Click here to switch to the map view.

The map label for this gene is ubiE [H]

Identifier: 158335894

GI number: 158335894

Start: 2788985

End: 2789839

Strand: Reverse

Name: ubiE [H]

Synonym: AM1_2752

Alternate gene names: 158335894

Gene position: 2789839-2788985 (Counterclockwise)

Preceding gene: 158335901

Following gene: 158335891

Centisome position: 42.9

GC content: 49.47

Gene sequence:

>855_bases
GTGAAACCACTTGCATTAGATAAATTTAAACAAGGGGTTGCAGACTTTTATGATCGTAGGAGTCGAAGTTATGACCAGGG
GCAGTGGCACCCTCAAATTTGTGACCGCCTGCTGGAATATGCGCGGATTGGTGCTGGTCAGACGGTTTTAGATATTGGTA
CAGGAACCGGCTACCTTGCGATCGCATCTGCCCAACGAGTTGGCGATCAAGGACATGTGACTGGCGTAGATATTTCTCCA
GGTATGCTGCAGCAAGCACAGCGCAAAATTCAGCGGTTAGGACTGAGCAACGTTGTGGTTCAACGGGCTGATGCTGAAGC
CCTGGACTATCCCAGCCATCATTTTGACGTTATCCTTTGTGCCCATACGTTTCCTTGGATGACGGATAAAGCCGCGACAT
TGCGTTTGTGGTATCAGTTACTCAAGCCCAGGGGGCGCATCGCCGTTCATACCCCTGCTGACACTGCCTATATTGGGGCT
GTGGTGTTACGCAGAGTGCTGGCTGAGTATGGTCTTGAACTAGAGCCTAGCAATCGGTTAGGTTCGATTGAGCAATGTCG
GCAGTTGTTTGAAAAGGCTGGTTTTGAGGCGGTTGAGATTTATACCGAGCAACATGGCAGCTACACAACTCTGGATCAAG
CCAAAGCCATTTGGGAGAGCGTTGTGGTGAATCCTTCTATTACTGCTCCAAAGGTTGTTGGTGATGGGCGATCCCGGTTT
TCCGCAACAGAGTTAGGAAAAATCAAAGCTGAACTATATGCAGAACTGGAAGCCCTGCAAACGGAGCAGGGCCTCTGGAA
TGATCTAACGACGGTATATATTTTGGCCGATAAGCCAGAGCCGTATTGTGGTTGA

Upstream 100 bases:

>100_bases
CAAAGTGAGATGTTGCAGCGCACCGCTTTTGATTCGCCAAGTGCTGCTGAGGATTCGACTTCCCGTTAGGCTAGAGGGCA
GTTGTAGATCATGACTGAAT

Downstream 100 bases:

>100_bases
GTCTATCACCATCCATTAGCATTGGCTGAATGTTCAAATCGTGCTCACATCAATATGACCCTATTCATCAGTAATGATAT
TTGGCTTGGATAAAGTCAAT

Product: methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKPLALDKFKQGVADFYDRRSRSYDQGQWHPQICDRLLEYARIGAGQTVLDIGTGTGYLAIASAQRVGDQGHVTGVDISP
GMLQQAQRKIQRLGLSNVVVQRADAEALDYPSHHFDVILCAHTFPWMTDKAATLRLWYQLLKPRGRIAVHTPADTAYIGA
VVLRRVLAEYGLELEPSNRLGSIEQCRQLFEKAGFEAVEIYTEQHGSYTTLDQAKAIWESVVVNPSITAPKVVGDGRSRF
SATELGKIKAELYAELEALQTEQGLWNDLTTVYILADKPEPYCG

Sequences:

>Translated_284_residues
MKPLALDKFKQGVADFYDRRSRSYDQGQWHPQICDRLLEYARIGAGQTVLDIGTGTGYLAIASAQRVGDQGHVTGVDISP
GMLQQAQRKIQRLGLSNVVVQRADAEALDYPSHHFDVILCAHTFPWMTDKAATLRLWYQLLKPRGRIAVHTPADTAYIGA
VVLRRVLAEYGLELEPSNRLGSIEQCRQLFEKAGFEAVEIYTEQHGSYTTLDQAKAIWESVVVNPSITAPKVVGDGRSRF
SATELGKIKAELYAELEALQTEQGLWNDLTTVYILADKPEPYCG
>Mature_284_residues
MKPLALDKFKQGVADFYDRRSRSYDQGQWHPQICDRLLEYARIGAGQTVLDIGTGTGYLAIASAQRVGDQGHVTGVDISP
GMLQQAQRKIQRLGLSNVVVQRADAEALDYPSHHFDVILCAHTFPWMTDKAATLRLWYQLLKPRGRIAVHTPADTAYIGA
VVLRRVLAEYGLELEPSNRLGSIEQCRQLFEKAGFEAVEIYTEQHGSYTTLDQAKAIWESVVVNPSITAPKVVGDGRSRF
SATELGKIKAELYAELEALQTEQGLWNDLTTVYILADKPEPYCG

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=184, Percent_Identity=33.1521739130435, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31610; Mature: 31610

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLALDKFKQGVADFYDRRSRSYDQGQWHPQICDRLLEYARIGAGQTVLDIGTGTGYLA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
IASAQRVGDQGHVTGVDISPGMLQQAQRKIQRLGLSNVVVQRADAEALDYPSHHFDVILC
EEHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCCEEEEEE
AHTFPWMTDKAATLRLWYQLLKPRGRIAVHTPADTAYIGAVVLRRVLAEYGLELEPSNRL
ECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEECCCCCC
GSIEQCRQLFEKAGFEAVEIYTEQHGSYTTLDQAKAIWESVVVNPSITAPKVVGDGRSRF
CCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCHHC
SATELGKIKAELYAELEALQTEQGLWNDLTTVYILADKPEPYCG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKPLALDKFKQGVADFYDRRSRSYDQGQWHPQICDRLLEYARIGAGQTVLDIGTGTGYLA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
IASAQRVGDQGHVTGVDISPGMLQQAQRKIQRLGLSNVVVQRADAEALDYPSHHFDVILC
EEHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCCEEEEEE
AHTFPWMTDKAATLRLWYQLLKPRGRIAVHTPADTAYIGAVVLRRVLAEYGLELEPSNRL
ECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEECCCCCC
GSIEQCRQLFEKAGFEAVEIYTEQHGSYTTLDQAKAIWESVVVNPSITAPKVVGDGRSRF
CCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCHHC
SATELGKIKAELYAELEALQTEQGLWNDLTTVYILADKPEPYCG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA