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The map label for this gene is ispF

Identifier: 158336056

GI number: 158336056

Start: 2950290

End: 2950775

Strand: Reverse

Name: ispF

Synonym: AM1_2915

Alternate gene names: 158336056

Gene position: 2950775-2950290 (Counterclockwise)

Preceding gene: 158336062

Following gene: 158336055

Centisome position: 45.37

GC content: 46.91

Gene sequence:

>486_bases
ATGACATCCATCCGCATTGGTAATGGTTACGATATCCATCAACTGGTTGAAGGTCGCCCGTTGATTCTTGGGGGGGTGCA
AATCGAGCATTCTCTAGGACTGAAAGGCCATAGTGATGCAGATGTGTTGACCCATGCAATTATGGATGCCCTATTAGGTG
CTTTGAGTTTAGGCGATATTGGTCATTATTTCCCACCGACCGATCCAAAATGGGCAGGAGCCGATAGTTTGAAGTTATTG
GAACAAGTTCACCAATTGATTCTGGATCGAGGCTGGCAGATCGGGAATATTGACTCGGTGGTGGTGGCTGAGCGCCCTAA
GCTTAAACCTCATATTGAAGCGATGCGCGATCGCATTAGCCAAGTCTTAAATCTCAGCCCGGAATTAATCGGTATAAAAG
CGACAACCAATGAAAAGCTCGGACCCGTAGGACAAGAGCAGGGTATTTGCTCCTATGCAGTAGCATTATTGACCTCAGAT
TCGTAG

Upstream 100 bases:

>100_bases
TCTTAGCAGCCTAACGATTAGTGCTGTAAATAGAGAGGTACCGTCATAGTATGGTACGAGAGCACTGAGCCGTTTTTCAA
TTGATTCCCGTAGTAAAACT

Downstream 100 bases:

>100_bases
ATGAGAGCTGAGTAAGATCTGCGATGACCGAGAACCTGGTACTAGGGATTGATTTAGGAGGGACAGCGATTAAGCTGGGT
TGCTGTAGCCCTCAAGGTAC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTSIRIGNGYDIHQLVEGRPLILGGVQIEHSLGLKGHSDADVLTHAIMDALLGALSLGDIGHYFPPTDPKWAGADSLKLL
EQVHQLILDRGWQIGNIDSVVVAERPKLKPHIEAMRDRISQVLNLSPELIGIKATTNEKLGPVGQEQGICSYAVALLTSD
S

Sequences:

>Translated_161_residues
MTSIRIGNGYDIHQLVEGRPLILGGVQIEHSLGLKGHSDADVLTHAIMDALLGALSLGDIGHYFPPTDPKWAGADSLKLL
EQVHQLILDRGWQIGNIDSVVVAERPKLKPHIEAMRDRISQVLNLSPELIGIKATTNEKLGPVGQEQGICSYAVALLTSD
S
>Mature_160_residues
TSIRIGNGYDIHQLVEGRPLILGGVQIEHSLGLKGHSDADVLTHAIMDALLGALSLGDIGHYFPPTDPKWAGADSLKLLE
QVHQLILDRGWQIGNIDSVVVAERPKLKPHIEAMRDRISQVLNLSPELIGIKATTNEKLGPVGQEQGICSYAVALLTSDS

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:
GO:0008299: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
GO:0008685: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
GO:0016114: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
GO:0016829: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
GO:0046872: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=48.0519480519481, Blast_Score=136, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_ACAM1 (B0CBC9)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517230.1
- ProteinModelPortal:   B0CBC9
- SMR:   B0CBC9
- GeneID:   5681723
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2915
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   AMAR329726:AM1_2915-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17370; Mature: 17239

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIRIGNGYDIHQLVEGRPLILGGVQIEHSLGLKGHSDADVLTHAIMDALLGALSLGDI
CCEEECCCCCCHHHHHCCCCEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GHYFPPTDPKWAGADSLKLLEQVHQLILDRGWQIGNIDSVVVAERPKLKPHIEAMRDRIS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
QVLNLSPELIGIKATTNEKLGPVGQEQGICSYAVALLTSDS
HHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TSIRIGNGYDIHQLVEGRPLILGGVQIEHSLGLKGHSDADVLTHAIMDALLGALSLGDI
CEEECCCCCCHHHHHCCCCEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GHYFPPTDPKWAGADSLKLLEQVHQLILDRGWQIGNIDSVVVAERPKLKPHIEAMRDRIS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
QVLNLSPELIGIKATTNEKLGPVGQEQGICSYAVALLTSDS
HHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA