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The map label for this gene is ilvB [H]

Identifier: 158336223

GI number: 158336223

Start: 3115013

End: 3116821

Strand: Reverse

Name: ilvB [H]

Synonym: AM1_3085

Alternate gene names: 158336223

Gene position: 3116821-3115013 (Counterclockwise)

Preceding gene: 158336224

Following gene: 158336222

Centisome position: 47.92

GC content: 51.63

Gene sequence:

>1809_bases
GTGCGTGCAACCGGAGCTTTTGCGTTAATCGATAGTCTAAAAAATCACGGCGTCAAACATATTTTTGGCTATCCCGGTGG
TGCGATTTTGCCGATTTATGATGAGGTGTATAAGGCAGAACAGCGGGGCGATCTCAAACATATTTTGGTCCGCCATGAAC
AAGGTGCATCCCATGCTGCTGATGGCTATGCTAGAGCAACGGGGCAGGTCGGCATCTGCTTTGGGACCTCCGGTCCTGGG
GCAACCAACTTGGTGACCGGAATTGCCACCGCTCATATGGATTCAATTCCGATGGTGGTGATTACGGGGCAGGTGCCTCG
GACCAATATTGGCAGTGATGCGTTCCAAGAAACCGATATTTTTGGGATTACGCTGCCCCTGGTGAAACATTCCTATGTGG
TGCGTGATCCGGCGGATATGGCTCGGGTTGTGGCGGAAGCCTTCCATATTGCTGGTACCGGTAGACCAGGACCCGTTTTA
ATTGATGTGCCCAAGGACGTAGGCCTTCAAGAGTTTGACTATACTCCTGTGCAGCCAGGGACCATTAGCTTAAAGGGCTA
TCGCCCCACGGTCAAAGGCAATCCGCGTCAGATTAAACAAGCCCTAGATCTCATTCGACAGGCTAAATCTCCCCTCTTAT
ATGTAGGTGGTGGAGCCATTTCGTCGGGAGCCCATGCTGAACTGAAAGAGCTGGCTGAGTGGTTCCATATTCCGGTAACC
ACAACGCTGATGGGCAAAGGCTCCTTTGATGAGGCCCATCCCTTATCCGTGGGGATGTTGGGTATGCACGGCACCGCCTA
TGCCAATTTTGCCGTTAGTGAATGTGATCTGCTGATTGCCGTGGGTGCCCGCTTTGATGATCGGGTGACGGGTAAGCTTG
ATGAATTTGCCTCTAGGGCAAAAGTGATTCATATCGATATTGACCCAGCGGAAGTGGGCAAAAATCGTGCCCCGACGGTG
CCGATTGTGGGTAGCGTGAAGCCTGTCTTAGTGGAACTGCTGAAAATAGCAAAACAGTCTTCACCGCCTGATACAGCCCA
GACACAAGAGTGGCGCTCTCGGGTAGAACGTTGGCAGCGAGATTATCCATTGATGGTGCCTAGACCAGACACAAAGCTGT
CGCCCCAAGAGGTGATTGTGGAGCTGCAACGACAGGCACCCCATGCCTACTACACAACAGATGTCGGACAGCACCAAATG
TGGGCGGCTCAGTTCCTGAAGAATGGTCCTCGCCAGTGGGTGACGAGTGGTGGTCTGGGAACCATGGGCTATGGTTTACC
GGCGGCGATGGGGGTACAGGTGGCATTACCCGATCATCAAGTGATCTGTATCAGTGGTGATGCCAGCTTCCAAATGAATT
TGCAGGAGTTGGCTACCCTAGCCCAATACGGTATCAAGGTCAAAACGGTCATTATCAATAATTTCTGGCAAGGGATGGTG
CGCCAGTGGCAGCAAGCCTTTTTTGGCGAGCGCTATTCCAACTCCAGTATGGAAGCCGGGATGCCTAATTTTGAGAAATT
GGCGGAAGCATTTGGCGTCAAGGGTGGCATTTTGCGGGAGCGAAATGATCTCGAAAGTGTCATTGCTGAGATGTTAGCGT
TTGATGGTCCCTATCTGTTAGATGCCCAGGTGACGCGGGATGAAAACTGCTATCCCATGGTGGCACCGGGTAAGAGCAAT
GCCCAAATGCTAGGGTTGCCGGATCAGAAAACCTTAGAAAAGGCAGCAGAGCTGATTTATTGCAGTAACTGCGGGGCCAA
AAATACCTCAACAAATCACTTTTGCCCTGAGTGCGGGACAAAGTTGTAA

Upstream 100 bases:

>100_bases
GTCGCTCCGCTTAAACTATATAGATCTAAAAGGTTTTCATTTCTCAACGGACGATAAATTCTCTTATTTGGCAACTAGGA
TCTGAGTGTGAGGTTCTCCT

Downstream 100 bases:

