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The map label for this gene is ntcA [H]

Identifier: 158336293

GI number: 158336293

Start: 3184664

End: 3185353

Strand: Direct

Name: ntcA [H]

Synonym: AM1_3155

Alternate gene names: 158336293

Gene position: 3184664-3185353 (Clockwise)

Preceding gene: 158336289

Following gene: 158336297

Centisome position: 48.97

GC content: 48.55

Gene sequence:

>690_bases
ATGGCCTCGCCTCAAGCTAATCGATTAACCCCCGAAAGTCCTCTTGCAGCTGTTTTTCGGCAGATGGGGAGTGGACTATT
TCCTCCCGTCACCGAAACCTTTGATCGAGGAAAAACGATTTTTTTCCCAGGTGATCCGGCAGAGCGTGTTTACTTTTTAA
TTAAAGGAGCAGTTAAGCTTTCCCGCGTTTATGAAGCGGGTGAAGAAATTACCGTCGCACTGCTCCGGGAAAATAGCGTC
TTTGGCGTTTTATCGCTACTGACGAAGCAGCGCTCTGATCGCTTTTACCATGCGGTTGCCTTTACGCCCGTTGAACTCCT
CTCTGTGCCCATTGAGCAGGTGGAAAAAGCTCTGCAAAGTGATCCTGACTTACCCATGGTGCTGCTGCGGGGCTTGTCAT
CTCGGATTATTCAAACCGAGATGATGATTGAAACCTTGGCTCACCGGGATATGGAATCTCGCTTAATCAGTTTTCTGCTC
ATTCTCTGCCGTGATTTTGGGATTCCCAATGGTAGCGGCATTACCGTAGACCTAAAATTGTCTCACCAAGCGATCGCAGA
AGCGATTGGCTCTACCCGAGTGACTGTGACTCGCTTACTTGGGGATCTACGAGATCAGGAAAAAATTTCCATTCATAAGA
AGAAGATCACGGTTCACGATCCGGTAATGCTCGGTCAGAGCTTTGCCTAG

Upstream 100 bases:

>100_bases
GACTTTAATTTCACCGTTTTACCTCAGACATAAACACTAATTTTTCTTGCTTGTCTCTTCCTATACATCTTCCGTATCGA
GTCAGGGTATGAGTATGTTA

Downstream 100 bases:

>100_bases
GGCTATTTGTCTATATCCTTAGGGTTTGTAAGGACGGTTGGATAAACCGAGAATTTGTCGCTCCTTCTGGGCCGCTACGG
CAGTAGTTTGGGCCGGATTG

Product: nitrogen-responsive regulatory protein

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MASPQANRLTPESPLAAVFRQMGSGLFPPVTETFDRGKTIFFPGDPAERVYFLIKGAVKLSRVYEAGEEITVALLRENSV
FGVLSLLTKQRSDRFYHAVAFTPVELLSVPIEQVEKALQSDPDLPMVLLRGLSSRIIQTEMMIETLAHRDMESRLISFLL
ILCRDFGIPNGSGITVDLKLSHQAIAEAIGSTRVTVTRLLGDLRDQEKISIHKKKITVHDPVMLGQSFA

Sequences:

>Translated_229_residues
MASPQANRLTPESPLAAVFRQMGSGLFPPVTETFDRGKTIFFPGDPAERVYFLIKGAVKLSRVYEAGEEITVALLRENSV
FGVLSLLTKQRSDRFYHAVAFTPVELLSVPIEQVEKALQSDPDLPMVLLRGLSSRIIQTEMMIETLAHRDMESRLISFLL
ILCRDFGIPNGSGITVDLKLSHQAIAEAIGSTRVTVTRLLGDLRDQEKISIHKKKITVHDPVMLGQSFA
>Mature_228_residues
ASPQANRLTPESPLAAVFRQMGSGLFPPVTETFDRGKTIFFPGDPAERVYFLIKGAVKLSRVYEAGEEITVALLRENSVF
GVLSLLTKQRSDRFYHAVAFTPVELLSVPIEQVEKALQSDPDLPMVLLRGLSSRIIQTEMMIETLAHRDMESRLISFLLI
LCRDFGIPNGSGITVDLKLSHQAIAEAIGSTRVTVTRLLGDLRDQEKISIHKKKITVHDPVMLGQSFA

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=184, Percent_Identity=28.804347826087, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25405; Mature: 25274

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPQANRLTPESPLAAVFRQMGSGLFPPVTETFDRGKTIFFPGDPAERVYFLIKGAVKL
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
SRVYEAGEEITVALLRENSVFGVLSLLTKQRSDRFYHAVAFTPVELLSVPIEQVEKALQS
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEHHHHCHHHHHHCCHHHHHHHHHC
DPDLPMVLLRGLSSRIIQTEMMIETLAHRDMESRLISFLLILCRDFGIPNGSGITVDLKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
SHQAIAEAIGSTRVTVTRLLGDLRDQEKISIHKKKITVHDPVMLGQSFA
CHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCCHHCCCCCC
>Mature Secondary Structure 
ASPQANRLTPESPLAAVFRQMGSGLFPPVTETFDRGKTIFFPGDPAERVYFLIKGAVKL
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
SRVYEAGEEITVALLRENSVFGVLSLLTKQRSDRFYHAVAFTPVELLSVPIEQVEKALQS
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEHHHHCHHHHHHCCHHHHHHHHHC
DPDLPMVLLRGLSSRIIQTEMMIETLAHRDMESRLISFLLILCRDFGIPNGSGITVDLKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
SHQAIAEAIGSTRVTVTRLLGDLRDQEKISIHKKKITVHDPVMLGQSFA
CHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]