Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 158335716

GI number: 158335716

Start: 2604291

End: 2605799

Strand: Direct

Name: fadH [C]

Synonym: AM1_2566

Alternate gene names: 158335716

Gene position: 2604291-2605799 (Clockwise)

Preceding gene: 158335715

Following gene: 158335717

Centisome position: 40.04

GC content: 52.15

Gene sequence:

>1509_bases
ATGACCTCAGACATTATCTTTCAGCCCTTAGAGTGGCGCAATCTAACGGTTAAAAATCGCATTTTCCGATCCAGCATCTC
AGGGCGTTGGGATAACTACGATGGTTCGGGCACCCAAGCCCGGATCAATTGGGAAGAGCAGTTTGCCCAGGGTGGCGTCG
GTGCCATTATCTCGTCCTATACCCCCATTCATATTGAAGGACGGATTGTGCCGAATGCTGCCATGATTGACTCGGACGAC
CGCATTCCCTTCTGGAAAAAGGTTGGCGAGACGGTTCATCAGCACGACTGCAAGTTTATCCTCCAGCTCAGCCATTCGGG
GCGTCAGCGCGATATTGATGGGGTCGAAAATACCGTTCCCGATTCCTTTAAGCCCACACCTGCCCTGAGTGCAACGACCC
TCTCAGAACAGATTCATGGGTTCCGCTGTCGCCAGATGACCCAGACAGAAATCCAGCAGACGGTACAAAGGTTTGCGGAT
GGGGCGCACCGGGCTCGGGAAGCTGGACTAGATGGCGTGGAATTACATAGCTCCCATGGCTATCTGATTACCCAATTCCT
CAGTTCTGGCATCAATATTCGCAATGATGACTATGGGGGGTCTCTCGAAAATCGATATCGGTTCCTGCAGGAAATTGTCC
AGGCTATTCGCCAAACTGTCGGTGATGATTTTCATCTCCAGGCCAAGATTAGTGCCGTGGACTATAACAATGTCTTGCCC
TGGGAACGGAAGGGCAACACCCTATCTGAGTCCATTCAAGTCTGTCAGTGGCTCGAACGAGATGGGGTAGATGCTCTCCA
TATTTCTCGGGGCAGTACCTTTCCCCATCCCCTATTACCCGCAGGGCCTTTTCCCTTTAAGATTGCCACCTCCACCTACG
ACACGATGATTTCCAGTGCCCCGTGGCGCACCCTCCTCAATTACTTTCTATTTCGCAGTCCCTTACTTCAGCCCTTTTTT
AAGTGCATCTGGAATCGCTTGGAGCGGCATTGGCAGCCTCCCGTTAAAGGCGATCGTCTCTCGTCAGATTCTCTCTCCCA
CTTGATGCAAGAGCATATCTCTACCCATGAGTTTCAGGCCCTATTAGCCCGCTACCAAGGCACGGTTATGGAAGATGCCC
AGGCCATTAAGGAACAGGTCAATATTCCTGTTATTTGCACCGGCGGCTTCCAGCAAGCCTCTTTTATCCGGCAAGCGATT
GAGAAGGGATTCTGTGATGGTGTTACCCTTGCCCGCCCGCTGGTCGCCAATAAGGATCTGGCCCACCAGTTTCAGGCAGG
AAACGATATTCCCGAGCGACCTTGTACCTATTGCAATCAATGCTTGGGGAGTTATCTGGAGTATCCCCTGGGCTGCTATG
AACGCCGTCGATATTACCAGCGCTCTATTACTGGCGCAGATAAAGCGGAGCAAGATAAAGCGGCACGAGAAGCCCATGAC
GCCATGATTCAAGATGCTATGCGGGTTTATAGCCCACCGCCCTTTAGCCCGGCCGAGCAAACGGCATAA

Upstream 100 bases:

>100_bases
CTCCGAGTTTGTGGCTTCCTTCTACGGCGACAGTCAGCTCTTCTTTCGGCATCAGCGCTTCCGTAACCAGTAGTCCGTCA
CTCTGCCATAAGGAAATCCT

Downstream 100 bases:

>100_bases
CCTCGGATGCAAATTGCAAGCGTGATATCCCAATGAGCAGTACACCCTTTTTCCAGAAAGTCTGTCGTAACTTCAAGCGG
TTAGCCCTAGGGCTGGTTAG

Product: NADH-flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTSDIIFQPLEWRNLTVKNRIFRSSISGRWDNYDGSGTQARINWEEQFAQGGVGAIISSYTPIHIEGRIVPNAAMIDSDD
RIPFWKKVGETVHQHDCKFILQLSHSGRQRDIDGVENTVPDSFKPTPALSATTLSEQIHGFRCRQMTQTEIQQTVQRFAD
GAHRAREAGLDGVELHSSHGYLITQFLSSGINIRNDDYGGSLENRYRFLQEIVQAIRQTVGDDFHLQAKISAVDYNNVLP
WERKGNTLSESIQVCQWLERDGVDALHISRGSTFPHPLLPAGPFPFKIATSTYDTMISSAPWRTLLNYFLFRSPLLQPFF
KCIWNRLERHWQPPVKGDRLSSDSLSHLMQEHISTHEFQALLARYQGTVMEDAQAIKEQVNIPVICTGGFQQASFIRQAI
EKGFCDGVTLARPLVANKDLAHQFQAGNDIPERPCTYCNQCLGSYLEYPLGCYERRRYYQRSITGADKAEQDKAAREAHD
AMIQDAMRVYSPPPFSPAEQTA

Sequences:

>Translated_502_residues
MTSDIIFQPLEWRNLTVKNRIFRSSISGRWDNYDGSGTQARINWEEQFAQGGVGAIISSYTPIHIEGRIVPNAAMIDSDD
RIPFWKKVGETVHQHDCKFILQLSHSGRQRDIDGVENTVPDSFKPTPALSATTLSEQIHGFRCRQMTQTEIQQTVQRFAD
GAHRAREAGLDGVELHSSHGYLITQFLSSGINIRNDDYGGSLENRYRFLQEIVQAIRQTVGDDFHLQAKISAVDYNNVLP
WERKGNTLSESIQVCQWLERDGVDALHISRGSTFPHPLLPAGPFPFKIATSTYDTMISSAPWRTLLNYFLFRSPLLQPFF
KCIWNRLERHWQPPVKGDRLSSDSLSHLMQEHISTHEFQALLARYQGTVMEDAQAIKEQVNIPVICTGGFQQASFIRQAI
EKGFCDGVTLARPLVANKDLAHQFQAGNDIPERPCTYCNQCLGSYLEYPLGCYERRRYYQRSITGADKAEQDKAAREAHD
AMIQDAMRVYSPPPFSPAEQTA
>Mature_501_residues
TSDIIFQPLEWRNLTVKNRIFRSSISGRWDNYDGSGTQARINWEEQFAQGGVGAIISSYTPIHIEGRIVPNAAMIDSDDR
IPFWKKVGETVHQHDCKFILQLSHSGRQRDIDGVENTVPDSFKPTPALSATTLSEQIHGFRCRQMTQTEIQQTVQRFADG
AHRAREAGLDGVELHSSHGYLITQFLSSGINIRNDDYGGSLENRYRFLQEIVQAIRQTVGDDFHLQAKISAVDYNNVLPW
ERKGNTLSESIQVCQWLERDGVDALHISRGSTFPHPLLPAGPFPFKIATSTYDTMISSAPWRTLLNYFLFRSPLLQPFFK
CIWNRLERHWQPPVKGDRLSSDSLSHLMQEHISTHEFQALLARYQGTVMEDAQAIKEQVNIPVICTGGFQQASFIRQAIE
KGFCDGVTLARPLVANKDLAHQFQAGNDIPERPCTYCNQCLGSYLEYPLGCYERRRYYQRSITGADKAEQDKAAREAHDA
MIQDAMRVYSPPPFSPAEQTA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=291, Percent_Identity=31.9587628865979, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1787939, Length=281, Percent_Identity=31.3167259786477, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=276, Percent_Identity=30.0724637681159, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=291, Percent_Identity=29.553264604811, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17540738, Length=291, Percent_Identity=29.2096219931272, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17559804, Length=299, Percent_Identity=28.7625418060201, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17564188, Length=292, Percent_Identity=28.4246575342466, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=289, Percent_Identity=28.719723183391, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=288, Percent_Identity=26.0416666666667, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=162, Percent_Identity=32.0987654320988, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=172, Percent_Identity=33.1395348837209, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 56944; Mature: 56812

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDIIFQPLEWRNLTVKNRIFRSSISGRWDNYDGSGTQARINWEEQFAQGGVGAIISSY
CCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHCCCHHHHHHCC
TPIHIEGRIVPNAAMIDSDDRIPFWKKVGETVHQHDCKFILQLSHSGRQRDIDGVENTVP
CCEEEECEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
DSFKPTPALSATTLSEQIHGFRCRQMTQTEIQQTVQRFADGAHRAREAGLDGVELHSSHG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLITQFLSSGINIRNDDYGGSLENRYRFLQEIVQAIRQTVGDDFHLQAKISAVDYNNVLP
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
WERKGNTLSESIQVCQWLERDGVDALHISRGSTFPHPLLPAGPFPFKIATSTYDTMISSA
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHCCC
PWRTLLNYFLFRSPLLQPFFKCIWNRLERHWQPPVKGDRLSSDSLSHLMQEHISTHEFQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLARYQGTVMEDAQAIKEQVNIPVICTGGFQQASFIRQAIEKGFCDGVTLARPLVANKDL
HHHHHCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
AHQFQAGNDIPERPCTYCNQCLGSYLEYPLGCYERRRYYQRSITGADKAEQDKAAREAHD
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AMIQDAMRVYSPPPFSPAEQTA
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TSDIIFQPLEWRNLTVKNRIFRSSISGRWDNYDGSGTQARINWEEQFAQGGVGAIISSY
CCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHCCCHHHHHHCC
TPIHIEGRIVPNAAMIDSDDRIPFWKKVGETVHQHDCKFILQLSHSGRQRDIDGVENTVP
CCEEEECEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
DSFKPTPALSATTLSEQIHGFRCRQMTQTEIQQTVQRFADGAHRAREAGLDGVELHSSHG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLITQFLSSGINIRNDDYGGSLENRYRFLQEIVQAIRQTVGDDFHLQAKISAVDYNNVLP
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
WERKGNTLSESIQVCQWLERDGVDALHISRGSTFPHPLLPAGPFPFKIATSTYDTMISSA
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHCCC
PWRTLLNYFLFRSPLLQPFFKCIWNRLERHWQPPVKGDRLSSDSLSHLMQEHISTHEFQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLARYQGTVMEDAQAIKEQVNIPVICTGGFQQASFIRQAIEKGFCDGVTLARPLVANKDL
HHHHHCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
AHQFQAGNDIPERPCTYCNQCLGSYLEYPLGCYERRRYYQRSITGADKAEQDKAAREAHD
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AMIQDAMRVYSPPPFSPAEQTA
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]