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The map label for this gene is dnaE [H]

Identifier: 158335662

GI number: 158335662

Start: 2545686

End: 2548280

Strand: Reverse

Name: dnaE [H]

Synonym: AM1_2512

Alternate gene names: 158335662

Gene position: 2548280-2545686 (Counterclockwise)

Preceding gene: 158335663

Following gene: 158335661

Centisome position: 39.18

GC content: 50.1

Gene sequence:

>2595_bases
ATGTCTTTTGTGGGGTTGCATATTCACAGCGACTATAGTCTCTTGGATGGAGCCAGTCAGCTCCCGGATCTGGTGTCCCG
AGCCGTTGAACTGAATATGCCCGCTATTGCCTTGACGGATCATGGGGTGATGTATGGGGCCATTGGCTTGATCAAAACCT
GCAAGAAGCAGGGGATTAAGCCGATTGTTGGCAATGAAATGTACGTGATCAATGGCGATATCACCAAGCAAGAGCGACGA
CCTCGGTATCACCAGGTGGTGTTGGCGAAGAATAAGAAGGGCTACCAGAACCTAGTTAAACTCACCACAATTTCTAGCCT
GCATGGGGTCCAGGGGCGGGGTATCTTCTCTCGACCCTGTATCAATAAAGATCTGCTAGAAGAGCACCACGAAGGACTCA
TCGTCACCAGCGCCTGCTTAGGGGGGGAGATTCCTCAGGCGATTATGAAGGAGAAGCCGGATATCGCCCGGCGAATTGCC
AAATGGTACAAAGAGCTTTTTGGAGATGACTATTACTTAGAAATTCAAGACCACGGTTCCCAGGAAGATCGGGTGGTCAA
CGTGGAATTGGTACGGATTGCCCAGGAACTTGATATTAAGGTGATTGCCACCAATGACTCCCATTTTATCTCCTGCTACG
ACGTGGAGTCCCATGATGCGTTGCTCTGTATCCAGACAGGCAAGCTGATTACGGAAGACAAGCGTCTGCGCTATAGCGGT
ACGGAGTATCTAAAAACTGCGGATGAGATGGCCCTGATGTTTCGGGACCATCTGACGGATGACGTGATTGCCGATGCGAT
CGCAACCACCCTAGAAGTGGCCGAAAAGATCGAAGCCTACGATATCTTTCGGGACCCCCAAAGCCCTGACTATGAAGTAC
CGGACGGCTATACAGCGGAAACCTACCTAGAAGAAGTGACCTGGGATGGCCTGTTAGAGCGCTGCAACTGCAAAGAACGA
TCCGAAGTCACGGATACCTATAAAGAGCGCCTGGAATATGAGCTGGAAATGCTACAGCAGATGGGCTTTTCCACCTATTT
CTTAGTGGTGTGGGATTACATCAAATATGCCCGCGACAATAATATTCCTGTAGGGCCGGGGCGAGGTAGTGCGGCAGGTT
CCCTGGTAGCCTATGCCTTAGGGATTACCAATATCGACCCAGTTCATCACGGTCTCCTGTTTGAGCGGTTCCTCAATCCA
GAACGGAAGTCTATGCCTGATATTGATACGGATTTCTGTATCGAAAAGCGGGACGCCATGATTGACTATGTGACCCGCAA
ATATGGCGAAGACCGGGTGGCTCAGATTATTACCTTTAACCGCATGACCTCCAAAGCGGTGCTCAAAGATGTGGCTCGGG
TGCTGGATATTCCCTATGGGGAAGCGGATCGGATGGCAAAGCTGATTCCGGTGGCTCGGGGTAAACCCACAAAGCTCAAG
GTGATGATCTCCGATGAAACACCTGCCCCAGAGTTTAAAGAAAAATACGATAAAGATCCGATTGTGCGTCGCTGGCTAGA
TATGGCGATTCGGATTGAGGGTACTAACAAAACCTTTGGGGTGCATGCGGCAGGAGTGGTGATTGCCTCGGAACCCTTGG
ATCAGATTGTGCCTTTGCAGCGCAATAACGATGGGGCGGTGATTACCCAATACTTTATGGAGGATCTAGAGTCTTTGGGT
CTGCTGAAAATGGACTTCTTGGGCCTCAAGAACCTGACAATGATCCAGAAAACCACGGATCTGCTGAAGCAAAATCGCCA
GCTCGATCTAGACGTAGATCGAGTGCCCATGGATGATCCAGAAGCCTTCCAGCTCCTAGCACGAGGGGAACTAGAAGGGG
TCTTCCAGTTGGAGTCCTCGGGGATGCGGCAGGTGGTGAAAGACCTCAAGCCCTCCAATATTGAGGATATTTCTTCGATT
CTGGCCCTCTATCGACCGGGGCCGTTGGATGCGGGGCTGATTCCCAAGTTTATTAACCGTAAGCATGGTCGCGAAAGTAT
TGACTATCCCCATGATCTGATTAAGCCGATTCTGGAAGAAACTTACGGAATTATGGTCTATCAAGAGCAAATTATGAAGA
TTGCCCAGGATATGGCGGGATATTCCCTGGGACAAGCCGATTTGCTGCGGCGAGCCATGGGTAAAAAGAAGATGGCGGAG
ATGGAGAAGCATCGCGAGATCTTTATTGATGGCGCTGCTGAAAAAGGGGTCGATAAGAAGGTCTCGGAAAACCTCTTCGA
CCAAATGCTGTTATTTGCGGAATATTGCCTGAGCTATGACACGCCTGTATTGACTTTGGAGTATGGTTGGCTGCCCATTG
GTCAGGTGGTGCAGGAGCAAATTGAGTGTCAGGTGTTTTCGATTAATGAGCGAGGGCATCTGTATACGCAACCGATTGCT
CAGTGGCATCATCGGGGTCAGCAAGAGGTGTTTGAGTATACCCTGGCGGATGGTTCGACGATTCAGGCGACGGCGGAACA
TCAGTTTATGACGACGGATGGTCAGATGTATCCGGTTCAGCAAATTTTTGAGGAAGGGTTAAGTTTGAAGCAGTTGCCCT
TGCCTTGGCAGTCAGGTTTACAACTAGCTAGCTAA

