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The map label for this gene is pykF [H]

Identifier: 158336451

GI number: 158336451

Start: 3346337

End: 3348112

Strand: Reverse

Name: pykF [H]

Synonym: AM1_3315

Alternate gene names: 158336451

Gene position: 3348112-3346337 (Counterclockwise)

Preceding gene: 158336453

Following gene: 158336450

Centisome position: 51.48

GC content: 52.7

Gene sequence:

>1776_bases
ATGTCCTTGCCTGAGACCGACCGTCGTACCAAAATTGTGGCCACCGTAGGGCCAGCCATCTCCAAGCCAGATATGCTGCG
GGCGGTCATTGAAGCAGGGGCCACCACCCTCCGCCTCAACTTCTCCCACGGCACCCATGACGATCATCTGCGATCCATTC
GTCTGATTCGGCAACTCTCTTATGAGATGAACCAGCCCGTTGCCATTCTGCAAGACCTGCAAGGGCCTAAAATCCGCCTT
GGTAAATTCGCGGACGGCCCCATCACCCTCAAAAACGGTGATCCCTTTGCCTTGACGAGTCAGGCTATTCCCGGCACGCA
AACCAGCAGCATGGTCACCTATTCCAAACTGGCTGACGAAGTGTCTGCAGGAGCCACCATTCTTCTCGATGATGGCCGAG
TCGAGATGATCGTTGAATCTGTAGATCAATCTTCTAAGACCTTGAATTGCCGCGTCGTTGTGGGAGGCAAGCTCTCGGAC
TCTAAAGGTGTGAACTTTCCAGGAGTCTATTTATCCGTCAAAGCGCTCACCGATAAAGATCGCGAAGATTTACAATTCGG
TCTGACCCATGGTGTCGATTGGGTTGCCCTCAGCTTCGTCCGCAATCCAGCAGACGTGATCGAGATTAAAGAAATCATTG
CTAACGCAGGCAAATCCATCCCCGTCATTGCCAAGATCGAAAAACATGAAGCCATTACCCAAATGGAAGAGATTCTCAGC
CTATGTGATGGGGTAATGATCGCTCGGGGTGACTTAGGGGTTGAGCTGCCTGCAGAGGATGTCCCGATTCTGCAAAAGCG
GTTGATTGCCACGGCAAACCGTCTCGGCATTCCTGTGATTACGGCCACGCAAATGCTGGATAGCATGGTCAATTCCCCCA
GACCCACGCGGGCAGAGGTCTCTGACGTGGCCAACGCCATTTTGGACGGCACTGATGCGGTGATGCTGTCCAATGAAGCC
GCCGTCGGCAACTATCCTGTGGAAGCGGTGGCGACCATGGCCAAAATCGCAGAGCGGATTGATTATGAAAAAAATATTCA
AAATCGTACTGCCCTAGACACCTCAGAAACTGCCATTCCCAGTGCCATTAGCCAAGCGGTTGGGCATATCGCCACTCAGC
TCAAAGCCAAAGCGATTCTCACCTTTACCAAAACTGGATCGACCGGACGCAACGTTTCCAAATATCGGCCCAATATTCCC
ATTCTGGCTGTGACGCCCCATGTAGAGGTCGCTCGCCAACTGCAAATGGTTTGGGGTGTACAGCCGTTGCTGGTTCTCAA
TTTGCCCTCCATTGATGAAACCCTACAAGCCTCACTCAATGTGGCCCAGGAGAAAGGCTTAGTGGAAGAAGGCGACTTAG
TAGTGGTGACCGCAGGCACTCTCCAGGGGGTCGCGGGGTCCACAGACTTTATCAAAGTCGAGATTGTCACCGCTGTTATG
GGTCAGGGCACTGGCATTGGCAATGGCTCTGTGAGCGGCTTAGCTCGCGTCATTACAGCATCCAACGGTGTTAAAGGCTT
TAATCGGGGCGATATTTTAGTAGCGAAGGAAACTGACGCTAGCCATGTCGATGCGATTCGCAAAGCCGCTGGCATCATTA
CTGAAGTCGGCGGTATGGACAGTCACGCTGCCGTATTAGGGCTGCGGTTGCGAATTCCAGTCATCGTCGGGGTTGAAAAT
GCAACTCGGATGATTCGGGATGGCACCCACCTTACGTTAGATATGAAAAAAGGGTTGGTCTACTCGGGCACCATGACGTC
GCAAGCCCAAAGCTAA

Upstream 100 bases:

>100_bases
CAAAAATTCGCTTGGGGTAACGTCTCAGCCTAAACTGTTAAGTTGATGGGCAACCCTGTTGATTGTTCATCTTACTGCCT
AAACTTTTTTGAAATCATAC

Downstream 100 bases:

