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The map label for this gene is ydaP [H]

Identifier: 158336445

GI number: 158336445

Start: 3340549

End: 3342501

Strand: Reverse

Name: ydaP [H]

Synonym: AM1_3309

Alternate gene names: 158336445

Gene position: 3342501-3340549 (Counterclockwise)

Preceding gene: 158336447

Following gene: 158336444

Centisome position: 51.39

GC content: 53.15

Gene sequence:

>1953_bases
ATGCCTGAGATAACCCCTCAGTGGTTTCGGGTCTTAGACCTAGACGAACTACCCGAAGGTCGGGTCAAAACCGTCGTTGC
AGGTCATAAAAGTCTGGCCTTAACCCATCACCAAGGCCAGTATGGTGCCCTTGATAATCGCTGTCCTCACCAAGGCGGCC
CCCTCGGCGAAGGCTCTATTGAAAATGGCCTGTTGCGCTGTCCTTGGCATGGGTGGGATTATTGCCCACTGACGGGGCAA
CCGCCCGGTGGCTTGGATGTCGATGATGCCCTCGAAACCTTTCCGGTTGAGGTGCGAGATGACGGTGTCTATGTGGGTTT
ACCCGTGGAGAAACCGCACATCCGCACCGTTAGCGATGTGATGGCTGAAACCATGGTGAATTGGGGCGTTACCCATGTAT
TTGGTATGGTAGGCCATTCGAATTTAGGACTGGCTGATACCCTACGCCGTCAGGAAGAACAGGGCAAGCTCACCTATATT
GGCATTCGCCATGAAGGAGCCGCAGCCTTTGCTGCCTCCGCCTATGCCAAACTCACGGGTACCCCCGCTGCTTGTTTGAC
CATTGCTGGCCCCGGTGCAACCAACCTCCTGACCGGAATGTGGGACGCCAAAGTCGATTGTGCCCCCATCTTGGCTTTAA
CCGGACAGGTCAATTCTCAAGTCTTGGGGACTGGTAACTTTCAAGAATGTGATTTATCTGCCGCCTTTAGTGGGGTGGCA
GCTTGGAGTCAAACGGTTCTTAGTCACAGCAAGCATGCCGAATTGATGACTCTGGCGATCAAGCATGCTCTCTTAGAACG
CAATGTCTCTCACCTGATTTTTCCCGATGAGATTCAAGTCCAGCCTGCTGTTGATGTCCCTGCGGCAACGCCCACTGGAC
GCATCACCCCGCTGCAAATCGCGCCACCCCCGGAGTCTTTACAGCAGGCCATTGGCAAACTGAAACAGTCACGCCGCCCC
GTCATCATTGTGGGTCATGGCGCTCGGTTTCATATGCCTGCGGTGATAGAACTAGCTGAACTTTTGGGCGCGCCCGTCCT
AACAACCTTTAAAGGTAAAGGGCAGATTGCCGATGACCATCCTCTGGGATGTGGAGTATTGGGGCGCAGTGGTACTCCCA
TTGCCAGCTGGTTTATGAATGAGGCGGATGTGCTGTTGGTATTGGGGGCTTCTTTTTCTAATCACACGGGCATCTATCCA
GGCAAACCGATTATTCAGGTAGATTTTGATCCGCTGGCCCTAGGTAAATTTCATCCCATCGAAGTTCCCGTTTGGGGGGA
AATTAGTATTGTGGTGGAGCAGCTCAAGACCGCCCTCTCGATGCAGGTGGATACGGAGGATGTGCAATCTGAAATCGCCG
AACGTTGGGCCATCTGGCGGGCAGAAAAAGCCCGGCGAGAATTGGATGACCGAGGACAAGGGCTAAACTCTGCCGCTATT
TTTGCCGCCATGACGCGATGGGTGCCTGCTCATGCTGTAATTGCGGTGGATGTAGGCAACAACACCTATTCCTTTGGTCG
ATATTTTGAGTGTCAGGCTCAGTCCGTTTTAATGTCGGGATATCTCGGGTCCATCGGGTTTGCCTTCCCGGCTGCCATGG
GCGCTTGGGCTGCCGCAGGCCATAAGCGTCCCATTGTGTCTGTATCAGGTGATGGCGGGTTTGGACAATATTTGGCGGAA
TTTACCACCGCCGTGAAGTACCAGATGAATATCACCCATATCCTGCTCCATAACGATCAGTTGGGCAAAATTTCTAAGGA
GCAGAGAGCGGGGGAATGGGATGTCTGGCAAACCTCCTTACACAATCCTGATTTTTCCCAATATGCTCAGAATTGCGGTG
GATTTGGAATCCGAGTCACCCAACCGGACGAACTAGATGATGCTATGGCCCAGGCCCTGGCTTATCAGGGGCCTGCACTC
GTAGAAATCATGTCTGACCCAGAATTGATTTAG

Upstream 100 bases:

>100_bases
ACCCAACCTGCATACTCAGACTGGAGCCTATAAACCAGCATCAACCTGACAAAAAGTGCTGAGCCAGCCATCGCCGCTCA
TCCCATAAGGAGTACAGCCT

Downstream 100 bases:

>100_bases
GCTGTCTGGTGCGGCCTACACCTTGAACCTATAAGACGTTTATACATCTACCCAGAGGAATTATGTCTGAACAGCTTACG
AACATCACTCAGCACCAATG

Product: thiamine pyrophosphate enzyme

Products: 2-acetolactate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MPEITPQWFRVLDLDELPEGRVKTVVAGHKSLALTHHQGQYGALDNRCPHQGGPLGEGSIENGLLRCPWHGWDYCPLTGQ
PPGGLDVDDALETFPVEVRDDGVYVGLPVEKPHIRTVSDVMAETMVNWGVTHVFGMVGHSNLGLADTLRRQEEQGKLTYI
GIRHEGAAAFAASAYAKLTGTPAACLTIAGPGATNLLTGMWDAKVDCAPILALTGQVNSQVLGTGNFQECDLSAAFSGVA
AWSQTVLSHSKHAELMTLAIKHALLERNVSHLIFPDEIQVQPAVDVPAATPTGRITPLQIAPPPESLQQAIGKLKQSRRP
VIIVGHGARFHMPAVIELAELLGAPVLTTFKGKGQIADDHPLGCGVLGRSGTPIASWFMNEADVLLVLGASFSNHTGIYP
GKPIIQVDFDPLALGKFHPIEVPVWGEISIVVEQLKTALSMQVDTEDVQSEIAERWAIWRAEKARRELDDRGQGLNSAAI
FAAMTRWVPAHAVIAVDVGNNTYSFGRYFECQAQSVLMSGYLGSIGFAFPAAMGAWAAAGHKRPIVSVSGDGGFGQYLAE
FTTAVKYQMNITHILLHNDQLGKISKEQRAGEWDVWQTSLHNPDFSQYAQNCGGFGIRVTQPDELDDAMAQALAYQGPAL
VEIMSDPELI

Sequences:

>Translated_650_residues
MPEITPQWFRVLDLDELPEGRVKTVVAGHKSLALTHHQGQYGALDNRCPHQGGPLGEGSIENGLLRCPWHGWDYCPLTGQ
PPGGLDVDDALETFPVEVRDDGVYVGLPVEKPHIRTVSDVMAETMVNWGVTHVFGMVGHSNLGLADTLRRQEEQGKLTYI
GIRHEGAAAFAASAYAKLTGTPAACLTIAGPGATNLLTGMWDAKVDCAPILALTGQVNSQVLGTGNFQECDLSAAFSGVA
AWSQTVLSHSKHAELMTLAIKHALLERNVSHLIFPDEIQVQPAVDVPAATPTGRITPLQIAPPPESLQQAIGKLKQSRRP
VIIVGHGARFHMPAVIELAELLGAPVLTTFKGKGQIADDHPLGCGVLGRSGTPIASWFMNEADVLLVLGASFSNHTGIYP
GKPIIQVDFDPLALGKFHPIEVPVWGEISIVVEQLKTALSMQVDTEDVQSEIAERWAIWRAEKARRELDDRGQGLNSAAI
FAAMTRWVPAHAVIAVDVGNNTYSFGRYFECQAQSVLMSGYLGSIGFAFPAAMGAWAAAGHKRPIVSVSGDGGFGQYLAE
FTTAVKYQMNITHILLHNDQLGKISKEQRAGEWDVWQTSLHNPDFSQYAQNCGGFGIRVTQPDELDDAMAQALAYQGPAL
VEIMSDPELI
>Mature_649_residues
PEITPQWFRVLDLDELPEGRVKTVVAGHKSLALTHHQGQYGALDNRCPHQGGPLGEGSIENGLLRCPWHGWDYCPLTGQP
PGGLDVDDALETFPVEVRDDGVYVGLPVEKPHIRTVSDVMAETMVNWGVTHVFGMVGHSNLGLADTLRRQEEQGKLTYIG
IRHEGAAAFAASAYAKLTGTPAACLTIAGPGATNLLTGMWDAKVDCAPILALTGQVNSQVLGTGNFQECDLSAAFSGVAA
WSQTVLSHSKHAELMTLAIKHALLERNVSHLIFPDEIQVQPAVDVPAATPTGRITPLQIAPPPESLQQAIGKLKQSRRPV
IIVGHGARFHMPAVIELAELLGAPVLTTFKGKGQIADDHPLGCGVLGRSGTPIASWFMNEADVLLVLGASFSNHTGIYPG
KPIIQVDFDPLALGKFHPIEVPVWGEISIVVEQLKTALSMQVDTEDVQSEIAERWAIWRAEKARRELDDRGQGLNSAAIF
AAMTRWVPAHAVIAVDVGNNTYSFGRYFECQAQSVLMSGYLGSIGFAFPAAMGAWAAAGHKRPIVSVSGDGGFGQYLAEF
TTAVKYQMNITHILLHNDQLGKISKEQRAGEWDVWQTSLHNPDFSQYAQNCGGFGIRVTQPDELDDAMAQALAYQGPALV
EIMSDPELI

