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The map label for this gene is ykoW [H]

Identifier: 158336421

GI number: 158336421

Start: 3317115

End: 3318737

Strand: Direct

Name: ykoW [H]

Synonym: AM1_3285

Alternate gene names: 158336421

Gene position: 3317115-3318737 (Clockwise)

Preceding gene: 158336420

Following gene: 158336429

Centisome position: 51.0

GC content: 49.23

Gene sequence:

>1623_bases
ATGCGTCGGCAAGGGGTCACGGCGGATGAAGTCCCTAATCTGTCCGGCTGGACCAACTTTGTCTTAGCGGTCAATCAAAC
CATCCAAGAGTTCACTGCCAACCGCGAACTGTTAGAACAGTCCGTCCAGTCAACATCGCTCAAACTCAAGCAGGCCTACG
AAGATCTCAAGAGTGAATCTAGTTTACGGTTAGCCCAGGCAGACTTGCATCAGCGGGAACTAGAAAACCTAGTGCAAGAA
AGAACTGCTGAGCTACAGGCCGCCCAGCTCCATCTCGAACAAATCAATAAGCGCTTGGAATATGACGCTACCCATGATGA
CTTAACGGGGTTGGCAAACCGTAACCAATTAATGCGGGAGTTAAATCGATGTGTGGCTCAGCTCGGCACGGATTTAGCTG
TAGGACTGTGCTTTTTCTTTATCGACTTTGATCGCTTTAAGCAAATTAACGACCGGTTTGGTCACCTTATGGGTGACAAA
GTGTTGATTCAGGTTGCCAATCGCCTGTCCACGTTGGCAGGTGATGCAACCTGCCTGGCCCGGTTGGGGGGAGACGAATT
CGTGTTGCTAACGGGGCAACTGACGGTCGCTGAAGCCTACAAACTGGCCCAGCGAATCACGGCTGCCTTTGCTCAACCCC
TGCGCATTCGCGAGACCGAAATTTCCATGTCGGCAAGTGTTGGCATCGTCTTAGGGGATGCATCCTATCAATCTGCTGAT
GACGTCGTTCGTGATGCTGATATTGCCATGTATCGCGCTAAAGCAACGGGAAATTGCTATCAATTCTTCGATGAAAGAAT
GCGGACAGAGTATTTAGAAGGCTTGGAACTAGAACGAGAATTAGAAACCGCTGTGCGGGAGAAACAGTTCCGCGTCAATT
TTGAACCCATTGTTGATATCCATCAGTCCGACATCTACTCGATTGAGTCGCTAGTGCGCTGGATTCATCCCGACAAGGGC
ATAATCTCGCCCGGTCGCTTTATTTCCCTGGCAGAAGAATTAGGGCTCGTGATCGATATCGACCGAATTGTTTTCGAGCA
AACCTGTAAACAGTTTCGTCAATGGCGGGCTAGCGGCATCACCCGTCCTCAGCAGAAGTTTAATGTCAATCTGTCCTGTG
GCCAGCTGGAACGATCCGATATTATCCCCTTCCTCCTCAGCACCATTGATGTATCCGGTATTAGCCCCCACGACGTCGTA
CTGGAAATTACGGAGAGTTACTTGCTGGCAGACTCTGTACTGGTGATGAAGAATTTAAATGAAATGAACGAGCTGGGATT
TAGCATTTTTGTGGATGACTTTGGGACGGGATACTCATCCCTGAGTTATCTCGCCAAGTATCCCATCCACGGCATTAAGA
TTGATCGGAGTTTTGTCAGAGATCTGACAGAAAGCCCAGAAAGCAAAGAGTTAATTCGCTCCATGATTGCCATGGCTGAT
GCATTGAATCTGCAAGTGGTGACTGAAGGGGTAGAAACCCTAGAGCAATTGCAGATTATGACGGAACTGGGCTGCCGCTA
TATTCAAGGCTTCTTATTTACGCGGCCGATGGATGCCATGCAGGCCGAAACGTTTTTAGTGGATCAGCCGTATCGAGAGG
TAGGTGCGATCGCACCCATGTAA

Upstream 100 bases:

>100_bases
CACGATGGGGCTAATACGAGAGTATTAGGGATTGACCATGGCGATTCAACCTGTTCCCGACCATCGGTCTAGCGCACTCA
GCCGCATGCTTAAACGGCTA

Downstream 100 bases:

>100_bases
CACGAACCTAAAGGCTTCACACAGGCCATGAGAGAGGTGTTTAAGACTTGTGCAGGCTAGGCCTTAAGAAGAGACTTTTT
CCTCAGCAACGGCTCCTTCA

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MRRQGVTADEVPNLSGWTNFVLAVNQTIQEFTANRELLEQSVQSTSLKLKQAYEDLKSESSLRLAQADLHQRELENLVQE
RTAELQAAQLHLEQINKRLEYDATHDDLTGLANRNQLMRELNRCVAQLGTDLAVGLCFFFIDFDRFKQINDRFGHLMGDK
VLIQVANRLSTLAGDATCLARLGGDEFVLLTGQLTVAEAYKLAQRITAAFAQPLRIRETEISMSASVGIVLGDASYQSAD
DVVRDADIAMYRAKATGNCYQFFDERMRTEYLEGLELERELETAVREKQFRVNFEPIVDIHQSDIYSIESLVRWIHPDKG
IISPGRFISLAEELGLVIDIDRIVFEQTCKQFRQWRASGITRPQQKFNVNLSCGQLERSDIIPFLLSTIDVSGISPHDVV
LEITESYLLADSVLVMKNLNEMNELGFSIFVDDFGTGYSSLSYLAKYPIHGIKIDRSFVRDLTESPESKELIRSMIAMAD
ALNLQVVTEGVETLEQLQIMTELGCRYIQGFLFTRPMDAMQAETFLVDQPYREVGAIAPM

Sequences:

>Translated_540_residues
MRRQGVTADEVPNLSGWTNFVLAVNQTIQEFTANRELLEQSVQSTSLKLKQAYEDLKSESSLRLAQADLHQRELENLVQE
RTAELQAAQLHLEQINKRLEYDATHDDLTGLANRNQLMRELNRCVAQLGTDLAVGLCFFFIDFDRFKQINDRFGHLMGDK
VLIQVANRLSTLAGDATCLARLGGDEFVLLTGQLTVAEAYKLAQRITAAFAQPLRIRETEISMSASVGIVLGDASYQSAD
DVVRDADIAMYRAKATGNCYQFFDERMRTEYLEGLELERELETAVREKQFRVNFEPIVDIHQSDIYSIESLVRWIHPDKG
IISPGRFISLAEELGLVIDIDRIVFEQTCKQFRQWRASGITRPQQKFNVNLSCGQLERSDIIPFLLSTIDVSGISPHDVV
LEITESYLLADSVLVMKNLNEMNELGFSIFVDDFGTGYSSLSYLAKYPIHGIKIDRSFVRDLTESPESKELIRSMIAMAD
ALNLQVVTEGVETLEQLQIMTELGCRYIQGFLFTRPMDAMQAETFLVDQPYREVGAIAPM
>Mature_540_residues
MRRQGVTADEVPNLSGWTNFVLAVNQTIQEFTANRELLEQSVQSTSLKLKQAYEDLKSESSLRLAQADLHQRELENLVQE
RTAELQAAQLHLEQINKRLEYDATHDDLTGLANRNQLMRELNRCVAQLGTDLAVGLCFFFIDFDRFKQINDRFGHLMGDK
VLIQVANRLSTLAGDATCLARLGGDEFVLLTGQLTVAEAYKLAQRITAAFAQPLRIRETEISMSASVGIVLGDASYQSAD
DVVRDADIAMYRAKATGNCYQFFDERMRTEYLEGLELERELETAVREKQFRVNFEPIVDIHQSDIYSIESLVRWIHPDKG
IISPGRFISLAEELGLVIDIDRIVFEQTCKQFRQWRASGITRPQQKFNVNLSCGQLERSDIIPFLLSTIDVSGISPHDVV
LEITESYLLADSVLVMKNLNEMNELGFSIFVDDFGTGYSSLSYLAKYPIHGIKIDRSFVRDLTESPESKELIRSMIAMAD
ALNLQVVTEGVETLEQLQIMTELGCRYIQGFLFTRPMDAMQAETFLVDQPYREVGAIAPM

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=430, Percent_Identity=35.8139534883721, Blast_Score=265, Evalue=7e-72,
Organism=Escherichia coli, GI87081921, Length=457, Percent_Identity=33.9168490153173, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI226510982, Length=463, Percent_Identity=27.8617710583153, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081743, Length=253, Percent_Identity=34.7826086956522, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788502, Length=319, Percent_Identity=29.7805642633229, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081845, Length=258, Percent_Identity=31.7829457364341, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790496, Length=269, Percent_Identity=29.368029739777, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787055, Length=440, Percent_Identity=23.4090909090909, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081980, Length=272, Percent_Identity=28.3088235294118, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1786507, Length=239, Percent_Identity=28.4518828451883, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082096, Length=434, Percent_Identity=26.4976958525346, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=38.8888888888889, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788849, Length=250, Percent_Identity=28.8, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081977, Length=164, Percent_Identity=37.8048780487805, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=34.1040462427746, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=33.9393939393939, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=29.8245614035088, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=30.9090909090909, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787816, Length=187, Percent_Identity=31.0160427807487, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=29.6511627906977, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787410, Length=136, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788085, Length=161, Percent_Identity=29.8136645962733, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI145693134, Length=188, Percent_Identity=30.8510638297872, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789650, Length=446, Percent_Identity=20.8520179372197, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 61116; Mature: 61116

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRQGVTADEVPNLSGWTNFVLAVNQTIQEFTANRELLEQSVQSTSLKLKQAYEDLKSES
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SLRLAQADLHQRELENLVQERTAELQAAQLHLEQINKRLEYDATHDDLTGLANRNQLMRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LNRCVAQLGTDLAVGLCFFFIDFDRFKQINDRFGHLMGDKVLIQVANRLSTLAGDATCLA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RLGGDEFVLLTGQLTVAEAYKLAQRITAAFAQPLRIRETEISMSASVGIVLGDASYQSAD
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCEEEEEECCCCCCHH
DVVRDADIAMYRAKATGNCYQFFDERMRTEYLEGLELERELETAVREKQFRVNFEPIVDI
HHHHHCHHHHEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHC
HQSDIYSIESLVRWIHPDKGIISPGRFISLAEELGLVIDIDRIVFEQTCKQFRQWRASGI
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCC
TRPQQKFNVNLSCGQLERSDIIPFLLSTIDVSGISPHDVVLEITESYLLADSVLVMKNLN
CCCHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMNELGFSIFVDDFGTGYSSLSYLAKYPIHGIKIDRSFVRDLTESPESKELIRSMIAMAD
HHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHHH
ALNLQVVTEGVETLEQLQIMTELGCRYIQGFLFTRPMDAMQAETFLVDQPYREVGAIAPM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCHHHHCCCCCC
>Mature Secondary Structure
MRRQGVTADEVPNLSGWTNFVLAVNQTIQEFTANRELLEQSVQSTSLKLKQAYEDLKSES
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SLRLAQADLHQRELENLVQERTAELQAAQLHLEQINKRLEYDATHDDLTGLANRNQLMRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LNRCVAQLGTDLAVGLCFFFIDFDRFKQINDRFGHLMGDKVLIQVANRLSTLAGDATCLA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RLGGDEFVLLTGQLTVAEAYKLAQRITAAFAQPLRIRETEISMSASVGIVLGDASYQSAD
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCEEEEEECCCCCCHH
DVVRDADIAMYRAKATGNCYQFFDERMRTEYLEGLELERELETAVREKQFRVNFEPIVDI
HHHHHCHHHHEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHC
HQSDIYSIESLVRWIHPDKGIISPGRFISLAEELGLVIDIDRIVFEQTCKQFRQWRASGI
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCC
TRPQQKFNVNLSCGQLERSDIIPFLLSTIDVSGISPHDVVLEITESYLLADSVLVMKNLN
CCCHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMNELGFSIFVDDFGTGYSSLSYLAKYPIHGIKIDRSFVRDLTESPESKELIRSMIAMAD
HHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHHH
ALNLQVVTEGVETLEQLQIMTELGCRYIQGFLFTRPMDAMQAETFLVDQPYREVGAIAPM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]