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The map label for this gene is tyrS [H]

Identifier: 158336483

GI number: 158336483

Start: 3379757

End: 3380971

Strand: Direct

Name: tyrS [H]

Synonym: AM1_3347

Alternate gene names: 158336483

Gene position: 3379757-3380971 (Clockwise)

Preceding gene: 158336477

Following gene: 158336487

Centisome position: 51.97

GC content: 48.23

Gene sequence:

>1215_bases
ATGGCAGATGCAGTGTCTTCGTTGCTCCAGAGGGGCGTTAGCGATATTTTCCCCAATAATGCTGACTCACAGCAAGAGGA
TGACAATTTAGCCCATCGCTTGGAAAACGCGGATCAACCGTTGCGCATCAAGCTGGGCATTGACCCCACGGGAACGGATC
TTCATTTAGGCCATAGTCTTCCTATTCGTAAACTACGGGCTTTTCAGGATTTAGGTCATAAAGCCGTTTTGATTATTGGC
GATTTTACGGCGCGGGTAGGTGATCCAACGGGCAAGTCTGAGGTTCGCCGTCAGCTGACAGAAGCAGAGGTGCAGCATAA
TATTCAGACCTACCTAGATCAGATTAAGCCGATTTTAGATTTTGAGACCCCCGATCGGTTAGAGGTTCGCTATAACTCTG
AATGGTTGTCCAAAATCGATTTGGCAAAGACCTTAGAACTTCTGACCACAATGACTGTGGGTCAGATGCTGGCAAAAGAA
GGATTTGCAGAGCGCTACCAGCAGGGAACACCAGTCTATTTGCATGAATTTTTATATCCTCTACTGCAGGGTTATGACTC
AGTGGCAGTCGAAGCGGATGTGGAGTTAGGAGGCACGGATCAGAAGTTTAATATTGCCATTGGTCGAGACTTGCAGCGAC
ATTTTGGCCTGCGCCCCCAGTTTGGCCTGCTAACCCCAATTCTGTTAGGGACGGATGGCGTACAAAAAATGTCCAAGTCT
TTGAATAACTATGTGGGCTTGGCTGAAGATGCAGTCAGCATGTACTCCAAGTTGGAGAAGACGCCGGATGCTCTGCTGCC
AGAGTACTTTGAGCACCTAACCAATTTGTCCCTCAAAGATTTGCCGGAGAATCCTCGCGAACAGCAGAAGCTGTTGGCCC
ATACTGTGGTGAGCCAGTATCACAGTGTTGCGATCGCAAATGAAACCCAACAGTCCCTAGCGGCAATGGTGACCCAGGGA
GATATGAGTCAAACCGATGTCATTCCTGAATATTCATTGGCAGAGGTGACCTTTCCAGCCAAGCTGTTCTATCTTCTGAG
TGCCAGTGGTTTGTGTTCCAGCAGTAGTGATGCCCGTCGCCAAATTAAGGGTGGAGCTGTGAAATTAGATGGCACTAAAA
TCACCGTGGTCGATCAAACCTACGACCAGCCAGACGATCTGATCGGGGCTGTTTTACAAGTGGGTAAAAAGAAGTTTATT
CGGTTTATCGCCTAA

Upstream 100 bases:

>100_bases
TTGCGCTGCAATGGCATACTAGAATTTGCTTTTCAGTCGCTGGAGCGATTGGCGGCGATATGGGCGATTGCCCTTTGTTC
ATCCTTACTATTTCTCACCT

Downstream 100 bases:

>100_bases
AACGGCATAGCTTTGGTCGTAGTTCGAGGGGGTATGGATGGGCTGAAGCGCTCGGGGGAGAGAATCATCAGGCTCAATGA
CCTGGGCAACTCACATGAGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MADAVSSLLQRGVSDIFPNNADSQQEDDNLAHRLENADQPLRIKLGIDPTGTDLHLGHSLPIRKLRAFQDLGHKAVLIIG
DFTARVGDPTGKSEVRRQLTEAEVQHNIQTYLDQIKPILDFETPDRLEVRYNSEWLSKIDLAKTLELLTTMTVGQMLAKE
GFAERYQQGTPVYLHEFLYPLLQGYDSVAVEADVELGGTDQKFNIAIGRDLQRHFGLRPQFGLLTPILLGTDGVQKMSKS
LNNYVGLAEDAVSMYSKLEKTPDALLPEYFEHLTNLSLKDLPENPREQQKLLAHTVVSQYHSVAIANETQQSLAAMVTQG
DMSQTDVIPEYSLAEVTFPAKLFYLLSASGLCSSSSDARRQIKGGAVKLDGTKITVVDQTYDQPDDLIGAVLQVGKKKFI
RFIA

