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The map label for this gene is fabI [H]

Identifier: 158336292

GI number: 158336292

Start: 3183533

End: 3184309

Strand: Reverse

Name: fabI [H]

Synonym: AM1_3154

Alternate gene names: 158336292

Gene position: 3184309-3183533 (Counterclockwise)

Preceding gene: 158336294

Following gene: 158336291

Centisome position: 48.96

GC content: 49.03

Gene sequence:

>777_bases
ATGCTTGATTTAACTGGAAAAAACGCCCTGGTCACTGGAATTGCCAATAACCGGTCCATTGCCTGGGGCATTGCCCAGCA
ACTCCACAGCGCCGGGGCCAATCTGGGGATTACTTATCTACCCGATGAGAAAGGACGGAACGAGTCCAAAGTTAAAAAGC
TGGTTGAACCTCTAGCGCCCAGCTTGTTTTTACCTTGTAATGTTCAAGATCAAGCGCAAGTAGATGCCACGTTCAGTTCG
ATTCAGCAGCAGTGGGGCAAGTTAGATATCTTGATTCATTGCTTGGCTTTTGCCAACCGAGAAGATTTGGGCGGTGATTT
TAGTGCGGTCCAACAAGACAATTTCAACCTGGCTTTGGATGTCAGTGCTTACTCCTTAGTGCAATTGAGCGCTGCCGCTA
AACCGCTGATGACGGAAGGGGGCAGCATTGTGACCTTGACTTACTTAGGCGGTGCCCGAGTCGTGCCTAATTACAACGTT
ATGGGTATCGCCAAAGCGGCATTAGAAATGAATGTTCGCTATTTAGCGGCAGAAATGGGTCCTCAAAACGTTCGCGTCAA
TGGCATTTCAGCTGGACCCATCCGAACCTTGGCCTCCTCTGCTGTGGGCGGCATTTTAGATATGATTCACCATGTTGAAG
AAGTGGCTCCCTTGAAGCGAACGGTCACCCAAACCGAAGTAGGCAACACTGCAGCCTTCCTCTGTAGTGATTTATCGACT
GGCATTACCGGGCAAATTCTCTATGTCGACTCAGGCTATTCGATTATGGGCATGTAA

Upstream 100 bases:

>100_bases
TGCTCACATCTCGTTCTAAGCTAGAACTGGAGTCAAGGTTCAGGCTTTTGTTGACCGCCTTCTTCCCCTGGCTTGTCATA
CTTCTATTCAACTGATTTTC

Downstream 100 bases:

>100_bases
AGCCTGGGTCATCCATTAAATAGGTCACTTCTGACACGCCTGAACTAGACTAGAACTCTAATCCTGAGATGACTAGCCGC
CTGATTCCCACCCTATGAAG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLDLTGKNALVTGIANNRSIAWGIAQQLHSAGANLGITYLPDEKGRNESKVKKLVEPLAPSLFLPCNVQDQAQVDATFSS
IQQQWGKLDILIHCLAFANREDLGGDFSAVQQDNFNLALDVSAYSLVQLSAAAKPLMTEGGSIVTLTYLGGARVVPNYNV
MGIAKAALEMNVRYLAAEMGPQNVRVNGISAGPIRTLASSAVGGILDMIHHVEEVAPLKRTVTQTEVGNTAAFLCSDLST
GITGQILYVDSGYSIMGM

Sequences:

>Translated_258_residues
MLDLTGKNALVTGIANNRSIAWGIAQQLHSAGANLGITYLPDEKGRNESKVKKLVEPLAPSLFLPCNVQDQAQVDATFSS
IQQQWGKLDILIHCLAFANREDLGGDFSAVQQDNFNLALDVSAYSLVQLSAAAKPLMTEGGSIVTLTYLGGARVVPNYNV
MGIAKAALEMNVRYLAAEMGPQNVRVNGISAGPIRTLASSAVGGILDMIHHVEEVAPLKRTVTQTEVGNTAAFLCSDLST
GITGQILYVDSGYSIMGM
>Mature_258_residues
MLDLTGKNALVTGIANNRSIAWGIAQQLHSAGANLGITYLPDEKGRNESKVKKLVEPLAPSLFLPCNVQDQAQVDATFSS
IQQQWGKLDILIHCLAFANREDLGGDFSAVQQDNFNLALDVSAYSLVQLSAAAKPLMTEGGSIVTLTYLGGARVVPNYNV
MGIAKAALEMNVRYLAAEMGPQNVRVNGISAGPIRTLASSAVGGILDMIHHVEEVAPLKRTVTQTEVGNTAAFLCSDLST
GITGQILYVDSGYSIMGM

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=198, Percent_Identity=30.8080808080808, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI7705925, Length=256, Percent_Identity=27.34375, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=48.2490272373541, Blast_Score=257, Evalue=6e-70,
Organism=Escherichia coli, GI1789378, Length=244, Percent_Identity=25.8196721311475, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082100, Length=268, Percent_Identity=26.865671641791, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790717, Length=258, Percent_Identity=25.968992248062, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=26.2948207171315, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787905, Length=259, Percent_Identity=29.3436293436293, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787335, Length=258, Percent_Identity=27.906976744186, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87082160, Length=260, Percent_Identity=25.7692307692308, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=280, Percent_Identity=30.3571428571429, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=280, Percent_Identity=29.2857142857143, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=281, Percent_Identity=29.1814946619217, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=278, Percent_Identity=26.6187050359712, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=191, Percent_Identity=29.3193717277487, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=275, Percent_Identity=28, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=114, Percent_Identity=35.9649122807018, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=275, Percent_Identity=26.5454545454545, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI21357041, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571526, Length=223, Percent_Identity=27.8026905829596, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=25.6809338521401, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644337, Length=120, Percent_Identity=35.8333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24643142, Length=263, Percent_Identity=25.4752851711027, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644339, Length=267, Percent_Identity=25.4681647940075, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27366; Mature: 27366

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLTGKNALVTGIANNRSIAWGIAQQLHSAGANLGITYLPDEKGRNESKVKKLVEPLAP
CCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCC
SLFLPCNVQDQAQVDATFSSIQQQWGKLDILIHCLAFANREDLGGDFSAVQQDNFNLALD
CEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEE
VSAYSLVQLSAAAKPLMTEGGSIVTLTYLGGARVVPNYNVMGIAKAALEMNVRYLAAEMG
ECHHHHHHHHHHCCHHHCCCCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHEEEECCC
PQNVRVNGISAGPIRTLASSAVGGILDMIHHVEEVAPLKRTVTQTEVGNTAAFLCSDLST
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
GITGQILYVDSGYSIMGM
CCCEEEEEEECCCEEECC
>Mature Secondary Structure
MLDLTGKNALVTGIANNRSIAWGIAQQLHSAGANLGITYLPDEKGRNESKVKKLVEPLAP
CCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCC
SLFLPCNVQDQAQVDATFSSIQQQWGKLDILIHCLAFANREDLGGDFSAVQQDNFNLALD
CEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEE
VSAYSLVQLSAAAKPLMTEGGSIVTLTYLGGARVVPNYNVMGIAKAALEMNVRYLAAEMG
ECHHHHHHHHHHCCHHHCCCCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHEEEECCC
PQNVRVNGISAGPIRTLASSAVGGILDMIHHVEEVAPLKRTVTQTEVGNTAAFLCSDLST
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
GITGQILYVDSGYSIMGM
CCCEEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]