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The map label for this gene is nfnB [C]

Identifier: 158336128

GI number: 158336128

Start: 3020622

End: 3021284

Strand: Direct

Name: nfnB [C]

Synonym: AM1_2990

Alternate gene names: 158336128

Gene position: 3020622-3021284 (Clockwise)

Preceding gene: 158336127

Following gene: 158336129

Centisome position: 46.44

GC content: 47.81

Gene sequence:

>663_bases
ATGAGTGATTCCACCCTGACCCCTGAACAACTTCTGCACCAACTCCAATGGCGCTATGCTACCAAAAAATTTGACCCCAC
CCAAAAAATACCCACAGCCACTTGGGAGGCCCTGGAACAAAGTTTAGTGCTCACCCCGTCCTCTTTTGGACTCCAACCCT
GGAAATTTTTTGTCGTTCAAAACTCACAAATTCGCCAGCAACTCCTGGAATATTCTTTCGGCCAAACTCAAGTTGTGGAC
GCCTCTCATCTGGTCGTCTTAGCCATTAAAAAAGCAGCCAATGCAGACGATGTTGATCGCTATATGCAGCGAACCGCAGA
AGTCCGTCAAGTCGACATCGACTCTTTAACGGGCTTTGCCAATGTCATTAAAAACTTTTTAGCCAACCCCCATAACCCTG
ACTTTGATCCAGATGAATGGGCAACCCGGCAGGTCTATATTGCCCTAGGCCAACTCATGACTAGTGCTGCAGTCCTCGGC
ATTGATGCTTGCCCTATGGAAGGCATTATCCCTGCCAAATATGATGAAGTCTTAGGCCTGACAGACACTGAATATCAAGC
CGTCCTAGCCTGTCCTGTCGGGTATCGTCATCCCGAAGATAAATATGCTGTGCTCCCTAAAATCAGGTATGCGCAAGAAG
AGATCGTGGCTCATATTTCCTGA

Upstream 100 bases:

>100_bases
ACGGGCTGCTCTCTGCTTTTCATAGGTGATCCGCTAACTTTTCTCAGCGTCCAAATATGTGATGCTTTTACCATCTACCC
TCTTCTAGCCTATTTCTGTT

Downstream 100 bases:

>100_bases
GGTATGAACCGCTCTAGAGCAGAGTCCTTACAGATATCTCATCATTCCAGTCTGTTCAACCCCCAGAGCTGTCTAGACTA
AGATGAGAAAAACATCTATG

Product: nitroreductase family protein

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSDSTLTPEQLLHQLQWRYATKKFDPTQKIPTATWEALEQSLVLTPSSFGLQPWKFFVVQNSQIRQQLLEYSFGQTQVVD
ASHLVVLAIKKAANADDVDRYMQRTAEVRQVDIDSLTGFANVIKNFLANPHNPDFDPDEWATRQVYIALGQLMTSAAVLG
IDACPMEGIIPAKYDEVLGLTDTEYQAVLACPVGYRHPEDKYAVLPKIRYAQEEIVAHIS

Sequences:

>Translated_220_residues
MSDSTLTPEQLLHQLQWRYATKKFDPTQKIPTATWEALEQSLVLTPSSFGLQPWKFFVVQNSQIRQQLLEYSFGQTQVVD
ASHLVVLAIKKAANADDVDRYMQRTAEVRQVDIDSLTGFANVIKNFLANPHNPDFDPDEWATRQVYIALGQLMTSAAVLG
IDACPMEGIIPAKYDEVLGLTDTEYQAVLACPVGYRHPEDKYAVLPKIRYAQEEIVAHIS
>Mature_219_residues
SDSTLTPEQLLHQLQWRYATKKFDPTQKIPTATWEALEQSLVLTPSSFGLQPWKFFVVQNSQIRQQLLEYSFGQTQVVDA
SHLVVLAIKKAANADDVDRYMQRTAEVRQVDIDSLTGFANVIKNFLANPHNPDFDPDEWATRQVYIALGQLMTSAAVLGI
DACPMEGIIPAKYDEVLGLTDTEYQAVLACPVGYRHPEDKYAVLPKIRYAQEEIVAHIS

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=205, Percent_Identity=32.6829268292683, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24798; Mature: 24667

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSTLTPEQLLHQLQWRYATKKFDPTQKIPTATWEALEQSLVLTPSSFGLQPWKFFVVQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCEEEEEEE
NSQIRQQLLEYSFGQTQVVDASHLVVLAIKKAANADDVDRYMQRTAEVRQVDIDSLTGFA
CHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHH
NVIKNFLANPHNPDFDPDEWATRQVYIALGQLMTSAAVLGIDACPMEGIIPAKYDEVLGL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
TDTEYQAVLACPVGYRHPEDKYAVLPKIRYAQEEIVAHIS
CCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSTLTPEQLLHQLQWRYATKKFDPTQKIPTATWEALEQSLVLTPSSFGLQPWKFFVVQ
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCEEEEEEE
NSQIRQQLLEYSFGQTQVVDASHLVVLAIKKAANADDVDRYMQRTAEVRQVDIDSLTGFA
CHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHH
NVIKNFLANPHNPDFDPDEWATRQVYIALGQLMTSAAVLGIDACPMEGIIPAKYDEVLGL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
TDTEYQAVLACPVGYRHPEDKYAVLPKIRYAQEEIVAHIS
CCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]