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The map label for this gene is lytC [H]

Identifier: 158335668

GI number: 158335668

Start: 2555059

End: 2556138

Strand: Reverse

Name: lytC [H]

Synonym: AM1_2518

Alternate gene names: 158335668

Gene position: 2556138-2555059 (Counterclockwise)

Preceding gene: 158335669

Following gene: 158335663

Centisome position: 39.3

GC content: 54.07

Gene sequence:

>1080_bases
TTGCCTGCTCAGAGACGACTGTGGAATTGCAAGATAAGGAAAGCCGGTTGGGTATTGCCCCTCGCTTTGAGTCTATTCTG
CTGGGCAGACAGTGCATCAGCGGGTCAACTGACCTACTGGAAGTTTAATTCTAAGCAAAGTCGCATTGACCTGATTACGA
ACTCAGCGACGAAGCCGCGGGCAAAAGTCGTCATGAACCCGACCCGGTTGGTGATTGACCTTCCCAACACCACCTTCCGT
AAACTCAAAGCCCGCCGCAAAATTAGCAAATATGTTCGAGAAGTGCGGGTCGCTCAGCATAATCGTAAGACCACGCGGGT
GGTAGTTGAGCTGAGTAAAAAATACACCTTGAGCCCCCGGCGAGTTCTGGTGCGGGGGATTGCCCCGAATCGCTGGTATG
TGCAACTGCCTAAATTTTTCCCCCTCAAGGATGCTAAAAAGTCGTCTCGGCGAACCATTGCCGTGCGAGTTCCGCCCCCC
AAAATCCCCAAGCCTTCTATTTCTTCACCGGGACCCATCGTTTCTAAACCCGTCAAATCCGGGGCAAAAGTCGTCGTCAT
TGATCCGGGCCATGGGGGGGCGGATCCAGGTGCCGTGGGCATTGGTGGTCTCCAGGAGAAACGAGTCGTCTTGGATGTGT
CGACGCAAGTGCATAACCTGCTGCGAAAGCGGGGGATTAATGCGGTGTTGACACGGACGGGCGATCGCGAAGTTGACTTA
CCGCCACGAGTGGCCAAAGCTGAAGGGGCTCGCGCCGACGTGTTTGTCAGTATTCATGCCAATGCCATTAGCCTGAGCCG
TCCTGAAGTCAACGGTTTAGAAACCTACTATTATGTGACGGGATACCGTTTGGCCCGCGCCATTCATACCAGCATTCGTC
GGACTGTATCCGTCGGCGATCGCGGCATTCGCCAAGCCCGCTTCTATGTCTTAAGAAAATCCTCCATGCCCGCCGCTCTC
GTGGAACTTGGGTTTGTAACGGGCAGCACTGATGCGGCAAAATTAAGAACAGCAGCCCATCGCAAACGGTTGGCCGAAGC
CATTGCCCAAGGCATCGTCAATTATCTTCAGGGCAGATAG

Upstream 100 bases:

>100_bases
ACGCATCATCTTTCTCTGGGCAAAACATCTGCCCAGGAAACTTGATCGCACTCCGACCAGTCAAGAGGGATTGACCCCCC
ACCTAATCGAGACTAAAGAT

Downstream 100 bases:

>100_bases
TCGGCAGACACCCTCCGGGCTAAGAGTTGAGTAGGGTGCGCAGAAGCTTTCCGGTCTTTAAATTCCAGACTTTGATACTG
CGATCGCTGCTCCCACTCAC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPAQRRLWNCKIRKAGWVLPLALSLFCWADSASAGQLTYWKFNSKQSRIDLITNSATKPRAKVVMNPTRLVIDLPNTTFR
KLKARRKISKYVREVRVAQHNRKTTRVVVELSKKYTLSPRRVLVRGIAPNRWYVQLPKFFPLKDAKKSSRRTIAVRVPPP
KIPKPSISSPGPIVSKPVKSGAKVVVIDPGHGGADPGAVGIGGLQEKRVVLDVSTQVHNLLRKRGINAVLTRTGDREVDL
PPRVAKAEGARADVFVSIHANAISLSRPEVNGLETYYYVTGYRLARAIHTSIRRTVSVGDRGIRQARFYVLRKSSMPAAL
VELGFVTGSTDAAKLRTAAHRKRLAEAIAQGIVNYLQGR