>100_bases
GCATGAATTTGCAGCCGCAGGTGCAAGCACTGATTGATGAAGCGCCGCAAGACGGTGGGATGCCTGCTGCGATTCGAATT
ATTGCGCCGTTGCTGCAAGA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MRATGAFALIDSLKNHGVKHIFGYPGGAILPIYDEVYKAEQRGDLKHILVRHEQGASHAADGYARATGQVGICFGTSGPG
ATNLVTGIATAHMDSIPMVVITGQVPRTNIGSDAFQETDIFGITLPLVKHSYVVRDPADMARVVAEAFHIAGTGRPGPVL
IDVPKDVGLQEFDYTPVQPGTISLKGYRPTVKGNPRQIKQALDLIRQAKSPLLYVGGGAISSGAHAELKELAEWFHIPVT
TTLMGKGSFDEAHPLSVGMLGMHGTAYANFAVSECDLLIAVGARFDDRVTGKLDEFASRAKVIHIDIDPAEVGKNRAPTV
PIVGSVKPVLVELLKIAKQSSPPDTAQTQEWRSRVERWQRDYPLMVPRPDTKLSPQEVIVELQRQAPHAYYTTDVGQHQM
WAAQFLKNGPRQWVTSGGLGTMGYGLPAAMGVQVALPDHQVICISGDASFQMNLQELATLAQYGIKVKTVIINNFWQGMV
RQWQQAFFGERYSNSSMEAGMPNFEKLAEAFGVKGGILRERNDLESVIAEMLAFDGPYLLDAQVTRDENCYPMVAPGKSN
AQMLGLPDQKTLEKAAELIYCSNCGAKNTSTNHFCPECGTKL

Sequences:

>Translated_602_residues
MRATGAFALIDSLKNHGVKHIFGYPGGAILPIYDEVYKAEQRGDLKHILVRHEQGASHAADGYARATGQVGICFGTSGPG
ATNLVTGIATAHMDSIPMVVITGQVPRTNIGSDAFQETDIFGITLPLVKHSYVVRDPADMARVVAEAFHIAGTGRPGPVL
IDVPKDVGLQEFDYTPVQPGTISLKGYRPTVKGNPRQIKQALDLIRQAKSPLLYVGGGAISSGAHAELKELAEWFHIPVT
TTLMGKGSFDEAHPLSVGMLGMHGTAYANFAVSECDLLIAVGARFDDRVTGKLDEFASRAKVIHIDIDPAEVGKNRAPTV
PIVGSVKPVLVELLKIAKQSSPPDTAQTQEWRSRVERWQRDYPLMVPRPDTKLSPQEVIVELQRQAPHAYYTTDVGQHQM
WAAQFLKNGPRQWVTSGGLGTMGYGLPAAMGVQVALPDHQVICISGDASFQMNLQELATLAQYGIKVKTVIINNFWQGMV
RQWQQAFFGERYSNSSMEAGMPNFEKLAEAFGVKGGILRERNDLESVIAEMLAFDGPYLLDAQVTRDENCYPMVAPGKSN
AQMLGLPDQKTLEKAAELIYCSNCGAKNTSTNHFCPECGTKL
>Mature_602_residues
MRATGAFALIDSLKNHGVKHIFGYPGGAILPIYDEVYKAEQRGDLKHILVRHEQGASHAADGYARATGQVGICFGTSGPG
ATNLVTGIATAHMDSIPMVVITGQVPRTNIGSDAFQETDIFGITLPLVKHSYVVRDPADMARVVAEAFHIAGTGRPGPVL
IDVPKDVGLQEFDYTPVQPGTISLKGYRPTVKGNPRQIKQALDLIRQAKSPLLYVGGGAISSGAHAELKELAEWFHIPVT
TTLMGKGSFDEAHPLSVGMLGMHGTAYANFAVSECDLLIAVGARFDDRVTGKLDEFASRAKVIHIDIDPAEVGKNRAPTV
PIVGSVKPVLVELLKIAKQSSPPDTAQTQEWRSRVERWQRDYPLMVPRPDTKLSPQEVIVELQRQAPHAYYTTDVGQHQM
WAAQFLKNGPRQWVTSGGLGTMGYGLPAAMGVQVALPDHQVICISGDASFQMNLQELATLAQYGIKVKTVIINNFWQGMV
RQWQQAFFGERYSNSSMEAGMPNFEKLAEAFGVKGGILRERNDLESVIAEMLAFDGPYLLDAQVTRDENCYPMVAPGKSN
AQMLGLPDQKTLEKAAELIYCSNCGAKNTSTNHFCPECGTKL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=553, Percent_Identity=26.7631103074141, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=25.2252252252252, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=46.572934973638, Blast_Score=510, Evalue=1e-145,
Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=44.7971781305115, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=568, Percent_Identity=34.6830985915493, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1787096, Length=564, Percent_Identity=28.0141843971631, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788716, Length=481, Percent_Identity=27.2349272349272, Blast_Score=165, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=552, Percent_Identity=26.9927536231884, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=576, Percent_Identity=27.0833333333333, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=576, Percent_Identity=27.0833333333333, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=43.8687392055268, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=493, Percent_Identity=27.1805273833671, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=501, Percent_Identity=24.9500998003992, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=502, Percent_Identity=24.3027888446215, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19922626, Length=566, Percent_Identity=25.7950530035336, Blast_Score=169, Evalue=6e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65410; Mature: 65410