Upstream 100 bases:

>100_bases
GAAATCCCTATGCAGTGGGCAATCACAAATAAGGTTGAGACTGTAGTCGTTGCGATCGCAATCCGTTACCCTATAGCCAG
GTTTAGCAAGGATGTGGCCA

Downstream 100 bases:

>100_bases
GATGAGTAGCGCTTATTCTATGATGAACGAGTAGAATGATCCTAATTTTTCTATATGTAATTTAAAGTACACCCTGTACT
AGGTTTAATGTGGAAGGTAT

Product: DNA polymerase III subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 864; Mature: 863

Protein sequence:

>864_residues
MSFVGLHIHSDYSLLDGASQLPDLVSRAVELNMPAIALTDHGVMYGAIGLIKTCKKQGIKPIVGNEMYVINGDITKQERR
PRYHQVVLAKNKKGYQNLVKLTTISSLHGVQGRGIFSRPCINKDLLEEHHEGLIVTSACLGGEIPQAIMKEKPDIARRIA
KWYKELFGDDYYLEIQDHGSQEDRVVNVELVRIAQELDIKVIATNDSHFISCYDVESHDALLCIQTGKLITEDKRLRYSG
TEYLKTADEMALMFRDHLTDDVIADAIATTLEVAEKIEAYDIFRDPQSPDYEVPDGYTAETYLEEVTWDGLLERCNCKER
SEVTDTYKERLEYELEMLQQMGFSTYFLVVWDYIKYARDNNIPVGPGRGSAAGSLVAYALGITNIDPVHHGLLFERFLNP
ERKSMPDIDTDFCIEKRDAMIDYVTRKYGEDRVAQIITFNRMTSKAVLKDVARVLDIPYGEADRMAKLIPVARGKPTKLK
VMISDETPAPEFKEKYDKDPIVRRWLDMAIRIEGTNKTFGVHAAGVVIASEPLDQIVPLQRNNDGAVITQYFMEDLESLG
LLKMDFLGLKNLTMIQKTTDLLKQNRQLDLDVDRVPMDDPEAFQLLARGELEGVFQLESSGMRQVVKDLKPSNIEDISSI
LALYRPGPLDAGLIPKFINRKHGRESIDYPHDLIKPILEETYGIMVYQEQIMKIAQDMAGYSLGQADLLRRAMGKKKMAE
MEKHREIFIDGAAEKGVDKKVSENLFDQMLLFAEYCLSYDTPVLTLEYGWLPIGQVVQEQIECQVFSINERGHLYTQPIA
QWHHRGQQEVFEYTLADGSTIQATAEHQFMTTDGQMYPVQQIFEEGLSLKQLPLPWQSGLQLAS

Sequences:

