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The map label for this gene is rpoD [H]

Identifier: 158336372

GI number: 158336372

Start: 3271974

End: 3272930

Strand: Direct

Name: rpoD [H]

Synonym: AM1_3234

Alternate gene names: 158336372

Gene position: 3271974-3272930 (Clockwise)

Preceding gene: 158336371

Following gene: 158336373

Centisome position: 50.31

GC content: 46.71

Gene sequence:

>957_bases
ATGAAAAATTCATTTGTTAGTAAAGATTCAATCACTACCTACCTGCGTGAGATTAGCCGCTTTCCTCTGTTGAATCATGA
ACAAGAAGTCATCTTTGGCAAGCAGGTACAACGCCTAACAGCTTTGCAGGAGATGAAGCAGGAACTGCAAGATCACCTTG
ACCGAGAGCCGACCCAAAAAGAATGGGCAGAAAAAGCCCAAGTGTCTGTAGCGCAACTGCAAAAAGAATTGAAGTTAGGC
CACAAAGCCAATCGCAAAATGGTGGAATCTAATTTGCGCCTGGTGGTCTCAATTGCCAAGAAGTACAATAAAGCGAAAGT
TGAGTTTTTGGATTTGATCCAAGAAGGCACAATTGGTCTGCAACGGGGTGTCGAAAAATTTGACCCGATGAAAGGCTATC
GCTTCTCTACCTATGCCTACTGGTGGATTCGTCAAGCCATGACTCGTGCGATCGCAGAACAAGAACGAACCATCCGCTTA
CCGATTCATATCAATGAAAAACTCAGTAAGCTGCGCAAGGTCAGACGCCAACTGTCGCAAAAACTCCATCGCGACCCTAC
TGTGCAAGAGCTAGCGGTAGAGATGGACCTCGCGCCTAAAAAGATTGAAGACTATCTTAACTATGCGCGTCGCTCGATCT
CGTTAGATATTCAGATTGGTGACCAGCAAAATACAGAGCTATGGGAATTGCTAGAAGATCCTAATGAATCTCCCGATACC
TATGCTACGGCCTCTTCTCTGCGTTCGGATTTAGCCAAGCTGCTTCAAGACCTCAGCCCGCAGCAGCGGGAAGTGATTAC
TTTGCGATACGGCCTGATTGACCAAAATCCGCTGACCTTAGCAAAGGTGGGTGAACGTCTAAACGTCAGCCGGGAATGGG
TTCGCCGGGTTGAGCGGGAAGCCTTTAAGATTTTGCGATCGCAAAAAGTGATGCTGCAAGAATATATGGCTTGCTAA

Upstream 100 bases:

>100_bases
GGGTTTAGAACGACTCTAGTTCATCTTTAATATTTTTTCCCCGGCATGTGCCGGGGAGTTTTATTTATCAGTTATCTGTG
TTTACCCAAACATTGCCATT

Downstream 100 bases:

>100_bases
GCAACGATTTCCAATCTCAATCTGCGCATCAGCTACTCTTCTGCCTGCAAGAGTAGCTTTTTTGTAACAGAAAATTTACA
CTTTGGTAGCCTATCCTTTG

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKNSFVSKDSITTYLREISRFPLLNHEQEVIFGKQVQRLTALQEMKQELQDHLDREPTQKEWAEKAQVSVAQLQKELKLG
HKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGLQRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRL
PIHINEKLSKLRKVRRQLSQKLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
YATASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVEREAFKILRSQKVMLQEYMAC

Sequences:

>Translated_318_residues
MKNSFVSKDSITTYLREISRFPLLNHEQEVIFGKQVQRLTALQEMKQELQDHLDREPTQKEWAEKAQVSVAQLQKELKLG
HKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGLQRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRL
PIHINEKLSKLRKVRRQLSQKLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
YATASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVEREAFKILRSQKVMLQEYMAC
>Mature_318_residues
MKNSFVSKDSITTYLREISRFPLLNHEQEVIFGKQVQRLTALQEMKQELQDHLDREPTQKEWAEKAQVSVAQLQKELKLG
HKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGLQRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRL
PIHINEKLSKLRKVRRQLSQKLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
YATASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVEREAFKILRSQKVMLQEYMAC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=42.9752066115703, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1789098, Length=300, Percent_Identity=36.3333333333333, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=25.5144032921811, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788231, Length=214, Percent_Identity=28.9719626168224, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37367; Mature: 37367

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSFVSKDSITTYLREISRFPLLNHEQEVIFGKQVQRLTALQEMKQELQDHLDREPTQK
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EWAEKAQVSVAQLQKELKLGHKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
QRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRLPIHINEKLSKLRKVRRQLSQ
HHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHH
KLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCH
YATASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVERE
HHHHHHHHHHHHHHHHHCCCCHHHEEEHEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
AFKILRSQKVMLQEYMAC
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNSFVSKDSITTYLREISRFPLLNHEQEVIFGKQVQRLTALQEMKQELQDHLDREPTQK
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EWAEKAQVSVAQLQKELKLGHKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
QRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRLPIHINEKLSKLRKVRRQLSQ
HHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHH
KLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCH
YATASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVERE
HHHHHHHHHHHHHHHHHCCCCHHHEEEHEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
AFKILRSQKVMLQEYMAC
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA