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The map label for this gene is groEL

Identifier: 158336352

GI number: 158336352

Start: 3252289

End: 3253965

Strand: Direct

Name: groEL

Synonym: AM1_3214

Alternate gene names: 158336352

Gene position: 3252289-3253965 (Clockwise)

Preceding gene: 158336351

Following gene: 158336353

Centisome position: 50.01

GC content: 51.58

Gene sequence:

>1677_bases
ATGGCCAAACATGTAGTATTTGATGAAGAATCGCGTCGCGCTCTTGAGCGTGGTGTGAATTCTCTGGCAGATGCCGTACG
AATTACATTGGGACCTAAAGGTCGCAATGTGGTTCTGGAAAAGAAATTCGGAGCACCCCAAATTATTAATGATGGGGTGA
CCATTGCGAAGGAAGTTGAGCTGGAAGATCCGTTGGAGAATGCGGGTGCACAGCTGATGCGTGAAGTCGCTTCCAAAACC
AATGATGTCGCGGGTGATGGTACCACTACCGCTACAGTTCTGGCCCAAGCTTTGATTCGCGAAGGTTTGAAGAATGTGGC
TGCTGGTGCTAACCCCGTGGCCCTTCGCAAAGGGATTGATAAGACGATTGATGCCTTAGTGAAAGAGATCGAAGCCAAGT
CGAAGCCCGTTGCCGGAGATGCGATCGCACAAGTGGCAACCATCTCTGCCGGGAACGATACCGAAGTCGGTCAAATGATT
GCCCAAGCCATGGATAAGGTGGGCAAAGATGGCGTCATTACCGTAGAAGAATCCAAGTCCCTCGCCACCGAAATGGAAAT
TGTGGAAGGGATGCAAATCGATCGGGGTTATATCTCTCCTTACTTTGTGACTGATGCAGAACGCATGGTTGCTGAGATCG
AAAATGCCCGCTTGCTGATCGTTAACAAGAAAATCAGTTCTCTTCAAGATCTCGTCGGTATCCTGGAGCAAGTTGCCCGT
GCTGGACAGCCTCTCCTAATTATTGCTGAAGATCTAGAAGGAGAAGCCCTAGCCACGTTGGTGGTGAACAAGCTGCGGGG
TGTCCTTAATGTTGTGGCTATCAAGGCTCCTGGTTTTGGTGAGCGCCGCCAAGCCATGCTTCAAGACATTGCCGTCCTCA
CCGGTGGACAGGTCATTTCTGAAGATGTGGGTTTAACCCTCGACAAAGTTGATTTAGAGATGTTGGGGACGGCTCGTAAG
GTCACCATCAGCAAAGACAACACCACGATTGTGTCGGAAGCAGCCAATGCAGGTGATGTCGGCAAGCGAGTAGAGCAGCT
CCGTCGCCAGTTGGATGAAACCGATTCTGAGTACGACAAAGAAAAGCTGCAAGAGCGGATTGCTAAGCTGGCAGGGGGCG
TTGCTGTCATCAAAGTAGGTGCTGCAACCGAAACCGAGCTTAAGGATCGTAAATTGCGCATCGAAGATGCGCTCAATGCA
ACGAAAGCAGCCGTAGCTGAAGGGATCGTTCCTGGCGGTGGCACCACCCTGATTCATCTCACCAAAACCATTGAGAGTGT
TAAAGCTCAATTGAAAGATGAAGAAAAAGTCGGTGCAGATCTCGTGGGTATTGCGTTAGAAGCGCCTCTCACTCAAATTG
CAGACAATGCTGGTAAAGAAGGCGCTGTCGTTGTAGAGAAAGTCCGTGATGCGGACTTCAGCTATGGCTATAACGCCATG
ACTGACACCTACGAAGATATGATTGCTGCCGGAGTCGTTGATCCTGCAAAGGTGGTTCGTTCGGGCCTCCAAAATGCGGG
GTCGATTGCTGGCATGGTGCTCACCACTGAAGCCTTAGTAGTGGATAAGCCTGAACCTGCAGGTGCTGCTGCGCCCGATA
TGGGCGGCATGGGCGGCATGGGTGGCATGGGCGGCATGGGCGGCATGGGTGGCATGGGCGGCATGGGCATGATGTAA

Upstream 100 bases:

>100_bases
GTTAGCTTTAGCACTCTCCTCTTGAGAGTGCTAGTGTGCTGACAAACATGAAACGTTGTGTGTAATTCACTGAGAAGGCG
CAAACGAGAGGACGCTTGTA

Downstream 100 bases:

>100_bases
GCCCAAGCACCTATGACTCGTCAGCACTAAGTTAGCTCTGAGTCTTGCAAAACAGCTTGTCATCTGACAGGCTGTTTTTT
GGTGTATGAGCAGTCATGCT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 1; Protein Cpn60 1

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MAKHVVFDEESRRALERGVNSLADAVRITLGPKGRNVVLEKKFGAPQIINDGVTIAKEVELEDPLENAGAQLMREVASKT
NDVAGDGTTTATVLAQALIREGLKNVAAGANPVALRKGIDKTIDALVKEIEAKSKPVAGDAIAQVATISAGNDTEVGQMI
AQAMDKVGKDGVITVEESKSLATEMEIVEGMQIDRGYISPYFVTDAERMVAEIENARLLIVNKKISSLQDLVGILEQVAR
AGQPLLIIAEDLEGEALATLVVNKLRGVLNVVAIKAPGFGERRQAMLQDIAVLTGGQVISEDVGLTLDKVDLEMLGTARK
VTISKDNTTIVSEAANAGDVGKRVEQLRRQLDETDSEYDKEKLQERIAKLAGGVAVIKVGAATETELKDRKLRIEDALNA
TKAAVAEGIVPGGGTTLIHLTKTIESVKAQLKDEEKVGADLVGIALEAPLTQIADNAGKEGAVVVEKVRDADFSYGYNAM
TDTYEDMIAAGVVDPAKVVRSGLQNAGSIAGMVLTTEALVVDKPEPAGAAAPDMGGMGGMGGMGGMGGMGGMGGMGMM

Sequences:

>Translated_558_residues
MAKHVVFDEESRRALERGVNSLADAVRITLGPKGRNVVLEKKFGAPQIINDGVTIAKEVELEDPLENAGAQLMREVASKT
NDVAGDGTTTATVLAQALIREGLKNVAAGANPVALRKGIDKTIDALVKEIEAKSKPVAGDAIAQVATISAGNDTEVGQMI
AQAMDKVGKDGVITVEESKSLATEMEIVEGMQIDRGYISPYFVTDAERMVAEIENARLLIVNKKISSLQDLVGILEQVAR
AGQPLLIIAEDLEGEALATLVVNKLRGVLNVVAIKAPGFGERRQAMLQDIAVLTGGQVISEDVGLTLDKVDLEMLGTARK
VTISKDNTTIVSEAANAGDVGKRVEQLRRQLDETDSEYDKEKLQERIAKLAGGVAVIKVGAATETELKDRKLRIEDALNA
TKAAVAEGIVPGGGTTLIHLTKTIESVKAQLKDEEKVGADLVGIALEAPLTQIADNAGKEGAVVVEKVRDADFSYGYNAM
TDTYEDMIAAGVVDPAKVVRSGLQNAGSIAGMVLTTEALVVDKPEPAGAAAPDMGGMGGMGGMGGMGGMGGMGGMGMM
>Mature_557_residues
AKHVVFDEESRRALERGVNSLADAVRITLGPKGRNVVLEKKFGAPQIINDGVTIAKEVELEDPLENAGAQLMREVASKTN
DVAGDGTTTATVLAQALIREGLKNVAAGANPVALRKGIDKTIDALVKEIEAKSKPVAGDAIAQVATISAGNDTEVGQMIA
QAMDKVGKDGVITVEESKSLATEMEIVEGMQIDRGYISPYFVTDAERMVAEIENARLLIVNKKISSLQDLVGILEQVARA
GQPLLIIAEDLEGEALATLVVNKLRGVLNVVAIKAPGFGERRQAMLQDIAVLTGGQVISEDVGLTLDKVDLEMLGTARKV
TISKDNTTIVSEAANAGDVGKRVEQLRRQLDETDSEYDKEKLQERIAKLAGGVAVIKVGAATETELKDRKLRIEDALNAT
KAAVAEGIVPGGGTTLIHLTKTIESVKAQLKDEEKVGADLVGIALEAPLTQIADNAGKEGAVVVEKVRDADFSYGYNAMT
DTYEDMIAAGVVDPAKVVRSGLQNAGSIAGMVLTTEALVVDKPEPAGAAAPDMGGMGGMGGMGGMGGMGGMGGMGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=46.0227272727273, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=46.0227272727273, Blast_Score=467, Evalue=1e-131,
Organism=Escherichia coli, GI1790586, Length=525, Percent_Identity=57.9047619047619, Blast_Score=594, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=46.0227272727273, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI193210679, Length=214, Percent_Identity=40.6542056074766, Blast_Score=172, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323288, Length=525, Percent_Identity=49.1428571428571, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=47.2485768500949, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=47.2485768500949, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=46.0952380952381, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=46.0952380952381, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=46.0952380952381, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864606, Length=524, Percent_Identity=41.6030534351145, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24584129, Length=549, Percent_Identity=33.6976320582878, Blast_Score=287, Evalue=2e-77,
Organism=Drosophila melanogaster, GI19921262, Length=549, Percent_Identity=33.6976320582878, Blast_Score=287, Evalue=2e-77,
Organism=Drosophila melanogaster, GI17647245, Length=540, Percent_Identity=21.8518518518519, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652903, Length=540, Percent_Identity=21.8518518518519, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH601_ACAM1 (B0CFQ6)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517526.1
- ProteinModelPortal:   B0CFQ6
- SMR:   B0CFQ6
- GeneID:   5682022
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3214
- HOGENOM:   HBG625289
- OMA:   VLITDQK
- ProtClustDB:   PRK12849
- BioCyc:   AMAR329726:AM1_3214-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58389; Mature: 58258

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHVVFDEESRRALERGVNSLADAVRITLGPKGRNVVLEKKFGAPQIINDGVTIAKEVE
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCEECCCCEEEEECC
LEDPLENAGAQLMREVASKTNDVAGDGTTTATVLAQALIREGLKNVAAGANPVALRKGID
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHH
KTIDALVKEIEAKSKPVAGDAIAQVATISAGNDTEVGQMIAQAMDKVGKDGVITVEESKS
HHHHHHHHHHHHCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCHH
LATEMEIVEGMQIDRGYISPYFVTDAERMVAEIENARLLIVNKKISSLQDLVGILEQVAR
HHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
AGQPLLIIAEDLEGEALATLVVNKLRGVLNVVAIKAPGFGERRQAMLQDIAVLTGGQVIS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
EDVGLTLDKVDLEMLGTARKVTISKDNTTIVSEAANAGDVGKRVEQLRRQLDETDSEYDK
HCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHH
EKLQERIAKLAGGVAVIKVGAATETELKDRKLRIEDALNATKAAVAEGIVPGGGTTLIHL
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHH
TKTIESVKAQLKDEEKVGADLVGIALEAPLTQIADNAGKEGAVVVEKVRDADFSYGYNAM
HHHHHHHHHHHCHHHHHCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHH
TDTYEDMIAAGVVDPAKVVRSGLQNAGSIAGMVLTTEALVVDKPEPAGAAAPDMGGMGGM
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCC
GGMGGMGGMGGMGGMGMM
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKHVVFDEESRRALERGVNSLADAVRITLGPKGRNVVLEKKFGAPQIINDGVTIAKEVE
CCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCEECCCCEEEEECC
LEDPLENAGAQLMREVASKTNDVAGDGTTTATVLAQALIREGLKNVAAGANPVALRKGID
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHH
KTIDALVKEIEAKSKPVAGDAIAQVATISAGNDTEVGQMIAQAMDKVGKDGVITVEESKS
HHHHHHHHHHHHCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCHH
LATEMEIVEGMQIDRGYISPYFVTDAERMVAEIENARLLIVNKKISSLQDLVGILEQVAR
HHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
AGQPLLIIAEDLEGEALATLVVNKLRGVLNVVAIKAPGFGERRQAMLQDIAVLTGGQVIS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
EDVGLTLDKVDLEMLGTARKVTISKDNTTIVSEAANAGDVGKRVEQLRRQLDETDSEYDK
HCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHH
EKLQERIAKLAGGVAVIKVGAATETELKDRKLRIEDALNATKAAVAEGIVPGGGTTLIHL
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHH
TKTIESVKAQLKDEEKVGADLVGIALEAPLTQIADNAGKEGAVVVEKVRDADFSYGYNAM
HHHHHHHHHHHCHHHHHCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHH
TDTYEDMIAAGVVDPAKVVRSGLQNAGSIAGMVLTTEALVVDKPEPAGAAAPDMGGMGGM
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCC
GGMGGMGGMGGMGGMGMM
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA