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The map label for this gene is dnaG [H]

Identifier: 158335712

GI number: 158335712

Start: 2597313

End: 2599367

Strand: Reverse

Name: dnaG [H]

Synonym: AM1_2562

Alternate gene names: 158335712

Gene position: 2599367-2597313 (Counterclockwise)

Preceding gene: 158335720

Following gene: 158335711

Centisome position: 39.97

GC content: 52.7

Gene sequence:

>2055_bases
ATGACTACGCCCCGCCTCCACCCCGATACGATTGAGCAGGTTCGCCAAGCTACCGACATTGTCGATGTGGTTTCCGAGCA
TGTCAGCTTGCGCAAGCAGGGGCGGAACTTAGTGGGCTGCTGCCCATTTCACGACGAGAAAACCCCCAGCTTTAGTGTTA
GCCCCGAGAAACAGTTCTATTACTGCTTTGGCTGTGGAGCTGGTGGCAATGCCATCAAGTTTTTGATGGAGATTGGCCAG
CGGTCCTTTAGTGAAGTCGTCCTCGATTTGGCCCAGCGCAGTCAAGTTCCTATCCAAACCCTCGCTAAAGACGAAGAGAA
AAAGCTAGCCCGTAAGCTGTCCCTGCGAGAGCAGCTCTATGAGATTTTGGCGATCGCAAGTCGGTATTACGAACATGCCC
TCCGCCAGCCCCAGGGCCAAAATGCCCTCCGCTATGCGCGTAACCAGCGGGGACTCAGCAATGAAACCATTCAAACCTTT
CAGCTGGGCTATGCTCCGGGAGGCTGGAGTACCCTCCATGATGTGTTGGTTCAGGAGAAGGGATTTAGCCCAGAATTGGT
CCAGCAGGCAGGATTGATTGTGCCCCGCAAGTCTGGGGATGGATATTACGATCGGTTCCGCGATCGCTTAATGATACCGA
TCCACGATGCCCAAAGCCGGGTGGTGGGATTTGGCAGCCGCACCCTCACAGGAGAGGAACCCAAATATCTCAACTCCCCA
GAAACAGAACTCTTCAATAAAGGCAAACTCCTATTTGGTCTAGATAAAGCTCGGCAAACCATCGTCAAGGCCGACCGGGC
GGTGGTGGTCGAAGGCTATTTTGACGTGATTGCCCTGCATTCTGTAGGCATCACTAATGCCGTTGCCTCTATGGGAACAG
CCCTAAGCTTGGACCAGATGCGGCAGTTGCTGCGTTACACCGAATCCAAACAGGTCATTCTCAATTTCGATGCGGATGCA
GCAGGCGGTAAAGCCGCAGAACGAGCGATTTCTGAAGTTGAAGACCAGGCCTATCGGGGTGAAGTTCAGCTCCGAGTCTT
GAATTTGCCCAATGGCAAAGATGCGGATGAGTATCTGCAGGAGTATACGGCAGAAGACTATCTGGATTTGCTCGATGAGT
CCCCCCTGTGGCTAGACTGGCAAATTCACCGGGCTCTGCAAGATCGGGATTTGCAGCAGGCCGAACAGTTCCAAGCAGCC
ACGGAAGCCATTGCGGCTATTTTAGGAAAGCTGCCCAACGCCACCCTGCGCACCCACTATACCCATCGCTGTGCAGGCCT
CCTCAGTCAGGGGGATTCTCGCTTGGCCCTGCAGCTGGAAGAAGCGCTGCGCCAGCAGGTTCGCGGCCAACGCTGGCATG
GGCGATCGCAAAAATGGCAGCGCCCGGCCGACTACACCCTGCGGGAAGCAGCGGAAGCCCAAATCCTTCGTATTTATCTG
CATCGACCCCAGTTTCGGCAAGATATTCACAACGCCATGAAACAGCGGGATATAGAATTCAGTTTTTCCCACCATCGGTT
TCTATGGCGGCAAATTTTAGCCATTGAAGATGATCAGGCCAAGTTAGAGCCCTTACATCCTGAACTGGAAGCCCCTGCCT
TTGATCTAATCGCTGTGGTCCAGGATCTTTGTACCGAGTATTCGCAGGAAATGCAGCAGGTTTACCACCTGATTCAGCTG
AACGAAAAAACAGAACTGGATATCCTCCGCCCCAACCTCTCCATTCGGGCAGCAGCGGCTAGTCTAGAGCGGATCGCGTG
TGAGAAGCGCTGTCGGCATCTGCTGCAAATGTGGGAATCGGCGATGAAATCCGCAAAAAAAGAACTGGGTCAGCGGCAAA
TCCTCAACACTTATCTACAACAGATGTTGATGGATGAATCGGAGCAGGCCCAGAGTTTACTAGAGGAAGACTCCTATTGT
CTGCAAGAGCTGGATGAAATTAAACGGCTCTATTACCAAGAAAAGCGCTACTTGCAACAGCTGGATCAGCAGCGCTGTGT
GGATCTCAATGATTTCAGTGAATTGATGGAAAATCCCCAGCTCAATTTGAACTGA