Sequences:

>Translated_404_residues
MADAVSSLLQRGVSDIFPNNADSQQEDDNLAHRLENADQPLRIKLGIDPTGTDLHLGHSLPIRKLRAFQDLGHKAVLIIG
DFTARVGDPTGKSEVRRQLTEAEVQHNIQTYLDQIKPILDFETPDRLEVRYNSEWLSKIDLAKTLELLTTMTVGQMLAKE
GFAERYQQGTPVYLHEFLYPLLQGYDSVAVEADVELGGTDQKFNIAIGRDLQRHFGLRPQFGLLTPILLGTDGVQKMSKS
LNNYVGLAEDAVSMYSKLEKTPDALLPEYFEHLTNLSLKDLPENPREQQKLLAHTVVSQYHSVAIANETQQSLAAMVTQG
DMSQTDVIPEYSLAEVTFPAKLFYLLSASGLCSSSSDARRQIKGGAVKLDGTKITVVDQTYDQPDDLIGAVLQVGKKKFI
RFIA
>Mature_403_residues
ADAVSSLLQRGVSDIFPNNADSQQEDDNLAHRLENADQPLRIKLGIDPTGTDLHLGHSLPIRKLRAFQDLGHKAVLIIGD
FTARVGDPTGKSEVRRQLTEAEVQHNIQTYLDQIKPILDFETPDRLEVRYNSEWLSKIDLAKTLELLTTMTVGQMLAKEG
FAERYQQGTPVYLHEFLYPLLQGYDSVAVEADVELGGTDQKFNIAIGRDLQRHFGLRPQFGLLTPILLGTDGVQKMSKSL
NNYVGLAEDAVSMYSKLEKTPDALLPEYFEHLTNLSLKDLPENPREQQKLLAHTVVSQYHSVAIANETQQSLAAMVTQGD
MSQTDVIPEYSLAEVTFPAKLFYLLSASGLCSSSSDARRQIKGGAVKLDGTKITVVDQTYDQPDDLIGAVLQVGKKKFIR
FIA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=430, Percent_Identity=25.5813953488372, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787925, Length=406, Percent_Identity=28.8177339901478, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25145802, Length=242, Percent_Identity=28.099173553719, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325160, Length=416, Percent_Identity=26.4423076923077, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130383, Length=405, Percent_Identity=24.1975308641975, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44869; Mature: 44737

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAVSSLLQRGVSDIFPNNADSQQEDDNLAHRLENADQPLRIKLGIDPTGTDLHLGHSL
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCC
PIRKLRAFQDLGHKAVLIIGDFTARVGDPTGKSEVRRQLTEAEVQHNIQTYLDQIKPILD
CHHHHHHHHHCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FETPDRLEVRYNSEWLSKIDLAKTLELLTTMTVGQMLAKEGFAERYQQGTPVYLHEFLYP
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLQGYDSVAVEADVELGGTDQKFNIAIGRDLQRHFGLRPQFGLLTPILLGTDGVQKMSKS
HHCCCCCEEEEEEEEECCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHH
LNNYVGLAEDAVSMYSKLEKTPDALLPEYFEHLTNLSLKDLPENPREQQKLLAHTVVSQY
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHH
HSVAIANETQQSLAAMVTQGDMSQTDVIPEYSLAEVTFPAKLFYLLSASGLCSSSSDARR
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QIKGGAVKLDGTKITVVDQTYDQPDDLIGAVLQVGKKKFIRFIA
HHCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADAVSSLLQRGVSDIFPNNADSQQEDDNLAHRLENADQPLRIKLGIDPTGTDLHLGHSL
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCC
PIRKLRAFQDLGHKAVLIIGDFTARVGDPTGKSEVRRQLTEAEVQHNIQTYLDQIKPILD
CHHHHHHHHHCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FETPDRLEVRYNSEWLSKIDLAKTLELLTTMTVGQMLAKEGFAERYQQGTPVYLHEFLYP
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLQGYDSVAVEADVELGGTDQKFNIAIGRDLQRHFGLRPQFGLLTPILLGTDGVQKMSKS
HHCCCCCEEEEEEEEECCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHH
LNNYVGLAEDAVSMYSKLEKTPDALLPEYFEHLTNLSLKDLPENPREQQKLLAHTVVSQY
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHH
HSVAIANETQQSLAAMVTQGDMSQTDVIPEYSLAEVTFPAKLFYLLSASGLCSSSSDARR
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QIKGGAVKLDGTKITVVDQTYDQPDDLIGAVLQVGKKKFIRFIA
HHCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]