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The map label for this gene is 158336240

Identifier: 158336240

GI number: 158336240

Start: 3132095

End: 3133546

Strand: Reverse

Name: 158336240

Synonym: AM1_3102

Alternate gene names: NA

Gene position: 3133546-3132095 (Counterclockwise)

Preceding gene: 158336241

Following gene: 158336239

Centisome position: 48.18

GC content: 50.83

Gene sequence:

>1452_bases
ATGACCGTTTCTGCTCCAACCCGTAACACCTACCTCGACCATAATTGGCAGGGAGGCCACCAATCTCTGAAAGAGGAATA
CGACTATGCGATCGCAGACATAGACGGTGAAATTCCCGCTGAGCTGAGCGGCACTCTATTTCGCAACGGTCCGGGGCTAT
TGGATGTCAACGGTACTCCACTCCAGCATCCTTTTGATGGAGACGGTATGATTTGTTCGATTCAGTTCGATCAGGGCAAG
GCCCATTTCCGTAACCGCTTTGTCCGTACAGAAGGCTATTTAGCAGAACAAGAAGCGGGCAAGATTCTGTACCGTGGCGT
ATTTGGTACCCCTAAACCCGGCGGTTTACTGGCCAACGCCTTCGATATCAACTTTAAGAATATTGCCAACACTCATATCA
TCTACTGGGCAGGCAAGCTACTGGCCCTTTGGGAAGCCGATCAGCCCTATCGTCTCGATCCATCCACCTTAGACACATTG
GGCTTGGAAGACTTTTCGGGTCTTCTCAACCCTGGTGATCCATTTTCTGCTCACCCCCGAATCTTGCCCCATGCGGACGG
CGATCGCCTCGTTAACTTTGCCATCAAACCTGGCCTTTCCAGCAAGGTAAAACTGTTCGAGTTTGACCCATCCGGTGAGG
TGGTTCACCAGCAGCAGTTTGTCATTCCAGGGTTTGCCTTTATTCACGATTTTGCCGTTACCCCCAACTACTACATCTTT
TTCCAAAATCCCGTCATTCTGAATCCCATTCCCTTCCTATTGGGGTTCAAAGGGGCAGGGGAATGTATCTCGTTTAAGCC
TGGTAGTCCCACCAAAATTTGGCTGGTCCGACGAGGCGAGAATAAACCCCAGCAGCTAGACACGGATGCTTGCTTTGTCT
TCCACCATGCCAATGCCTATGAAGAAGATGGTCAAGTCATTGTAGACTCCATCTGCTACGACACTTTCCCAGGCTTAGAA
TCGGGGATTGATTTCCGCACCATTGATTTTGACGGGGTTCCTCCCGGCTCTTTATGGCGATTCCATCTCAATCCTGAGAC
CGCCACCGTTGAGAAAACACCGCTAATCCAGCGCAGTTGTGAGTTTCCAACCCTGAATCCTGCAAATGTGGCCCACGATT
ACCGCTACGTCTATATTGGTGCCACGGATACCAGTACGGGCAACGCGCCTTTACAAGCCATCCTGAAGCAAGACGTTAAA
ACAGGCGAAGAACAAGTCTGGAGTGCCGCCCCTCAAGGCTTTATGGGAGAACCGGTGTTTATCCCCAAACCTAACGCCAC
CCAAGAAGATCACGGTTGGTTAATCGCGCTGGTTTACGATGGCGATCACGATCGATCCGATGTGGTTATCCTTGATGCCG
AGCGAATCGAACAGGGACCGCTGGCCCGCTTGCACTTAAAGCACCATATCCCCTACGGTTTACATGGCAGTTTTACGCCA
ACGCTGTTTTAA

Upstream 100 bases:

>100_bases
AGATCAAGACCTTGCTTTGAATATCAATCCACCTGTCGAGCCGCTTTTAGCTGAGTCTCTCGCTGTTGAACTTGTTACTG
AACCTCGTAATTGAATCCCT

Downstream 100 bases:

