Click here to switch to the map view.

The map label for this gene is dacB [C]

Identifier: 158336265

GI number: 158336265

Start: 3157082

End: 3158395

Strand: Reverse

Name: dacB [C]

Synonym: AM1_3127

Alternate gene names: 158336265

Gene position: 3158395-3157082 (Counterclockwise)

Preceding gene: 158336268

Following gene: 158336261

Centisome position: 48.56

GC content: 49.24

Gene sequence:

>1314_bases
ATGATCTGCCCCCCACGTCTTGCTTGTATATTCATCGCCCTCAGCCTGATCATCAGTGGTTGCTCTAACTCAGATATTCA
GCCCTCTTCTTCTCCTTCACCCGAGCCAACTTCTAGCGTTCCTGAACCCCCACCACCGCTCCAGCTAGGGCTAGCTGATC
CTGAAACCGTTGCTAATTCTCAGATCGAAACATACCTATCCCAGTTAGGCGCACCAACAGTGAGTCAAGGAGTCTGGATT
CAAACTCAAGACACCTTACTCGCCAACCATCAAGGCACGATTCCCTTACCGGCTGCCTCCATCACTAAAGTCGCTACCTC
CTTAGCCGTATTGAAGAAGTTAGGCCCCGATCACCGATTTGAAACCAAAATTGGCTATATCGGCACCCTTCAGAATGGAG
AACTAAACGGGGATTTGGTGATTGAGGGAGGCGCTGATCCGTTTTTTGTGTGGGAAGATGCGATCGCACTCGGTAACCTC
CTCAATCAAAACGGGATTCGCAACGTTAGCGGCAATTTGATTGTGGTTGGTCCGTTTTACATGAACTATGAACCCGACCC
CGTTACGTCTGGAACGTTGTTACAGCAGGGACTCGATCACACCCGCTGGCCTGCAGAAGCCCAAACGCAGTACCAAACCT
TGCCAGCTGATACGGCTAAACCTGAGGTTGCGATCGCAGGCAACATCCAAACTGCAGCCTCACGCCCTGATCAAGTCAAA
CCATTGATTCAGCATCGCTCTCTCCCTGTAGCTGAGCTGCTTAAAAAAATGAATCAGTACAGCAACAACGCCATGTCCGA
AATGCTAGCGGAATATGTGGGTGGTGCTACCCAGGTAGCTCAAATGGCAGCCCAAGAAGCCGGAGTTCCGTCTCAAGAAA
TTCAATTAATCAATGGTTCAGGTTTAGGGGTCGACAATCGTATCTCACCCCGAGCAGCCTGCGGCATGTTTCAGGCCATT
GCTCACCTATTGCACCTTCAAGAAATGACGATTGCAGATATTTTTGCTGTCATCGGCACTGATCCAGGGGTTCATAACGA
ACGCCCTCTCCCCCAAAAATTCGTGATGAAGTCGGGCACTTTGAATCGTGTCAGTTCCATTGCTGGAGCCTTGCCCACCA
AAAATCAAGGGGTCATCTGGTTCGCAGTCATTAACGGTGAAGGTAATGTGGACCAGTTCAGAGCTCAACAAGAAACTTTA
CTTCAGAACTTAATGCAGTCCTGGGGAACTACCAACAAGACTTTTGATCTGATCACGGCTAATCCTACACGACAAAATCT
GACGACTACTAGCAAAATAGTGCTCTCCAATTGA

Upstream 100 bases:

>100_bases
AAAGCCGTATCAGCCCCAATATAGATTGATCCATTCAATCTGAAAGAGATAACCAGATCATAAGATGTGGAATATTGCCG
ACTGCTAGTTTTGTCTCGTT

Downstream 100 bases:

>100_bases
GTCAGCAGCAAAGTATGCCACAGTTAACTACTCGTCTTTCAATGTGGGATGCAAGAAGTACAGCAGCTGATCACCAGCCT
GGATAATCGCCATTTCTACT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala Carboxypeptidase 3 Family; D-Alanyl-D-Alanine Carboxypeptidase; D-Alanyl-D-Alanine Carboxypeptidase/Endopeptidase; D-Ala-D-Ala Carboxypeptidase; Carboxypeptidase

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MICPPRLACIFIALSLIISGCSNSDIQPSSSPSPEPTSSVPEPPPPLQLGLADPETVANSQIETYLSQLGAPTVSQGVWI
QTQDTLLANHQGTIPLPAASITKVATSLAVLKKLGPDHRFETKIGYIGTLQNGELNGDLVIEGGADPFFVWEDAIALGNL
LNQNGIRNVSGNLIVVGPFYMNYEPDPVTSGTLLQQGLDHTRWPAEAQTQYQTLPADTAKPEVAIAGNIQTAASRPDQVK
PLIQHRSLPVAELLKKMNQYSNNAMSEMLAEYVGGATQVAQMAAQEAGVPSQEIQLINGSGLGVDNRISPRAACGMFQAI
AHLLHLQEMTIADIFAVIGTDPGVHNERPLPQKFVMKSGTLNRVSSIAGALPTKNQGVIWFAVINGEGNVDQFRAQQETL
LQNLMQSWGTTNKTFDLITANPTRQNLTTTSKIVLSN

Sequences:

>Translated_437_residues
MICPPRLACIFIALSLIISGCSNSDIQPSSSPSPEPTSSVPEPPPPLQLGLADPETVANSQIETYLSQLGAPTVSQGVWI
QTQDTLLANHQGTIPLPAASITKVATSLAVLKKLGPDHRFETKIGYIGTLQNGELNGDLVIEGGADPFFVWEDAIALGNL
LNQNGIRNVSGNLIVVGPFYMNYEPDPVTSGTLLQQGLDHTRWPAEAQTQYQTLPADTAKPEVAIAGNIQTAASRPDQVK
PLIQHRSLPVAELLKKMNQYSNNAMSEMLAEYVGGATQVAQMAAQEAGVPSQEIQLINGSGLGVDNRISPRAACGMFQAI
AHLLHLQEMTIADIFAVIGTDPGVHNERPLPQKFVMKSGTLNRVSSIAGALPTKNQGVIWFAVINGEGNVDQFRAQQETL
LQNLMQSWGTTNKTFDLITANPTRQNLTTTSKIVLSN
>Mature_437_residues
MICPPRLACIFIALSLIISGCSNSDIQPSSSPSPEPTSSVPEPPPPLQLGLADPETVANSQIETYLSQLGAPTVSQGVWI
QTQDTLLANHQGTIPLPAASITKVATSLAVLKKLGPDHRFETKIGYIGTLQNGELNGDLVIEGGADPFFVWEDAIALGNL
LNQNGIRNVSGNLIVVGPFYMNYEPDPVTSGTLLQQGLDHTRWPAEAQTQYQTLPADTAKPEVAIAGNIQTAASRPDQVK
PLIQHRSLPVAELLKKMNQYSNNAMSEMLAEYVGGATQVAQMAAQEAGVPSQEIQLINGSGLGVDNRISPRAACGMFQAI
AHLLHLQEMTIADIFAVIGTDPGVHNERPLPQKFVMKSGTLNRVSSIAGALPTKNQGVIWFAVINGEGNVDQFRAQQETL
LQNLMQSWGTTNKTFDLITANPTRQNLTTTSKIVLSN

Specific function: Not Involved In Transpeptidation But Exclusively Catalyzes A Dd-Carboxypeptidase And Dd-Endopeptidase Reaction. [C]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789573, Length=98, Percent_Identity=36.734693877551, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4; 3.4.99.- [C]

Molecular weight: Translated: 46796; Mature: 46796

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICPPRLACIFIALSLIISGCSNSDIQPSSSPSPEPTSSVPEPPPPLQLGLADPETVANS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHH
QIETYLSQLGAPTVSQGVWIQTQDTLLANHQGTIPLPAASITKVATSLAVLKKLGPDHRF
HHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
ETKIGYIGTLQNGELNGDLVIEGGADPFFVWEDAIALGNLLNQNGIRNVSGNLIVVGPFY
ECCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCEEEEECEE
MNYEPDPVTSGTLLQQGLDHTRWPAEAQTQYQTLPADTAKPEVAIAGNIQTAASRPDQVK
ECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCHHHHCCCHHHH
PLIQHRSLPVAELLKKMNQYSNNAMSEMLAEYVGGATQVAQMAAQEAGVPSQEIQLINGS
HHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHEEEECCC
GLGVDNRISPRAACGMFQAIAHLLHLQEMTIADIFAVIGTDPGVHNERPLPQKFVMKSGT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCC
LNRVSSIAGALPTKNQGVIWFAVINGEGNVDQFRAQQETLLQNLMQSWGTTNKTFDLITA
HHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
NPTRQNLTTTSKIVLSN
CCCCCCCCCCCEEEECC
>Mature Secondary Structure
MICPPRLACIFIALSLIISGCSNSDIQPSSSPSPEPTSSVPEPPPPLQLGLADPETVANS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHH
QIETYLSQLGAPTVSQGVWIQTQDTLLANHQGTIPLPAASITKVATSLAVLKKLGPDHRF
HHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
ETKIGYIGTLQNGELNGDLVIEGGADPFFVWEDAIALGNLLNQNGIRNVSGNLIVVGPFY
ECCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCEEEEECEE
MNYEPDPVTSGTLLQQGLDHTRWPAEAQTQYQTLPADTAKPEVAIAGNIQTAASRPDQVK
ECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCHHHHCCCHHHH
PLIQHRSLPVAELLKKMNQYSNNAMSEMLAEYVGGATQVAQMAAQEAGVPSQEIQLINGS
HHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHEEEECCC
GLGVDNRISPRAACGMFQAIAHLLHLQEMTIADIFAVIGTDPGVHNERPLPQKFVMKSGT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCC
LNRVSSIAGALPTKNQGVIWFAVINGEGNVDQFRAQQETLLQNLMQSWGTTNKTFDLITA
HHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
NPTRQNLTTTSKIVLSN
CCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Preferential cleavage is (Ac)2-L-Lys-D-Ala D-Ala and Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine [C]

General reaction: Hydrolase; Acting on peptide bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA