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The map label for this gene is recJ [H]

Identifier: 158336032

GI number: 158336032

Start: 2930124

End: 2932511

Strand: Direct

Name: recJ [H]

Synonym: AM1_2891

Alternate gene names: 158336032

Gene position: 2930124-2932511 (Clockwise)

Preceding gene: 158336029

Following gene: 158336033

Centisome position: 45.05

GC content: 52.39

Gene sequence:

>2388_bases
GTGCCTAAACCATCTTTTCCACAGCAACGCTGGCAAATTGCTGATCCAGAGCACACTCTAGTAGACAAGATTGCGATCGC
AACTGGATTATCGCCTCTTTTGGCCGCAGTATTAGCCAACCAAGGGATAGGGTCTGTCGAAGCGGCTCAACAGTTTTTGG
CACCAGAGTCCCAGAGCTTACCCTCTCCTCTTGAAGCATTCCCGGATTTGGCTGCTAGCTTAACGTTGTTACAGGATGCG
ATCGCATCTCGACACAAAATTGCCATCTGTGGCGACTATGATGCAGACGGCATGACCAGCACGGCTCTACTACTGCGCGT
CCTGAGAGCATTGGGTGCTGACGTAGACTATGCGATACCAAGCCGCATGCAGGAAGGCTACGGCATTAACCAACGGATCG
TCGAGGAATTTGCGGCAGATAACGTGACGCTGGTGTTAACCGTAGACAACGGTATTGCCGCCTATGACCCTATTGTGAGA
GCCCGCGAACTGGGATTAACCGTCATCATCACGGATCACCATGATCTGCCGCCTCAGCTCCCCCCGGCCAACGCCATTCT
CAATCCCAAATTAGTGCCAGAAGACTCCCCTTATCGAGGGTTAGCAGGGGTGGGGGTCGCTTATGTCTTAGCCACCCTAC
TCGCCCAAGAGCAAGGTAATACCACCCTGATTACCCCTCTACTAGAACTGTTTACCCTGGGCACCATTGCTGATTTGGCT
CCCTTAGTGGGGGTCAATCGCCGCTGGCTACAGCGAGGTTTAGCCCTCTTGCCCCACTCTCGATTAGCAGGGGTACAGTC
TTTGATGCAGGTGTCGGGCTGTGTCTCTGCCGATCAAGCCCTCAAACCGGACGCTATTGGCTTCCGACTCGGTCCCCGCA
TCAATGCCGTGGGCCGGATTGCCGATCCACAAACGGTAATTGAGCTATTAACGACTGACGACCCAGGCATCGCGTTTGCC
CGAGCCATGCAGTGTGAGCAGGCTAACCAGCATCGACGTCAGCTCTGTGCCGACATTGAGATAGAGGCGATCGAACTCGT
TGAAACCCTTGTCCGACAGGGGAAAGCGCCGTGGCAACCAGAGGGCGATTGGGTGATTATGCTGGTTCATCCCGGTTGGC
ATCATGGCGTGATTGGGATTGTGGCGTCTCGGTTAGTGGAACGCTACGGACTACCGGTTTTTATTGCCACCTATGAAGCA
GAAGACCATACCCATATCCGTGGATCCGCCCGAGGGATTCCTGAATTTGATGTGTTTGCAGCCCTACAAGCCTGTGATGA
AGCCTTAGGTCGCTATGGGGGCCACCGAGCAGCAGGCGGATTTTCCTTAGCAACCGACCAGTTAGAGAAGATGCGATCGC
ACCTGCGTACCTACGCCCACCAAACCTTACAGATAGATCAGCTCAAGCCTCTGGTAACGGTCGATGCCCAAGCCAGCTTC
ACTCAGATCGATTGGGATCTCTACGAGCAAATTAATCAGCTGCATCCCTGCGGTATTGGCAATCCAGAACCGATTTTTTG
GACCGCCAATGTTGAAGTGGTAGAGCAGCGACCCGTGGGCAAGGACAAAAGCCATTTAAAGCTCACCCTAGCTCATACTG
AATCAGACGATCGGCAGCAGTTGATTAAAGGGATGGCATGGCGGTGGGGCACCTATTATCCTTTACCCCAGCGGATCGAC
GTTGCCTATCGCCTTAGAGAAAATACTTGGAACCAGCAAACCAATTTGGAGCTGGAAATTTTAGGGATTCGGGAGCCCGT
TGTAGACCCACCCCACTCACTATCTCGTACTCCAATTGTGGTTGCCCAAGCCGATAAAGGGGCACCCGTTGTTCGCCAGC
AAGTGGCCTTACACTGTCCAGAGCCCGACGTATCACCCACACTACTACAAGCCATAGAGTGGTCGAGCTTGGGGGATTGG
CCTCAAGTCCTACAAGGCATTAGCGGTCATATTCTCATGTATGGTTATCAGCGTCCCTATGTATCGACGACAACCCTGCC
CGGAACGGTGGAATACGACCGGCCTAGCCGTTCCTGTGACTGTCTAATTCTATGGTCTTTGCCGCCTTCATGGACCCATT
TACGCTGGTTATTAGCCTTGGCTCGGCCCCAGCAAATTTATATTCGCAACCATAGTCCAGCAATTTTGCCAGCAGAGGAA
TTAAAGGCAAAACTAAAGTTTGAAATAGCAAAAAACGGTGACGACCCCCTTAACTTACTGAACTTAGGCCAGCAATGGTG
GGTTGCACCGAGTACAATTGTTTCTGCTTTTAGAGAAATAGGTTACCCCTGCTCTGATTTCCCGGAGACGGGTGTGTTAG
AGCAGGAGTTAAAACGCTTGCAACGTTGGTATCATTGTTCAGCCAATCGATTGGCAGGGTTAACCTAG

Upstream 100 bases:

>100_bases
GGTTCGTCAAATTTAGGGATTATTAATTTATTGATCTCGGATCGGGGGGTGGAGCCCCACCATGACCGATGGTGGAAGTG
GTCTAACGCAATTTTTCCCT

Downstream 100 bases:

>100_bases
GTTGCGTTACTCCGAAGAGCAACCAATAGCACGACAACTGGGCCAGCAATGACGACAGCGCCAAGCATAGATAGTTGAAG
TACAACTTGTAAATTAAGTG

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MPKPSFPQQRWQIADPEHTLVDKIAIATGLSPLLAAVLANQGIGSVEAAQQFLAPESQSLPSPLEAFPDLAASLTLLQDA
IASRHKIAICGDYDADGMTSTALLLRVLRALGADVDYAIPSRMQEGYGINQRIVEEFAADNVTLVLTVDNGIAAYDPIVR
ARELGLTVIITDHHDLPPQLPPANAILNPKLVPEDSPYRGLAGVGVAYVLATLLAQEQGNTTLITPLLELFTLGTIADLA
PLVGVNRRWLQRGLALLPHSRLAGVQSLMQVSGCVSADQALKPDAIGFRLGPRINAVGRIADPQTVIELLTTDDPGIAFA
RAMQCEQANQHRRQLCADIEIEAIELVETLVRQGKAPWQPEGDWVIMLVHPGWHHGVIGIVASRLVERYGLPVFIATYEA
EDHTHIRGSARGIPEFDVFAALQACDEALGRYGGHRAAGGFSLATDQLEKMRSHLRTYAHQTLQIDQLKPLVTVDAQASF
TQIDWDLYEQINQLHPCGIGNPEPIFWTANVEVVEQRPVGKDKSHLKLTLAHTESDDRQQLIKGMAWRWGTYYPLPQRID
VAYRLRENTWNQQTNLELEILGIREPVVDPPHSLSRTPIVVAQADKGAPVVRQQVALHCPEPDVSPTLLQAIEWSSLGDW
PQVLQGISGHILMYGYQRPYVSTTTLPGTVEYDRPSRSCDCLILWSLPPSWTHLRWLLALARPQQIYIRNHSPAILPAEE
LKAKLKFEIAKNGDDPLNLLNLGQQWWVAPSTIVSAFREIGYPCSDFPETGVLEQELKRLQRWYHCSANRLAGLT

Sequences:

