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The map label for this gene is cbiB [H]

Identifier: 158336462

GI number: 158336462

Start: 3358626

End: 3359645

Strand: Direct

Name: cbiB [H]

Synonym: AM1_3326

Alternate gene names: 158336462

Gene position: 3358626-3359645 (Clockwise)

Preceding gene: 158336461

Following gene: 158336465

Centisome position: 51.64

GC content: 51.96

Gene sequence:

>1020_bases
GTGATCAGCTCTGATACTGGAGTAGCTTTTGACCAGCCGTTAGCCATCATGGTGCTGATCAGTGCAGCAGTGCTTGATTT
TGCCATTGGAGATCCCTGGTGGTGTCCTCATCCTGTGCAAATGATGGGATGGATCGTCCAGCGCTATACCAAGGGAGCAT
TGCGGTGGTGGAATGAACCGTGGCAACTGCGGATAGCGGGTATACATCTGACGATTGCCCTGGTCTGTAGTGTGAGCTTG
GGCAGCTGGCTGCTGTTTTATGGGTTAGATCGATGGGCTTGGCCGGTTAGTTTGGGATTAGAAGTTGTTTTGCTCGCCAG
TTGTTTTGCGGGACGTAGTTTACATGATGCAGCAGTTGATGTTCTCAAACCGTTAGAGAGAGCGGATTTGACCGTGGCGA
GATCGCGGCTGCGTCGCTATGTCGGGAGAGACACAGATACCTTGGCAGAGCAAGATATCTTAAGGGCTTTACTGGAGACT
GTTGCTGAGAATGCCACGGATGGTGTGATGGCTCCTTTGTTTTATGCTCTAGTCGGTCTTGCCATTCCTGGAGTGGGGCC
TGTGCCTGTGGCGTTGTTCTACAAGGCAGTTAGTACATTAGATTCTATGATTGGGTATCGCACGGCTCCCTACACTGATC
TGGGTTGGTTTAGTGCGCGCCTCGATGATGTTTTGACCTGGTTTCCTTGCCGATTGATGGTGCTGACGGTGGGGTTGTTA
TCGGGAAAACTCAAGTCTGTCTGGGTGATTGGTTGGCGAGATGCTCGTAAGGACCCTAGCCCTAACTCAGGTTGGAGTGA
ATGTGCCTATGCTGCCATTTTGGGTGTGCAGTTGGGGGGACAAAATTGGTACCGGGGTGTGGCTAAGTTGAAGCCCCTGC
TGGGCAATCCCCTGCAACGGATTACTGAGAAGCATATCGAGGTGGCGATTCGCCTGACCCGCAATTGTTTTTTGCTGTGG
TTGGGATTGGCAGTGGCCTTCACTTGGTTGACAATGCCAACCCCTATGTCCTGGGGCTAA

Upstream 100 bases:

>100_bases
CTTATCCCTTAAGACAGTCAGTCCTCAAGCCAAGTTGAAGATGATTTTTGCTCCCATCATTCATCGATTTATCTCAATAT
TTGTGTGTGCTTTGTAGCCG

Downstream 100 bases:

>100_bases
TGGTATCAGTTGTCTTTGAGTAGTAAATAATAGAGCTGCCCGGATCCCGTCATGACTCCTGCCCGTTCTTTTGCTTTTGC
CCCAGTGCCCATAAAAATAT

Product: cobalamin biosynthesis protein CbiB

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MISSDTGVAFDQPLAIMVLISAAVLDFAIGDPWWCPHPVQMMGWIVQRYTKGALRWWNEPWQLRIAGIHLTIALVCSVSL
GSWLLFYGLDRWAWPVSLGLEVVLLASCFAGRSLHDAAVDVLKPLERADLTVARSRLRRYVGRDTDTLAEQDILRALLET
VAENATDGVMAPLFYALVGLAIPGVGPVPVALFYKAVSTLDSMIGYRTAPYTDLGWFSARLDDVLTWFPCRLMVLTVGLL
SGKLKSVWVIGWRDARKDPSPNSGWSECAYAAILGVQLGGQNWYRGVAKLKPLLGNPLQRITEKHIEVAIRLTRNCFLLW
LGLAVAFTWLTMPTPMSWG

Sequences:

>Translated_339_residues
MISSDTGVAFDQPLAIMVLISAAVLDFAIGDPWWCPHPVQMMGWIVQRYTKGALRWWNEPWQLRIAGIHLTIALVCSVSL
GSWLLFYGLDRWAWPVSLGLEVVLLASCFAGRSLHDAAVDVLKPLERADLTVARSRLRRYVGRDTDTLAEQDILRALLET
VAENATDGVMAPLFYALVGLAIPGVGPVPVALFYKAVSTLDSMIGYRTAPYTDLGWFSARLDDVLTWFPCRLMVLTVGLL
SGKLKSVWVIGWRDARKDPSPNSGWSECAYAAILGVQLGGQNWYRGVAKLKPLLGNPLQRITEKHIEVAIRLTRNCFLLW
LGLAVAFTWLTMPTPMSWG
>Mature_339_residues
MISSDTGVAFDQPLAIMVLISAAVLDFAIGDPWWCPHPVQMMGWIVQRYTKGALRWWNEPWQLRIAGIHLTIALVCSVSL
GSWLLFYGLDRWAWPVSLGLEVVLLASCFAGRSLHDAAVDVLKPLERADLTVARSRLRRYVGRDTDTLAEQDILRALLET
VAENATDGVMAPLFYALVGLAIPGVGPVPVALFYKAVSTLDSMIGYRTAPYTDLGWFSARLDDVLTWFPCRLMVLTVGLL
SGKLKSVWVIGWRDARKDPSPNSGWSECAYAAILGVQLGGQNWYRGVAKLKPLLGNPLQRITEKHIEVAIRLTRNCFLLW
LGLAVAFTWLTMPTPMSWG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 37642; Mature: 37642

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSDTGVAFDQPLAIMVLISAAVLDFAIGDPWWCPHPVQMMGWIVQRYTKGALRWWNEP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
WQLRIAGIHLTIALVCSVSLGSWLLFYGLDRWAWPVSLGLEVVLLASCFAGRSLHDAAVD
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VLKPLERADLTVARSRLRRYVGRDTDTLAEQDILRALLETVAENATDGVMAPLFYALVGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AIPGVGPVPVALFYKAVSTLDSMIGYRTAPYTDLGWFSARLDDVLTWFPCRLMVLTVGLL
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKLKSVWVIGWRDARKDPSPNSGWSECAYAAILGVQLGGQNWYRGVAKLKPLLGNPLQR
CCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHH
ITEKHIEVAIRLTRNCFLLWLGLAVAFTWLTMPTPMSWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MISSDTGVAFDQPLAIMVLISAAVLDFAIGDPWWCPHPVQMMGWIVQRYTKGALRWWNEP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
WQLRIAGIHLTIALVCSVSLGSWLLFYGLDRWAWPVSLGLEVVLLASCFAGRSLHDAAVD
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VLKPLERADLTVARSRLRRYVGRDTDTLAEQDILRALLETVAENATDGVMAPLFYALVGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AIPGVGPVPVALFYKAVSTLDSMIGYRTAPYTDLGWFSARLDDVLTWFPCRLMVLTVGLL
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKLKSVWVIGWRDARKDPSPNSGWSECAYAAILGVQLGGQNWYRGVAKLKPLLGNPLQR
CCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHH
ITEKHIEVAIRLTRNCFLLWLGLAVAFTWLTMPTPMSWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]