Sequences:

>Translated_359_residues
MPAQRRLWNCKIRKAGWVLPLALSLFCWADSASAGQLTYWKFNSKQSRIDLITNSATKPRAKVVMNPTRLVIDLPNTTFR
KLKARRKISKYVREVRVAQHNRKTTRVVVELSKKYTLSPRRVLVRGIAPNRWYVQLPKFFPLKDAKKSSRRTIAVRVPPP
KIPKPSISSPGPIVSKPVKSGAKVVVIDPGHGGADPGAVGIGGLQEKRVVLDVSTQVHNLLRKRGINAVLTRTGDREVDL
PPRVAKAEGARADVFVSIHANAISLSRPEVNGLETYYYVTGYRLARAIHTSIRRTVSVGDRGIRQARFYVLRKSSMPAAL
VELGFVTGSTDAAKLRTAAHRKRLAEAIAQGIVNYLQGR
>Mature_358_residues
PAQRRLWNCKIRKAGWVLPLALSLFCWADSASAGQLTYWKFNSKQSRIDLITNSATKPRAKVVMNPTRLVIDLPNTTFRK
LKARRKISKYVREVRVAQHNRKTTRVVVELSKKYTLSPRRVLVRGIAPNRWYVQLPKFFPLKDAKKSSRRTIAVRVPPPK
IPKPSISSPGPIVSKPVKSGAKVVVIDPGHGGADPGAVGIGGLQEKRVVLDVSTQVHNLLRKRGINAVLTRTGDREVDLP
PRVAKAEGARADVFVSIHANAISLSRPEVNGLETYYYVTGYRLARAIHTSIRRTVSVGDRGIRQARFYVLRKSSMPAALV
ELGFVTGSTDAAKLRTAAHRKRLAEAIAQGIVNYLQGR

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=418, Percent_Identity=26.0765550239234, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788776, Length=239, Percent_Identity=33.0543933054393, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1790611, Length=133, Percent_Identity=34.5864661654135, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 39869; Mature: 39738

Theoretical pI: Translated: 11.91; Mature: 11.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQRRLWNCKIRKAGWVLPLALSLFCWADSASAGQLTYWKFNSKQSRIDLITNSATKPR
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCC
AKVVMNPTRLVIDLPNTTFRKLKARRKISKYVREVRVAQHNRKTTRVVVELSKKYTLSPR
EEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
RVLVRGIAPNRWYVQLPKFFPLKDAKKSSRRTIAVRVPPPKIPKPSISSPGPIVSKPVKS
HEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCC
GAKVVVIDPGHGGADPGAVGIGGLQEKRVVLDVSTQVHNLLRKRGINAVLTRTGDREVDL
CCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCEEEEECCCCCCCC
PPRVAKAEGARADVFVSIHANAISLSRPEVNGLETYYYVTGYRLARAIHTSIRRTVSVGD
CCCHHHCCCCCEEEEEEEECCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
RGIRQARFYVLRKSSMPAALVELGFVTGSTDAAKLRTAAHRKRLAEAIAQGIVNYLQGR
HHHHHHEEEEEECCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PAQRRLWNCKIRKAGWVLPLALSLFCWADSASAGQLTYWKFNSKQSRIDLITNSATKPR
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCC
AKVVMNPTRLVIDLPNTTFRKLKARRKISKYVREVRVAQHNRKTTRVVVELSKKYTLSPR
EEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
RVLVRGIAPNRWYVQLPKFFPLKDAKKSSRRTIAVRVPPPKIPKPSISSPGPIVSKPVKS
HEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCC
GAKVVVIDPGHGGADPGAVGIGGLQEKRVVLDVSTQVHNLLRKRGINAVLTRTGDREVDL
CCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCEEEEECCCCCCCC
PPRVAKAEGARADVFVSIHANAISLSRPEVNGLETYYYVTGYRLARAIHTSIRRTVSVGD
CCCHHHCCCCCEEEEEEEECCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
RGIRQARFYVLRKSSMPAALVELGFVTGSTDAAKLRTAAHRKRLAEAIAQGIVNYLQGR
HHHHHHEEEEEECCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]