>Translated_795_residues
MPKPSFPQQRWQIADPEHTLVDKIAIATGLSPLLAAVLANQGIGSVEAAQQFLAPESQSLPSPLEAFPDLAASLTLLQDA
IASRHKIAICGDYDADGMTSTALLLRVLRALGADVDYAIPSRMQEGYGINQRIVEEFAADNVTLVLTVDNGIAAYDPIVR
ARELGLTVIITDHHDLPPQLPPANAILNPKLVPEDSPYRGLAGVGVAYVLATLLAQEQGNTTLITPLLELFTLGTIADLA
PLVGVNRRWLQRGLALLPHSRLAGVQSLMQVSGCVSADQALKPDAIGFRLGPRINAVGRIADPQTVIELLTTDDPGIAFA
RAMQCEQANQHRRQLCADIEIEAIELVETLVRQGKAPWQPEGDWVIMLVHPGWHHGVIGIVASRLVERYGLPVFIATYEA
EDHTHIRGSARGIPEFDVFAALQACDEALGRYGGHRAAGGFSLATDQLEKMRSHLRTYAHQTLQIDQLKPLVTVDAQASF
TQIDWDLYEQINQLHPCGIGNPEPIFWTANVEVVEQRPVGKDKSHLKLTLAHTESDDRQQLIKGMAWRWGTYYPLPQRID
VAYRLRENTWNQQTNLELEILGIREPVVDPPHSLSRTPIVVAQADKGAPVVRQQVALHCPEPDVSPTLLQAIEWSSLGDW
PQVLQGISGHILMYGYQRPYVSTTTLPGTVEYDRPSRSCDCLILWSLPPSWTHLRWLLALARPQQIYIRNHSPAILPAEE
LKAKLKFEIAKNGDDPLNLLNLGQQWWVAPSTIVSAFREIGYPCSDFPETGVLEQELKRLQRWYHCSANRLAGLT
>Mature_794_residues
PKPSFPQQRWQIADPEHTLVDKIAIATGLSPLLAAVLANQGIGSVEAAQQFLAPESQSLPSPLEAFPDLAASLTLLQDAI
ASRHKIAICGDYDADGMTSTALLLRVLRALGADVDYAIPSRMQEGYGINQRIVEEFAADNVTLVLTVDNGIAAYDPIVRA
RELGLTVIITDHHDLPPQLPPANAILNPKLVPEDSPYRGLAGVGVAYVLATLLAQEQGNTTLITPLLELFTLGTIADLAP
LVGVNRRWLQRGLALLPHSRLAGVQSLMQVSGCVSADQALKPDAIGFRLGPRINAVGRIADPQTVIELLTTDDPGIAFAR
AMQCEQANQHRRQLCADIEIEAIELVETLVRQGKAPWQPEGDWVIMLVHPGWHHGVIGIVASRLVERYGLPVFIATYEAE
DHTHIRGSARGIPEFDVFAALQACDEALGRYGGHRAAGGFSLATDQLEKMRSHLRTYAHQTLQIDQLKPLVTVDAQASFT
QIDWDLYEQINQLHPCGIGNPEPIFWTANVEVVEQRPVGKDKSHLKLTLAHTESDDRQQLIKGMAWRWGTYYPLPQRIDV
AYRLRENTWNQQTNLELEILGIREPVVDPPHSLSRTPIVVAQADKGAPVVRQQVALHCPEPDVSPTLLQAIEWSSLGDWP
QVLQGISGHILMYGYQRPYVSTTTLPGTVEYDRPSRSCDCLILWSLPPSWTHLRWLLALARPQQIYIRNHSPAILPAEEL
KAKLKFEIAKNGDDPLNLLNLGQQWWVAPSTIVSAFREIGYPCSDFPETGVLEQELKRLQRWYHCSANRLAGLT

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:
GO:0003676: Single-stranded-DNA-specific exonuclease RecJ
GO:0004527: Single-stranded-DNA-specific exonuclease RecJ
GO:0006281: Single-stranded-DNA-specific exonuclease RecJ
GO:0006310: Single-stranded-DNA-specific exonuclease RecJ
GO:0008409: Single-stranded-DNA-specific exonuclease RecJ
GO:0016787: Single-stranded-DNA-specific exonuclease RecJ
GO:0030145: Single-stranded-DNA-specific exonuclease RecJ

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=573, Percent_Identity=32.4607329842932, Blast_Score=245, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 87859; Mature: 87727

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPSFPQQRWQIADPEHTLVDKIAIATGLSPLLAAVLANQGIGSVEAAQQFLAPESQSL
CCCCCCCHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
PSPLEAFPDLAASLTLLQDAIASRHKIAICGDYDADGMTSTALLLRVLRALGADVDYAIP
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCH
SRMQEGYGINQRIVEEFAADNVTLVLTVDNGIAAYDPIVRARELGLTVIITDHHDLPPQL
HHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCC
PPANAILNPKLVPEDSPYRGLAGVGVAYVLATLLAQEQGNTTLITPLLELFTLGTIADLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHH
PLVGVNRRWLQRGLALLPHSRLAGVQSLMQVSGCVSADQALKPDAIGFRLGPRINAVGRI
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCC
ADPQTVIELLTTDDPGIAFARAMQCEQANQHRRQLCADIEIEAIELVETLVRQGKAPWQP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
EGDWVIMLVHPGWHHGVIGIVASRLVERYGLPVFIATYEAEDHTHIRGSARGIPEFDVFA
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCCCCCHHHHHH
ALQACDEALGRYGGHRAAGGFSLATDQLEKMRSHLRTYAHQTLQIDQLKPLVTVDAQASF
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCC
TQIDWDLYEQINQLHPCGIGNPEPIFWTANVEVVEQRPVGKDKSHLKLTLAHTESDDRQQ
EECCHHHHHHHHHCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCEEEEEEECCCHHHHH
LIKGMAWRWGTYYPLPQRIDVAYRLRENTWNQQTNLELEILGIREPVVDPPHSLSRTPIV
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEE
VAQADKGAPVVRQQVALHCPEPDVSPTLLQAIEWSSLGDWPQVLQGISGHILMYGYQRPY
EEECCCCCHHHHHHHHEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCC
VSTTTLPGTVEYDRPSRSCDCLILWSLPPSWTHLRWLLALARPQQIYIRNHSPAILPAEE
EEECCCCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
LKAKLKFEIAKNGDDPLNLLNLGQQWWVAPSTIVSAFREIGYPCSDFPETGVLEQELKRL
HHCEEEEEEECCCCCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QRWYHCSANRLAGLT
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
PKPSFPQQRWQIADPEHTLVDKIAIATGLSPLLAAVLANQGIGSVEAAQQFLAPESQSL
CCCCCCHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
PSPLEAFPDLAASLTLLQDAIASRHKIAICGDYDADGMTSTALLLRVLRALGADVDYAIP
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCH
SRMQEGYGINQRIVEEFAADNVTLVLTVDNGIAAYDPIVRARELGLTVIITDHHDLPPQL
HHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCC
PPANAILNPKLVPEDSPYRGLAGVGVAYVLATLLAQEQGNTTLITPLLELFTLGTIADLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHH
PLVGVNRRWLQRGLALLPHSRLAGVQSLMQVSGCVSADQALKPDAIGFRLGPRINAVGRI
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCC
ADPQTVIELLTTDDPGIAFARAMQCEQANQHRRQLCADIEIEAIELVETLVRQGKAPWQP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
EGDWVIMLVHPGWHHGVIGIVASRLVERYGLPVFIATYEAEDHTHIRGSARGIPEFDVFA
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCCCCCHHHHHH
ALQACDEALGRYGGHRAAGGFSLATDQLEKMRSHLRTYAHQTLQIDQLKPLVTVDAQASF
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCC
TQIDWDLYEQINQLHPCGIGNPEPIFWTANVEVVEQRPVGKDKSHLKLTLAHTESDDRQQ
EECCHHHHHHHHHCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCEEEEEEECCCHHHHH
LIKGMAWRWGTYYPLPQRIDVAYRLRENTWNQQTNLELEILGIREPVVDPPHSLSRTPIV
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEE
VAQADKGAPVVRQQVALHCPEPDVSPTLLQAIEWSSLGDWPQVLQGISGHILMYGYQRPY
EEECCCCCHHHHHHHHEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCC
VSTTTLPGTVEYDRPSRSCDCLILWSLPPSWTHLRWLLALARPQQIYIRNHSPAILPAEE
EEECCCCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
LKAKLKFEIAKNGDDPLNLLNLGQQWWVAPSTIVSAFREIGYPCSDFPETGVLEQELKRL
HHCEEEEEEECCCCCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QRWYHCSANRLAGLT
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]