>Translated_864_residues
MSFVGLHIHSDYSLLDGASQLPDLVSRAVELNMPAIALTDHGVMYGAIGLIKTCKKQGIKPIVGNEMYVINGDITKQERR
PRYHQVVLAKNKKGYQNLVKLTTISSLHGVQGRGIFSRPCINKDLLEEHHEGLIVTSACLGGEIPQAIMKEKPDIARRIA
KWYKELFGDDYYLEIQDHGSQEDRVVNVELVRIAQELDIKVIATNDSHFISCYDVESHDALLCIQTGKLITEDKRLRYSG
TEYLKTADEMALMFRDHLTDDVIADAIATTLEVAEKIEAYDIFRDPQSPDYEVPDGYTAETYLEEVTWDGLLERCNCKER
SEVTDTYKERLEYELEMLQQMGFSTYFLVVWDYIKYARDNNIPVGPGRGSAAGSLVAYALGITNIDPVHHGLLFERFLNP
ERKSMPDIDTDFCIEKRDAMIDYVTRKYGEDRVAQIITFNRMTSKAVLKDVARVLDIPYGEADRMAKLIPVARGKPTKLK
VMISDETPAPEFKEKYDKDPIVRRWLDMAIRIEGTNKTFGVHAAGVVIASEPLDQIVPLQRNNDGAVITQYFMEDLESLG
LLKMDFLGLKNLTMIQKTTDLLKQNRQLDLDVDRVPMDDPEAFQLLARGELEGVFQLESSGMRQVVKDLKPSNIEDISSI
LALYRPGPLDAGLIPKFINRKHGRESIDYPHDLIKPILEETYGIMVYQEQIMKIAQDMAGYSLGQADLLRRAMGKKKMAE
MEKHREIFIDGAAEKGVDKKVSENLFDQMLLFAEYCLSYDTPVLTLEYGWLPIGQVVQEQIECQVFSINERGHLYTQPIA
QWHHRGQQEVFEYTLADGSTIQATAEHQFMTTDGQMYPVQQIFEEGLSLKQLPLPWQSGLQLAS
>Mature_863_residues
SFVGLHIHSDYSLLDGASQLPDLVSRAVELNMPAIALTDHGVMYGAIGLIKTCKKQGIKPIVGNEMYVINGDITKQERRP
RYHQVVLAKNKKGYQNLVKLTTISSLHGVQGRGIFSRPCINKDLLEEHHEGLIVTSACLGGEIPQAIMKEKPDIARRIAK
WYKELFGDDYYLEIQDHGSQEDRVVNVELVRIAQELDIKVIATNDSHFISCYDVESHDALLCIQTGKLITEDKRLRYSGT
EYLKTADEMALMFRDHLTDDVIADAIATTLEVAEKIEAYDIFRDPQSPDYEVPDGYTAETYLEEVTWDGLLERCNCKERS
EVTDTYKERLEYELEMLQQMGFSTYFLVVWDYIKYARDNNIPVGPGRGSAAGSLVAYALGITNIDPVHHGLLFERFLNPE
RKSMPDIDTDFCIEKRDAMIDYVTRKYGEDRVAQIITFNRMTSKAVLKDVARVLDIPYGEADRMAKLIPVARGKPTKLKV
MISDETPAPEFKEKYDKDPIVRRWLDMAIRIEGTNKTFGVHAAGVVIASEPLDQIVPLQRNNDGAVITQYFMEDLESLGL
LKMDFLGLKNLTMIQKTTDLLKQNRQLDLDVDRVPMDDPEAFQLLARGELEGVFQLESSGMRQVVKDLKPSNIEDISSIL
ALYRPGPLDAGLIPKFINRKHGRESIDYPHDLIKPILEETYGIMVYQEQIMKIAQDMAGYSLGQADLLRRAMGKKKMAEM
EKHREIFIDGAAEKGVDKKVSENLFDQMLLFAEYCLSYDTPVLTLEYGWLPIGQVVQEQIECQVFSINERGHLYTQPIAQ
WHHRGQQEVFEYTLADGSTIQATAEHQFMTTDGQMYPVQQIFEEGLSLKQLPLPWQSGLQLAS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=772, Percent_Identity=41.0621761658031, Blast_Score=573, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR003587
- InterPro:   IPR006141
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98140; Mature: 98008

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50817 INTEIN_N_TER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVGLHIHSDYSLLDGASQLPDLVSRAVELNMPAIALTDHGVMYGAIGLIKTCKKQGIK
CCEEEEEEECCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
PIVGNEMYVINGDITKQERRPRYHQVVLAKNKKGYQNLVKLTTISSLHGVQGRGIFSRPC
CCCCCEEEEEECCCCCHHCCCCHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
INKDLLEEHHEGLIVTSACLGGEIPQAIMKEKPDIARRIAKWYKELFGDDYYLEIQDHGS
CCHHHHHHHCCCEEEEEHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCC
QEDRVVNVELVRIAQELDIKVIATNDSHFISCYDVESHDALLCIQTGKLITEDKRLRYSG
CCCEEEEHHHHHHHHHCCEEEEEECCCCEEEEEEECCCCEEEEEECCCEECCCCCCEECC
TEYLKTADEMALMFRDHLTDDVIADAIATTLEVAEKIEAYDIFRDPQSPDYEVPDGYTAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
TYLEEVTWDGLLERCNCKERSEVTDTYKERLEYELEMLQQMGFSTYFLVVWDYIKYARDN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
NIPVGPGRGSAAGSLVAYALGITNIDPVHHGLLFERFLNPERKSMPDIDTDFCIEKRDAM
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHH
IDYVTRKYGEDRVAQIITFNRMTSKAVLKDVARVLDIPYGEADRMAKLIPVARGKPTKLK
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEE
VMISDETPAPEFKEKYDKDPIVRRWLDMAIRIEGTNKTFGVHAAGVVIASEPLDQIVPLQ
EEECCCCCCCHHHHHCCCCHHHHHHHCEEEEEECCCCEEEEEECEEEEECCCHHHHCCCE
RNNDGAVITQYFMEDLESLGLLKMDFLGLKNLTMIQKTTDLLKQNRQLDLDVDRVPMDDP
ECCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
EAFQLLARGELEGVFQLESSGMRQVVKDLKPSNIEDISSILALYRPGPLDAGLIPKFINR
HHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
KHGRESIDYPHDLIKPILEETYGIMVYQEQIMKIAQDMAGYSLGQADLLRRAMGKKKMAE
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHH
MEKHREIFIDGAAEKGVDKKVSENLFDQMLLFAEYCLSYDTPVLTLEYGWLPIGQVVQEQ
HHHHHEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
IECQVFSINERGHLYTQPIAQWHHRGQQEVFEYTLADGSTIQATAEHQFMTTDGQMYPVQ
HCEEEEEECCCCCEEHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCEEEECCCCCCCHH
QIFEEGLSLKQLPLPWQSGLQLAS
HHHHCCCCEECCCCCHHCCCCCCC
>Mature Secondary Structure 
SFVGLHIHSDYSLLDGASQLPDLVSRAVELNMPAIALTDHGVMYGAIGLIKTCKKQGIK
CEEEEEEECCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
PIVGNEMYVINGDITKQERRPRYHQVVLAKNKKGYQNLVKLTTISSLHGVQGRGIFSRPC
CCCCCEEEEEECCCCCHHCCCCHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
INKDLLEEHHEGLIVTSACLGGEIPQAIMKEKPDIARRIAKWYKELFGDDYYLEIQDHGS
CCHHHHHHHCCCEEEEEHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCC
QEDRVVNVELVRIAQELDIKVIATNDSHFISCYDVESHDALLCIQTGKLITEDKRLRYSG
CCCEEEEHHHHHHHHHCCEEEEEECCCCEEEEEEECCCCEEEEEECCCEECCCCCCEECC
TEYLKTADEMALMFRDHLTDDVIADAIATTLEVAEKIEAYDIFRDPQSPDYEVPDGYTAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
TYLEEVTWDGLLERCNCKERSEVTDTYKERLEYELEMLQQMGFSTYFLVVWDYIKYARDN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
NIPVGPGRGSAAGSLVAYALGITNIDPVHHGLLFERFLNPERKSMPDIDTDFCIEKRDAM
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHH
IDYVTRKYGEDRVAQIITFNRMTSKAVLKDVARVLDIPYGEADRMAKLIPVARGKPTKLK
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEE
VMISDETPAPEFKEKYDKDPIVRRWLDMAIRIEGTNKTFGVHAAGVVIASEPLDQIVPLQ
EEECCCCCCCHHHHHCCCCHHHHHHHCEEEEEECCCCEEEEEECEEEEECCCHHHHCCCE
RNNDGAVITQYFMEDLESLGLLKMDFLGLKNLTMIQKTTDLLKQNRQLDLDVDRVPMDDP
ECCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
EAFQLLARGELEGVFQLESSGMRQVVKDLKPSNIEDISSILALYRPGPLDAGLIPKFINR
HHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
KHGRESIDYPHDLIKPILEETYGIMVYQEQIMKIAQDMAGYSLGQADLLRRAMGKKKMAE
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHH
MEKHREIFIDGAAEKGVDKKVSENLFDQMLLFAEYCLSYDTPVLTLEYGWLPIGQVVQEQ
HHHHHEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
IECQVFSINERGHLYTQPIAQWHHRGQQEVFEYTLADGSTIQATAEHQFMTTDGQMYPVQ
HCEEEEEECCCCCEEHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCEEEECCCCCCCHH
QIFEEGLSLKQLPLPWQSGLQLAS
HHHHCCCCEECCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 9689062; 10734038; 11170467 [H]