>100_bases
CCCCACCAATACACCTAATTGGAGTTCACGTACTAGACTGGATCTTGGAGTCGCATCCTACCCACTCGATGCTTTGAGTT
CATGAACACCATTGTTAGCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSLPETDRRTKIVATVGPAISKPDMLRAVIEAGATTLRLNFSHGTHDDHLRSIRLIRQLSYEMNQPVAILQDLQGPKIRL
GKFADGPITLKNGDPFALTSQAIPGTQTSSMVTYSKLADEVSAGATILLDDGRVEMIVESVDQSSKTLNCRVVVGGKLSD
SKGVNFPGVYLSVKALTDKDREDLQFGLTHGVDWVALSFVRNPADVIEIKEIIANAGKSIPVIAKIEKHEAITQMEEILS
LCDGVMIARGDLGVELPAEDVPILQKRLIATANRLGIPVITATQMLDSMVNSPRPTRAEVSDVANAILDGTDAVMLSNEA
AVGNYPVEAVATMAKIAERIDYEKNIQNRTALDTSETAIPSAISQAVGHIATQLKAKAILTFTKTGSTGRNVSKYRPNIP
ILAVTPHVEVARQLQMVWGVQPLLVLNLPSIDETLQASLNVAQEKGLVEEGDLVVVTAGTLQGVAGSTDFIKVEIVTAVM
GQGTGIGNGSVSGLARVITASNGVKGFNRGDILVAKETDASHVDAIRKAAGIITEVGGMDSHAAVLGLRLRIPVIVGVEN
ATRMIRDGTHLTLDMKKGLVYSGTMTSQAQS

Sequences:

>Translated_591_residues
MSLPETDRRTKIVATVGPAISKPDMLRAVIEAGATTLRLNFSHGTHDDHLRSIRLIRQLSYEMNQPVAILQDLQGPKIRL
GKFADGPITLKNGDPFALTSQAIPGTQTSSMVTYSKLADEVSAGATILLDDGRVEMIVESVDQSSKTLNCRVVVGGKLSD
SKGVNFPGVYLSVKALTDKDREDLQFGLTHGVDWVALSFVRNPADVIEIKEIIANAGKSIPVIAKIEKHEAITQMEEILS
LCDGVMIARGDLGVELPAEDVPILQKRLIATANRLGIPVITATQMLDSMVNSPRPTRAEVSDVANAILDGTDAVMLSNEA
AVGNYPVEAVATMAKIAERIDYEKNIQNRTALDTSETAIPSAISQAVGHIATQLKAKAILTFTKTGSTGRNVSKYRPNIP
ILAVTPHVEVARQLQMVWGVQPLLVLNLPSIDETLQASLNVAQEKGLVEEGDLVVVTAGTLQGVAGSTDFIKVEIVTAVM
GQGTGIGNGSVSGLARVITASNGVKGFNRGDILVAKETDASHVDAIRKAAGIITEVGGMDSHAAVLGLRLRIPVIVGVEN
ATRMIRDGTHLTLDMKKGLVYSGTMTSQAQS
>Mature_590_residues
SLPETDRRTKIVATVGPAISKPDMLRAVIEAGATTLRLNFSHGTHDDHLRSIRLIRQLSYEMNQPVAILQDLQGPKIRLG
KFADGPITLKNGDPFALTSQAIPGTQTSSMVTYSKLADEVSAGATILLDDGRVEMIVESVDQSSKTLNCRVVVGGKLSDS
KGVNFPGVYLSVKALTDKDREDLQFGLTHGVDWVALSFVRNPADVIEIKEIIANAGKSIPVIAKIEKHEAITQMEEILSL
CDGVMIARGDLGVELPAEDVPILQKRLIATANRLGIPVITATQMLDSMVNSPRPTRAEVSDVANAILDGTDAVMLSNEAA
VGNYPVEAVATMAKIAERIDYEKNIQNRTALDTSETAIPSAISQAVGHIATQLKAKAILTFTKTGSTGRNVSKYRPNIPI
LAVTPHVEVARQLQMVWGVQPLLVLNLPSIDETLQASLNVAQEKGLVEEGDLVVVTAGTLQGVAGSTDFIKVEIVTAVMG
QGTGIGNGSVSGLARVITASNGVKGFNRGDILVAKETDASHVDAIRKAAGIITEVGGMDSHAAVLGLRLRIPVIVGVENA
TRMIRDGTHLTLDMKKGLVYSGTMTSQAQS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=499, Percent_Identity=38.877755511022, Blast_Score=324, Evalue=2e-88,
Organism=Homo sapiens, GI32967597, Length=499, Percent_Identity=38.877755511022, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=37.5757575757576, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI33286422, Length=494, Percent_Identity=36.4372469635627, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI33286420, Length=494, Percent_Identity=36.4372469635627, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI310128732, Length=295, Percent_Identity=37.6271186440678, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128730, Length=295, Percent_Identity=37.6271186440678, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128736, Length=243, Percent_Identity=33.7448559670782, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI310128734, Length=243, Percent_Identity=33.7448559670782, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=32.8703703703704, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1787965, Length=457, Percent_Identity=43.3260393873085, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=40.5857740585774, Blast_Score=309, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17544584, Length=492, Percent_Identity=39.4308943089431, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=39.7959183673469, Blast_Score=311, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=39.7959183673469, Blast_Score=311, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=39.7959183673469, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17506831, Length=490, Percent_Identity=39.7959183673469, Blast_Score=309, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=41.1522633744856, Blast_Score=325, Evalue=9e-90,
Organism=Saccharomyces cerevisiae, GI6324923, Length=497, Percent_Identity=36.6197183098592, Blast_Score=288, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=40.6952965235174, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=40.6952965235174, Blast_Score=342, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=39.5121951219512, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=32.0408163265306, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24646914, Length=313, Percent_Identity=38.6581469648562, Blast_Score=195, Evalue=7e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62815; Mature: 62684