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATGAFALIDSLKNHGVKHIFGYPGGAILPIYDEVYKAEQRGDLKHILVRHEQGASHAA
CCCCHHHHHHHHHHHCCCEEEECCCCCCEEECHHHHHHHHHCCCHHHHHEECCCCCCCCC
DGYARATGQVGICFGTSGPGATNLVTGIATAHMDSIPMVVITGQVPRTNIGSDAFQETDI
CCHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCCE
FGITLPLVKHSYVVRDPADMARVVAEAFHIAGTGRPGPVLIDVPKDVGLQEFDYTPVQPG
EEEEHHHHHCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
TISLKGYRPTVKGNPRQIKQALDLIRQAKSPLLYVGGGAISSGAHAELKELAEWFHIPVT
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEE
TTLMGKGSFDEAHPLSVGMLGMHGTAYANFAVSECDLLIAVGARFDDRVTGKLDEFASRA
EEEECCCCCCCCCCCEEEEEECCCCHHCCEEHHCCCEEEEECCCCCCCCCCCHHHHHCCC
KVIHIDIDPAEVGKNRAPTVPIVGSVKPVLVELLKIAKQSSPPDTAQTQEWRSRVERWQR
EEEEEECCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
DYPLMVPRPDTKLSPQEVIVELQRQAPHAYYTTDVGQHQMWAAQFLKNGPRQWVTSGGLG
CCCEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCC
TMGYGLPAAMGVQVALPDHQVICISGDASFQMNLQELATLAQYGIKVKTVIINNFWQGMV
CCCCCCHHHCCEEEECCCCEEEEEECCCCEEECHHHHHHHHHCCCEEEEEEHHHHHHHHH
RQWQQAFFGERYSNSSMEAGMPNFEKLAEAFGVKGGILRERNDLESVIAEMLAFDGPYLL
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
DAQVTRDENCYPMVAPGKSNAQMLGLPDQKTLEKAAELIYCSNCGAKNTSTNHFCPECGT
EEEECCCCCCCEEECCCCCCCEEECCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCC
KL
CC
>Mature Secondary Structure
MRATGAFALIDSLKNHGVKHIFGYPGGAILPIYDEVYKAEQRGDLKHILVRHEQGASHAA
CCCCHHHHHHHHHHHCCCEEEECCCCCCEEECHHHHHHHHHCCCHHHHHEECCCCCCCCC
DGYARATGQVGICFGTSGPGATNLVTGIATAHMDSIPMVVITGQVPRTNIGSDAFQETDI
CCHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCCE
FGITLPLVKHSYVVRDPADMARVVAEAFHIAGTGRPGPVLIDVPKDVGLQEFDYTPVQPG
EEEEHHHHHCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
TISLKGYRPTVKGNPRQIKQALDLIRQAKSPLLYVGGGAISSGAHAELKELAEWFHIPVT
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEE
TTLMGKGSFDEAHPLSVGMLGMHGTAYANFAVSECDLLIAVGARFDDRVTGKLDEFASRA
EEEECCCCCCCCCCCEEEEEECCCCHHCCEEHHCCCEEEEECCCCCCCCCCCHHHHHCCC
KVIHIDIDPAEVGKNRAPTVPIVGSVKPVLVELLKIAKQSSPPDTAQTQEWRSRVERWQR
EEEEEECCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
DYPLMVPRPDTKLSPQEVIVELQRQAPHAYYTTDVGQHQMWAAQFLKNGPRQWVTSGGLG
CCCEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCC
TMGYGLPAAMGVQVALPDHQVICISGDASFQMNLQELATLAQYGIKVKTVIINNFWQGMV
CCCCCCHHHCCEEEECCCCEEEEEECCCCEEECHHHHHHHHHCCCEEEEEEHHHHHHHHH
RQWQQAFFGERYSNSSMEAGMPNFEKLAEAFGVKGGILRERNDLESVIAEMLAFDGPYLL
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
DAQVTRDENCYPMVAPGKSNAQMLGLPDQKTLEKAAELIYCSNCGAKNTSTNHFCPECGT
EEEECCCCCCCEEECCCCCCCEEECCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCC
KL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1512571 [H]