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The map label for this gene is galE [C]

Identifier: 158335954

GI number: 158335954

Start: 2849990

End: 2851153

Strand: Reverse

Name: galE [C]

Synonym: AM1_2812

Alternate gene names: 158335954

Gene position: 2851153-2849990 (Counterclockwise)

Preceding gene: 158335955

Following gene: 158335950

Centisome position: 43.84

GC content: 45.7

Gene sequence:

>1164_bases
ATGAACATTGTAATATTGGGCGGTGATGGGTTCTGCGGCTGGCCAACTGCGCTACATCTATCCAAACTTGGCCATGATGT
TGTCATTGTGGATAATTTATCCAGGCGCAACATTGATAATGAGTTGGAGACCAACTCGTTAACCCCCATTAGTCCGATTT
CGATTCGCCTGAAAACCTGGCAAGACATAACAGGCAAGCATATTCAGTTCTATAACTTCGATATCGCGGCGGAATACGAT
CGGCTCTTAAATCTGTTTTGCACCTATCGCCCTGATGTGGTTGTGCATTTTGCTGAGCAACGGGCTGCACCGTATTCCAT
GAAGTCATCCCGGCAAAAGCGCTACACGGTTAATAACAATCTCAATGCGACCAATAACGTTTTAGCCGCCATCGTTGAAT
CTGGCCTGGATATCCATGTGGTTCACCTGGGCACCATGGGCGTTTATGGCTATGGTACTGCTGGCATGAAGATTCCTGAA
GGCTATCTTGATGTTCAGGTGGTGATTGATACCGGTGAGGTGGTTGAAAAGCAAATCCTCTATCCTGCGGATCCGGGCAG
TGTTTATCACATGACCAAAACCCAGGATCAGCTCTTCTTCTACTACTACAACAAGAATGACAAGATTCGAGTCACCGACT
TGCACCAAGGTGTAGTCTGGGGAACGAATACAGAAGATACAAAGCAGGATGAACGCCTGATTAATCGCTTTGATTATGAC
GGTGATTATGGGACTGTCCTGAACCGATTTCTGATGCAGGCCGCCATTGGCCACCCCTTAACAGTGCATGGTACGGGCGG
TCAGACCCGAGCATTTATTCATATTCAGGACACTGTTAAATGTATTCAACTAGCCGTTGAGAACCCCCCTGAAGCTGGAG
AGCGGGTCAAAATATTTAACCAAATGACTGAAACCCACCGGGTGAGGGATTTGGCCAAGATTATTGCAGATATTACTGGG
GCTAAGATTGACTACTTAGAAAATCCCAGAACAGAGGCAGCAGAAAACGAATTATTTGTTGAGAATAAGTGCTTTATGGA
TTTAGGTCTGCAACCGACGAAGTTAGATGTTGGCCTGATGAAAGAAGTAACTGAAATTGCAGCGAAGTATAAAGATCGGT
GCGATCACTCCAAAATTCTATGTACGTCTAAGTGGCGTAAATAG

Upstream 100 bases:

>100_bases
TATCTAACGGTTGCTGAAAAGGGTGTGTCGTTGACTGACCAGCAATTCTGAAGATTCAAATTCTCGTCTGTAATTGAGAC
ATTCGGTTTAGGGACAAAGC

Downstream 100 bases:

>100_bases
GCAGATTCAATACTACCTAGCTGAATTTGAGCAAATAGCGACGGTCTTTTTAGGCCGTCGCTATTTGCTTAAGGAGTCAA
GCTGGCGTCACTAGTCTTTT

Product: UDP-sulfoquinovose synthase

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Epimerase; UDP Sulfoquinovose Synthase; Nucleoside-Diphosphate-Sugar Epimerase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNIVILGGDGFCGWPTALHLSKLGHDVVIVDNLSRRNIDNELETNSLTPISPISIRLKTWQDITGKHIQFYNFDIAAEYD
RLLNLFCTYRPDVVVHFAEQRAAPYSMKSSRQKRYTVNNNLNATNNVLAAIVESGLDIHVVHLGTMGVYGYGTAGMKIPE
GYLDVQVVIDTGEVVEKQILYPADPGSVYHMTKTQDQLFFYYYNKNDKIRVTDLHQGVVWGTNTEDTKQDERLINRFDYD
GDYGTVLNRFLMQAAIGHPLTVHGTGGQTRAFIHIQDTVKCIQLAVENPPEAGERVKIFNQMTETHRVRDLAKIIADITG
AKIDYLENPRTEAAENELFVENKCFMDLGLQPTKLDVGLMKEVTEIAAKYKDRCDHSKILCTSKWRK

Sequences:

>Translated_387_residues
MNIVILGGDGFCGWPTALHLSKLGHDVVIVDNLSRRNIDNELETNSLTPISPISIRLKTWQDITGKHIQFYNFDIAAEYD
RLLNLFCTYRPDVVVHFAEQRAAPYSMKSSRQKRYTVNNNLNATNNVLAAIVESGLDIHVVHLGTMGVYGYGTAGMKIPE
GYLDVQVVIDTGEVVEKQILYPADPGSVYHMTKTQDQLFFYYYNKNDKIRVTDLHQGVVWGTNTEDTKQDERLINRFDYD
GDYGTVLNRFLMQAAIGHPLTVHGTGGQTRAFIHIQDTVKCIQLAVENPPEAGERVKIFNQMTETHRVRDLAKIIADITG
AKIDYLENPRTEAAENELFVENKCFMDLGLQPTKLDVGLMKEVTEIAAKYKDRCDHSKILCTSKWRK
>Mature_387_residues
MNIVILGGDGFCGWPTALHLSKLGHDVVIVDNLSRRNIDNELETNSLTPISPISIRLKTWQDITGKHIQFYNFDIAAEYD
RLLNLFCTYRPDVVVHFAEQRAAPYSMKSSRQKRYTVNNNLNATNNVLAAIVESGLDIHVVHLGTMGVYGYGTAGMKIPE
GYLDVQVVIDTGEVVEKQILYPADPGSVYHMTKTQDQLFFYYYNKNDKIRVTDLHQGVVWGTNTEDTKQDERLINRFDYD
GDYGTVLNRFLMQAAIGHPLTVHGTGGQTRAFIHIQDTVKCIQLAVENPPEAGERVKIFNQMTETHRVRDLAKIIADITG
AKIDYLENPRTEAAENELFVENKCFMDLGLQPTKLDVGLMKEVTEIAAKYKDRCDHSKILCTSKWRK

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 43834; Mature: 43834

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVILGGDGFCGWPTALHLSKLGHDVVIVDNLSRRNIDNELETNSLTPISPISIRLKTW
CEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEC
QDITGKHIQFYNFDIAAEYDRLLNLFCTYRPDVVVHFAEQRAAPYSMKSSRQKRYTVNNN
CCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHCCCCCEEEECCC
LNATNNVLAAIVESGLDIHVVHLGTMGVYGYGTAGMKIPEGYLDVQVVIDTGEVVEKQIL
CCCHHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCCCCCCEEEEEEEECCHHHHHHEE
YPADPGSVYHMTKTQDQLFFYYYNKNDKIRVTDLHQGVVWGTNTEDTKQDERLINRFDYD
CCCCCCCEEEEECCCCEEEEEEECCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCC
GDYGTVLNRFLMQAAIGHPLTVHGTGGQTRAFIHIQDTVKCIQLAVENPPEAGERVKIFN
CCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHH
QMTETHRVRDLAKIIADITGAKIDYLENPRTEAAENELFVENKCFMDLGLQPTKLDVGLM
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCEEEECCCCCCEECHHHH
KEVTEIAAKYKDRCDHSKILCTSKWRK
HHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MNIVILGGDGFCGWPTALHLSKLGHDVVIVDNLSRRNIDNELETNSLTPISPISIRLKTW
CEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEC
QDITGKHIQFYNFDIAAEYDRLLNLFCTYRPDVVVHFAEQRAAPYSMKSSRQKRYTVNNN
CCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHCCCCCEEEECCC
LNATNNVLAAIVESGLDIHVVHLGTMGVYGYGTAGMKIPEGYLDVQVVIDTGEVVEKQIL
CCCHHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCCCCCCEEEEEEEECCHHHHHHEE
YPADPGSVYHMTKTQDQLFFYYYNKNDKIRVTDLHQGVVWGTNTEDTKQDERLINRFDYD
CCCCCCCEEEEECCCCEEEEEEECCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCC
GDYGTVLNRFLMQAAIGHPLTVHGTGGQTRAFIHIQDTVKCIQLAVENPPEAGERVKIFN
CCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHH
QMTETHRVRDLAKIIADITGAKIDYLENPRTEAAENELFVENKCFMDLGLQPTKLDVGLM
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCEEEECCCCCCEECHHHH
KEVTEIAAKYKDRCDHSKILCTSKWRK
HHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA