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The map label for this gene is hisI [H]

Identifier: 158335708

GI number: 158335708

Start: 2594350

End: 2594979

Strand: Reverse

Name: hisI [H]

Synonym: AM1_2558

Alternate gene names: 158335708

Gene position: 2594979-2594350 (Counterclockwise)

Preceding gene: 158335711

Following gene: 158335707

Centisome position: 39.9

GC content: 50.32

Gene sequence:

>630_bases
ATGTCTTCTTCTGCAATTCCAGTAGACAAAATTCGGTATGACGTTCAAGGGCTCGTACCAGCCATTGTTCAAGACTATTT
AGATGGCACGGTGTTGATGATGGCCTGGATGAATCGTGAATCTTTGCAGAAGACCATGGATACGGGCCGGACTTGGTTTT
GGAGCCGATCGCGTCAGGAGCTGTGGCCAAAAGGTGAAACCTCGGGTCATGTGCAAGAGGTGAAGTCCCTGCGGTACGAC
TGTGACAGTGATGCCTTACTGGTTGGGGTGGAGCAAATTGGTGATATTGCCTGCCATACGGGAGAACGTAGCTGTTTCCA
TCAGATTGAAGGGCAGATTGCGGCTCCCCCTGCTGATACTTTGTCTCAGGTGTTTGATGTGATTTGCGATCGCAAACAGA
ATCCCTCCCCGGACTCCTATACCTGCAAGCTATTTGCTGCGGGTGATAACAAAATTCTCAAAAAGATTGGTGAAGAATCT
GCAGAAGTGGTGATGGCCTGTAAGGACGACAATGCGGATGAAATTGCGGGAGAAGTGGCCGATCTGATTTACCACAGTCT
CGTGGCCCTGGCCCACCATGATGTAGATATCAAGGACGTCTACCGCAAACTGCAAGAGCGGCGACGCTAG

Upstream 100 bases:

>100_bases
TATTAGTATTTCTTATTAAAAAAGCCAATCTCAATTAATATATCCGTATTGTTCGGTCCCAGGTGATAATCTAAAGAGTT
GTTTTCTGCTGCTCACATTG

Downstream 100 bases:

>100_bases
GCATGAAGCGCGAAGTTTGGATCGTCTCTGCCATTGCCTTAGTCAACTCCCTTAGTTTTACGGTTTTGCTGCCGACCCTG
TATCCCTACGGTCGGCAGTT

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSSSAIPVDKIRYDVQGLVPAIVQDYLDGTVLMMAWMNRESLQKTMDTGRTWFWSRSRQELWPKGETSGHVQEVKSLRYD
CDSDALLVGVEQIGDIACHTGERSCFHQIEGQIAAPPADTLSQVFDVICDRKQNPSPDSYTCKLFAAGDNKILKKIGEES
AEVVMACKDDNADEIAGEVADLIYHSLVALAHHDVDIKDVYRKLQERRR

Sequences:

>Translated_209_residues
MSSSAIPVDKIRYDVQGLVPAIVQDYLDGTVLMMAWMNRESLQKTMDTGRTWFWSRSRQELWPKGETSGHVQEVKSLRYD
CDSDALLVGVEQIGDIACHTGERSCFHQIEGQIAAPPADTLSQVFDVICDRKQNPSPDSYTCKLFAAGDNKILKKIGEES
AEVVMACKDDNADEIAGEVADLIYHSLVALAHHDVDIKDVYRKLQERRR
>Mature_208_residues
SSSAIPVDKIRYDVQGLVPAIVQDYLDGTVLMMAWMNRESLQKTMDTGRTWFWSRSRQELWPKGETSGHVQEVKSLRYDC
DSDALLVGVEQIGDIACHTGERSCFHQIEGQIAAPPADTLSQVFDVICDRKQNPSPDSYTCKLFAAGDNKILKKIGEESA
EVVMACKDDNADEIAGEVADLIYHSLVALAHHDVDIKDVYRKLQERRR

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=193, Percent_Identity=40.4145077720207, Blast_Score=147, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6319819, Length=192, Percent_Identity=35.4166666666667, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH [H]

EC number: =3.5.4.19; =3.6.1.31 [H]

Molecular weight: Translated: 23475; Mature: 23344

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSAIPVDKIRYDVQGLVPAIVQDYLDGTVLMMAWMNRESLQKTMDTGRTWFWSRSRQE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHCCCCEEECCCHHH
LWPKGETSGHVQEVKSLRYDCDSDALLVGVEQIGDIACHTGERSCFHQIEGQIAAPPADT
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHH
LSQVFDVICDRKQNPSPDSYTCKLFAAGDNKILKKIGEESAEVVMACKDDNADEIAGEVA
HHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHCCCHHHEEEEECCCCHHHHHHHHH
DLIYHSLVALAHHDVDIKDVYRKLQERRR
HHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SSSAIPVDKIRYDVQGLVPAIVQDYLDGTVLMMAWMNRESLQKTMDTGRTWFWSRSRQE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHCCCCEEECCCHHH
LWPKGETSGHVQEVKSLRYDCDSDALLVGVEQIGDIACHTGERSCFHQIEGQIAAPPADT
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHH
LSQVFDVICDRKQNPSPDSYTCKLFAAGDNKILKKIGEESAEVVMACKDDNADEIAGEVA
HHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHCCCHHHEEEEECCCCHHHHHHHHH
DLIYHSLVALAHHDVDIKDVYRKLQERRR
HHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]