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=26.4448336252189, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=28.2051282051282, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081685, Length=547, Percent_Identity=27.6051188299817, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=29.0613718411552, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=26.1180679785331, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=25.045045045045, Blast_Score=126, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=586, Percent_Identity=27.3037542662116, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=586, Percent_Identity=27.3037542662116, Blast_Score=135, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=558, Percent_Identity=22.4014336917563, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=24.2372881355932, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320816, Length=542, Percent_Identity=25.0922509225092, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=29.6907216494845, Blast_Score=161, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6

Molecular weight: Translated: 70074; Mature: 69942

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEITPQWFRVLDLDELPEGRVKTVVAGHKSLALTHHQGQYGALDNRCPHQGGPLGEGSI
CCCCCHHHHEEECHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ENGLLRCPWHGWDYCPLTGQPPGGLDVDDALETFPVEVRDDGVYVGLPVEKPHIRTVSDV
CCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCEEEEECCCCCCCHHHHHH
MAETMVNWGVTHVFGMVGHSNLGLADTLRRQEEQGKLTYIGIRHEGAAAFAASAYAKLTG
HHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCC
TPAACLTIAGPGATNLLTGMWDAKVDCAPILALTGQVNSQVLGTGNFQECDLSAAFSGVA
CCCEEEEEECCCHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHH
AWSQTVLSHSKHAELMTLAIKHALLERNVSHLIFPDEIQVQPAVDVPAATPTGRITPLQI
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCCCCEEEEEE
APPPESLQQAIGKLKQSRRPVIIVGHGARFHMPAVIELAELLGAPVLTTFKGKGQIADDH
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCC
PLGCGVLGRSGTPIASWFMNEADVLLVLGASFSNHTGIYPGKPIIQVDFDPLALGKFHPI
CCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCEECCCCCCE
EVPVWGEISIVVEQLKTALSMQVDTEDVQSEIAERWAIWRAEKARRELDDRGQGLNSAAI
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
FAAMTRWVPAHAVIAVDVGNNTYSFGRYFECQAQSVLMSGYLGSIGFAFPAAMGAWAAAG
HHHHHHCCCCEEEEEEECCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HKRPIVSVSGDGGFGQYLAEFTTAVKYQMNITHILLHNDQLGKISKEQRAGEWDVWQTSL
CCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCHHHCCCCCCHHHHCC
HNPDFSQYAQNCGGFGIRVTQPDELDDAMAQALAYQGPALVEIMSDPELI
CCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure 
PEITPQWFRVLDLDELPEGRVKTVVAGHKSLALTHHQGQYGALDNRCPHQGGPLGEGSI
CCCCHHHHEEECHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ENGLLRCPWHGWDYCPLTGQPPGGLDVDDALETFPVEVRDDGVYVGLPVEKPHIRTVSDV
CCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCEEEEECCCCCCCHHHHHH
MAETMVNWGVTHVFGMVGHSNLGLADTLRRQEEQGKLTYIGIRHEGAAAFAASAYAKLTG
HHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCC
TPAACLTIAGPGATNLLTGMWDAKVDCAPILALTGQVNSQVLGTGNFQECDLSAAFSGVA
CCCEEEEEECCCHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHH
AWSQTVLSHSKHAELMTLAIKHALLERNVSHLIFPDEIQVQPAVDVPAATPTGRITPLQI
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCCCCEEEEEE
APPPESLQQAIGKLKQSRRPVIIVGHGARFHMPAVIELAELLGAPVLTTFKGKGQIADDH
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCC
PLGCGVLGRSGTPIASWFMNEADVLLVLGASFSNHTGIYPGKPIIQVDFDPLALGKFHPI
CCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCEECCCCCCE
EVPVWGEISIVVEQLKTALSMQVDTEDVQSEIAERWAIWRAEKARRELDDRGQGLNSAAI
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
FAAMTRWVPAHAVIAVDVGNNTYSFGRYFECQAQSVLMSGYLGSIGFAFPAAMGAWAAAG
HHHHHHCCCCEEEEEEECCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HKRPIVSVSGDGGFGQYLAEFTTAVKYQMNITHILLHNDQLGKISKEQRAGEWDVWQTSL
CCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCHHHCCCCCCHHHHCC
HNPDFSQYAQNCGGFGIRVTQPDELDDAMAQALAYQGPALVEIMSDPELI
CCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate

Specific reaction: 2 pyruvate = 2-acetolactate + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]