>100_bases
CCGGTCCCAACCCGATGCCCCAAAAGCTTTTGTTTGTCTGTCTAGGCAATATTTGCCGCTCCCCCTCTGCCGAAGGGATT
ATGAATCACCTGCTGCAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTVSAPTRNTYLDHNWQGGHQSLKEEYDYAIADIDGEIPAELSGTLFRNGPGLLDVNGTPLQHPFDGDGMICSIQFDQGK
AHFRNRFVRTEGYLAEQEAGKILYRGVFGTPKPGGLLANAFDINFKNIANTHIIYWAGKLLALWEADQPYRLDPSTLDTL
GLEDFSGLLNPGDPFSAHPRILPHADGDRLVNFAIKPGLSSKVKLFEFDPSGEVVHQQQFVIPGFAFIHDFAVTPNYYIF
FQNPVILNPIPFLLGFKGAGECISFKPGSPTKIWLVRRGENKPQQLDTDACFVFHHANAYEEDGQVIVDSICYDTFPGLE
SGIDFRTIDFDGVPPGSLWRFHLNPETATVEKTPLIQRSCEFPTLNPANVAHDYRYVYIGATDTSTGNAPLQAILKQDVK
TGEEQVWSAAPQGFMGEPVFIPKPNATQEDHGWLIALVYDGDHDRSDVVILDAERIEQGPLARLHLKHHIPYGLHGSFTP
TLF

Sequences:

>Translated_483_residues
MTVSAPTRNTYLDHNWQGGHQSLKEEYDYAIADIDGEIPAELSGTLFRNGPGLLDVNGTPLQHPFDGDGMICSIQFDQGK
AHFRNRFVRTEGYLAEQEAGKILYRGVFGTPKPGGLLANAFDINFKNIANTHIIYWAGKLLALWEADQPYRLDPSTLDTL
GLEDFSGLLNPGDPFSAHPRILPHADGDRLVNFAIKPGLSSKVKLFEFDPSGEVVHQQQFVIPGFAFIHDFAVTPNYYIF
FQNPVILNPIPFLLGFKGAGECISFKPGSPTKIWLVRRGENKPQQLDTDACFVFHHANAYEEDGQVIVDSICYDTFPGLE
SGIDFRTIDFDGVPPGSLWRFHLNPETATVEKTPLIQRSCEFPTLNPANVAHDYRYVYIGATDTSTGNAPLQAILKQDVK
TGEEQVWSAAPQGFMGEPVFIPKPNATQEDHGWLIALVYDGDHDRSDVVILDAERIEQGPLARLHLKHHIPYGLHGSFTP
TLF
>Mature_482_residues
TVSAPTRNTYLDHNWQGGHQSLKEEYDYAIADIDGEIPAELSGTLFRNGPGLLDVNGTPLQHPFDGDGMICSIQFDQGKA
HFRNRFVRTEGYLAEQEAGKILYRGVFGTPKPGGLLANAFDINFKNIANTHIIYWAGKLLALWEADQPYRLDPSTLDTLG
LEDFSGLLNPGDPFSAHPRILPHADGDRLVNFAIKPGLSSKVKLFEFDPSGEVVHQQQFVIPGFAFIHDFAVTPNYYIFF
QNPVILNPIPFLLGFKGAGECISFKPGSPTKIWLVRRGENKPQQLDTDACFVFHHANAYEEDGQVIVDSICYDTFPGLES
GIDFRTIDFDGVPPGSLWRFHLNPETATVEKTPLIQRSCEFPTLNPANVAHDYRYVYIGATDTSTGNAPLQAILKQDVKT
GEEQVWSAAPQGFMGEPVFIPKPNATQEDHGWLIALVYDGDHDRSDVVILDAERIEQGPLARLHLKHHIPYGLHGSFTPT
LF

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI74027270, Length=523, Percent_Identity=27.151051625239, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI4506591, Length=536, Percent_Identity=24.4402985074627, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI82617624, Length=523, Percent_Identity=24.282982791587, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI82617628, Length=523, Percent_Identity=24.282982791587, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71998242, Length=514, Percent_Identity=26.2645914396887, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32564756, Length=513, Percent_Identity=25.9259259259259, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24646669, Length=359, Percent_Identity=24.2339832869081, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 53719; Mature: 53588

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSAPTRNTYLDHNWQGGHQSLKEEYDYAIADIDGEIPAELSGTLFRNGPGLLDVNGTP
CCCCCCCCCCEECCCCCCHHHHHHHHHCEEEEECCCCCCCCCCCEEEECCCCEEECCCCC
LQHPFDGDGMICSIQFDQGKAHFRNRFVRTEGYLAEQEAGKILYRGVFGTPKPGGLLANA
CCCCCCCCCEEEEEEECCCHHHHHHHEEECCCCEEHHCCCEEEEEECCCCCCCCCEEEEE
FDINFKNIANTHIIYWAGKLLALWEADQPYRLDPSTLDTLGLEDFSGLLNPGDPFSAHPR
EECCHHHCCCCEEEEEECEEEEEEECCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCE
ILPHADGDRLVNFAIKPGLSSKVKLFEFDPSGEVVHQQQFVIPGFAFIHDFAVTPNYYIF
EECCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEECEEEECCCCHHEEEEECCCCEEEE
FQNPVILNPIPFLLGFKGAGECISFKPGSPTKIWLVRRGENKPQQLDTDACFVFHHANAY
ECCCEEECCCHHEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCC
EEDGQVIVDSICYDTFPGLESGIDFRTIDFDGVPPGSLWRFHLNPETATVEKTPLIQRSC
CCCCCEEEEEHHHCCCCCHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEECCCCHHHCC
EFPTLNPANVAHDYRYVYIGATDTSTGNAPLQAILKQDVKTGEEQVWSAAPQGFMGEPVF
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEE
IPKPNATQEDHGWLIALVYDGDHDRSDVVILDAERIEQGPLARLHLKHHIPYGLHGSFTP
EECCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCCEEEEEEEECCCCCCCCCCCC
TLF
CCC
>Mature Secondary Structure 
TVSAPTRNTYLDHNWQGGHQSLKEEYDYAIADIDGEIPAELSGTLFRNGPGLLDVNGTP
CCCCCCCCCEECCCCCCHHHHHHHHHCEEEEECCCCCCCCCCCEEEECCCCEEECCCCC
LQHPFDGDGMICSIQFDQGKAHFRNRFVRTEGYLAEQEAGKILYRGVFGTPKPGGLLANA
CCCCCCCCCEEEEEEECCCHHHHHHHEEECCCCEEHHCCCEEEEEECCCCCCCCCEEEEE
FDINFKNIANTHIIYWAGKLLALWEADQPYRLDPSTLDTLGLEDFSGLLNPGDPFSAHPR
EECCHHHCCCCEEEEEECEEEEEEECCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCE
ILPHADGDRLVNFAIKPGLSSKVKLFEFDPSGEVVHQQQFVIPGFAFIHDFAVTPNYYIF
EECCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEECEEEECCCCHHEEEEECCCCEEEE
FQNPVILNPIPFLLGFKGAGECISFKPGSPTKIWLVRRGENKPQQLDTDACFVFHHANAY
ECCCEEECCCHHEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCC
EEDGQVIVDSICYDTFPGLESGIDFRTIDFDGVPPGSLWRFHLNPETATVEKTPLIQRSC
CCCCCEEEEEHHHCCCCCHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEECCCCHHHCC
EFPTLNPANVAHDYRYVYIGATDTSTGNAPLQAILKQDVKTGEEQVWSAAPQGFMGEPVF
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEE
IPKPNATQEDHGWLIALVYDGDHDRSDVVILDAERIEQGPLARLHLKHHIPYGLHGSFTP
EECCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCCEEEEEEEECCCCCCCCCCCC
TLF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]