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The map label for this gene is murF [H]

Identifier: 158335995

GI number: 158335995

Start: 2895299

End: 2896645

Strand: Direct

Name: murF [H]

Synonym: AM1_2853

Alternate gene names: 158335995

Gene position: 2895299-2896645 (Clockwise)

Preceding gene: 158335994

Following gene: 158335998

Centisome position: 44.52

GC content: 53.82

Gene sequence:

>1347_bases
ATGACCTTGCAGGCTCCCCTTGCCCAACTAATCGAGATTCTGGCCGCACAGCCCCTTCATATCCCTGGTGCTTTAACCTC
CCAGCAGGTTCAAGGCGTTAATACGGACACTCGCAGCCTGAAACCCCAGGAAATTTTTCTGGCCTTCTCGGGGGAGAACT
TTGATGGTCATCGGTTTGCAGAATCGGCAGTGCAGCAGGGCGCAATCGCCGCTATTGTCGATCACCCCATCGATGCGGCT
ACTCCCCAGTTAGTGGTTCCAGATACCCTGGTGGCCTATCAACGCTTGGGGCAATGGTGGCGACAGCAGTTTCAAATCCC
GATTATTGCCATTACAGGCTCTGTCGGCAAAACCACCACTAAAGAGCTGATTTCGGCGGTCCTCGGCACGGCTGGATCCG
TCCTCAAAACCGAAGCCAACTACAACAACGAAATTGGTGTTCCCAAAACCTTGTTAGGATTAACCGCCGATCATGACTAT
GCAGTTGTGGAAATGGGGATGCGGGGGCCAGGAGAAATCGCAGAACTATCTCAGATTGCCCAACCCACCATTGGTGTAAT
CACCAATGTCGGCACTGCTCATATTGGTCGTCTAGGGTCTAGAGAAGCCATTGCCCAAGCCAAATGTGAGCTACTGGCCC
AGATGCCTGCAGAGAGTCTGGCGGTTCTCAATGCCGATAATTCTTTACTGATTGAAACGGCTCAAACCGTGTGGTCGGGG
GCTCAAGTCACCTATGGCCTCGACTCTGGCGATTTACAAGGTCAGCTAGCAGATCAAACACTACAGGTAGCGGATCATTC
CTTTCCATTACCCCTGCCAGGGGCTCATAATGCCTTGAACTATTTAGCCGCCCTGGCCATCGCCCAGCATCTCCATTTGG
ATCTCGCTCCCCTACAGCAGGGTATTTCCCTAGAACTTCCCTCCGGTCGAGCTCGTAAACTCACCCTGCCCAACGATGTG
GTGGTTCTAGATGAGACCTATAACGCTGGCCTGGAGTCCATGCAGGCAGCTCTCCATCTACTCGCCCAAACACCCGGTGA
GCGGCGAATTGCGGTACTGGGGCCGATGAAAGAATTGGGAGAATATGCCGCCGAACTCCATCGCCAAGTGGGCCGTTTAG
TCCAGACCCTAAACATCGATCAGCTTTTGATCCTCGATCAAGGACCGGAAGGAACCGCCTTAGCAGCAGGAACAACCACG
GTCCCCACGGAGCAGTTTGCTGAACACCAAGCCCTGATGGACTATCTCGATCAGCATATTCAGCCGGGCGATCGTCTTCT
CTGCAAAGCGTCCCACTCCGTGGGGCTAGACCGCATTGTCGACCATTTCACCCAAGTCAAGGCTTAG

Upstream 100 bases:

>100_bases
GGCTGGACCGGACTACGGGACTCTTCCAACTAGGGCCATCAGCAGACGGCCAGCAAGGTTTAGGGGATAATGACTAGCAG
TCTTTAGAACATCCTCTCCC

Downstream 100 bases:

>100_bases
GCGCTTACGAGGCTAACGCCTGACGCTGGGCCTCGATTAACTGTTGAATGCCAATTTCTGCCAGGTCTAACATTTGATTG
AGCTGGGGACGAGGCAAAGT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTLQAPLAQLIEILAAQPLHIPGALTSQQVQGVNTDTRSLKPQEIFLAFSGENFDGHRFAESAVQQGAIAAIVDHPIDAA
TPQLVVPDTLVAYQRLGQWWRQQFQIPIIAITGSVGKTTTKELISAVLGTAGSVLKTEANYNNEIGVPKTLLGLTADHDY
AVVEMGMRGPGEIAELSQIAQPTIGVITNVGTAHIGRLGSREAIAQAKCELLAQMPAESLAVLNADNSLLIETAQTVWSG
AQVTYGLDSGDLQGQLADQTLQVADHSFPLPLPGAHNALNYLAALAIAQHLHLDLAPLQQGISLELPSGRARKLTLPNDV
VVLDETYNAGLESMQAALHLLAQTPGERRIAVLGPMKELGEYAAELHRQVGRLVQTLNIDQLLILDQGPEGTALAAGTTT
VPTEQFAEHQALMDYLDQHIQPGDRLLCKASHSVGLDRIVDHFTQVKA

Sequences:

>Translated_448_residues
MTLQAPLAQLIEILAAQPLHIPGALTSQQVQGVNTDTRSLKPQEIFLAFSGENFDGHRFAESAVQQGAIAAIVDHPIDAA
TPQLVVPDTLVAYQRLGQWWRQQFQIPIIAITGSVGKTTTKELISAVLGTAGSVLKTEANYNNEIGVPKTLLGLTADHDY
AVVEMGMRGPGEIAELSQIAQPTIGVITNVGTAHIGRLGSREAIAQAKCELLAQMPAESLAVLNADNSLLIETAQTVWSG
AQVTYGLDSGDLQGQLADQTLQVADHSFPLPLPGAHNALNYLAALAIAQHLHLDLAPLQQGISLELPSGRARKLTLPNDV
VVLDETYNAGLESMQAALHLLAQTPGERRIAVLGPMKELGEYAAELHRQVGRLVQTLNIDQLLILDQGPEGTALAAGTTT
VPTEQFAEHQALMDYLDQHIQPGDRLLCKASHSVGLDRIVDHFTQVKA
>Mature_447_residues
TLQAPLAQLIEILAAQPLHIPGALTSQQVQGVNTDTRSLKPQEIFLAFSGENFDGHRFAESAVQQGAIAAIVDHPIDAAT
PQLVVPDTLVAYQRLGQWWRQQFQIPIIAITGSVGKTTTKELISAVLGTAGSVLKTEANYNNEIGVPKTLLGLTADHDYA
VVEMGMRGPGEIAELSQIAQPTIGVITNVGTAHIGRLGSREAIAQAKCELLAQMPAESLAVLNADNSLLIETAQTVWSGA
QVTYGLDSGDLQGQLADQTLQVADHSFPLPLPGAHNALNYLAALAIAQHLHLDLAPLQQGISLELPSGRARKLTLPNDVV
VLDETYNAGLESMQAALHLLAQTPGERRIAVLGPMKELGEYAAELHRQVGRLVQTLNIDQLLILDQGPEGTALAAGTTTV
PTEQFAEHQALMDYLDQHIQPGDRLLCKASHSVGLDRIVDHFTQVKA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=452, Percent_Identity=35.6194690265487, Blast_Score=228, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47867; Mature: 47736

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQAPLAQLIEILAAQPLHIPGALTSQQVQGVNTDTRSLKPQEIFLAFSGENFDGHRFA
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHH
ESAVQQGAIAAIVDHPIDAATPQLVVPDTLVAYQRLGQWWRQQFQIPIIAITGSVGKTTT
HHHHHCCCEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
KELISAVLGTAGSVLKTEANYNNEIGVPKTLLGLTADHDYAVVEMGMRGPGEIAELSQIA
HHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
QPTIGVITNVGTAHIGRLGSREAIAQAKCELLAQMPAESLAVLNADNSLLIETAQTVWSG
CCHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEEEHHHHHCC
AQVTYGLDSGDLQGQLADQTLQVADHSFPLPLPGAHNALNYLAALAIAQHLHLDLAPLQQ
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHC
GISLELPSGRARKLTLPNDVVVLDETYNAGLESMQAALHLLAQTPGERRIAVLGPMKELG
CCEEECCCCCCEEEECCCCEEEEECCHHCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHH
EYAAELHRQVGRLVQTLNIDQLLILDQGPEGTALAAGTTTVPTEQFAEHQALMDYLDQHI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHC
QPGDRLLCKASHSVGLDRIVDHFTQVKA
CCCCCEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TLQAPLAQLIEILAAQPLHIPGALTSQQVQGVNTDTRSLKPQEIFLAFSGENFDGHRFA
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHH
ESAVQQGAIAAIVDHPIDAATPQLVVPDTLVAYQRLGQWWRQQFQIPIIAITGSVGKTTT
HHHHHCCCEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
KELISAVLGTAGSVLKTEANYNNEIGVPKTLLGLTADHDYAVVEMGMRGPGEIAELSQIA
HHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
QPTIGVITNVGTAHIGRLGSREAIAQAKCELLAQMPAESLAVLNADNSLLIETAQTVWSG
CCHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEEEHHHHHCC
AQVTYGLDSGDLQGQLADQTLQVADHSFPLPLPGAHNALNYLAALAIAQHLHLDLAPLQQ
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHC
GISLELPSGRARKLTLPNDVVVLDETYNAGLESMQAALHLLAQTPGERRIAVLGPMKELG
CCEEECCCCCCEEEECCCCEEEEECCHHCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHH
EYAAELHRQVGRLVQTLNIDQLLILDQGPEGTALAAGTTTVPTEQFAEHQALMDYLDQHI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHC
QPGDRLLCKASHSVGLDRIVDHFTQVKA
CCCCCEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]