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The map label for this gene is miaE [H]

Identifier: 158336095

GI number: 158336095

Start: 2986086

End: 2986697

Strand: Reverse

Name: miaE [H]

Synonym: AM1_2957

Alternate gene names: 158336095

Gene position: 2986697-2986086 (Counterclockwise)

Preceding gene: 158336097

Following gene: 158336094

Centisome position: 45.92

GC content: 56.86

Gene sequence:

>612_bases
TTGCCCACTGATGCTCCTGAGCGGGCGCAACCCACGATCAAGTTTTTGCAGCAACCGACGCGCTCGGACTGGATTGACCA
GGCCCTGCAGAATCTAGACACGATTTTGCTGGATCACTCCCACTGCGAGCGCAAGGCGGCGGGGGTGGCGGTGAATATGA
TGTTTCGCTATCCGTCTTATACCAAGCTGGTGCGGACGCTGACGGCGATTGCTAGGGAAGAGCTGGAGCATTTTGAGCTG
GTTAATCAGTGGCTCGATCGGCGGGGCATTGCTTTAGCGCCCTTGCCAGCCCCGCCCTATGGCTCGAAGCTCAGTGCCCA
AGTTCGCCGAGATGAGCCAGAGCGGCTGTTGGATATGCTGCTGGTCTCTGGCTTGATTGAAGCCCGCAGCCATGAACGGT
TGGGGCTGTTGGCTCAGCACCTCCCCGATCCAGATTTGGCGAAGTTTTATCGCAGTTTAATGGCATCGGAAGCCCGACAC
TATGGAGCCTATTGGGTCTTGGCAACCACGTATTTTGAAGTAGATGAGGTGACGGTCCGTCTGCAAGAACTAGCCACGGT
GGAAAGTGAGCTGCTAGCGACCCTCCACCCTCACCCCCGGATTCATAGCTAA

Upstream 100 bases:

>100_bases
CCCGTGAGTAAAAATCAAAATCCGATGGTAGGCAAGCTGTCTGCCAACGATTAAGGTAAAGAAGGGTAAATCCGATACCA
ATACGATGACTCACTCAACG

Downstream 100 bases:

>100_bases
TGCAGGACCAATTTGGTGAATTTGTGATTCGTGCCTGGCAGCCCTCGGATCGGCAGGCGGTGGCGGAAGTAATTCGGTCG
GTGTTGGCGGAGTATGGAAT

Product: tRNA hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MPTDAPERAQPTIKFLQQPTRSDWIDQALQNLDTILLDHSHCERKAAGVAVNMMFRYPSYTKLVRTLTAIAREELEHFEL
VNQWLDRRGIALAPLPAPPYGSKLSAQVRRDEPERLLDMLLVSGLIEARSHERLGLLAQHLPDPDLAKFYRSLMASEARH
YGAYWVLATTYFEVDEVTVRLQELATVESELLATLHPHPRIHS

Sequences:

>Translated_203_residues
MPTDAPERAQPTIKFLQQPTRSDWIDQALQNLDTILLDHSHCERKAAGVAVNMMFRYPSYTKLVRTLTAIAREELEHFEL
VNQWLDRRGIALAPLPAPPYGSKLSAQVRRDEPERLLDMLLVSGLIEARSHERLGLLAQHLPDPDLAKFYRSLMASEARH
YGAYWVLATTYFEVDEVTVRLQELATVESELLATLHPHPRIHS
>Mature_202_residues
PTDAPERAQPTIKFLQQPTRSDWIDQALQNLDTILLDHSHCERKAAGVAVNMMFRYPSYTKLVRTLTAIAREELEHFELV
NQWLDRRGIALAPLPAPPYGSKLSAQVRRDEPERLLDMLLVSGLIEARSHERLGLLAQHLPDPDLAKFYRSLMASEARHY
GAYWVLATTYFEVDEVTVRLQELATVESELLATLHPHPRIHS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 23215; Mature: 23084

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDAPERAQPTIKFLQQPTRSDWIDQALQNLDTILLDHSHCERKAAGVAVNMMFRYPSY
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCH
TKLVRTLTAIAREELEHFELVNQWLDRRGIALAPLPAPPYGSKLSAQVRRDEPERLLDML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHCCCHHHHHHHH
LVSGLIEARSHERLGLLAQHLPDPDLAKFYRSLMASEARHYGAYWVLATTYFEVDEVTVR
HHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHH
LQELATVESELLATLHPHPRIHS
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PTDAPERAQPTIKFLQQPTRSDWIDQALQNLDTILLDHSHCERKAAGVAVNMMFRYPSY
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCH
TKLVRTLTAIAREELEHFELVNQWLDRRGIALAPLPAPPYGSKLSAQVRRDEPERLLDML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHCCCHHHHHHHH
LVSGLIEARSHERLGLLAQHLPDPDLAKFYRSLMASEARHYGAYWVLATTYFEVDEVTVR
HHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHH
LQELATVESELLATLHPHPRIHS
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]