Upstream 100 bases:

>100_bases
AGAGATATCGTCCCTATTGGTGTTCAGCTTTTAAAATCCTCACTGCCTTAGCCTCCCGCTTCTCCCACGGAATGGGTGTC
AAATTGCCCTCTCAACCTGG

Downstream 100 bases:

>100_bases
AGAGGAGAGATTTGAAGTCTGGTGTAGCTCTACATCTTAGGGTTACACCTAAACCTGAAATGAATAACGACATCCCTGCC
AATGCCTAAATTCTGGGTCT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MTTPRLHPDTIEQVRQATDIVDVVSEHVSLRKQGRNLVGCCPFHDEKTPSFSVSPEKQFYYCFGCGAGGNAIKFLMEIGQ
RSFSEVVLDLAQRSQVPIQTLAKDEEKKLARKLSLREQLYEILAIASRYYEHALRQPQGQNALRYARNQRGLSNETIQTF
QLGYAPGGWSTLHDVLVQEKGFSPELVQQAGLIVPRKSGDGYYDRFRDRLMIPIHDAQSRVVGFGSRTLTGEEPKYLNSP
ETELFNKGKLLFGLDKARQTIVKADRAVVVEGYFDVIALHSVGITNAVASMGTALSLDQMRQLLRYTESKQVILNFDADA
AGGKAAERAISEVEDQAYRGEVQLRVLNLPNGKDADEYLQEYTAEDYLDLLDESPLWLDWQIHRALQDRDLQQAEQFQAA
TEAIAAILGKLPNATLRTHYTHRCAGLLSQGDSRLALQLEEALRQQVRGQRWHGRSQKWQRPADYTLREAAEAQILRIYL
HRPQFRQDIHNAMKQRDIEFSFSHHRFLWRQILAIEDDQAKLEPLHPELEAPAFDLIAVVQDLCTEYSQEMQQVYHLIQL
NEKTELDILRPNLSIRAAAASLERIACEKRCRHLLQMWESAMKSAKKELGQRQILNTYLQQMLMDESEQAQSLLEEDSYC
LQELDEIKRLYYQEKRYLQQLDQQRCVDLNDFSELMENPQLNLN

Sequences:

>Translated_684_residues
MTTPRLHPDTIEQVRQATDIVDVVSEHVSLRKQGRNLVGCCPFHDEKTPSFSVSPEKQFYYCFGCGAGGNAIKFLMEIGQ
RSFSEVVLDLAQRSQVPIQTLAKDEEKKLARKLSLREQLYEILAIASRYYEHALRQPQGQNALRYARNQRGLSNETIQTF
QLGYAPGGWSTLHDVLVQEKGFSPELVQQAGLIVPRKSGDGYYDRFRDRLMIPIHDAQSRVVGFGSRTLTGEEPKYLNSP
ETELFNKGKLLFGLDKARQTIVKADRAVVVEGYFDVIALHSVGITNAVASMGTALSLDQMRQLLRYTESKQVILNFDADA
AGGKAAERAISEVEDQAYRGEVQLRVLNLPNGKDADEYLQEYTAEDYLDLLDESPLWLDWQIHRALQDRDLQQAEQFQAA
TEAIAAILGKLPNATLRTHYTHRCAGLLSQGDSRLALQLEEALRQQVRGQRWHGRSQKWQRPADYTLREAAEAQILRIYL
HRPQFRQDIHNAMKQRDIEFSFSHHRFLWRQILAIEDDQAKLEPLHPELEAPAFDLIAVVQDLCTEYSQEMQQVYHLIQL
NEKTELDILRPNLSIRAAAASLERIACEKRCRHLLQMWESAMKSAKKELGQRQILNTYLQQMLMDESEQAQSLLEEDSYC
LQELDEIKRLYYQEKRYLQQLDQQRCVDLNDFSELMENPQLNLN
>Mature_683_residues
TTPRLHPDTIEQVRQATDIVDVVSEHVSLRKQGRNLVGCCPFHDEKTPSFSVSPEKQFYYCFGCGAGGNAIKFLMEIGQR
SFSEVVLDLAQRSQVPIQTLAKDEEKKLARKLSLREQLYEILAIASRYYEHALRQPQGQNALRYARNQRGLSNETIQTFQ
LGYAPGGWSTLHDVLVQEKGFSPELVQQAGLIVPRKSGDGYYDRFRDRLMIPIHDAQSRVVGFGSRTLTGEEPKYLNSPE
TELFNKGKLLFGLDKARQTIVKADRAVVVEGYFDVIALHSVGITNAVASMGTALSLDQMRQLLRYTESKQVILNFDADAA
GGKAAERAISEVEDQAYRGEVQLRVLNLPNGKDADEYLQEYTAEDYLDLLDESPLWLDWQIHRALQDRDLQQAEQFQAAT
EAIAAILGKLPNATLRTHYTHRCAGLLSQGDSRLALQLEEALRQQVRGQRWHGRSQKWQRPADYTLREAAEAQILRIYLH
RPQFRQDIHNAMKQRDIEFSFSHHRFLWRQILAIEDDQAKLEPLHPELEAPAFDLIAVVQDLCTEYSQEMQQVYHLIQLN
EKTELDILRPNLSIRAAAASLERIACEKRCRHLLQMWESAMKSAKKELGQRQILNTYLQQMLMDESEQAQSLLEEDSYCL
QELDEIKRLYYQEKRYLQQLDQQRCVDLNDFSELMENPQLNLN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=405, Percent_Identity=39.5061728395062, Blast_Score=284, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 78642; Mature: 78511

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPRLHPDTIEQVRQATDIVDVVSEHVSLRKQGRNLVGCCPFHDEKTPSFSVSPEKQFY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEE
YCFGCGAGGNAIKFLMEIGQRSFSEVVLDLAQRSQVPIQTLAKDEEKKLARKLSLREQLY
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHH
EILAIASRYYEHALRQPQGQNALRYARNQRGLSNETIQTFQLGYAPGGWSTLHDVLVQEK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHEECCCCCCHHHHHHHHHHCC
GFSPELVQQAGLIVPRKSGDGYYDRFRDRLMIPIHDAQSRVVGFGSRTLTGEEPKYLNSP
CCCHHHHHHCCCEEECCCCCCHHHHHHCCEEEEEECCCCHHEECCCCCCCCCCCCCCCCC
ETELFNKGKLLFGLDKARQTIVKADRAVVVEGYFDVIALHSVGITNAVASMGTALSLDQM
HHHHHCCCCEEEEHHHHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHCCCCCHHHH
RQLLRYTESKQVILNFDADAAGGKAAERAISEVEDQAYRGEVQLRVLNLPNGKDADEYLQ
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
EYTAEDYLDLLDESPLWLDWQIHRALQDRDLQQAEQFQAATEAIAAILGKLPNATLRTHY
HHHHHHHHHHHCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
THRCAGLLSQGDSRLALQLEEALRQQVRGQRWHGRSQKWQRPADYTLREAAEAQILRIYL
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HRPQFRQDIHNAMKQRDIEFSFSHHRFLWRQILAIEDDQAKLEPLHPELEAPAFDLIAVV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
QDLCTEYSQEMQQVYHLIQLNEKTELDILRPNLSIRAAAASLERIACEKRCRHLLQMWES
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AMKSAKKELGQRQILNTYLQQMLMDESEQAQSLLEEDSYCLQELDEIKRLYYQEKRYLQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQQRCVDLNDFSELMENPQLNLN
HHHHHCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTPRLHPDTIEQVRQATDIVDVVSEHVSLRKQGRNLVGCCPFHDEKTPSFSVSPEKQFY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEE
YCFGCGAGGNAIKFLMEIGQRSFSEVVLDLAQRSQVPIQTLAKDEEKKLARKLSLREQLY
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHH
EILAIASRYYEHALRQPQGQNALRYARNQRGLSNETIQTFQLGYAPGGWSTLHDVLVQEK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHEECCCCCCHHHHHHHHHHCC
GFSPELVQQAGLIVPRKSGDGYYDRFRDRLMIPIHDAQSRVVGFGSRTLTGEEPKYLNSP
CCCHHHHHHCCCEEECCCCCCHHHHHHCCEEEEEECCCCHHEECCCCCCCCCCCCCCCCC
ETELFNKGKLLFGLDKARQTIVKADRAVVVEGYFDVIALHSVGITNAVASMGTALSLDQM
HHHHHCCCCEEEEHHHHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHCCCCCHHHH
RQLLRYTESKQVILNFDADAAGGKAAERAISEVEDQAYRGEVQLRVLNLPNGKDADEYLQ
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
EYTAEDYLDLLDESPLWLDWQIHRALQDRDLQQAEQFQAATEAIAAILGKLPNATLRTHY
HHHHHHHHHHHCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
THRCAGLLSQGDSRLALQLEEALRQQVRGQRWHGRSQKWQRPADYTLREAAEAQILRIYL
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HRPQFRQDIHNAMKQRDIEFSFSHHRFLWRQILAIEDDQAKLEPLHPELEAPAFDLIAVV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
QDLCTEYSQEMQQVYHLIQLNEKTELDILRPNLSIRAAAASLERIACEKRCRHLLQMWES
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AMKSAKKELGQRQILNTYLQQMLMDESEQAQSLLEEDSYCLQELDEIKRLYYQEKRYLQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQQRCVDLNDFSELMENPQLNLN
HHHHHCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA