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The map label for this gene is ddl

Identifier: 158336262

GI number: 158336262

Start: 3154942

End: 3156000

Strand: Direct

Name: ddl

Synonym: AM1_3124

Alternate gene names: 158336262

Gene position: 3154942-3156000 (Clockwise)

Preceding gene: 158336260

Following gene: 158336263

Centisome position: 48.51

GC content: 49.76

Gene sequence:

>1059_bases
GTGGAGAAATTGCGAGTCGGTTTAGTGTTTGGGGGATGTTCGGGAGAGCATGAAGTATCCATTACCTCAGCGAAAGCCGT
TTGCGGTGCACTTCAAACTGCGCCTAATCCCGATAAGTATGAGGTGATTCCCTTTTACATCCATAAAAATGGGTGTTGGC
AAGCGGGGGAAGTGGCGAATCAAGTTTTAGAAATGGGGAAACCTCAAGATCAAGCGGAAGAAGGCAGTCGATGGCATTTT
CCTGAGGCGGCAGCTTCTATCGATGTCTGGTTTCCGATCTTGCATGGCCCCAATGGGGAAGATGGCACGATTCAGGGGCT
ATTGCAATTGATGCAAGTGCCTTATGTGGGTTCTGGCGTGTTGGGTTCGGCAGCGGGCATGGATAAGATTGCCATGAAAA
CCGTGTTTGCAGCGGCGGGATTGCCTCAGGTGAAGTATGAGTCTGTGACTCGGGAGCAGGTCTGGTCTGATCCTTGTGTG
TTTAAGCAAGTGTGCGATCGCATCGATGAAACCATTGGCTATCCCAACTTCGTTAAGCCCGCCAACCTCGGATCGTCCGT
GGGCATTTCTAAGGTTCGATCTCGGCTGGAACTAGAAGCGGCTCTAGATAGTGCTGCTTCCTTTGATCGCCGCATTGTTG
TAGAAGCAGGCGTAGTGGCTCGGGAAGTAGAATGTGCGGTTTTAGGGAATGGCCGACCTCGGGCATCGGTAGTGGGAGAA
ATTAGCTTTGATAGTGATTTCTACGACTACGAAACGAAATATACGGAAGGACGAGCGAGTCTGCAAATTCCAGCCCCATT
GCCCGCGGATATCACCGAAAAAATTCAAGAGATGGCAATCAACGCTTTTATTGCAGTCGATGCGGCGGGCCTATCGCGGG
TAGATTTCTTTTATGTGGAATCTACTGGGGAGGTGTTGATCAATGAAATCAATACCTTGCCTGGGTTTACATCAACCAGC
ATGTATCCCATGCTCTGGAGCGTTTCAGGCGTCGATTTTCCCAAATTAGTGGATCGACTGATTCAATTGGCTGTGGAATA
TCATGCTCCTGCCGATTAG

Upstream 100 bases:

>100_bases
AAGGTAGAGTTGGAAGGTCTCTGATCACTAAGGTCTTTATCCTAGATTTATAAGTGGTTGTGATAGTCTATCTGGATTGA
CGTGTGCCTGGGAGTTAACT

Downstream 100 bases:

>100_bases
CCCGGTCTGTAAGAAAAACGGTTTTTGGGTGGTGGATGATGATTTGGGATTGATCCGTCAACTGCTTGCTGTCGATTAAT
TAGATTTTTTTGAAAAGCAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MEKLRVGLVFGGCSGEHEVSITSAKAVCGALQTAPNPDKYEVIPFYIHKNGCWQAGEVANQVLEMGKPQDQAEEGSRWHF
PEAAASIDVWFPILHGPNGEDGTIQGLLQLMQVPYVGSGVLGSAAGMDKIAMKTVFAAAGLPQVKYESVTREQVWSDPCV
FKQVCDRIDETIGYPNFVKPANLGSSVGISKVRSRLELEAALDSAASFDRRIVVEAGVVAREVECAVLGNGRPRASVVGE
ISFDSDFYDYETKYTEGRASLQIPAPLPADITEKIQEMAINAFIAVDAAGLSRVDFFYVESTGEVLINEINTLPGFTSTS
MYPMLWSVSGVDFPKLVDRLIQLAVEYHAPAD

Sequences:

>Translated_352_residues
MEKLRVGLVFGGCSGEHEVSITSAKAVCGALQTAPNPDKYEVIPFYIHKNGCWQAGEVANQVLEMGKPQDQAEEGSRWHF
PEAAASIDVWFPILHGPNGEDGTIQGLLQLMQVPYVGSGVLGSAAGMDKIAMKTVFAAAGLPQVKYESVTREQVWSDPCV
FKQVCDRIDETIGYPNFVKPANLGSSVGISKVRSRLELEAALDSAASFDRRIVVEAGVVAREVECAVLGNGRPRASVVGE
ISFDSDFYDYETKYTEGRASLQIPAPLPADITEKIQEMAINAFIAVDAAGLSRVDFFYVESTGEVLINEINTLPGFTSTS
MYPMLWSVSGVDFPKLVDRLIQLAVEYHAPAD
>Mature_352_residues
MEKLRVGLVFGGCSGEHEVSITSAKAVCGALQTAPNPDKYEVIPFYIHKNGCWQAGEVANQVLEMGKPQDQAEEGSRWHF
PEAAASIDVWFPILHGPNGEDGTIQGLLQLMQVPYVGSGVLGSAAGMDKIAMKTVFAAAGLPQVKYESVTREQVWSDPCV
FKQVCDRIDETIGYPNFVKPANLGSSVGISKVRSRLELEAALDSAASFDRRIVVEAGVVAREVECAVLGNGRPRASVVGE
ISFDSDFYDYETKYTEGRASLQIPAPLPADITEKIQEMAINAFIAVDAAGLSRVDFFYVESTGEVLINEINTLPGFTSTS
MYPMLWSVSGVDFPKLVDRLIQLAVEYHAPAD

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=366, Percent_Identity=43.1693989071038, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1786280, Length=343, Percent_Identity=33.8192419825073, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ACAM1 (B0CEL5)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517436.1
- ProteinModelPortal:   B0CEL5
- SMR:   B0CEL5
- GeneID:   5681932
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3124
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   AMAR329726:AM1_3124-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38147; Mature: 38147

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLRVGLVFGGCSGEHEVSITSAKAVCGALQTAPNPDKYEVIPFYIHKNGCWQAGEVAN
CCCEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHH
QVLEMGKPQDQAEEGSRWHFPEAAASIDVWFPILHGPNGEDGTIQGLLQLMQVPYVGSGV
HHHHCCCCCCHHHCCCCCCCCHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
LGSAAGMDKIAMKTVFAAAGLPQVKYESVTREQVWSDPCVFKQVCDRIDETIGYPNFVKP
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
ANLGSSVGISKVRSRLELEAALDSAASFDRRIVVEAGVVAREVECAVLGNGRPRASVVGE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEECCCCCCCEEEEE
ISFDSDFYDYETKYTEGRASLQIPAPLPADITEKIQEMAINAFIAVDAAGLSRVDFFYVE
EECCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEE
STGEVLINEINTLPGFTSTSMYPMLWSVSGVDFPKLVDRLIQLAVEYHAPAD
CCCHHHHHHHHCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKLRVGLVFGGCSGEHEVSITSAKAVCGALQTAPNPDKYEVIPFYIHKNGCWQAGEVAN
CCCEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHH
QVLEMGKPQDQAEEGSRWHFPEAAASIDVWFPILHGPNGEDGTIQGLLQLMQVPYVGSGV
HHHHCCCCCCHHHCCCCCCCCHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
LGSAAGMDKIAMKTVFAAAGLPQVKYESVTREQVWSDPCVFKQVCDRIDETIGYPNFVKP
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
ANLGSSVGISKVRSRLELEAALDSAASFDRRIVVEAGVVAREVECAVLGNGRPRASVVGE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEECCCCCCCEEEEE
ISFDSDFYDYETKYTEGRASLQIPAPLPADITEKIQEMAINAFIAVDAAGLSRVDFFYVE
EECCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEE
STGEVLINEINTLPGFTSTSMYPMLWSVSGVDFPKLVDRLIQLAVEYHAPAD
CCCHHHHHHHHCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA