYLQHFLDDLQRLPAVWRLISRVFPALPMMLFKNSGRIVGLQVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]