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPETDRRTKIVATVGPAISKPDMLRAVIEAGATTLRLNFSHGTHDDHLRSIRLIRQLS
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
YEMNQPVAILQDLQGPKIRLGKFADGPITLKNGDPFALTSQAIPGTQTSSMVTYSKLADE
HHCCCCHHHHHCCCCCCEEECEECCCCEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHH
VSAGATILLDDGRVEMIVESVDQSSKTLNCRVVVGGKLSDSKGVNFPGVYLSVKALTDKD
HCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCC
REDLQFGLTHGVDWVALSFVRNPADVIEIKEIIANAGKSIPVIAKIEKHEAITQMEEILS
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
LCDGVMIARGDLGVELPAEDVPILQKRLIATANRLGIPVITATQMLDSMVNSPRPTRAEV
HHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNEAAVGNYPVEAVATMAKIAERIDYEKNIQNRTALDTSETAIP
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHH
SAISQAVGHIATQLKAKAILTFTKTGSTGRNVSKYRPNIPILAVTPHVEVARQLQMVWGV
HHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHCC
QPLLVLNLPSIDETLQASLNVAQEKGLVEEGDLVVVTAGTLQGVAGSTDFIKVEIVTAVM
CCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEHH
GQGTGIGNGSVSGLARVITASNGVKGFNRGDILVAKETDASHVDAIRKAAGIITEVGGMD
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
SHAAVLGLRLRIPVIVGVENATRMIRDGTHLTLDMKKGLVYSGTMTSQAQS
CCHHEEEEEEEEEEEEECCHHHHHHHCCCEEEEEECCCEEEECCCCCCCCC
>Mature Secondary Structure 
SLPETDRRTKIVATVGPAISKPDMLRAVIEAGATTLRLNFSHGTHDDHLRSIRLIRQLS
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
YEMNQPVAILQDLQGPKIRLGKFADGPITLKNGDPFALTSQAIPGTQTSSMVTYSKLADE
HHCCCCHHHHHCCCCCCEEECEECCCCEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHH
VSAGATILLDDGRVEMIVESVDQSSKTLNCRVVVGGKLSDSKGVNFPGVYLSVKALTDKD
HCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCC
REDLQFGLTHGVDWVALSFVRNPADVIEIKEIIANAGKSIPVIAKIEKHEAITQMEEILS
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
LCDGVMIARGDLGVELPAEDVPILQKRLIATANRLGIPVITATQMLDSMVNSPRPTRAEV
HHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNEAAVGNYPVEAVATMAKIAERIDYEKNIQNRTALDTSETAIP
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHH
SAISQAVGHIATQLKAKAILTFTKTGSTGRNVSKYRPNIPILAVTPHVEVARQLQMVWGV
HHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHCC
QPLLVLNLPSIDETLQASLNVAQEKGLVEEGDLVVVTAGTLQGVAGSTDFIKVEIVTAVM
CCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEHH
GQGTGIGNGSVSGLARVITASNGVKGFNRGDILVAKETDASHVDAIRKAAGIITEVGGMD
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
SHAAVLGLRLRIPVIVGVENATRMIRDGTHLTLDMKKGLVYSGTMTSQAQS
CCHHEEEEEEEEEEEEECCHHHHHHHCCCEEEEEECCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]