Click here to switch to the map view.

The map label for this gene is nirA [H]

Identifier: 158336122

GI number: 158336122

Start: 3010232

End: 3012169

Strand: Direct

Name: nirA [H]

Synonym: AM1_2984

Alternate gene names: 158336122

Gene position: 3010232-3012169 (Clockwise)

Preceding gene: 158336119

Following gene: 158336123

Centisome position: 46.28

GC content: 51.44

Gene sequence:

>1938_bases
GTGACTAATACCGCACCAGCTGCGCCCAAAATGAATAAGTTTGAGCGTTTCAAAGCGGAAAAAGATGGTTTGGCCGTTTT
ACCCGAGCTTTCCCACTTTGCTCAGATTGGTTGGGAAGCCATGGACGAAACTGACCTGACACAACGTCTCAAATGGTTAG
GGTTATTCTTCAGACCCGTTACACCTGGCAAATTCATGCTGCGGATGCGGATGCCCAACGGTATTCTCAGCGGCGGACAA
ATGCGGGTTCTGGCTGAAGTGATTCAGCGATACGGCGAAGATGGTTATGCCGATATCACCACTCGCCAAAATATTCAGTT
GCGCGGTATTCGCATCGAAGATATTCCTGATATTTTTAATCGGTTCCAGCAAGCCGGTCTAACCAGTGTGCAATCTGGGA
TGGATAACGTCCGCAATATTACCGGATCGCCTGTTGCGGGGCTGGATGCTGATGAGCTGATTGATACCCGAGGTCTGGTC
CGCAAAGTCCAGGACATGATCACCAATAATAGTCAGGGTAATCCAGCATTTACGAATCTGCCTCGGAAATTCAATATTGC
GATCGCAGGGGGTCGCGATAACTCCGTCCATGCTGAAATCAACGATATTGCCTTCGTCCCAGCATACAAAGATGGCAAGC
TTGGCTTTAACGTTTTGGTTGGCGGTTTCTTCTCTGCTAAGCGCTGCGCTCCTGCCATTTCCATGAACGTTTGGGTGGAC
CATACCCAAGTCGTGGATATCAGCGAAGCCATTCTCATTGTCTATCGGGACAACGGTTCCCGGGCCAATCGCCACCAATC
TCGGATGATGTGGCTGATCGACGACTGGGGAGTCGAACGCTTCCGGGCCGCCGTCGAAACCCAGATGGGTGTTACGCTCC
AAGCCGCTGCCGACAAGGATGAATTTGACTGGGACAAACGAGATCATCTCGGTGTCCATGCTCAAAAGCAGCCTGGCTTA
AACTTTGTAGGCATTCACGTTCCCGTCGGTCGGTTTTATGCCAACGATATGTTGGAATTAGCCCGCATGGCCGAGGTCTA
CGGCAGTGGCGAAATTCGTCTAACTGTCGAACAAAACGTCATTATTCCGAATATTCCTGACTCCCGCTTAGAATCTTTCT
TGGCAGAGCCTTTACTAGAAGTGTTCTCCATCAATCCTGACCCATTGGCCCGTGCCTTAGTCTCCTGTACTGGCAATCAG
TTCTGTAAGTTTGCACTGATTGAAACTAAAAATCGAGCCGTGGCTTTGATCGATGAACTAGAAGACGAATTAGACGTGCC
CAAACCGGTTCGCTTTCACTGGACCGGATGTCCTAATTCCTGCGGTCAGCCCCAAGTCGCTGATATCGGCCTGATGGGCA
CCAAAACTCGCAAGGATGGCAAGACCGTCGAAGCGGTGGACTTATTTATGGGTGGCAAAGTCGGTAAAGATGCTGTCCTC
GGTGAAAAAGTCCAAAAAGCCATCCCCTGTGATGAGCTCAAAGACGTCTTAAAAGATATTCTCATTCAGCAGTTTGATGC
TCAACCCCGATCCACTCCGCGTTCACCCAAAGGAACCGCCAGACTCGCCCGTCAACGCACTGTGCAAGACACTGTGAGTC
CAAACGGTTTTGCGGCTCCTGCGTCAGTCACTCCGGCAGCCCCGCCTGCCCCCACCCCGGCAGCCGCGGCCCAGCCGGCT
GTGGTGACCTTTGCAGCGTCTGGCAAGGAAGTCAATTGCGATGACAGCCAATCCTTATTGGAAATTGCGGAGGCTGCAGG
CGTTGAGATTGAAAGCAGTTGTCAATCTGGAAGCTGTGGCACCTGCATGGTCAAGCTGATTGAAGGTGAATTTCGATACG
AAGCCGATCCAGACGGCCTAGAAGATGCAGATGCAGCATCAGGGCATATCTTGACCTGCATTGCCTATCCAGTTGGAAAA
GTGGTGCTGGATGCCTAG

Upstream 100 bases:

>100_bases
AACGATCCCAATGGGAGTCGCTAAGTTAGTATCCCAATCGCAGAAATTGATTTTGATCAATTCTCTATTGGATTAAGACG
CGAGCTCAAGAGGAATTTAA

Downstream 100 bases:

>100_bases
TTTAAGTTTGCTCTTGGCTTATCGCGTTGTTTTTGGCTGATTTAGTTTTTTTATTTCTGTTTTGGAGAAGAATGCATGTT
AACGGAAATGTGGTCCTTTA

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MTNTAPAAPKMNKFERFKAEKDGLAVLPELSHFAQIGWEAMDETDLTQRLKWLGLFFRPVTPGKFMLRMRMPNGILSGGQ
MRVLAEVIQRYGEDGYADITTRQNIQLRGIRIEDIPDIFNRFQQAGLTSVQSGMDNVRNITGSPVAGLDADELIDTRGLV
RKVQDMITNNSQGNPAFTNLPRKFNIAIAGGRDNSVHAEINDIAFVPAYKDGKLGFNVLVGGFFSAKRCAPAISMNVWVD
HTQVVDISEAILIVYRDNGSRANRHQSRMMWLIDDWGVERFRAAVETQMGVTLQAAADKDEFDWDKRDHLGVHAQKQPGL
NFVGIHVPVGRFYANDMLELARMAEVYGSGEIRLTVEQNVIIPNIPDSRLESFLAEPLLEVFSINPDPLARALVSCTGNQ
FCKFALIETKNRAVALIDELEDELDVPKPVRFHWTGCPNSCGQPQVADIGLMGTKTRKDGKTVEAVDLFMGGKVGKDAVL
GEKVQKAIPCDELKDVLKDILIQQFDAQPRSTPRSPKGTARLARQRTVQDTVSPNGFAAPASVTPAAPPAPTPAAAAQPA
VVTFAASGKEVNCDDSQSLLEIAEAAGVEIESSCQSGSCGTCMVKLIEGEFRYEADPDGLEDADAASGHILTCIAYPVGK
VVLDA

Sequences:

>Translated_645_residues
MTNTAPAAPKMNKFERFKAEKDGLAVLPELSHFAQIGWEAMDETDLTQRLKWLGLFFRPVTPGKFMLRMRMPNGILSGGQ
MRVLAEVIQRYGEDGYADITTRQNIQLRGIRIEDIPDIFNRFQQAGLTSVQSGMDNVRNITGSPVAGLDADELIDTRGLV
RKVQDMITNNSQGNPAFTNLPRKFNIAIAGGRDNSVHAEINDIAFVPAYKDGKLGFNVLVGGFFSAKRCAPAISMNVWVD
HTQVVDISEAILIVYRDNGSRANRHQSRMMWLIDDWGVERFRAAVETQMGVTLQAAADKDEFDWDKRDHLGVHAQKQPGL
NFVGIHVPVGRFYANDMLELARMAEVYGSGEIRLTVEQNVIIPNIPDSRLESFLAEPLLEVFSINPDPLARALVSCTGNQ
FCKFALIETKNRAVALIDELEDELDVPKPVRFHWTGCPNSCGQPQVADIGLMGTKTRKDGKTVEAVDLFMGGKVGKDAVL
GEKVQKAIPCDELKDVLKDILIQQFDAQPRSTPRSPKGTARLARQRTVQDTVSPNGFAAPASVTPAAPPAPTPAAAAQPA
VVTFAASGKEVNCDDSQSLLEIAEAAGVEIESSCQSGSCGTCMVKLIEGEFRYEADPDGLEDADAASGHILTCIAYPVGK
VVLDA
>Mature_644_residues
TNTAPAAPKMNKFERFKAEKDGLAVLPELSHFAQIGWEAMDETDLTQRLKWLGLFFRPVTPGKFMLRMRMPNGILSGGQM
RVLAEVIQRYGEDGYADITTRQNIQLRGIRIEDIPDIFNRFQQAGLTSVQSGMDNVRNITGSPVAGLDADELIDTRGLVR
KVQDMITNNSQGNPAFTNLPRKFNIAIAGGRDNSVHAEINDIAFVPAYKDGKLGFNVLVGGFFSAKRCAPAISMNVWVDH
TQVVDISEAILIVYRDNGSRANRHQSRMMWLIDDWGVERFRAAVETQMGVTLQAAADKDEFDWDKRDHLGVHAQKQPGLN
FVGIHVPVGRFYANDMLELARMAEVYGSGEIRLTVEQNVIIPNIPDSRLESFLAEPLLEVFSINPDPLARALVSCTGNQF
CKFALIETKNRAVALIDELEDELDVPKPVRFHWTGCPNSCGQPQVADIGLMGTKTRKDGKTVEAVDLFMGGKVGKDAVLG
EKVQKAIPCDELKDVLKDILIQQFDAQPRSTPRSPKGTARLARQRTVQDTVSPNGFAAPASVTPAAPPAPTPAAAAQPAV
VTFAASGKEVNCDDSQSLLEIAEAAGVEIESSCQSGSCGTCMVKLIEGEFRYEADPDGLEDADAASGHILTCIAYPVGKV
VLDA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:
GO:0009055: Ferredoxin-nitrite reductase
GO:0016491: Ferredoxin-nitrite reductase
GO:0020037: Ferredoxin-nitrite reductase
GO:0046872: Ferredoxin-nitrite reductase
GO:0051536: Ferredoxin-nitrite reductase
GO:0051537: Ferredoxin-nitrite reductase
GO:0051539: Ferredoxin-nitrite reductase
GO:0055114: Ferredoxin-nitrite reductase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=430, Percent_Identity=24.8837209302326, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322597, Length=514, Percent_Identity=23.9299610894942, Blast_Score=98, Evalue=4e-21,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 70397; Mature: 70266

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00365 NIR_SIR ; PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTAPAAPKMNKFERFKAEKDGLAVLPELSHFAQIGWEAMDETDLTQRLKWLGLFFRPV
CCCCCCCCCCCHHHHHHHCCCCCCEECHHHHHHHHHCHHHCCHHHHHHHHHHHHHHEECC
TPGKFMLRMRMPNGILSGGQMRVLAEVIQRYGEDGYADITTRQNIQLRGIRIEDIPDIFN
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEECEECCCEEEEEEEECCHHHHHH
RFQQAGLTSVQSGMDNVRNITGSPVAGLDADELIDTRGLVRKVQDMITNNSQGNPAFTNL
HHHHHCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRKFNIAIAGGRDNSVHAEINDIAFVPAYKDGKLGFNVLVGGFFSAKRCAPAISMNVWVD
CCEEEEEEECCCCCCEEEEECCEEEEECCCCCCCCEEEEEECHHHHHHCCCEEEEEEEEC
HTQVVDISEAILIVYRDNGSRANRHQSRMMWLIDDWGVERFRAAVETQMGVTLQAAADKD
CEEEEECCCEEEEEECCCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHCCEEEEECCCCC
EFDWDKRDHLGVHAQKQPGLNFVGIHVPVGRFYANDMLELARMAEVYGSGEIRLTVEQNV
CCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCE
IIPNIPDSRLESFLAEPLLEVFSINPDPLARALVSCTGNQFCKFALIETKNRAVALIDEL
EECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHH
EDELDVPKPVRFHWTGCPNSCGQPQVADIGLMGTKTRKDGKTVEAVDLFMGGKVGKDAVL
HHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEHHHHCCCCCCHHHH
GEKVQKAIPCDELKDVLKDILIQQFDAQPRSTPRSPKGTARLARQRTVQDTVSPNGFAAP
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
ASVTPAAPPAPTPAAAAQPAVVTFAASGKEVNCDDSQSLLEIAEAAGVEIESSCQSGSCG
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCH
TCMVKLIEGEFRYEADPDGLEDADAASGHILTCIAYPVGKVVLDA
HHHHEECCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECC
>Mature Secondary Structure 
TNTAPAAPKMNKFERFKAEKDGLAVLPELSHFAQIGWEAMDETDLTQRLKWLGLFFRPV
CCCCCCCCCCHHHHHHHCCCCCCEECHHHHHHHHHCHHHCCHHHHHHHHHHHHHHEECC
TPGKFMLRMRMPNGILSGGQMRVLAEVIQRYGEDGYADITTRQNIQLRGIRIEDIPDIFN
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEECEECCCEEEEEEEECCHHHHHH
RFQQAGLTSVQSGMDNVRNITGSPVAGLDADELIDTRGLVRKVQDMITNNSQGNPAFTNL
HHHHHCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRKFNIAIAGGRDNSVHAEINDIAFVPAYKDGKLGFNVLVGGFFSAKRCAPAISMNVWVD
CCEEEEEEECCCCCCEEEEECCEEEEECCCCCCCCEEEEEECHHHHHHCCCEEEEEEEEC
HTQVVDISEAILIVYRDNGSRANRHQSRMMWLIDDWGVERFRAAVETQMGVTLQAAADKD
CEEEEECCCEEEEEECCCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHCCEEEEECCCCC
EFDWDKRDHLGVHAQKQPGLNFVGIHVPVGRFYANDMLELARMAEVYGSGEIRLTVEQNV
CCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCE
IIPNIPDSRLESFLAEPLLEVFSINPDPLARALVSCTGNQFCKFALIETKNRAVALIDEL
EECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHH
EDELDVPKPVRFHWTGCPNSCGQPQVADIGLMGTKTRKDGKTVEAVDLFMGGKVGKDAVL
HHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEHHHHCCCCCCHHHH
GEKVQKAIPCDELKDVLKDILIQQFDAQPRSTPRSPKGTARLARQRTVQDTVSPNGFAAP
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
ASVTPAAPPAPTPAAAAQPAVVTFAASGKEVNCDDSQSLLEIAEAAGVEIESSCQSGSCG
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCH
TCMVKLIEGEFRYEADPDGLEDADAASGHILTCIAYPVGKVVLDA
HHHHEECCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766873 [H]