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The map label for this gene is pbpG [H]

Identifier: 158336481

GI number: 158336481

Start: 3377450

End: 3379384

Strand: Reverse

Name: pbpG [H]

Synonym: AM1_3345

Alternate gene names: 158336481

Gene position: 3379384-3377450 (Counterclockwise)

Preceding gene: 158336482

Following gene: 158336480

Centisome position: 51.96

GC content: 50.13

Gene sequence:

>1935_bases
GTGTCTGTATCACAAACTGCCAGCCCCAAAAACCCCAATCGCTTGTCCAAAGGCACCAGTTTTATTGGGGGGATTGGCAA
AGCAGCGGGTTCTACAATTCTGATCACGACGATGCTGGCTAGCGCCGCTGCAGCCGGTAGCTTAATGGGGGTCGCCATTA
GCTTTCGCAATTTACCGGACGTTCGGTCCTTAAAGGGTTATATTCCCAACGAGACCACCCATATTTACGATATAAACGGC
AAAATTCTGGCCAGCCTTCACGATGAAGAAAACCGTGAAGTCGTTCCTTTAGACAATATTTCACCCCATTTGAAGTTGGC
CGTGTTAGCCATTGAAGACAGCAACTTCTATAAACATAATGGGATTAATCCCTACGGTATCCTCCGGGCCACGGTCACGA
ATATACAGGCTGGAGAAACCGTTCAGGGTGCCTCTACCTTAACCATGCAGCTGGTCAAAAACCTGTTTTTGACCCCTAGG
CGAGAACTGGGTCGGAAGGTTGCAGAGGCGGTCCTAGCCATGCGGCTGGAGAAGCTATTCAAGAAAGATGAAATTTTAGA
AATGTACCTCAATCAAGTGTATTGGGGACATAACACCTATGGCGTTGAGACCGCCGCCCGCAGCTATTTCAATAAATCAT
CATCGGATCTGAACCTCGCAGAATCAGCCATGATGGCGGGTATTATTCAAGCCCCTGAAAGCTTTAGCCCCTTTGATTTT
GACTCCGTCACCGATGAAACCCTGGAGATTGCCAAACGGAGGCAAGCGGTGGTTCTCAATCGGATGAAAAAGCTGACCTG
GATTACCGAAGAAGAAGAGCAGGCTGCTTTAGACACTCCTTTAAAGTTTGGTAAGGTCACCTCCTTCCAACTCAGTCGCT
CGCCCTACATCACCAATACCGTGATTAAGGAACTGACCCGTCGGTTTGGCCGCGATGCCCTCAGTAAAGGGGGCATGCGA
ATTCAAACCACCATTGACATCAAGATGCAGGAAATTGCCGAAGATTCGGTGAAATGGGGACAACAGCGCATCTCCTATGC
AGCGGATCAGATGGCGTTGGTGGCGGTTGATCCGCGCACCCACTTTATCAAGGCAATTGTTGGAGGCGTCGATAGTCAAA
AAAGTGAATTTAATCGGGCGACTCAATCCATTCGCCAACCGGGATCTGCATTTAAGCCCTTTGTCTTCTATGCTGCCTAT
GCGTCCGGTAAATATACGCCTAGCAGCGTCATCAATGACACCAAGGTCACCTTTAATGACGGGACCGCACGAGGCTATAC
GCCCTTGAACTATGACAAAACCTATGGGGGGTCCATGTCTTTGCGACAAGCCTTGGCCACCTCTCGTAATATTCCGGCTG
TTGTCCTAGGCCAGAAAGTCGGGGTTGAAAAAGTGATTGATATTTGTCGGGTTCTTGGCATTAAAAGCCCCATTCCTCCC
GTGATTTCACTACCGTTAGGTTCTGTAGACTTAACGCCTCTCGAAATGGCTTCAGCTTATGCAACGTTTGCTAATAACGG
CTGGCAATCTGAAACCACAGCCATTGCCCAAGTCACCGATAGTAGCGGGCGCTTAATCCTAGACAATACTCCCCAGCCCA
AGTTAGTGCTAGATCCCTGGGCAGCTGCAGCTTTAACCCAATCCCTGCAGGCAGTCATCAATGGCGGGACAGGCACCTCG
GCTCAAATTGGCCGACCGGCTGCAGGTAAAACGGGAACCACATCTTCTGAGCGGGATGTTTGGTTTGTGGGCTATGTGCC
GCAATTAGCTACGGCAGTGTGGGCCGGAAATGATAACTATTCCCCCATGGGAAGAGGGGCTTCAGGGGGGAGCTATGCGG
CACCAGTCTGGCGAGATTTTATGAGCAAAGCTCTGGCAGATCAACGGGTGGAATACTTTAAGTCACCTAGTAAGTTTCCC
AAGCCTGGCGGTTAG

Upstream 100 bases:

>100_bases
GCTATCTCTATCGACCTCCTGGTTGTCTGGGCCAAAATTGTTAGTATGATCCCTGAGTGATCATTGTTTCAAACTTTCCA
AAAGAGGATGTGAATCTCCT

Downstream 100 bases:

>100_bases
TTTAGGCCAGATGTCATGGGTCTAACAGGCTAGAAGGCTAGATAGCCTGCTTTATAGTTCTATCGACGGAACGCGAAACC
TGCGATGACATCTTATCAAC

Product: penicillin-binding protein 1A (PBP-1a)

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MSVSQTASPKNPNRLSKGTSFIGGIGKAAGSTILITTMLASAAAAGSLMGVAISFRNLPDVRSLKGYIPNETTHIYDING
KILASLHDEENREVVPLDNISPHLKLAVLAIEDSNFYKHNGINPYGILRATVTNIQAGETVQGASTLTMQLVKNLFLTPR
RELGRKVAEAVLAMRLEKLFKKDEILEMYLNQVYWGHNTYGVETAARSYFNKSSSDLNLAESAMMAGIIQAPESFSPFDF
DSVTDETLEIAKRRQAVVLNRMKKLTWITEEEEQAALDTPLKFGKVTSFQLSRSPYITNTVIKELTRRFGRDALSKGGMR
IQTTIDIKMQEIAEDSVKWGQQRISYAADQMALVAVDPRTHFIKAIVGGVDSQKSEFNRATQSIRQPGSAFKPFVFYAAY
ASGKYTPSSVINDTKVTFNDGTARGYTPLNYDKTYGGSMSLRQALATSRNIPAVVLGQKVGVEKVIDICRVLGIKSPIPP
VISLPLGSVDLTPLEMASAYATFANNGWQSETTAIAQVTDSSGRLILDNTPQPKLVLDPWAAAALTQSLQAVINGGTGTS
AQIGRPAAGKTGTTSSERDVWFVGYVPQLATAVWAGNDNYSPMGRGASGGSYAAPVWRDFMSKALADQRVEYFKSPSKFP
KPGG

Sequences:

>Translated_644_residues
MSVSQTASPKNPNRLSKGTSFIGGIGKAAGSTILITTMLASAAAAGSLMGVAISFRNLPDVRSLKGYIPNETTHIYDING
KILASLHDEENREVVPLDNISPHLKLAVLAIEDSNFYKHNGINPYGILRATVTNIQAGETVQGASTLTMQLVKNLFLTPR
RELGRKVAEAVLAMRLEKLFKKDEILEMYLNQVYWGHNTYGVETAARSYFNKSSSDLNLAESAMMAGIIQAPESFSPFDF
DSVTDETLEIAKRRQAVVLNRMKKLTWITEEEEQAALDTPLKFGKVTSFQLSRSPYITNTVIKELTRRFGRDALSKGGMR
IQTTIDIKMQEIAEDSVKWGQQRISYAADQMALVAVDPRTHFIKAIVGGVDSQKSEFNRATQSIRQPGSAFKPFVFYAAY
ASGKYTPSSVINDTKVTFNDGTARGYTPLNYDKTYGGSMSLRQALATSRNIPAVVLGQKVGVEKVIDICRVLGIKSPIPP
VISLPLGSVDLTPLEMASAYATFANNGWQSETTAIAQVTDSSGRLILDNTPQPKLVLDPWAAAALTQSLQAVINGGTGTS
AQIGRPAAGKTGTTSSERDVWFVGYVPQLATAVWAGNDNYSPMGRGASGGSYAAPVWRDFMSKALADQRVEYFKSPSKFP
KPGG
>Mature_643_residues
SVSQTASPKNPNRLSKGTSFIGGIGKAAGSTILITTMLASAAAAGSLMGVAISFRNLPDVRSLKGYIPNETTHIYDINGK
ILASLHDEENREVVPLDNISPHLKLAVLAIEDSNFYKHNGINPYGILRATVTNIQAGETVQGASTLTMQLVKNLFLTPRR
ELGRKVAEAVLAMRLEKLFKKDEILEMYLNQVYWGHNTYGVETAARSYFNKSSSDLNLAESAMMAGIIQAPESFSPFDFD
SVTDETLEIAKRRQAVVLNRMKKLTWITEEEEQAALDTPLKFGKVTSFQLSRSPYITNTVIKELTRRFGRDALSKGGMRI
QTTIDIKMQEIAEDSVKWGQQRISYAADQMALVAVDPRTHFIKAIVGGVDSQKSEFNRATQSIRQPGSAFKPFVFYAAYA
SGKYTPSSVINDTKVTFNDGTARGYTPLNYDKTYGGSMSLRQALATSRNIPAVVLGQKVGVEKVIDICRVLGIKSPIPPV
ISLPLGSVDLTPLEMASAYATFANNGWQSETTAIAQVTDSSGRLILDNTPQPKLVLDPWAAAALTQSLQAVINGGTGTSA
QIGRPAAGKTGTTSSERDVWFVGYVPQLATAVWAGNDNYSPMGRGASGGSYAAPVWRDFMSKALADQRVEYFKSPSKFPK
PGG

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=541, Percent_Identity=33.271719038817, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=37.4592833876222, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1788867, Length=542, Percent_Identity=29.1512915129151, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789601, Length=174, Percent_Identity=36.7816091954023, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 69887; Mature: 69756

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSQTASPKNPNRLSKGTSFIGGIGKAAGSTILITTMLASAAAAGSLMGVAISFRNLPD
CCCCCCCCCCCCCHHHHCCHHHHCHHHHCCCEEHHHHHHHHHHHHHHHHEEEEECCCCCC
VRSLKGYIPNETTHIYDINGKILASLHDEENREVVPLDNISPHLKLAVLAIEDSNFYKHN
HHHHCCCCCCCCCEEEECCCEEEEEECCCCCCEEEECCCCCCCEEEEEEEEECCCCEECC
GINPYGILRATVTNIQAGETVQGASTLTMQLVKNLFLTPRRELGRKVAEAVLAMRLEKLF
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KKDEILEMYLNQVYWGHNTYGVETAARSYFNKSSSDLNLAESAMMAGIIQAPESFSPFDF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
DSVTDETLEIAKRRQAVVLNRMKKLTWITEEEEQAALDTPLKFGKVTSFQLSRSPYITNT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHH
VIKELTRRFGRDALSKGGMRIQTTIDIKMQEIAEDSVKWGQQRISYAADQMALVAVDPRT
HHHHHHHHHCHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHH
HFIKAIVGGVDSQKSEFNRATQSIRQPGSAFKPFVFYAAYASGKYTPSSVINDTKVTFND
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHCCCCEEEECC
GTARGYTPLNYDKTYGGSMSLRQALATSRNIPAVVLGQKVGVEKVIDICRVLGIKSPIPP
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCC
VISLPLGSVDLTPLEMASAYATFANNGWQSETTAIAQVTDSSGRLILDNTPQPKLVLDPW
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCEEECCH
AAAALTQSLQAVINGGTGTSAQIGRPAAGKTGTTSSERDVWFVGYVPQLATAVWAGNDNY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHEEECCCCCC
SPMGRGASGGSYAAPVWRDFMSKALADQRVEYFKSPSKFPKPGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SVSQTASPKNPNRLSKGTSFIGGIGKAAGSTILITTMLASAAAAGSLMGVAISFRNLPD
CCCCCCCCCCCCHHHHCCHHHHCHHHHCCCEEHHHHHHHHHHHHHHHHEEEEECCCCCC
VRSLKGYIPNETTHIYDINGKILASLHDEENREVVPLDNISPHLKLAVLAIEDSNFYKHN
HHHHCCCCCCCCCEEEECCCEEEEEECCCCCCEEEECCCCCCCEEEEEEEEECCCCEECC
GINPYGILRATVTNIQAGETVQGASTLTMQLVKNLFLTPRRELGRKVAEAVLAMRLEKLF
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KKDEILEMYLNQVYWGHNTYGVETAARSYFNKSSSDLNLAESAMMAGIIQAPESFSPFDF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
DSVTDETLEIAKRRQAVVLNRMKKLTWITEEEEQAALDTPLKFGKVTSFQLSRSPYITNT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHH
VIKELTRRFGRDALSKGGMRIQTTIDIKMQEIAEDSVKWGQQRISYAADQMALVAVDPRT
HHHHHHHHHCHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHH
HFIKAIVGGVDSQKSEFNRATQSIRQPGSAFKPFVFYAAYASGKYTPSSVINDTKVTFND
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHCCCCEEEECC
GTARGYTPLNYDKTYGGSMSLRQALATSRNIPAVVLGQKVGVEKVIDICRVLGIKSPIPP
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCC
VISLPLGSVDLTPLEMASAYATFANNGWQSETTAIAQVTDSSGRLILDNTPQPKLVLDPW
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCEEECCH
AAAALTQSLQAVINGGTGTSAQIGRPAAGKTGTTSSERDVWFVGYVPQLATAVWAGNDNY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHEEECCCCCC
SPMGRGASGGSYAAPVWRDFMSKALADQRVEYFKSPSKFPKPGG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]